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Biological method for detecting snail meat ingredients

A technology of biology and snail meat, applied in the field of biology to detect the components of snail meat, can solve the problems of harming the health and affecting the taste of snail powder, and achieve the effects of less medicine, saving detection time and improving accuracy

Inactive Publication Date: 2018-11-02
LUSHAN COLLEGE OF GUANGXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, many unscrupulous merchants do not put snail meat in the soup at all in order to save costs. What’s more, they directly use chemical seasonings to make “snail noodle soup”, which not only affects the taste of snail noodle, but also directly damages the taste of the noodle. people's health

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035]A biological method for detecting snail meat components, the method comprising the following steps:

[0036] ①Take snail noodle soup as a sample, remove impurities from the sample, and extract DNA from snail meat with a DNA kit; digest the total DNA and recover the digested product;

[0037] ②Design primers according to the exon of snail mitochondrial cytochrome C oxidase subunit I gene (cox1), the primers are: Foward primerTTCTAGTTGATTGCCTGCG; Reverse primerTAACTAGTCCAACACCAGA;

[0038] ③Using the digested product as a template, set a positive control and a blank control at the same time, add the designed primers respectively, and perform PCR amplification; the PCR amplification system is 40 μl; the DNA template is 1 μl; the primer concentration is 1 μmol / L, and the forward and reverse primers are each 1μl; 4 kinds of dNTP concentrations of 50umol / L, 1μl each; pH value of 8.2-8.3 buffer Tris-HCL, 5μl; KCl 50mmol / L, 1μl; MgCl 2 1.5mmol / L, 1μl; Tag DNase 2U; ddH 2 O 15 ...

Embodiment 2

[0050] A biological method for detecting snail meat components, the method comprising the following steps:

[0051] ①Take snail noodle soup as a sample, remove impurities from the sample, and extract DNA from snail meat with a DNA kit; digest the total DNA and recover the digested product;

[0052] ②Design primers according to the exon of snail mitochondrial cytochrome C oxidase subunit I gene (cox1), the primers are: Foward primerTTCTAGTTGATTGCCTGCG; Reverse primerTAACTAGTCCAACACCAGA;

[0053] ③ Use the digested product as a template, set a positive control and a blank control at the same time, add the designed primers respectively, and carry out PCR amplification; the PCR amplification system is 50 μl; the DNA template is 2 μl; the primer concentration is 1.5umol / L, and the forward and reverse primers 1 μl each; 4 kinds of dNTP concentrations of 50umol / L, 1μl each; buffer solution Tris-HCL with a pH value of 8.2-8.3, 5μl; KCl 50mmol / L, 1μl; MgCl 2 1.5mmol / L, 1μl; Tag DNase ...

Embodiment 3

[0065] A biological method for detecting snail meat components, the method comprising the following steps:

[0066] ①Take snail noodle soup as a sample, remove impurities from the sample, and extract DNA from snail meat with a DNA kit; digest the total DNA and recover the digested product;

[0067] ②Design primers according to the exon of snail mitochondrial cytochrome C oxidase subunit I gene (cox1), the primers are: Foward primerTTCTAGTTGATTGCCTGCG; Reverse primerTAACTAGTCCAACACCAGA;

[0068] ③ Use the digested product as a template, set a positive control and a blank control at the same time, add the designed primers respectively, and carry out PCR amplification; the PCR amplification system is 60 μl; the DNA template is 3 μl; the primer concentration is 2umol / L, and the forward and reverse primers are respectively 1μl; 4 kinds of dNTP concentrations of 50umol / L, 1μl each; pH value of 8.2-8.3 buffer Tris-HCL, 5μl; KCl 50mmol / L, 1μl; MgCl 2 1.5mmol / L, 1μl; Tag DNase 2U; ddH...

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Abstract

The invention discloses a biological method for detecting snail meat ingredients and relates to a biological detection method. The method comprises steps as follows: (1) snail rice noodle soup is taken as a sample, impurity removal is performed on the sample, and DNA of the snail meat is extracted with a DNA kit; total DNA is subjected to enzyme digestion, and an enzyme digestion product is recovered; (2) primers are designed according to exons of mitochondrion cytochrome C oxidase subunit I genes of snails; (3) the enzyme digestion product is taken as a template, besides, positive control andblank control are set, and the designed primers are added respectively for PCR amplification; (4) an amplification product is precipitated and purified; (5) the amplification product is detected in agarose gel electrophoresis, the lengths of the positive control and the amplification product of the sample meet the expectation, negative control has no expected products, and that is, it can be determined that the snail meat ingredients exist in the sample. Whether the snail rice noodle soup contains snail meat can be detected, the detection speed is high, the accurate rate is high, and the method is simple and convenient and easy to popularize and use.

Description

technical field [0001] The invention relates to a biological detection method, in particular to a biological method for detecting snail meat components. Background technique [0002] Snail powder is a unique local snack rice noodle, which has a unique flavor of spicy, cool, fresh, sour and hot. The deliciousness of snail powder is also due to its unique soup. This soup is made of snail meat, kaempferen, star anise, cinnamon, cloves, peppers, etc., especially snail meat, which can greatly increase the delicious taste of snail powder soup. However, many unscrupulous merchants do not put snail meat in the soup at all in order to save costs. What’s more, they directly use chemical seasonings to make “snail noodle soup”, which not only affects the taste of snail noodle, but also directly damages the taste of the noodle. people's health. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a biological method for detectin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888
CPCC12Q1/6888
Inventor 胡江如熊建文唐晓雷胡艺林李璐凡王春婷汪雪
Owner LUSHAN COLLEGE OF GUANGXI UNIV OF SCI & TECH
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