9 results about "Hantaan virus" patented technology

The invention belongs to the technical field of construction methods of disease animal models, and particularly relates to a construction method and application of a renal syndrome hemorrhagic fever mouse model. According to the HTNV infected mouse model established by the method disclosed by the invention, the symptom of the experimental mouse is close to the clinical symptom of a severe hemorrhagic fever with renal syndrome after challenge, the complete lethality and repeatability are high, and the HTNV infected mouse model has the advantages of simple operation, low cost and high efficiency. The method can be used for research on the HTNV pathogenic mechanism and immune mechanism and research and development of antiviral drugs and vaccines, and has great application value for perfecting an HTNV full-chain prevention and control system.

This invention provides the application of apatinib in the preparation of antiviral drugs, specifically involving the application of the anticancer drug apatinib in inhibiting the replication of Hantanensis virus (HTNV) and Chikungunya virus (CHIKV). Apatinib can significantly inhibit HTNV replication and reduce viral titers at the cellular level. Experiments have shown that after treatment with apatinib, the expression levels of viral structural proteins, the nucleic acid levels of HTNV, and the viral titers in the cell culture supernatant were significantly reduced in HTNV-infected A549 cells, while the drug exhibited low cytotoxicity. Furthermore, apatinib can also inhibit CHIKV replication. This invention provides a novel anti-HTNV candidate drug and offers insights for the rapid development of antiviral drugs.

The application relates to the field of biological medicine, and particularly discloses application of celadixyn in preparation of an anti-Hantaan virus drug, wherein the celadixyn realizes anti-Hantaan virus by inhibiting replication and proliferation of the Hantaan virus. Experiments prove that the celadixyn directly targets HTNV NP to play an anti-virus effect. The chemical structural formula of the celadixyn is as follows:

The present disclosure is directed to antibodies binding to and neutralizing Hantaan Virus and methods for use thereof.

The present invention belongs to the technical field of antiviral drugs, specifically to the application of emodin in the preparation of drugs against Hantaan virus. The concentration of emodin against Hantaan virus in vitro is 25 - 100 μM, and the concentration of emodin against Hantaan virus in vivo is 25 - 100 mg / kg. It is found that emodin can inhibit the transcription and translation of HTNV virus and can be used to prepare products against Hantaan virus.

The invention discloses a crRNA target spot and a CRISPR-Cas13a system for detecting hantaan virus and application of the crRNA target spot and the CRISPR-Cas13a system. The CRISPR-Cas13a system comprises Cas13a protein and crRNA or a complex formed by the Cas13a protein and the crRNA; the crRNA comprises an anchoring sequence used for being combined with Cas13a protein and a guide sequence targeting a Hantaan virus target sequence; the Hantaan virus target sequence is located at the 2642-2973 site of the L-segment gene of the Hantaan virus genome. The crRNA provided by the invention can realize high-sensitivity and high-specificity detection on Hantaan virus nucleic acid by activating Cas13a, and the sensitivity reaches 1 copy (1 copy / [mu] L).

The invention relates to the technical field of biological detection, and provides a primer probe set for combined detection of multiple rodent-derived viruses and a digital droplet PCR detection kit. The primer probe set comprises a hantaan virus detection PCR primer probe, a first virus detection PCR primer probe, a new bunyavirus detection PCR primer probe and an internal standard GAPDH gene primer probe, and the sequences are shown as SEQ ID NO.1-12. The digital droplet PCR detection kit comprises the primer probe set provided by the invention. According to the primer probe set and the digital droplet PCR detection kit disclosed by the invention, multiple nucleic acid targets can be effectively and jointly detected and accurately quantified; the kit has the advantages of high throughput, capability of realizing accurate quantification of low-abundance nucleic acid, high detection sensitivity, good repeatability, strong specificity, simplicity and convenience in operation and capability of providing an accurate detection result within 2-3 hours.

This invention belongs to the field of virus-infected cell line technology, and particularly relates to a striped field mouse lung cell line and its applications. The striped field mouse lung cell line has the accession number CCTCC NO: C2025268. The AaL-09W cell line (including primary and passaged cells) provided by this invention has multiple advantages, including high sensitivity to Hantavirus, high replication efficiency, high isolation success rate, closer resemblance to the host's natural environment, passage stability, and easy scaling-up. It provides a reliable cellular tool and an efficient, reliable, and scalable experimental platform for the isolation, identification, in vitro culture, titer determination, infection mechanism and pathogenesis research, new drug screening, and vaccine development of Hantavirus, meeting the needs of efficient culture and multi-scenario applications of HTNV and related Hantaviruses.

The invention relates to a CRISPR / Cas13 (clustered regularly interspaced short palindromic repeats / CRISPR associated protein 13) system and application of the CRISPR / Cas13 system in preparation of anti-Hantaan virus drugs. The CRISPR / Cas13 system comprises Wherein the Cas13 protein is a CasRx protein, the crRNA comprises crRNA1, crRNA2, crRNA6 and crRNA7, and the sequences of the crRNA1, the crRNA2, the crRNA6 and the crRNA7 are respectively SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 6 and SEQ ID NO: 7. The crRNA in targeted complementary pairing with the virus RNA and messenger RNA corresponding to the Hantaan virus S gene is designed, the cleavage activity of CasRx protein can be guided and activated, then specific cleavage is conducted on the virus RNA or messenger RNA, the purpose of inhibiting hantaan virus replication is achieved, a new thought and method are provided for treatment of Hantaan virus infection, and the method has a wide application prospect. The important clinical application value is realized.