Method and system for automatically recognizing rare cells

A rare cell and automatic identification technology, applied in the field of biomedical inspection, can solve the problems of too large smear area, weak expression of CTC-specific antibodies, and difficulty in manually finding CTCs, so as to improve the effect of staining and identification
CN106190945AInactive Publication Date: 2016-12-07SHENZHEN HUADA GENE INST
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHENZHEN HUADA GENE INST
Publication Date
2016-12-07
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention relates to a method and a system for automatically recognizing rare cells. The method comprises: injecting a cell enrichment liquid carrying rare cells into a prepared coating-slice device, staining the cells according to a standard immunostaining process, and carrying out multi-fluorescence channel shooting through a fluorescence microscope to obtain a fluorescence image; treating the fluorescence image into a clean image strictly having a background gray value of zero and retaining the true fluorescence value in the cell contour, and carrying out cell contour extraction on the clean image; and carrying out statistics on the average fluorescence intensity value of various extracted contour channels corresponding to the multi-fluorescence channel, and determining the rare cells according to the statistics results. According to the present invention, the obtained fluorescence image is treated to strictly achieve the background gray value of zero while the true fluorescence value in the cell contour is retained, such that the influence of the background noise on the cell contour extraction is minimized; and for the fluorescence image obtained through the multi-fluorescence channel, the cells are confirmed and recognized from more parameters so as to improve the staining recognizing of the rare cells.
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Description

technical field

[0001] The invention relates to the technical field of biomedical testing, in particular to a method and system for automatically identifying rare cells. Background technique

[0002] As one of the most commonly used tools for identifying specific cells, immunofluorescence staining has a convenient operation process and direct effect, and has a wide range of applications in cell identification, medical diagnosis, and antibody expression detection. Laser capture microdissection (LCM) counting based on smear staining and recognition, compared with flow cytometry single-cell separation, has less damage to cells and is more conducive to molecular biology research of tumors. When the number of cells on the smear is small, it is easy to find the target cells with the naked eye under the fluorescence microscope. However, when the number of cells is large (more than 10 5 ), and when the number of target cells is relatively rare, it becomes extremely difficult to ma...

Claims

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