87 results about "Cell enrichment" patented technology

The invention belongs to the technical field of breast cancer treatment, and particularly relates to a patient layering method based on a breast cancer single cell space proteome multicellular enrichment mode, which comprises the following steps: S1, breast cancer single cell space proteome image data cell segmentation and cell type identification; s2, constructing a cell network diagram, and simulating a tumor microenvironment; s3, detecting closely connected cell communities, and extracting cell community characteristics; s4, carrying out cell community feature clustering, and representing a tumor microenvironment distribution mode of the patient; and S5, calculating the similarity of the tumor microenvironment distribution modes of the patients, and layering the patients. By means of the method, the patients can be layered, and prognosis of the patient groups has significant difference.

The invention relates to the technical field of PCR (Polymerase Chain Reaction) detection, and discloses a high-risk human papilloma virus DNA (Deoxyribose Nucleic Acid) multiplex PCR detection kit, which adopts a primer probe group and comprises a PCR mixed solution, a negative reference substance and a positive reference substance, wherein the PCR mixed solution comprises a primer probe group. Female first-stage urine is used as a detection sample, and compared with a cervix uteri sample, sampling is more convenient, the experience feeling is better, and the willingness of female cervical cancer screening can be provided; meanwhile, the content of cervical exfoliated cells in the urine is also increased by using the first-stage urine, and the clinical sensitivity of clinical HPV detection can be remarkably improved by matching with a provided cell enrichment method.

The invention relates to the technical field of cell slide making, in particular to a full-automatic liquid-based cell slide making machine which comprises a transfer module, a cell enrichment module, a pipette tip module and a slide making module which are sequentially arranged. And the transfer module is arranged above the cell enrichment module, the pipette head module and the slide preparation module. The transferring module comprises a mechanical arm and a liquid sucking and injecting mechanism, and the liquid sucking and injecting mechanism is fixedly installed on the mechanical arm. The cell enrichment module comprises a centrifugal rotating disc, a first driving device, a waste liquid pouring device and a self-balancing assembly. The waste liquid dumping device is composed of a sample bottle push rod, and the sample bottle push rod can push the sample bottle bearing seat to enable the sample bottle bearing seat to swing along the shaft. Through cooperative arrangement of the transfer module with the liquid suction and injection mechanism and the cell enrichment module, the pelleter which is high in automation degree, compact and simple in structure and high in success rate is obtained.

The utility model relates to the technical field of biological medicine, in particular to a liquid suction device of CTC enrichment dyeing equipment, which comprises a mounting frame, a moving component is arranged at the upper end of the mounting frame, a liquid suction component is mounted on the moving component, and the liquid suction component moves along with the moving component in the vertical direction and the horizontal direction. The liquid suction assembly comprises a limiting frame and a plurality of liquid suction devices installed in the limiting frame, and the lower ends of the liquid suction devices are connected with liquid suction heads in a sleeved mode. According to the utility model, the problem of low efficiency of manual reagent adding in the CTC cell enrichment dyeing process is solved.

The invention relates to the technical field of medical inspection equipment, in particular to a CTC enrichment dyeing method and equipment. The method comprises the following steps: fixing a plurality of test tubes filled with enriched staining reagents on a movable test tube rack, placing a plurality of micro-fluidic chips on a movable chip assembly bracket according to the distance between the test tubes on the test tube rack, and arranging a heating platform below the chip assembly bracket; moving the test tube rack to a position above the chip assembly bracket, dumping the test tube rack, and simultaneously scattering the reagents in the plurality of test tubes on the plurality of micro-fluidic chips in a one-to-one correspondence manner; horizontally moving the chip assembly bracket, and blocking the liquid absorption assembly below the micro-fluidic chip by utilizing the edge of the heating platform, so that the liquid absorption assembly falls off from the micro-fluidic chip; and the micro-fluidic chip separated from the liquid absorption assembly is placed on a heating platform to be dried. The method is high in cell enrichment and dyeing operation efficiency, is especially suitable for cell enrichment and dyeing operation of batch CTC chips, and improves the CTC detection efficiency.

The invention discloses a PD-1 treatment responsiveness-oriented tumor microenvironment targeting cell enrichment method for a gastric cancer patient. On the basis that a ligand-receptor shows more significant correlation to the change of the expression level of VEGFA-FLT1 in the tumor tissue of the gastric cancer and the responsiveness of immunotherapy, a sufficient number of Mast cells and Endothelial cells are separated and obtained from the tumor tissue of a patient with the gastric cancer, and the expression level change of VEGFA-FLT1 in the tumor tissue of the gastric cancer and the responsiveness of the Endothelial cells in the tumor tissue of the patient with the gastric cancer show more significant correlation. The method can be used for making up for the deficiency of a single marker in improving the accuracy of immunotherapy responsiveness evaluation, so that the realization of future precise individualized immunotherapy of gastric cancer is better promoted.

Described herein are methods for cell wash, isolation and cryopreservation of frozen umbilical cord blood which can allow for reprogramming iPSCs from cord blood. Also described herein are methods of cord blood unit processing and cell enrichment. The methods are in accordance with current good manufacturing practices.

The utility model discloses a full-automatic liquid-based thin-layer cell pelleter which is more convenient compared with a traditional sealing mode such as manual pressing and the like by adsorbing and fixing an opening and closing plate and an opening end through magnetic force of an electromagnet for the whole working environment, and meanwhile, an occlusal pad is additionally arranged to increase the sealing performance of an arc-shaped side surface, so that the production efficiency is improved. The stability of the temperature and the humidity of a working environment is ensured, the possibility that a sample is evaporated and dried is reduced, so that the uniformity of the sample is kept, the uniform mixing efficiency is improved by additionally arranging the uniform mixing mechanism, meanwhile, when the sample is output to the glass slide, a traditional needle center liquid outlet mode is changed into a circular ring liquid outlet mode, the uniform distribution of cells on the glass slide is ensured, and the cell quality is improved. The cell enrichment degree is ensured.

The present disclosure provides a method of culturing a cell of a mesenchymal cell lineage, the method comprising contacting the cell with a culture medium comprising a CSF1R kinase inhibitor. The present disclosure also provides a method of culturing cells from bone marrow and / or compact bone to enrich the cells with mesenchymal cell lineage cells comprising contacting the cells with a culture medium comprising a CSF1R kinase inhibitor. The disclosure also provides cell culture media comprising a CSF1R kinase inhibitor and useful for culturing cells of the mesenchymal cell lineage and / or enriching cells of the mesenchymal cell lineage.

The invention discloses a method for high-throughput screening of high-activity lactic acid bacteria based on multi-index fusion and application, and belongs to the field of lactic acid bacteria screening, the method comprises the steps of bacterial cell enrichment, single-cell suspension preparation, physiological status and activity evaluation of cells, flow cytometry analysis, cell sorting and strain identification; in the step of enrichment of the bacterial cells, a sample is activated and enriched by using an improved liquid culture medium and an improved solid culture medium, and activated and enriched bacterial suspension is obtained. The invention provides the method for detecting the activity of the fermentation product and the lactic acid bacteria stored for a long time, the method is convenient and rapid, the screening time is greatly shortened, and the screening efficiency is improved.

The invention relates to the technical field of cell enrichment and separation equipment, in particular to a specific cell enrichment and magnetic separation system based on immunomagnetic beads. In order to solve the problem that an existing classifier is inconvenient to use, the following technical scheme is provided: a shelving module is mounted on the outer wall of the classifier, a sorting column is placed on a shelving groove of the shelving module, and an auxiliary unit and an adjusting unit mounted in the classifier are mounted in the shelving module; a moving block is installed on the outer wall of the sealing block, a pull rope is installed on the outer wall of the moving block, a sliding block is installed at the other end of the pull rope, and a rotating plate is movably connected to the inner wall of the placing module through a pin shaft. The placement state of the permanent magnet when the permanent magnet is not used and in use is changed, so that magnetic dust particles cannot be adsorbed on the placement module; and after being installed, the separation column can move upwards to adjust the liquid dropping speed, so that the operation difficulty of magnetic separation is reduced.

The utility model relates to the technical field of medical inspection equipment, in particular to a rigid chip for CTC (circulating tumor cell) cell enrichment and dyeing integrated equipment. Comprising a reagent groove and a rigid chip fixing sheet arranged at the lower end of the reagent groove, a first through hole is formed in the rigid chip fixing sheet, a chip body is placed in the first through hole, the reagent groove is of a sheet structure with a liquid leakage hole longitudinally penetrating through the chip body, and the chip body is made of transparent rigid materials. A plurality of groups of long strip-shaped micropores are uniformly distributed on the chip body; and the micro holes in the chip body comprise transverse holes which are transversely arranged and vertical holes which are longitudinally arranged. The chip is uniform in aperture, strong in selectivity and less in filtrate residue, can effectively avoid the phenomenon of cell stacking or hardening caused by overlarge negative pressure during suction filtration, improves the quality of CTC cell enrichment dyeing, and can be used for automatic detection equipment.

The invention discloses cell enriching and dyeing integrated equipment which comprises a base; the enrichment assembly is arranged on the base and is used for enriching target cells; the dyeing assembly is arranged on the base and is used for dyeing target cells; the liquid adding assembly comprises a first guide rail arranged on the base, a first liquid adding pipe and a second liquid adding pipe, the first liquid adding pipe and the second liquid adding pipe are in sliding fit with the first guide rail, the first liquid adding pipe is used for adding a first reagent to the enrichment assembly, and the second liquid adding pipe is used for adding a second reagent to the dyeing assembly. The enrichment assembly and the dyeing assembly are integrated on the same base, the liquid adding assembly with the first liquid adding pipe and the second liquid adding pipe which can slide along the guide rail is arranged, the guide rail design of the liquid adding assembly ensures the accuracy and repeatability of liquid adding, mechanical operation of reagent adding is achieved, and the reagent adding efficiency is improved. The time and steps for manually taking and placing the reagent are obviously reduced, so that the efficiency of the whole cell enrichment, dyeing and subsequent identification process is effectively improved.

The present invention relates to a method and platform for generating CAR-T cells and processing biological cells within a single functionally closed container. The method involves introducing host cells into a sealed container with multiple sterile channels, performing genetic modifications, and maintaining the cells under controlled conditions monitored by real-time feedback until a desired fraction of genetically modified cells is obtained. The container also supports target cell separation using antibody or aptamer functionalized buoyant microbubbles, facilitating the separation of target cells from non-target cells during centrifugation. Waste separation and cell harvesting are performed using pneumatic pressure, enabling high purity cell recovery. The platform includes modules for cell enrichment, sequestration, and isolation, enabling high recovery and high purity of therapeutic cell populations. This integrated closed system approach ensures sterile conditions, efficient processing, and high quality cell products for advanced therapeutic applications.

The invention discloses a method for efficiently and directionally inducing IPS to be differentiated into pancreatic beta cells. According to the method, through staged signal regulation and strict cell screening, the directionality and the overall efficiency of differentiation are remarkably improved. The whole process pays attention to cell quality control from the initial stage, and a stable foundation is laid for subsequent differentiation by screening iPS cells with uniform morphology and high expression of pluripotent markers. Progressive signal molecule regulation is adopted in each stage, and an accurate differentiation path is formed from Activin A gradient adjustment during endoderm induction, synergistic effect of FGF10 and retinoic acid in pancreatic precursor cell differentiation and Wnt signal inhibition during endocrine precursor cell induction, so that the non-specific differentiation tendency is effectively reduced. Meanwhile, cell enrichment steps of key nodes, such as SOX17 positive cell screening in an endoderm stage and Ngn3 magnetic bead sorting of endocrine precursor cells, further improve the purity of a target cell population and avoid interference of parenchyma cells on subsequent function maturation.

The utility model relates to the technical field of cerebrospinal fluid cell enrichment, in particular to a device for enriching cerebrospinal fluid cells, which comprises a base and two side fixing frames, gallery bridge plates are arranged between the sides of the two side fixing frames, a movable plate is arranged between the two gallery bridge plates in a sliding manner, and the top end of the movable plate is lower than the gallery bridge plates. The top end of the movable plate is detachably connected with a slide capable of settling and enriching cerebrospinal fluid cells in an attached mode, the top end of the slide is flush with the gallery bridge plates, and a filter paper plate which can be fixed at any position of the top ends of the gallery bridge plates and can absorb non-cellular components is installed between the top ends of the two gallery bridge plates through a magnetic attraction structure. The center of the top end of the filter paper plate is propped against an enrichment barrel capable of injecting cerebrospinal fluid downwards. On the premise of ensuring that the filter paper plate is attached to the slide, the filter paper plate is respectively fixed by different structures, so that the filter paper plate is driven and separated by different structures in the subsequent separation process, the problem that the filter paper plate is difficult to strip due to liquid intervention is effectively avoided, and subsequent analysis and observation are facilitated.

The invention relates to the technical field of biological sample treatment, and discloses a biological sample pretreatment method and a glass slide. The method comprises the following steps: adhering a first sample section to an adhesion layer of a first glass slide comprising an adhesion layer and a transparent layer, then placing a second glass slide carrying a dyed section marked with a target area below the first glass slide, cutting the adhesion layer according to a mark on the dyed section, and obtaining a second sample section marked with the target area; the target adhesion part adheres to the target area of the first sample slice, and finally, impurity removal treatment is conducted on the target adhesion part, so that a target nucleic acid sample is obtained. According to the method provided by the invention, the target region and the non-target region of the sample slice pair can be more accurately separated by using a simple, convenient and cheap instrument, residues can be reduced, the DNA yield is improved, a relatively good target cell enrichment effect is achieved, and the accuracy of extracting a nucleic acid sample of the target cell from the sample slice is improved.

The invention provides a brain glioma immune microenvironment complexity assessment method and application, and the method comprises the following steps: obtaining m glioma samples, and carrying out transcriptome determination on each cell in each glioma sample to obtain transcriptional expression profile data of the cells; carrying out clustering analysis on transcriptional expression profile data of the single cells through K-means clustering, and preliminarily identifying lymphocytes and myeloid cells according to immune cell classical markers; carrying out clustering analysis on the identified transcription expression profile data of the lymphocytes and myeloid cells, and identifying n cell subgroups; calculating the enrichment score of the characteristic gene set of each cell subset in the glioma transcriptome sample, wherein the higher the score is, the higher the enrichment proportion of the cell subset in the sample is; and evaluating the complexity of immune cell enrichment in the glioma sample based on the enrichment score of the characteristic gene set. The immune cell infiltration complexity can be predicted, and the relationship between immune cell enrichment complexity and brain glioma prognosis can be identified.

The invention discloses a Dean flow sorting device and method for on-line quantitative detection of cow's milk somatic cells, and belongs to the technical field of microfluidic cell detection. The device comprises a real-time cow milk sample collection unit, a sample inlet throttle valve, a Den flow micro-fluidic chip, a somatic cell enrichment milk sample outlet throttle valve, a somatic cell deficiency milk sample outlet throttle valve, an enrichment sample impedance detection unit, a deficiency sample impedance detection unit, a data processing unit and an online display unit. Interference suppression is realized by utilizing a sorting function of a Den flow micro-fluidic chip, somatic cells in cow milk are separated from other components, interference of complex conditions such as ion components in cow milk, environment temperature and the like on impedance data is effectively eliminated through comparative detection of somatic cell enrichment samples and somatic cell deletion samples, and the accuracy of a detection result is ensured.

The invention discloses a fat-targeted photo-thermal weight-losing light diagnosis and treatment agent and application thereof, and belongs to the technical field of nano biomedical diagnosis and treatment. The end group of the light diagnosis and treatment agent is modified with six water-soluble cations, the core skeleton of the light diagnosis and treatment agent is a conjugated near-infrared two-zone dye, the light diagnosis and treatment agent with an adipocyte enrichment function is obtained by directly dissolving ionic water-soluble light diagnosis and treatment small molecules in water, the retention time of the light diagnosis and treatment agent in adipose tissues is prolonged, and the weight losing function is achieved after fat illumination.

The invention belongs to the technical field of biological sample treatment, and discloses a filtering, sorting and enriching device which comprises a resuspension mechanism, a filtering and sorting mechanism and a filter membrane assembly. The resuspension mechanism can contain a liquid sample or resuspension liquid; the filtering and sorting mechanism is detachably connected to the resuspension mechanism; the filter membrane assembly is arranged in the filtering and sorting mechanism and is provided with a plurality of filter holes, a liquid sample enters the filter holes from the enrichment side of the filter membrane assembly, liquid in the liquid sample flows out of the filter holes from the filtering side of the filter membrane assembly, and target cells in the liquid sample are enriched in the enrichment side of the filter membrane assembly and / or the filter holes; and resuspending the resuspension to obtain a cell suspension. According to the filtering, sorting and enriching device, the yield and purity of target cells can be effectively improved, rare cells are prevented from being lost in the separation process, and the activity of the obtained cells is kept.

This invention provides a slide and a method for enriching cells by circulating centrifugation. The structure includes: an outer cylinder; a top cover disposed on the upper part of the outer cylinder; an inner cylinder disposed on the inner side of the outer cylinder; a receiving cavity formed between the outer wall of the inner cylinder and the inner wall of the outer cylinder; a slide housed in the lower part of the inner cavity; a valve component formed by the valve component on the bottom wall of the inner cylinder; a first downward inflow hole and a first upward outflow hole, with at least one first downward inflow hole provided in the lower part of the inner cylinder; and at least one first upward outflow hole provided in the upper part of the inner cylinder; the inner cavity of the inner cylinder is connected to the receiving cavity through the first downward inflow hole and the first upward outflow hole. This invention, through its infinite circulation function, can greatly improve the cell enrichment rate. For bodily fluids such as urine and transudate, which have large volumes and very few cells, it can collect as many cells as possible onto a single slide, thereby improving the diagnostic rate.

The invention provides a culture method of a neurofibroma organ. The balling culture medium of the neurofibroma organ comprises EGF (epidermal growth factor) and FGF (fibroblast growth factor) with the concentration of 200-250ng The organoid culture method comprises the following steps: digesting neurofibroma tissue blocks to prepare a single-cell suspension; the single-cell suspension is placed in a culture dish treated by polylysine, a Schwann cell culture medium is added to be cultured for 24 hours, the Schwann cell culture medium contains forsklin and beta-heragulin needed by Schwann cell enrichment, and Schwann cell enrichment liquid is obtained; collecting Schwann cell enrichment liquid, adding the balling culture medium and Y-27632, culturing for 6-12 hours, collecting cells, adding the balling culture medium, and culturing for 7-14 days. The tumor ball culture method comprises the following steps: culturing the organoid according to the method, digesting the organoid and culturing. According to the present invention, the Schwann cell screening step is added, the components of the balling culture medium are improved, and the Y-27632 is added, such that the problems of small tumor ball number and small size are solved, and the stable neurofibroma organ can be formed.

The present invention discloses a nuclear pore membrane biochip multiple detection device, including a collection device and a detection device, the collection device includes a first nuclear pore membrane, the first surface of the first nuclear pore membrane faces the body fluid flowing through the first nuclear pore membrane, so as to enrich the cells used for detection in the body fluid on the first surface; the detection device includes a preservation liquid cup containing a preservation liquid, the first surface of the first nuclear pore membrane can be immersed in the preservation liquid cup, and the preservation liquid is used to protect the cell morphology; the second nuclear pore membrane is detachably arranged at the bottom of the preservation liquid cup, and is used to filter the preservation liquid to enrich the cells in the preservation liquid on the first side of the second nuclear pore membrane. The present invention uses the first nuclear pore membrane to enrich the cells to be detected in the body fluid, and transfers them to the preservation liquid for exfoliated cell morphology detection, and uses the second nuclear pore membrane to enrich the cells in the preservation liquid for immunodetection or FISH detection, so as to realize multiple detection in combination with the exfoliated cell morphology detection, and more accurately detect the lesions in the body.

The invention discloses nucleated cell enriching and dyeing integrated equipment and method, and the integrated equipment comprises an enriching and dyeing unit which comprises a microfluidic device and is used for receiving a blood sample to be detected and enriching a target substance from the blood sample; the blood sample adding unit is used for adding a blood sample to be detected into the microfluidic device; the reagent taking and placing unit is used for sucking a reagent and adding the reagent into the microfluidic device; the microfluidic device comprises: a sample introduction tube comprising a tube main body, the lower end of which is provided with a buckling part protruding from the side wall of the tube main body; the liquid accumulation lining table is arranged below the sample injection pipe; the buckling cover is arranged on the pipe main body in a sleeving manner, the buckling cover spirally descends to be in threaded connection with the liquid accumulation lining table, the buckling part is pressed downwards to be close to the liquid accumulation lining table, and the filtering assembly arranged between the buckling part and the liquid accumulation lining table is pressed tightly. The nucleated cell enriching and dyeing integrated equipment can complete the whole process from blood sample conveying to target substance enriching and dyeing, and is beneficial to improving the detection efficiency.

The invention relates to the technical field of medical inspection equipment, in particular to a suction filtration device for CTC enrichment staining, which comprises a fixing assembly and a micro-fluidic chip mounted on the fixing assembly, the micro-fluidic chip comprises a reagent groove and a rigid chip fixing sheet arranged at the lower end of the reagent groove, the rigid chip fixing sheet is provided with a first through hole, and a second through hole is formed in the rigid chip fixing sheet. A chip body is placed in the first through hole, the reagent groove is of a sheet structure with a liquid leakage hole longitudinally penetrating through the body of the reagent groove, and a liquid suction assembly is arranged on the lower surface of the rigid chip fixing sheet. The suction filtration device adopts a rigid chip which is uniform in aperture and less in filtrate residue, so that the phenomenon of cell stacking or hardening caused by overlarge negative pressure during suction filtration can be effectively avoided, and the quality of cell enrichment and dyeing is improved; residual reagents in the chip can be removed by swinging and inclining the fixing assembly, so that automatic operation can be conveniently realized.

The present application relates to the technical field of biological cell collection and preservation, in particular to a peripheral blood immune cell efficient collection and low-temperature storage method, comprising: collecting peripheral blood samples and obtaining donor health status information, generating a sample cell composition atlas containing lymphocyte subpopulation absolute count percentage, monocyte purity and granulocyte contamination ratio through full-automatic blood cell analysis, determining the target immune cell optimal collection amount and adaptive collection process through a preset data analysis model, enriching target cells through gradient density centrifugation and specific magnetic bead sorting, comparing and judging supplementary collection by combining cell quality inspection data and expected collection amount, and then calculating individualized cryopreservation liquid formula, cooling program and storage strategy through a low-temperature storage optimization algorithm. The method can realize precise individualized adaptation of the collection process and storage parameters, and improve the cell enrichment purity and the cell state stability after low-temperature storage.

The invention belongs to the technical field of drug sensitivity detection, and particularly relates to a method for increasing the drug sensitivity detection speed of saccharomycetes and application of the method. The method comprises the steps of bacterial liquid preparation, sample adding, incubation, thallus enrichment, color developing agent adding and result interpretation, ethylenediamine tetraacetic acid or salt thereof needs to be added before thallus enrichment, and drug action and color developing are carried out separately. The detection method provided by the invention can effectively accelerate the developing speed of resazurin, remarkably shortens the drug sensitivity detection time, and is beneficial to early accurate diagnosis and treatment of clinical fungal diseases.

Human pluripotent stem cells (hPSCs), especially induced pluripotent stem cells (iPSCs) provide a promising starting material to produce mimetic innate immune cells such as natural killer (NK) cells and γδ T-cells for cancer immunotherapy. To facilitate consistent mass production, an overall manufacturing scheme to make mimetic innate immune cells from hPSCs was designed and demonstrated. Particularly, a robust protocol to differentiate hPSCs into NK cells or γδ T-cells through sequential hematopoietic differentiation on stromal cell line deficient in expressing M-CSF and lymphoid commitment on stromal cell line deficient in expressing M-CSF ectopically expressing DLL1 without employing CD34+ cell enrichment and spin embryoid body formation is established. Using this two-stage protocol, the generation of functional mimetic NK cells and functional mimetic γδ NKT-cells was demonstrated from hPSCs, including hESCs, peripheral blood cell-derived iPSCs (PBC-iPSCs). non-T cell-derived iPSCs or γδ T cell-derived iPSCs and the use of these mimetic innate immune cells in killing cancer cells.

The present invention relates to the technical field of cell enrichment and sorting equipment, and in particular to a specific cell enrichment and magnetic sorting system based on immunomagnetic beads. The present invention mainly addresses the inconvenience of using existing sorters and proposes the following technical solutions: the shelf module is mounted on the outer wall of the sorter, the sorting column is placed on the shelf slot of the shelf module, an auxiliary unit is installed inside the shelf module, and an adjustment unit is installed inside the sorter, the outer wall of the closing block is mounted with a moving block, the outer wall of the moving block is mounted with a pull rope, the other end of the pull rope is mounted with a slider, and the inner wall of the shelf module is movably connected to a rotating plate via a pin; the present invention changes the placement state of the permanent magnet when it is not in use and when it is in use, so that magnetic dust particles are not adsorbed on the shelf module; the permanent magnet can be firmly clamped when the sorting column is installed, and the sorting column can be moved upward after installation to adjust the dripping speed, thereby reducing the operational difficulty of magnetic sorting.