19 results about "Cell enrichment" patented technology

The present invention relates to a method and platform for generating CAR-T cells and processing biological cells within a single functionally closed container. The method involves introducing host cells into a sealed container with multiple sterile channels, performing genetic modifications, and maintaining the cells under controlled conditions monitored by real-time feedback until a desired fraction of genetically modified cells is obtained. The container also supports target cell separation using antibody or aptamer functionalized buoyant microbubbles, facilitating the separation of target cells from non-target cells during centrifugation. Waste separation and cell harvesting are performed using pneumatic pressure, enabling high purity cell recovery. The platform includes modules for cell enrichment, sequestration, and isolation, enabling high recovery and high purity of therapeutic cell populations. This integrated closed system approach ensures sterile conditions, efficient processing, and high quality cell products for advanced therapeutic applications.

The invention provides a brain glioma immune microenvironment complexity assessment method and application, and the method comprises the following steps: obtaining m glioma samples, and carrying out transcriptome determination on each cell in each glioma sample to obtain transcriptional expression profile data of the cells; carrying out clustering analysis on transcriptional expression profile data of the single cells through K-means clustering, and preliminarily identifying lymphocytes and myeloid cells according to immune cell classical markers; carrying out clustering analysis on the identified transcription expression profile data of the lymphocytes and myeloid cells, and identifying n cell subgroups; calculating the enrichment score of the characteristic gene set of each cell subset in the glioma transcriptome sample, wherein the higher the score is, the higher the enrichment proportion of the cell subset in the sample is; and evaluating the complexity of immune cell enrichment in the glioma sample based on the enrichment score of the characteristic gene set. The immune cell infiltration complexity can be predicted, and the relationship between immune cell enrichment complexity and brain glioma prognosis can be identified.

This invention provides a slide and a method for enriching cells by circulating centrifugation. The structure includes: an outer cylinder; a top cover disposed on the upper part of the outer cylinder; an inner cylinder disposed on the inner side of the outer cylinder; a receiving cavity formed between the outer wall of the inner cylinder and the inner wall of the outer cylinder; a slide housed in the lower part of the inner cavity; a valve component formed by the valve component on the bottom wall of the inner cylinder; a first downward inflow hole and a first upward outflow hole, with at least one first downward inflow hole provided in the lower part of the inner cylinder; and at least one first upward outflow hole provided in the upper part of the inner cylinder; the inner cavity of the inner cylinder is connected to the receiving cavity through the first downward inflow hole and the first upward outflow hole. This invention, through its infinite circulation function, can greatly improve the cell enrichment rate. For bodily fluids such as urine and transudate, which have large volumes and very few cells, it can collect as many cells as possible onto a single slide, thereby improving the diagnostic rate.

The present application relates to the technical field of biological cell collection and preservation, in particular to a peripheral blood immune cell efficient collection and low-temperature storage method, comprising: collecting peripheral blood samples and obtaining donor health status information, generating a sample cell composition atlas containing lymphocyte subpopulation absolute count percentage, monocyte purity and granulocyte contamination ratio through full-automatic blood cell analysis, determining the target immune cell optimal collection amount and adaptive collection process through a preset data analysis model, enriching target cells through gradient density centrifugation and specific magnetic bead sorting, comparing and judging supplementary collection by combining cell quality inspection data and expected collection amount, and then calculating individualized cryopreservation liquid formula, cooling program and storage strategy through a low-temperature storage optimization algorithm. The method can realize precise individualized adaptation of the collection process and storage parameters, and improve the cell enrichment purity and the cell state stability after low-temperature storage.

The invention belongs to the technical field of drug sensitivity detection, and particularly relates to a method for increasing the drug sensitivity detection speed of saccharomycetes and application of the method. The method comprises the steps of bacterial liquid preparation, sample adding, incubation, thallus enrichment, color developing agent adding and result interpretation, ethylenediamine tetraacetic acid or salt thereof needs to be added before thallus enrichment, and drug action and color developing are carried out separately. The detection method provided by the invention can effectively accelerate the developing speed of resazurin, remarkably shortens the drug sensitivity detection time, and is beneficial to early accurate diagnosis and treatment of clinical fungal diseases.

The utility model relates to cell culture bag technical field discloses a cell culture bag, including the bag body, the one side of bag body is provided with a plurality of connecting heads, a plurality of connecting head one side is connected with liquid inlet pipe subassembly, negative pressure needleless connector and cell enrichment and liquid supplementing device, cell enrichment and liquid supplementing device include the connecting pipe of setting up in corresponding connecting head one side, through setting up liquid inlet pipe subassembly, negative pressure needleless connector and cell enrichment and liquid supplementing device in the bag body one side, realized the integration function of liquid inlet, negative pressure operation, cell enrichment and liquid supplementing in cell culture process, the valve component design of cell enrichment and liquid supplementing device, can accurate control cell enrichment and culture medium supplement's process, avoid the interference caused by both simultaneously, guarantee the purity when cell enrichment and the sterile environment when liquid supplementing, thereby improve cell subculture expansion culture's efficiency and stability, reduce the operation step, reduce the pollution risk.

To provide cassettes for use in automated cell engineering systems that include cell concentration filters for reducing fluid volume of a cell sample during or following automated processing, and automated cell engineering systems.SOLUTION: A cassette comprises: (a) a cell culture chamber; (b) a pump fluidly connected to the cell culture chamber; (c) a tangential flow filter fluidly connected to the pump, wherein the pump provides a retentate flow to the tangential flow filter, wherein a permeate flow of the tangential flow filter is controlled by a flow controller; (d) a satellite volume connected to the tangential flow filter, contained in the cassette; (e) a fluid pathway for recirculating the retentate flow back through the tangential flow filter; (f) a fixed volume waste collection chamber fluidly connected to the tangential flow filter; and (g) a cellular sample output fluidly connected to the tangential flow filter.SELECTED DRAWING: Figure 1

The invention relates to an enrichment method of anaerobic nitrate reduction microorganisms and application thereof, and belongs to the technical field of microorganisms. According to the enrichment method disclosed by the invention, active cells in a solid sample are extracted by utilizing a Nycodenz density gradient centrifugation method, and then the active cells are inoculated into an anaerobic nitrate culture medium to carry out directional enrichment on anaerobic nitrate reducing microorganisms. According to the method, active cells can be efficiently enriched, the influence of a matrix is effectively reduced, and meanwhile, compared with a conventional cell enrichment technology, the efficiency is higher, and the cost is lower.

The application discloses a centrifugal tube and assembly for making cell wax blocks. The centrifugal tube comprises a ball-sac-equipped inner thread top cover, a centrifugal tube body composed of a funnel part and a cell enrichment tube, a cell collection capsule, a bottom pad and a bottom-sealed inner thread cover. The cell collection capsule and the bottom pad are arranged inside the cell enrichment tube to collect cells and can be taken in and out of the lower opening of the cell enrichment tube. After installation, detection liquid is added to the centrifugal tube body, and the centrifugal tube body is centrifuged by a centrifugal machine. The cells in the liquid are settled in the collection capsule. The bottom thread cover is unscrewed, the ball sac is pressed, and the cell collection capsule with the internal cell sediment is discharged from the lower opening of the cell enrichment tube under the hydraulic action. Then, the cell collection capsule is taken with the capsule cap and is placed in 10% neutral formalin solution for fixation. The fixing solution gradually penetrates into the cell mass, and the cell sediment gradually forms a certain shape and hardness. In this way, the cell wax block is simple and convenient to make and is not limited by the small amount of cells.

Disclosed herein are methods for single-cell sequencing. In some examples, the methods include enriching a sample comprising a plurality of cells for cells of interest to produce an enriched cell sample; isolating one or more cells of interest in the enriched cell sample; and obtaining sequence information of one or more polynucleotides from each of the one or more isolated cells. Obtaining sequence information may include generating a molecularly indexed polynucleotide library from the one or more isolated cells. Enriching the sample may include focusing cells of interest in the sample using acoustic focusing.

The invention discloses a high-throughput cell migration chip as well as a preparation method and an application method thereof. The high-throughput cell migration chip comprises a plurality of cell screening units, at least three injection ports, at least three migration channel outlets and a plurality of dampers, each cell screening unit comprises at least two cell culture chambers, at least two cell migration subgroup screening barriers and a cell enrichment chamber, and the cell enrichment chamber is separated from the cell culture chambers through the cell migration subgroup screening barriers; wherein the injection ports located on the two sides and the outlet of the migration channel are in fluid communication with the cell culture chamber, and the injection port located in the middle and the outlet of the migration channel are in fluid communication with the cell enrichment chamber; the damper is arranged in a flow channel system of the high-throughput cell migration chip, and the flow channel system is configured as follows: a flow channel structure comprising the damper is subjected to equal flow resistance design, so that a theoretical value of fluid pressure difference on two sides of a cell migration subgroup screening barrier is zero during fluid perfusion.

The present application relates to a kind of bionic lymph node organoids and its construction method, the organoids include in three-dimensional space: one B cell-rich core region;And one T cell-rich region, it is at least partially wrapped in the core region in space;Wherein, the core region can be differentiated to form the light area and dark area with structural difference in response to external stimulation.The method includes preparing first bioink and second bioink, the first bioink contains B lymphocyte, and the second bioink contains T lymphocyte;Using the first and second bioink, the organoid prototype with the spatial structure of T cell-rich region wrapping B cell-rich region is formed by three-dimensional construction technology;The organoid prototype is solidified to stabilize its structure;External stimulation is applied to the structure after solidification, and the B cell-rich region is differentiated to form light area and dark area.

The utility model discloses a multifunctional centrifugal tube, including pipe cover, tube body and pipe tail. The pipe cover is installed on the tube body, the pipe tail is detachably installed below the tube body, the pipe tail includes cell enrichment spare and pipe bottom spare, a plurality of hollow collection cavities are arranged on the cell enrichment spare, the collection cavity is connected with the tube body, the pipe bottom spare is detachably installed below the cell enrichment spare to block the bottom of the collection cavity, the pipe tail is divided into the upper and lower two parts of cell enrichment spare and pipe bottom spare, the connecting place of cell enrichment spare and pipe bottom spare is sealed through the tearable annular connecting band, and the connecting place of cell enrichment spare and tube body is sealed through the tearable annular connecting band, and when using, two annular connecting bands are torn, and the middle cell enrichment spare can be quickly separated and obtained.

The invention discloses a method for observing cyanobacteria cell communication by using an FRAP technology, and relates to the technical field of biological research, and the method comprises the following steps: culturing cyanobacteria; pretreating the cells; performing fluorescent dyeing and washing; fixing the sample; carrying out sample adaptation treatment; carrying out FRAP experiment observation; controlling an observation program; extracting and preprocessing data; processing and analyzing data; and verifying and applying a result. The problem of sample observation displacement is thoroughly solved through an innovative agar back-off fixation method, background fluorescence interference is eliminated by combining cell synchronization enrichment and multiple rounds of mild washing, and imaging and bleaching parameters adaptive to cyanobacteria light sensitivity characteristics are customized to balance signal quality and cell activity; meanwhile, a complete analysis process from sample adaptation and program automation control to data noise reduction calibration and nonlinear fitting is established, core parameters of communication dynamics are accurately extracted, and stable, accurate and quantitative observation of the communication dynamic process between cyanobacteria cells is achieved.

The invention provides a method for enriching trace tumor cells in peripheral blood. The cell enrichment method comprises the following steps: step 1, adding a cell lysis solution into peripheral blood for lysis and centrifugation to obtain a white precipitate; 2, mixing the white precipitate with an incubation medium to obtain an incubation solution; 3, positive electromagnetic beads are added into the incubation solution, shaking incubation and magnetic suction washing are carried out, and captured cells are obtained; wherein the incubation medium is a PBS (Phosphate Buffer Solution) containing fetal calf serum. According to the method, firstly, a blood sample is pretreated, and meanwhile, a specific dispersion medium is selected when target cells are combined with the positive electromagnetic beads, so that the activity of trace tumor cells is well preserved, cell loss is avoided, and the dispersion stability of the positive electromagnetic beads is improved; the efficient and high-activity enrichment of trace tumor cells in a trace tumor cell clinical blood sample or an artificial simulated blood sample can be realized.

Described herein are methods for cell wash, isolation and cryopreservation of frozen umbilical cord blood which can allow for reprogramming iPSCs from cord blood. Also described herein are methods of cord blood unit processing and cell enrichment. The methods are in accordance with current good manufacturing practices.

The invention discloses a sampling mechanism and a cell enrichment device and method, and relates to the technical field of cell enrichment. The sampling mechanism comprises a membrane cylinder, a membrane cylinder coupler and a filter membrane, the membrane cylinder is of a structure with openings in the two ends, the filter membrane is fixed to the opening in one end of the membrane cylinder, and the membrane cylinder coupler can be coupled or decoupled with the other end of the membrane cylinder; a pressure control assembly is integrated on the membrane cylinder coupler, when the membrane cylinder coupler is coupled with the membrane cylinder, in the pretreatment stage, the pressure control assembly is used for keeping negative pressure in the membrane cylinder and extracting the sample liquid at the same time, and in the film preparation stage, the pressure control assembly is used for keeping positive pressure in the membrane cylinder. The sampling mechanism has the dual technical advantages that firstly, the complex step of overturning a traditional membrane cylinder to suck sample liquid is cancelled; and secondly, the overall structure of the cell enrichment device is simplified. According to the sampling mechanism, the cell enrichment efficiency can be effectively improved, cross contamination among samples can be prevented, and a more efficient and safer solution is provided for biological sample treatment.

This invention discloses a method for preparing targeted magnetic microspheres with a bilayer structure and their applications, belonging to the fields of biomaterials and medical technology. Magnetic particles, dimethyloctadecyl[3-trimethoxysilylpropyl]ammonium chloride, 1,2-dioleoyl-sn-glycerol-3-phosphocholine, carboxymethyl chitosan hexadecyl quaternary ammonium salt, phospholipid-polyethylene glycol-amino group, and cholesterol are added to dichloromethane, and an emulsification reaction is carried out under ultrasonic conditions to obtain magnetic microspheres. Targeted magnetic microspheres can be prepared by adding antibodies or peptides after reaction. These targeted magnetic microspheres can be used in protein purification, nucleic acid extraction and separation, in vitro target cell sorting, in vivo target cell enrichment, targeted drug delivery, exosome sorting, drug delivery, drug carriers, targeted drug therapy, gene drug delivery, gene carrier construction, and magnetic resonance imaging contrast agents. The targeted magnetic microspheres have a bilayer structure, are positively charged, and exhibit superparamagnetism, biocompatibility, and safety.

1. Name of the product in this design: Enrichment Staining Apparatus. 2. Purpose of this design: For use in cell enrichment staining equipment. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key features: the front view.