6 results about "Hantaan virus" patented technology

The invention belongs to the technical field of construction methods of disease animal models, and particularly relates to a construction method and application of a renal syndrome hemorrhagic fever mouse model. According to the HTNV infected mouse model established by the method disclosed by the invention, the symptom of the experimental mouse is close to the clinical symptom of a severe hemorrhagic fever with renal syndrome after challenge, the complete lethality and repeatability are high, and the HTNV infected mouse model has the advantages of simple operation, low cost and high efficiency. The method can be used for research on the HTNV pathogenic mechanism and immune mechanism and research and development of antiviral drugs and vaccines, and has great application value for perfecting an HTNV full-chain prevention and control system.

This invention provides the application of apatinib in the preparation of antiviral drugs, specifically involving the application of the anticancer drug apatinib in inhibiting the replication of Hantanensis virus (HTNV) and Chikungunya virus (CHIKV). Apatinib can significantly inhibit HTNV replication and reduce viral titers at the cellular level. Experiments have shown that after treatment with apatinib, the expression levels of viral structural proteins, the nucleic acid levels of HTNV, and the viral titers in the cell culture supernatant were significantly reduced in HTNV-infected A549 cells, while the drug exhibited low cytotoxicity. Furthermore, apatinib can also inhibit CHIKV replication. This invention provides a novel anti-HTNV candidate drug and offers insights for the rapid development of antiviral drugs.

The application relates to the field of biological medicine, and particularly discloses application of celadixyn in preparation of an anti-Hantaan virus drug, wherein the celadixyn realizes anti-Hantaan virus by inhibiting replication and proliferation of the Hantaan virus. Experiments prove that the celadixyn directly targets HTNV NP to play an anti-virus effect. The chemical structural formula of the celadixyn is as follows:

The present disclosure is directed to antibodies binding to and neutralizing Hantaan Virus and methods for use thereof.

This invention belongs to the field of virus-infected cell line technology, and particularly relates to a striped field mouse lung cell line and its applications. The striped field mouse lung cell line has the accession number CCTCC NO: C2025268. The AaL-09W cell line (including primary and passaged cells) provided by this invention has multiple advantages, including high sensitivity to Hantavirus, high replication efficiency, high isolation success rate, closer resemblance to the host's natural environment, passage stability, and easy scaling-up. It provides a reliable cellular tool and an efficient, reliable, and scalable experimental platform for the isolation, identification, in vitro culture, titer determination, infection mechanism and pathogenesis research, new drug screening, and vaccine development of Hantavirus, meeting the needs of efficient culture and multi-scenario applications of HTNV and related Hantaviruses.

The invention relates to a CRISPR / Cas13 (clustered regularly interspaced short palindromic repeats / CRISPR associated protein 13) system and application of the CRISPR / Cas13 system in preparation of anti-Hantaan virus drugs. The CRISPR / Cas13 system comprises Wherein the Cas13 protein is a CasRx protein, the crRNA comprises crRNA1, crRNA2, crRNA6 and crRNA7, and the sequences of the crRNA1, the crRNA2, the crRNA6 and the crRNA7 are respectively SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 6 and SEQ ID NO: 7. The crRNA in targeted complementary pairing with the virus RNA and messenger RNA corresponding to the Hantaan virus S gene is designed, the cleavage activity of CasRx protein can be guided and activated, then specific cleavage is conducted on the virus RNA or messenger RNA, the purpose of inhibiting hantaan virus replication is achieved, a new thought and method are provided for treatment of Hantaan virus infection, and the method has a wide application prospect. The important clinical application value is realized.