4 results about "Mean fluorescence intensity" patented technology

This invention relates to a rapid screening method for bone-repairing surface structures, belonging to the field of bone repair materials technology. It aims to solve the technical problems of low efficiency and low individual / group adaptability of traditional screening methods. This invention constructs a titanium dioxide nanotube array with a continuous gradient diameter of 0-200 nm using a four-electrode bipolar electrochemical method. Immunofluorescence staining of key bone repair factors BMP-2, RUNX2, OPN, and OCN, combined with panoramic scanning analysis, is used to normalize the average fluorescence intensity of each of the four factors and then sum them to obtain a comprehensive osteogenic coefficient. Based on this coefficient, the optimal bone-repairing surface structure corresponding to bone marrow mesenchymal stem cells (BMSCs) from different age groups or different pathological conditions (such as osteoporosis, osteoarthritis, etc.) can be precisely located. This invention achieves high-throughput screening covering multiple surface structures in a single experiment, providing an efficient technical path for the precise and personalized design of bone repair materials, and has significant clinical translational value.

This invention discloses a method for electroporation culture of dendritic cells (DCs), comprising the following steps: On day 0, PBMC suspension is seeded into a T175 culture flask, and a first culture medium (including basal medium, rhGM-CSF, IL-4, and VC components) is added and the flask is placed in an incubator for culture; On day 3, the first culture medium is added again and culture continues; On day 5, the culture medium in the culture flask is aspirated, and a second culture medium (including basal medium, rhGM-CSF, IL-4, IL-6, TNF-α, IL-1β, and VC) is added; On day 7, the cells are transferred to an electroporation cuvette, electroporation buffer is added, electroporation is performed, and then the second culture medium is added again and culture continues; On day 10, culture is stopped, the cells are collected, centrifuged, and the supernatant is removed to obtain DCs. Electroporation followed by culture significantly increases the viability of mature DCs, reaching approximately 90%; the mean fluorescence intensity (MFI) of mature DCs is significantly increased (up to 10⁴²¹), and the antigen expression level of DCs is good.

This invention relates to the field of biomedical technology, and provides CD177. + Application of granulocyte subsets in the preparation of kits for the auxiliary diagnosis of tuberculosis. The CD177... + Granulocyte subsets include CD177 + Neutrophils and CD177 + Low-density granulocytes; the kit detects CD177. + The proportion of neutrophil-positive cells and CD177 + The mean fluorescence intensity of low-density granulocytes aids in the diagnosis of tuberculosis. This invention utilizes CD177. + Granulocyte subsets, as biomarkers, play an important role in auxiliary diagnosis, disease activity monitoring, and improving diagnostic efficiency. Based on CD177... + The proportion of neutrophil-positive cells, CD177 + By using the mean fluorescence intensity of low-density granulocytes, the ratio of neutrophils to lymphocytes, the ratio of platelets to lymphocytes, and the systemic inflammatory index, a predictive model for the auxiliary diagnosis of tuberculosis can be constructed. This model can more accurately identify true tuberculosis patients and effectively exclude non-patients, reducing the risk of misdiagnosis and missed diagnosis.

The application discloses a method for high-throughput screening of compounds promoting degradation of mitochondria of inflammatory microglia cells, and belongs to the technical field of biological medicine screening. The method comprises the following steps: stimulating microglia cells to establish a mitochondrial accumulation model by using an inflammation inducer; adding a candidate drug and the inflammation inducer into the cells for incubation; adding a mitochondria-specific fluorescent probe for dark staining; detecting the average fluorescence intensity of the cells by using flow cytometry; and taking the fluorescence intensity of a positive control group as a baseline, if the average fluorescence intensity of a candidate drug treatment group is significantly reduced, it is indicated that the compound has the activity of promoting degradation of mitochondria of inflammatory microglia cells. The method can realize high-throughput, rapid and objective quantitative screening, is suitable for large-scale drug screening, and provides an effective tool for developing drugs for relieving neural inflammation.