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38results about How to "Avoid crossbreeding" patented technology

Multi-fluorescence immunity analysis method for quickly distinguishing PEDV, TGEV and PoRV

The invention discloses a multi-fluorescence immunity analysis method for quickly distinguishing PEDV, TGEV and PoRV. The method is easy to operate, a target amplified fragment is obtained through PCR, then hybridization is conducted on an amplified product, fluorescence coded microspheres and streptavidin-phycoerythrin, the MFi value is read through a detection instrument, and different types of viruses are distinguished. By means of the method, porcine epizootic diarrhea, swine transmissible gastroenteritis and pig group A rotavirus can be accurately detected at the same time, the specificity is high, the sensitivity is high, and the repeatability is good. Compared with a traditional detection method, various molecules of different purposes in the same sample are detected at the same time, the sample consumption is little, operation is simple and fast, and the detection cost can be greatly lowered.
Owner:GUANGDONG LAB ANIMALS MONITORING INST

SNP locus related to growth characteristics of patinopecten yessoensis and detection and application thereof

The invention provides an SNP locus related to growth characteristics of patinopecten yessoensis. The locus is the 1054 site of IGFBP (Insulin Like Growth Factor Binding Proteins) genes of which the nucleotide sequence is shown as SEQ ID NO:2 and has the base of A or G. The invention also provides a probe for detecting the SNP locus and a parting primer. The transcription sequence of the IGFBP genes in the patinopecten yessoensis is subjected to sequencing and Clustal comparison, and three SNP loci are screened. The site polymorphism is detected in the patinopecten yessoensis group by using a high resolution melting curve technology, and correlation between the site genotype frequency and the growth characteristics of patinopecten yessoensis is analyzed. The locus C.1054A>G is obviously related to important growth characteristics such as the height, shell length, weight, soft weight and adductor muscle weight of the patinopecten yessoensis, the growth characteristics of the AG type individuals are obviously lower than those of AA and GG type individuals (P is less than 0.05), and the characteristic value of the GG type is the highest. Therefore, individuals of which the genotype is GG on the locus can be preferentially selected during production and are used as parents for performing high-yield patinopecten yessoensis breeding or performing large-scale breeding.
Owner:OCEAN UNIV OF CHINA

Multiple liquid phase gene chip method and reagent for rapidly detecting guinea pig LCMV, SV, PVM and Reo-3 viruses

The invention discloses a multiple liquid phase gene chip method and a reagent for rapidly detecting guinea pig LCMV, SV, PVM and Reo-3 viruses. The operation is simple. A target amplified fragment is acquired through PCR, and then the amplified product, fluorescent coding micro-balloon and streptavidin-phycoerythrin are hybridized, MFI value is read through a tester and different viruses are distinguished. According to the method provided by the invention, the rat lymphocyte choriomeningitis virus, sendai virus, rat pneumonia virus and Reo-3 virus can be accurately detected at the same time; the specificity is strong, the sensitivity is high and the repeatability is excellent; compared with the traditional detection method, the method has the advantages that different target molecules in a same sample can be detected, the detection flux is high, the sample dosage is less, the operation is simple and quick, the detection cost is greatly reduced and the detection efficiency is increased.
Owner:GUANGDONG LAB ANIMALS MONITORING INST

Multiple fluoroimmunoassay primer, kit and method for rapidly distinguishing 5 avian immunosuppression pathogens

The invention discloses a multiple fluoroimmunoassay primer, kit and method for rapidly distinguishing 5 avian immunosuppression pathogens. Operation is simple, a target amplified fragment is acquired through a PCR, an amplified product, fluorescence coded microspheres and streptavidin-phycoerythrin are hybridized, the MFI value is read through a detector, and viruses of different types are distinguished. The method can simultaneously detect the chicken infectious anemia virus, the chicken Marek's disease virus, the avian reticuloendotheliosis virus, the avian reovirus and the chicken infectious bursa disease virus accurately, specificity is high, sensitivity is high, and repeatability is good. Compared with a traditional detection method, the method achieves simultaneous detection of multiple different target molecules in the same sample, the use number of samples is small, operation is simple and rapid, and the detection cost can be greatly reduced.
Owner:GUANGDONG LAB ANIMALS MONITORING INST

Multi-liquid-phase gene chip detection primer, kit and method for quickly distinguishing 5 respiratory pathogens of mouse

The invention discloses a multi-liquid-phase gene chip detection primer, kit and method for quickly distinguishing 5 respiratory pathogens of a mouse. The multi-liquid-phase gene chip detection primer is easy to operate; a target amplified fragment is obtained through PCR (Polymerase Chain Reaction); an amplified product, fluorescence coded microspheres and streptavidin-phycoerythrin are hybridized; an MFI (Mean Fluorescence Intensity) value is read through a detector so as to distinguish different types of viruses. The method disclosed by the invention can detect a pneumonia virus, a hantaan virus, a sendai virus, a lymphocytic choriomeningitis virus and mycoplasma pulmonis of the mouse at the same time, and is high in specificity, high in sensitivity and high in repetitiveness. Compared with the conventional detection method, the method disclosed by the invention realizes simultaneous detection of various different target molecules in the same sample; the sample use amount is small; the operation is simple and quick; the detection cost can be greatly reduced.
Owner:GUANGDONG LAB ANIMALS MONITORING INST

Multiple fluorescence immunoassay primer, kit and method capable of fast distinguishing CAV, MDV, REV and IBDV

The invention discloses a multiple fluorescence immunoassay primer, kit and method capable of fast distinguishing CAV, MDV, REV and IBDV. The multiple fluorescence immunoassay primer, kit and method has the advantages that target amplification fragments are obtained through PCR, \ amplification products, fluorescence encoded microspheres and streptavidin-phycoerythrin are hybridized, MFI values are read through a detector, and different types of pathogens are distinguished; the method can simultaneously and accurately detect chicken infectious anemia viruses, chicken Marek's disease viruses, chicken reticuloendotheliosis viruses and chicken infectious bursal disease viruses and is high in specificity, high in sensitivity and good in repeatability; compared with a traditional method, the method can simultaneously detect various molecules, with different purposes, in the same sample and is low in sample use amount, simple and fast to operate, capable of greatly lowering detecting cost, good in flexibility and capable of increasing and decreasing to-be-detected pathogen types on the basis.
Owner:GUANGDONG LAB ANIMALS MONITORING INST

Gene synthesis process, gene chip and kit

Provided is a chip process of gene synthesis, and the process comprises incorporating the whole procedure, which comprises amplifying oligonucleotides and assembling the oligonucleotides into a gene in parallel, onto a single chip. A specific mismatch endonuclease is also used in the process to establish an error repair system in gene synthesis, and the error rate is decreased to about 0.19 mismatched bases / kb. The high-throughput, high-fidelity and low-cost chip process of gene synthesis provided in the present invention can meet the requirements of gene synthesis and the optimization and screening of protein expression on a large scale at the frontier of life sciences such as synthetic biology, genomics, and systems biology.
Owner:DONGXUAN GENE TECH CO LTD

Multiplex fluorescence immunoassay primer, kit and method for rapidly distinguishing four respiratory pathogens

The invention discloses a multiplex fluorescence immunoassay primer, kit and method for rapidly distinguishing four respiratory pathogens. Operation is easy, a target amplified fragment is obtained through a PCR, then an amplified product, fluorescence coded microspheres and streptavidin-phycoerythrin are hybridized, an MFI value is read through a detector, and viruses of different types are distinguished. The method can be used for accurately detecting chicken new castle disease viruses, avian influenza viruses, chicken infectious bronchitis viruses and chicken infectious laryngotracheitis viruses at the same time, and is high in specificity, high in sensitivity and good in repeatability. Compared with a traditional detection method, the method can detect multiple different target molecules in the same sample at the same time, the use quantity of samples is small, operation is easy and rapid, and the detection cost can be greatly reduced. The flexibility is good, and the varieties of detected pathogens can be increased or decreased on the basis according to requirements.
Owner:GUANGDONG LAB ANIMALS MONITORING INST

Multi-immunofluorescence assay primers, kit and method for detecting chicken Marek's disease viruses and chicken infectious anemia viruses

The invention discloses multi-immunofluorescence assay primers, a kit and a method for detecting chicken Marek's disease viruses and chicken infectious anemia viruses. The primers, kit and method are simple in operation. The method comprises acquiring a target amplified fragment by PCR, then hybridizing the amplified product, fluorescence-coded microspheres and streptavidin-phycoerythrin, then reading an MFI value through a detector and distinguishing the different types of pathogens. The method can simultaneously detect the chicken Marek's disease viruses and chicken infectious anemia viruses, has the advantages of strong specificity, high sensitivity and good reproducibility, and can realize simultaneous detection of many different target molecules in the same sample. The multi-immunofluorescence assay primers have good flexibility and can detect more or less types of pathogens on the basis of the demands.
Owner:GUANGDONG LAB ANIMALS MONITORING INST

Amplification method for 3'-RACE adaptor primer and 3'-end unknown gene sequences

The invention discloses an amplification method for 3'-RACE adaptor primer and 3'-end unknown gene sequences. The 3'-RACE adaptor primer is of a stem-loop structure composed of a stem handle region, astem-loop region and a PolyA binding region; the stem handle region and the stem-loop region have a nucleotide sequence shown in SEQ ID NO.1; and 15, 16, 17, 18, 19 or 20 continuous thymine are designed in the PolyA binding region. Two fragments of the stem handle region of the 3'-RACE adaptor primer have complementary sequence base so as to form a handle of the stem loop. Due to base stacking atthe handle, the stability of the primer is increased, and the reverse transcription efficiency is improved. A stem-loop configuration is formed due to a double-chain structure and self-complementaryproperty, so that hybridization between a reverse transcription primer and a target RNA template can be avoided, and background interference is reduced. More G / C bases are particularly inserted into the stem-loop region of the 3'-RACE adaptor primer, so that the annealing temperature is increased, and the non-specific PCR amplification is reduced.
Owner:FISHERIES RES INST ANHUI ACAD OF AGRI SCI

Automatic power-off take-up charging pile

The invention discloses an automatic power-off take-up charging pile, and the charging pile comprises a charging rack; the left side of the charging rack is provided with a winding space, the front side of the winding space is provided with a monitoring space, the inner wall of the front side wall of the monitoring space is spherically connected with a detector, and the rear side wall of the monitoring space is fixedly connected with a winding motor. The front side of the winding motor is in power connection with a winding power shaft, and the winding power shaft is fixedly connected with a winding gear. According to the automatic power-off take-up charging pile provided by the invention, whether an electric vehicle is fully charged or not is determined through the change of the current in the charging wire, and after charging is completed, the electric wire is taken back to the charging pile to be used by a next electric vehicle; therefore, the problem that the service life of a battery of an electric vehicle is affected due to long charging time is effectively avoided; meanwhile, charging efficiency is improved, the use efficiency of the charging pile is greatly improved, wires are automatically collected, time is saved for people, and potential safety hazards caused by excessive mixing of lines are avoided.
Owner:深圳市好立得商贸有限公司

Double T-DNA vector capable of achieving agrobacterium co-transformation and establishment method and application thereof

The invention provides a double T-DNA vector capable of achieving agrobacterium co-transformation. The double T-DNA vector is obtained by serially connecting a reporter gene GUS and a resistant selection marker gene, serially connecting a fluorescence reporter gene and a target gene, then respectively connecting the two serially connected sequences to two independent T-DNA areas and establishing the sequences to a plant binary expression vector. The vector is utilized to cultivate transgenic plants without selection markers and can quickly test positive transgenic plants from offspring.
Owner:SICHUAN AGRI UNIV

Multiplex fluorescence analysis method for simultaneous detection of four rat parvoviruses and kit

The invention discloses a multiplex fluorescence analysis method for simultaneous detection of four rat parvoviruses and a kit. The method is simple to operate. By PCR, a target amplified fragment is obtained; then, the amplified product, fluorescence coded microspheres and streptavidin-phycoerythrin are hybridized; and through a detector, MFI value is read to distinguish pathogenies of different types. The method can simultaneously detect and identify rat parvoviruses RMV, KRV, H-1 and PRV-1a, has advantages of strong specificity, high sensitivity, good repeatability and the like, and can realize simultaneous detection of various different target molecules in a same sample. The method has good flexibility. Types of detected pathogenies can be increased or reduced according to needs.
Owner:GUANGDONG LAB ANIMALS MONITORING INST

Yarn impurity removing device in warp guide device of warp knitting machine

ActiveCN107557986AReduce the chance of breakingPlay a positioning roleWarp knittingLubricationWarp knitting
The invention provides a yarn impurity removing device in a warp guide device of a warp knitting machine, and belongs to the technical field of machines. The warp guide device of the warp knitting machine comprises a machine frame and a tank arranged on the machine frame, wherein a lubricating shaft is rotationally arranged in the tank. The yarn impurity removing device comprises an air pipe arranged above the tank, and the axial direction of the air pipe is consistent with the axial direction of the lubricating shaft; the two ends of the air pipe are a closed end and an opened end respectively, a plurality of exhaust holes are formed in the lower side of the air pipe in a penetrating mode, and the exhaust holes are aligned with the lubrication shaft; a fan is further fixedly arranged on the machine frame, the fan comprises a straight tubular air outlet part, and a stainless steel corrugated pipe making the opened end and the air outlet part communicated is arranged between the openedend and the air outlet part; an adjusting pipe and a connecting column are vertically arranged on the lower portion of the closed end, and the lower end of the adjusting pipe is fixedly connected withthe machine frame; the upper end of the connecting column is fixedly connected with the closed end, the lower end of the connecting column is inserted into the adjusting pipe, and a detachable locking mechanism for fixing the connecting column and the adjusting pipe together is arranged between the connecting column and the adjusting pipe. The yarn impurity removing device has the advantage thatyarn crossing can be prevented.
Owner:海宁市成达经编股份有限公司

A multiplex fluorescent gene detection method for rapidly distinguishing ect, mad, mcmv, poly

The invention discloses a multiplex fluorescence gene detection method for quickly distinguishing ECT, MAD, MCMV and POLY. The method is simple in operation and a target amplification fragment is obtained through PCR. After that, an amplification product, fluorescent encoding microspheres and streptavidin-phycoerythrin are subjected to hybridization, and then an MFI value is read by a detector. In this way, different types of viruses can be distinguished. According to the technical scheme of the invention, the method is good in sensitivity, accuracy, repeatability and specificity, and simple, convenient and quick to use. Compared with the conventional detection methods, the above method realizes the simultaneous detection of different target fragments in the same sample. Meanwhile, the method has the advantages of small sample amount, and simple and rapid operation. The detection cost can be greatly reduced.
Owner:GUANGDONG LAB ANIMALS MONITORING INST

A multiple liquid-phase gene chip primer, kit and analysis method for simultaneously detecting seven aminoglycosamine drug-resistant genes

The invention discloses a multiple liquid-phase gene chip primer, a kit and an analysis method for simultaneously detecting seven aminoglycoside drug-resistant genes; (6')-Ie-aph(2")-Ia, aph(3')-IIIa, ant(4')-Ia, ant(9)-Ia, aadE, aph(3')-Ia and aph( 3")-Ib, and has high sensitivity and high accuracy, which can realize the simultaneous detection of multiple different target molecules in the same sample. Primers, kits and analysis methods; its technical scheme includes primer nucleotide sequences such as SEQ ID NO: 1-The primer shown in SEQ ID NO: 4; the invention belongs to the field of pathogen detection of experimental animals.
Owner:GUANGDONG LAB ANIMALS MONITORING INST

Yarn impurity removal device in the warp guide device of warp knitting machine

The invention provides a yarn impurity removing device in a warp guide device of a warp knitting machine, and belongs to the technical field of machines. The warp guide device of the warp knitting machine comprises a machine frame and a tank arranged on the machine frame, wherein a lubricating shaft is rotationally arranged in the tank. The yarn impurity removing device comprises an air pipe arranged above the tank, and the axial direction of the air pipe is consistent with the axial direction of the lubricating shaft; the two ends of the air pipe are a closed end and an opened end respectively, a plurality of exhaust holes are formed in the lower side of the air pipe in a penetrating mode, and the exhaust holes are aligned with the lubrication shaft; a fan is further fixedly arranged on the machine frame, the fan comprises a straight tubular air outlet part, and a stainless steel corrugated pipe making the opened end and the air outlet part communicated is arranged between the openedend and the air outlet part; an adjusting pipe and a connecting column are vertically arranged on the lower portion of the closed end, and the lower end of the adjusting pipe is fixedly connected withthe machine frame; the upper end of the connecting column is fixedly connected with the closed end, the lower end of the connecting column is inserted into the adjusting pipe, and a detachable locking mechanism for fixing the connecting column and the adjusting pipe together is arranged between the connecting column and the adjusting pipe. The yarn impurity removing device has the advantage thatyarn crossing can be prevented.
Owner:海宁市成达经编股份有限公司

An Air Heat Collector with Vacuum Tubes Connectable in Series

The invention provides an air heat collector capable of being spliced to vacuum tubes in series connection, and belongs to the technical field of solar energy. The air heat collector comprises an airheader and vacuum heat collection tubes. An air dispersion tub is arranged in the air header. A plurality of equidistantly-arrayed air feed inlets are arranged at the lower end of the air dispersion tube. A plurality of equidistantly-arrayed connectors are arranged at the lower end of the air header. One end of each vacuum heat collection tube is closed, and the other ends of the vacuum heat collection tubes are open. The open ends of the vacuum heat collection tubes are inserted into the connectors at the lower end of the air header. Flow-dividing stop plates are arranged in portions, close to the open ends, of the vacuum heat collection tubes. Flow-dividing cylinders sleeve the ends, inserted into the collectors, of the vacuum heat collection tubes. Each vacuum heat collection tube comprises at least one splicing tube and a main tube. The splicing tubes are collected with the main tubes through splicing components hermetically. The splicing tubes are connected with one another through splicing components hermetically. The air heat collector has the advantages that output hot air can be prevented from being mixed with cold air, the lengths of the vacuum heat collection tubes are controlled through splicing of the splicing tubes and the main tubes, and the application range is wide.
Owner:浙江潮城科技发展有限公司

A multiple fluorescence immunoassay method and reagents for rapidly distinguishing ilTV, ibv, mg and ms

The invention discloses a multiple fluoroimmunoassay method capable of quickly differentiating infectious laryngotracheitis virus (ILTV), infectious bronchitis virus (IBV), myeoplasma gallisepticum (MG) and mycoplasma synoviae (MS) and a reagent. The method is simple to operate, a target amplified fragment is obtained through polymerase chain reaction (PCR); an amplified product, fluorescence coded microspheres and streptavidin-phycoerythrin are hybridized, a mean fluorescence intensity (MFI) value is read through a detector, and pathogens of different types are differentiated. According to the method, avian infectious laryngotracheitis virus, infectious bronchitis virus, myeoplasma gallisepticum and mycoplasma synoviae can be accurately detected simultaneously, and the method is high in specificity and sensitivity and good in repeatability. Compared with a conventional detection method, the method can be used to simultaneously detect various target molecules in an identical sample, the sample usage amount is small, the method is simple and quick to operate, and the detection cost can be greatly reduced. The method is good in flexibility, and the varieties of detected pathogens can be increased or decreased as required on the basis.
Owner:GUANGDONG LAB ANIMALS MONITORING INST

A multiplex fluorescence analysis method and kit for simultaneously detecting 4 strains of rat parvoviruses

The invention discloses a multiplex fluorescence analysis method for simultaneous detection of four rat parvoviruses and a kit. The method is simple to operate. By PCR, a target amplified fragment is obtained; then, the amplified product, fluorescence coded microspheres and streptavidin-phycoerythrin are hybridized; and through a detector, MFI value is read to distinguish pathogenies of different types. The method can simultaneously detect and identify rat parvoviruses RMV, KRV, H-1 and PRV-1a, has advantages of strong specificity, high sensitivity, good repeatability and the like, and can realize simultaneous detection of various different target molecules in a same sample. The method has good flexibility. Types of detected pathogenies can be increased or reduced according to needs.
Owner:GUANGDONG LAB ANIMALS MONITORING INST

A Multiplex Fluorescence Immunoassay Method for Rapid Differentiation of 6 Avian Respiratory Pathogens

The invention discloses a multi-fluorescent immunoassay method for rapidly distinguishing 6 types of poultry respiratory pathogens. The multi-fluorescent immunoassay method is simple to operate; a target amplified fragment is obtained through a PCR (Polymerase Chain Reaction); then an amplified product, fluorescence coded microspheres and streptavidin-phycoerythrin are hybridized; an MFI (Mean Fluorescence Intensity) value is read through a detector to distinguish viruses of different types. According to the method disclosed by the invention, avian influenza viruses, chicken infectious bronchitis viruses, chicken Newcastle disease viruses, chicken infectious laryngotracheitis viruses, mycoplasma gallisepticum and mycoplasma synoviae can be accurately detected at the same time; the multi-fluorescent immunoassay method has high specificity, high sensitivity and good repeatability. Compared with a traditional detection method, the method disclosed by the invention realizes simultaneous detection of a plurality of types of different target molecules in the same sample; the use amount of the sample is less; the method is simple and rapid to operate and the detection cost can be greatly reduced.
Owner:GUANGDONG LAB ANIMALS MONITORING INST

A SNP site related to growth traits of Chlamys farreri and its application

The invention provides an SNP (single nucleotide polymorphism) site relevant to chlamys farreri growth traits. The site is the 846th bit of Smad gene with the nucleotide sequence being SEQ ID NO:2, and the basic group is G or T. The invention also provides a serotype specific primer and a probe for detecting the SNP site. A high-resolution melting curve technology is used for detecting the site polymorphism in a chlamys farreri group, and analyzing the relevance between the site genotype frequency and the farreri production traits. The site C.846G is greater than T and is significantly relevant to the important growth traits such as chlamys farreri weight, soft weight and closed shell muscle weight; the growth traits of GG type individuals are significantly higher than those of GT type individuals (P is smaller than 0.05).
Owner:OCEAN UNIV OF CHINA

A multiple fluorescence immunoassay method and reagents for rapidly distinguishing rabbit plague virus, Sendai virus and rabbit rotavirus

Disclosed are a multiplex fluoroimmunoassay method for rapidly distinguishing the rabbit hemorrhagic disease virus, Sendai virus and lapine rotavirus, and a reagent. The fluoroimmunoassay method comprises: obtaining a target amplification fragment by means of PCR; then hybridizing an amplification product, fluorescence coded microspheres and streptavidin-phycoerythrin; reading the MFI value through a detector; and distinguishing different types of viruses.
Owner:GUANGDONG LAB ANIMALS MONITORING INST

A kind of multiplex immunofluorescence analysis primer, kit and method for detecting chicken Marek's disease virus and chicken infectious anemia virus

The invention discloses multi-immunofluorescence assay primers, a kit and a method for detecting chicken Marek's disease viruses and chicken infectious anemia viruses. The primers, kit and method are simple in operation. The method comprises acquiring a target amplified fragment by PCR, then hybridizing the amplified product, fluorescence-coded microspheres and streptavidin-phycoerythrin, then reading an MFI value through a detector and distinguishing the different types of pathogens. The method can simultaneously detect the chicken Marek's disease viruses and chicken infectious anemia viruses, has the advantages of strong specificity, high sensitivity and good reproducibility, and can realize simultaneous detection of many different target molecules in the same sample. The multi-immunofluorescence assay primers have good flexibility and can detect more or less types of pathogens on the basis of the demands.
Owner:GUANGDONG LAB ANIMALS MONITORING INST

Bladder cancer detection kit

The invention provides a bladder cancer detection kit. The bladder cancer detection kit comprises at least one of detection probes for chromosomes 3, 7 and 17 and a gene p16; the detection probes forthe chromosomes 3, 7 and 17 and the gene p16 include a probe A, a probe B1, a probe B2, a probe C, a probe D and a probe E; a base of the probe A from the 5' end to the 3' end is composed of a P1 sequence, M1, a specific P2 sequence, M1 and a P1 sequence; the probe B1 is a P3 sequence; the probe B2 is a P4 sequence; a base of the probe C from the 5' end to the 3' end is composed of a P5 sequence,M2 and a P6 sequence; the probe D is a P7 sequence; and a base of the probe E from the 5' end to the 3' end is composed of a P8 sequence of which the 5' end is modified with a fluorophore and the 3' end is modified with a structure capable of quenching the fluorophore. The bladder cancer detection kit has the advantages of short hybridization time, high sensitivity, strong specificity, high accuracy, simple operation steps and the like.
Owner:SUREXAM BIO TECH

A multiple liquid phase gene chip method and reagents for rapid detection of guinea pig lcmv, sv, pvm, reo-3 viruses

The invention discloses a multiple liquid phase gene chip method and a reagent for rapidly detecting guinea pig LCMV, SV, PVM and Reo-3 viruses. The operation is simple. A target amplified fragment is acquired through PCR, and then the amplified product, fluorescent coding micro-balloon and streptavidin-phycoerythrin are hybridized, MFI value is read through a tester and different viruses are distinguished. According to the method provided by the invention, the rat lymphocyte choriomeningitis virus, sendai virus, rat pneumonia virus and Reo-3 virus can be accurately detected at the same time; the specificity is strong, the sensitivity is high and the repeatability is excellent; compared with the traditional detection method, the method has the advantages that different target molecules in a same sample can be detected, the detection flux is high, the sample dosage is less, the operation is simple and quick, the detection cost is greatly reduced and the detection efficiency is increased.
Owner:GUANGDONG LAB ANIMALS MONITORING INST
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