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Method for quantitatively detecting protein concentration by flow cytometer

A protein concentration and flow cytometry technology, applied in the field of quantitative detection of protein concentration by flow cytometer, can solve the problems of complicated operation steps, and achieve the effects of convenient and fast operation, shortening reaction time and small reaction system.

Active Publication Date: 2016-06-29
王博
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AI Technical Summary

Problems solved by technology

This method has the advantages of being fast, sensitive, simple and the carrier is easy to standardize, but the specific operation steps are cumbersome, and each reaction system can only detect one indicator, and the lower limit of detection of this method is at the ng / ml level

Method used

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  • Method for quantitatively detecting protein concentration by flow cytometer
  • Method for quantitatively detecting protein concentration by flow cytometer
  • Method for quantitatively detecting protein concentration by flow cytometer

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Embodiment Construction

[0023] In order to better understand and implement the present invention, the present invention will be further described in detail below in conjunction with specific implementation examples.

[0024] A method for quantitatively detecting protein concentration by flow cytometry, combining fluorescent immunomagnetic microspheres and flow cytometry to detect specific protein concentration, selecting rhodamine 123-labeled magnetic microspheres, and PE-cy7-labeled detection of antibodies, the specific method is as follows:

[0025] 1. Preparation of fluorescent magnetic microspheres: Prepare fluorescent magnetic microspheres by modifying fluorescent dyes on the magnetic microspheres. The specific preparation method is as follows:

[0026] (1) Weigh 3 mg of rhodamine 123 (Rh123) dye, dissolve it in 10 mL of deionized water to prepare a 1 mmol / L dye mother liquor. Rhodamine 123 or PE-cy7 is used as an example here, and any two fluorescent dyes in different channels of the flow cytom...

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Abstract

The invention discloses a method for quantitatively detecting protein concentration by a flow cytometer, and specific protein concentration is detected by the combination of fluorescence immunological magnetic microspheres and flow cytometric detection. The method comprises the specific steps: preparing a fluorescence detection antibody; preparing fluorescence immunological magnetic microspheres and calibrating magnetic microspheres; making a standard article working curve, and acquiring actual protein concentration through unknown sample average fluorescence strength. The flow cytometry and the fluorescence immunological magnetic microspheres are combined for detecting protein concentration; specific protein molecular concentration is detected by using the method, a reaction system is small, measuring is more accurate, a detection lower limit is pg / ml level, specificity and sensitivity are high, operating is more convenient and quick, operating steps are simpler, incubation is direct without stepwise incubation and washing so that reaction time is shortened, detection results are obtained within a shorter time, and quantitating is more accurate.

Description

technical field [0001] The invention relates to a method for quantitatively detecting protein concentration, in particular to a method for quantitatively detecting protein concentration with a flow cytometer. Background technique [0002] Biomacromolecules are multi-molecular systems formed by the polymerization of organic compounds with simple structures and relatively low molecular weights, including proteins, nucleic acids, lipids, and sugars. Biological macromolecules have various biological activities and play an important role in biological metabolism, so they are the basic substances that constitute life. Proteins, in particular, are the basic functional units that make up human tissues and are the main bearers of life activities. At the same time, they perform biological functions through complex structural characteristics, such as providing energy and substances needed by life, promoting growth and development, and producing immune functions. [0003] The research ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/14G01N33/68
CPCG01N15/1468G01N33/68
Inventor 王博
Owner 王博
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