Double T-DNA vector capable of achieving agrobacterium co-transformation and establishment method and application thereof

A technology of co-transformation and Agrobacterium, applied in the field of genetic engineering, can solve the problems of inability to obtain transgenic positive plants, wrong retention, and difficulty in achieving separation, and achieve the effect of simple and rapid elimination.
CN105505988AInactive Publication Date: 2016-04-20SICHUAN AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SICHUAN AGRI UNIV
Publication Date
2016-04-20
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a double T-DNA vector capable of achieving agrobacterium co-transformation. The double T-DNA vector is obtained by serially connecting a reporter gene GUS and a resistant selection marker gene, serially connecting a fluorescence reporter gene and a target gene, then respectively connecting the two serially connected sequences to two independent T-DNA areas and establishing the sequences to a plant binary expression vector. The vector is utilized to cultivate transgenic plants without selection markers and can quickly test positive transgenic plants from offspring.
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Description

technical field

[0001] The invention belongs to the field of genetic engineering, and in particular relates to a double T-DNA carrier capable of realizing the co-transformation of Agrobacterium and its construction method and application. Background technique

[0002] In today's China, the contradiction between food supply and demand is becoming increasingly prominent. As the most direct and effective technical means to improve crop varieties and improve crop yield and quality, genetic engineering technology has more and more important practical significance.

[0003] Transgenic technology emerged in the 1980s. At present, this technology has become the most direct and effective technical method for high-yield, high-quality, high-resistance, and wide-ranging crop breeding. This technology has been successfully applied to the improvement of economic crops, food crops, vegetables, flowers, medicinal plants, fruit trees and pastures. The data report pointed out that in 2014, ...

Claims

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