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Method for collecting high-quality patinopecten yessoensis seminal fluid

A technique for collecting scallops and scallops, which is applied in the field of marine biology, can solve the problems of unsatisfactory freezing effect of oyster sperm, cannot ensure complete sperm maturity, cannot avoid sperm activation, etc., and achieves the promotion of multiple mature discharges, easy operation, and high quality. Good results

Active Publication Date: 2014-07-02
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most shellfish semen are collected by dissection and natural discharge. The former cannot guarantee the complete maturity of the obtained sperm, and the latter cannot prevent the sperm from being activated by seawater prematurely.
For example, the collection of abalone semen usually adopts a combination of drying in the shade and heating to induce ejaculation of abalone, and then centrifuges to concentrate the sperm produced in seawater [Liliana Salinas-Flores et al., Journal of shellfish, 2005, 24 (2): 415-420; Gwo Jin-Chywan et al., Theriogenology, 2002, 58: 1563-1578], the obtained spermatozoa have a relatively large energy loss due to long-term contact with seawater; Hughes (Cryobiology, 1973, 10: 342-344) and Zell et al. (Cryobiology, 1979, 16:448-460) studies have shown that this seawater-sperm mixture is not ideal for the freezing of oyster sperm. Although the method of dissecting gonad tissue is used to obtain a high concentration of semen, it cannot guarantee that the obtained sperm are fully mature, and There are many other tissue cells left in the semen, and the mother can only collect the sperm once

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0024] While using the method of the present invention to collect the sperm of Ezo scallop male shellfish, adopt 2 kinds of different methods to collect semen in addition: A. Dissection method: cut the gonads into pieces and put them on a 500-mesh sieve silk, wash and filter out with sea water Put the semen into a small beaker pre-cooled on ice; B. Centrifugal concentration method: collect the semen discharged into seawater with a centrifuge tube, freeze and centrifuge, and store the concentrated semen in a 10ml centrifuge tube to pre-cool on ice for later use. In order to eliminate the differences among individual scallops, the semen collected by the two methods in this example and Example 1 all came from multiple individuals of the same batch of scallops.

[0025] The sperm motility collected by the dissection method and the centrifugal concentration method was detected by the same method as in step 3) of Example 1, and the results showed that the motility of the sperm in the...

Embodiment 3

[0026] Example 3 Verify the semen quality collected by 3 methods after detection:

[0027]Sperm cryopreservation and insemination experiments were conducted to verify the relationship between semen collection methods and the quality of collected semen. The results show that the motility difference of spermatozoa before the freezing of the semen collected by the three methods (the method of Example 1 and the two methods of Example 2) is not significant, but the freezing effect of the seminal fluid collected by the method of Example 1 after freezing is the best. The motility of sperm was 49.3±6.1%, while the motility of frozen sperm obtained in A and B was 25.7±4.0% and 28.7±1.5%, respectively, which was significantly different from the result of Example 1 (p<0.05). Because the surrounding environment of the sperm naturally discharged from the kidney hole is closer to the physiological state, and the higher sperm density limits the sperm activity, the sperm energy is better pres...

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Abstract

The invention discloses a method for collecting high-quality patinopecten yessoensis seminal fluid, and belongs to the technical field of marine organisms. The method comprises the first step of selecting, temporally rearing and maturing the high-quality male patinopecten yessoensis, the second step of luring the male patinopecten yessoensis to discharge the seminal fluid and collecting the seminal fluid many times, and the third step of detecting the quality of the collected seminal fluid. The collecting method is simple and easy to operate, the parent patinopecten yessoensis cannot be hurt, the seminal fluid can be repeatedly collected, and the collected seminal fluid can be stored for a long time. It is only required to understand the genital glands of the scallops and the anatomical structures of the excretory organs and accurately recognize the nephridium and the nephridiopore when the seminal fluid is collected. The seminal liquid collected from one ordinary male patinopecten yessoensis each time can be 0.5-1.5 ml. Under the situations that the temperature is controlled and the sufficient nutrient is guaranteed, the gonad can be promoted to be mature and discharge the seminal fluid many times, and the seminal fluid of each patinopecten yessoensis can be repeatedly collected two to four times till the March or April.

Description

technical field [0001] The invention belongs to the technical field of marine biology, belongs to the technological improvement in the genetic breeding research of shellfish, and in particular relates to a method for collecting high-quality scallop semen. Background technique [0002] The collection of aquatic animal semen generally refers to the technical method of obtaining semen as the research object in the research of artificial insemination in the breeding of improved breeds and artificial seedlings, or in the research of reproductive isolation. [0003] The reproductive organ of the male Ezo scallop is a half-lunate gonad located on the ventral surface, and the excretory organ is a pair of kidneys, each of which is composed of a spongy gland and a thin-walled short tubular body with cilia. The mouth opens in the pericardial cavity; the latter's kidney opening (cloaca) opens in the mantle cavity, attached to the front of the adductor muscle, between the gonads and the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 杨爱国周丽青杨培民刘志鸿吴彪孙秀俊
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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