38results about How to "Avoid crossbreeding" patented technology

The invention provides an SNP locus related to growth characteristics of patinopecten yessoensis. The locus is the 1054 site of IGFBP (Insulin Like Growth Factor Binding Proteins) genes of which the nucleotide sequence is shown as SEQ ID NO:2 and has the base of A or G. The invention also provides a probe for detecting the SNP locus and a parting primer. The transcription sequence of the IGFBP genes in the patinopecten yessoensis is subjected to sequencing and Clustal comparison, and three SNP loci are screened. The site polymorphism is detected in the patinopecten yessoensis group by using a high resolution melting curve technology, and correlation between the site genotype frequency and the growth characteristics of patinopecten yessoensis is analyzed. The locus C.1054A>G is obviously related to important growth characteristics such as the height, shell length, weight, soft weight and adductor muscle weight of the patinopecten yessoensis, the growth characteristics of the AG type individuals are obviously lower than those of AA and GG type individuals (P is less than 0.05), and the characteristic value of the GG type is the highest. Therefore, individuals of which the genotype is GG on the locus can be preferentially selected during production and are used as parents for performing high-yield patinopecten yessoensis breeding or performing large-scale breeding.

The invention discloses a multiple liquid phase gene chip method and a reagent for rapidly detecting guinea pig LCMV, SV, PVM and Reo-3 viruses. The operation is simple. A target amplified fragment is acquired through PCR, and then the amplified product, fluorescent coding micro-balloon and streptavidin-phycoerythrin are hybridized, MFI value is read through a tester and different viruses are distinguished. According to the method provided by the invention, the rat lymphocyte choriomeningitis virus, sendai virus, rat pneumonia virus and Reo-3 virus can be accurately detected at the same time; the specificity is strong, the sensitivity is high and the repeatability is excellent; compared with the traditional detection method, the method has the advantages that different target molecules in a same sample can be detected, the detection flux is high, the sample dosage is less, the operation is simple and quick, the detection cost is greatly reduced and the detection efficiency is increased.

The invention discloses a multiple fluoroimmunoassay primer, kit and method for rapidly distinguishing 5 avian immunosuppression pathogens. Operation is simple, a target amplified fragment is acquired through a PCR, an amplified product, fluorescence coded microspheres and streptavidin-phycoerythrin are hybridized, the MFI value is read through a detector, and viruses of different types are distinguished. The method can simultaneously detect the chicken infectious anemia virus, the chicken Marek's disease virus, the avian reticuloendotheliosis virus, the avian reovirus and the chicken infectious bursa disease virus accurately, specificity is high, sensitivity is high, and repeatability is good. Compared with a traditional detection method, the method achieves simultaneous detection of multiple different target molecules in the same sample, the use number of samples is small, operation is simple and rapid, and the detection cost can be greatly reduced.

The invention discloses a multi-liquid-phase gene chip detection primer, kit and method for quickly distinguishing 5 respiratory pathogens of a mouse. The multi-liquid-phase gene chip detection primer is easy to operate; a target amplified fragment is obtained through PCR (Polymerase Chain Reaction); an amplified product, fluorescence coded microspheres and streptavidin-phycoerythrin are hybridized; an MFI (Mean Fluorescence Intensity) value is read through a detector so as to distinguish different types of viruses. The method disclosed by the invention can detect a pneumonia virus, a hantaan virus, a sendai virus, a lymphocytic choriomeningitis virus and mycoplasma pulmonis of the mouse at the same time, and is high in specificity, high in sensitivity and high in repetitiveness. Compared with the conventional detection method, the method disclosed by the invention realizes simultaneous detection of various different target molecules in the same sample; the sample use amount is small; the operation is simple and quick; the detection cost can be greatly reduced.

The invention discloses a multiplex fluorescence immunoassay primer, kit and method for rapidly distinguishing four respiratory pathogens. Operation is easy, a target amplified fragment is obtained through a PCR, then an amplified product, fluorescence coded microspheres and streptavidin-phycoerythrin are hybridized, an MFI value is read through a detector, and viruses of different types are distinguished. The method can be used for accurately detecting chicken new castle disease viruses, avian influenza viruses, chicken infectious bronchitis viruses and chicken infectious laryngotracheitis viruses at the same time, and is high in specificity, high in sensitivity and good in repeatability. Compared with a traditional detection method, the method can detect multiple different target molecules in the same sample at the same time, the use quantity of samples is small, operation is easy and rapid, and the detection cost can be greatly reduced. The flexibility is good, and the varieties of detected pathogens can be increased or decreased on the basis according to requirements.

The invention discloses multi-immunofluorescence assay primers, a kit and a method for detecting chicken Marek's disease viruses and chicken infectious anemia viruses. The primers, kit and method are simple in operation. The method comprises acquiring a target amplified fragment by PCR, then hybridizing the amplified product, fluorescence-coded microspheres and streptavidin-phycoerythrin, then reading an MFI value through a detector and distinguishing the different types of pathogens. The method can simultaneously detect the chicken Marek's disease viruses and chicken infectious anemia viruses, has the advantages of strong specificity, high sensitivity and good reproducibility, and can realize simultaneous detection of many different target molecules in the same sample. The multi-immunofluorescence assay primers have good flexibility and can detect more or less types of pathogens on the basis of the demands.

The invention discloses an amplification method for 3'-RACE adaptor primer and 3'-end unknown gene sequences. The 3'-RACE adaptor primer is of a stem-loop structure composed of a stem handle region, astem-loop region and a PolyA binding region; the stem handle region and the stem-loop region have a nucleotide sequence shown in SEQ ID NO.1; and 15, 16, 17, 18, 19 or 20 continuous thymine are designed in the PolyA binding region. Two fragments of the stem handle region of the 3'-RACE adaptor primer have complementary sequence base so as to form a handle of the stem loop. Due to base stacking atthe handle, the stability of the primer is increased, and the reverse transcription efficiency is improved. A stem-loop configuration is formed due to a double-chain structure and self-complementaryproperty, so that hybridization between a reverse transcription primer and a target RNA template can be avoided, and background interference is reduced. More G/C bases are particularly inserted into the stem-loop region of the 3'-RACE adaptor primer, so that the annealing temperature is increased, and the non-specific PCR amplification is reduced.

The invention provides a yarn impurity removing device in a warp guide device of a warp knitting machine, and belongs to the technical field of machines. The warp guide device of the warp knitting machine comprises a machine frame and a tank arranged on the machine frame, wherein a lubricating shaft is rotationally arranged in the tank. The yarn impurity removing device comprises an air pipe arranged above the tank, and the axial direction of the air pipe is consistent with the axial direction of the lubricating shaft; the two ends of the air pipe are a closed end and an opened end respectively, a plurality of exhaust holes are formed in the lower side of the air pipe in a penetrating mode, and the exhaust holes are aligned with the lubrication shaft; a fan is further fixedly arranged on the machine frame, the fan comprises a straight tubular air outlet part, and a stainless steel corrugated pipe making the opened end and the air outlet part communicated is arranged between the openedend and the air outlet part; an adjusting pipe and a connecting column are vertically arranged on the lower portion of the closed end, and the lower end of the adjusting pipe is fixedly connected withthe machine frame; the upper end of the connecting column is fixedly connected with the closed end, the lower end of the connecting column is inserted into the adjusting pipe, and a detachable locking mechanism for fixing the connecting column and the adjusting pipe together is arranged between the connecting column and the adjusting pipe. The yarn impurity removing device has the advantage thatyarn crossing can be prevented.

The invention aims to provide a breeding method of an eggplant. The eggplant variety is prepared by purifying and stabilizing an eggplant variety which is mutated after being carried by a spacecraft, and has the advantages of high disease resistance, high yield and the like.