42 results about "Cell enrichment" patented technology

The utility model relates to the technical field of biological medicine, in particular to a liquid suction device of CTC enrichment dyeing equipment, which comprises a mounting frame, a moving component is arranged at the upper end of the mounting frame, a liquid suction component is mounted on the moving component, and the liquid suction component moves along with the moving component in the vertical direction and the horizontal direction. The liquid suction assembly comprises a limiting frame and a plurality of liquid suction devices installed in the limiting frame, and the lower ends of the liquid suction devices are connected with liquid suction heads in a sleeved mode. According to the utility model, the problem of low efficiency of manual reagent adding in the CTC cell enrichment dyeing process is solved.

Described herein are methods for cell wash, isolation and cryopreservation of frozen umbilical cord blood which can allow for reprogramming iPSCs from cord blood. Also described herein are methods of cord blood unit processing and cell enrichment. The methods are in accordance with current good manufacturing practices.

The utility model discloses a full-automatic liquid-based thin-layer cell pelleter which is more convenient compared with a traditional sealing mode such as manual pressing and the like by adsorbing and fixing an opening and closing plate and an opening end through magnetic force of an electromagnet for the whole working environment, and meanwhile, an occlusal pad is additionally arranged to increase the sealing performance of an arc-shaped side surface, so that the production efficiency is improved. The stability of the temperature and the humidity of a working environment is ensured, the possibility that a sample is evaporated and dried is reduced, so that the uniformity of the sample is kept, the uniform mixing efficiency is improved by additionally arranging the uniform mixing mechanism, meanwhile, when the sample is output to the glass slide, a traditional needle center liquid outlet mode is changed into a circular ring liquid outlet mode, the uniform distribution of cells on the glass slide is ensured, and the cell quality is improved. The cell enrichment degree is ensured.

The invention discloses a method for high-throughput screening of high-activity lactic acid bacteria based on multi-index fusion and application, and belongs to the field of lactic acid bacteria screening, the method comprises the steps of bacterial cell enrichment, single-cell suspension preparation, physiological status and activity evaluation of cells, flow cytometry analysis, cell sorting and strain identification; in the step of enrichment of the bacterial cells, a sample is activated and enriched by using an improved liquid culture medium and an improved solid culture medium, and activated and enriched bacterial suspension is obtained. The invention provides the method for detecting the activity of the fermentation product and the lactic acid bacteria stored for a long time, the method is convenient and rapid, the screening time is greatly shortened, and the screening efficiency is improved.

The utility model relates to the technical field of medical inspection equipment, in particular to a rigid chip for CTC (circulating tumor cell) cell enrichment and dyeing integrated equipment. Comprising a reagent groove and a rigid chip fixing sheet arranged at the lower end of the reagent groove, a first through hole is formed in the rigid chip fixing sheet, a chip body is placed in the first through hole, the reagent groove is of a sheet structure with a liquid leakage hole longitudinally penetrating through the chip body, and the chip body is made of transparent rigid materials. A plurality of groups of long strip-shaped micropores are uniformly distributed on the chip body; and the micro holes in the chip body comprise transverse holes which are transversely arranged and vertical holes which are longitudinally arranged. The chip is uniform in aperture, strong in selectivity and less in filtrate residue, can effectively avoid the phenomenon of cell stacking or hardening caused by overlarge negative pressure during suction filtration, improves the quality of CTC cell enrichment dyeing, and can be used for automatic detection equipment.

The invention discloses cell enriching and dyeing integrated equipment which comprises a base; the enrichment assembly is arranged on the base and is used for enriching target cells; the dyeing assembly is arranged on the base and is used for dyeing target cells; the liquid adding assembly comprises a first guide rail arranged on the base, a first liquid adding pipe and a second liquid adding pipe, the first liquid adding pipe and the second liquid adding pipe are in sliding fit with the first guide rail, the first liquid adding pipe is used for adding a first reagent to the enrichment assembly, and the second liquid adding pipe is used for adding a second reagent to the dyeing assembly. The enrichment assembly and the dyeing assembly are integrated on the same base, the liquid adding assembly with the first liquid adding pipe and the second liquid adding pipe which can slide along the guide rail is arranged, the guide rail design of the liquid adding assembly ensures the accuracy and repeatability of liquid adding, mechanical operation of reagent adding is achieved, and the reagent adding efficiency is improved. The time and steps for manually taking and placing the reagent are obviously reduced, so that the efficiency of the whole cell enrichment, dyeing and subsequent identification process is effectively improved.

The present invention relates to a method and platform for generating CAR-T cells and processing biological cells within a single functionally closed container. The method involves introducing host cells into a sealed container with multiple sterile channels, performing genetic modifications, and maintaining the cells under controlled conditions monitored by real-time feedback until a desired fraction of genetically modified cells is obtained. The container also supports target cell separation using antibody or aptamer functionalized buoyant microbubbles, facilitating the separation of target cells from non-target cells during centrifugation. Waste separation and cell harvesting are performed using pneumatic pressure, enabling high purity cell recovery. The platform includes modules for cell enrichment, sequestration, and isolation, enabling high recovery and high purity of therapeutic cell populations. This integrated closed system approach ensures sterile conditions, efficient processing, and high quality cell products for advanced therapeutic applications.

The invention discloses a method for efficiently and directionally inducing IPS to be differentiated into pancreatic beta cells. According to the method, through staged signal regulation and strict cell screening, the directionality and the overall efficiency of differentiation are remarkably improved. The whole process pays attention to cell quality control from the initial stage, and a stable foundation is laid for subsequent differentiation by screening iPS cells with uniform morphology and high expression of pluripotent markers. Progressive signal molecule regulation is adopted in each stage, and an accurate differentiation path is formed from Activin A gradient adjustment during endoderm induction, synergistic effect of FGF10 and retinoic acid in pancreatic precursor cell differentiation and Wnt signal inhibition during endocrine precursor cell induction, so that the non-specific differentiation tendency is effectively reduced. Meanwhile, cell enrichment steps of key nodes, such as SOX17 positive cell screening in an endoderm stage and Ngn3 magnetic bead sorting of endocrine precursor cells, further improve the purity of a target cell population and avoid interference of parenchyma cells on subsequent function maturation.

The invention provides a brain glioma immune microenvironment complexity assessment method and application, and the method comprises the following steps: obtaining m glioma samples, and carrying out transcriptome determination on each cell in each glioma sample to obtain transcriptional expression profile data of the cells; carrying out clustering analysis on transcriptional expression profile data of the single cells through K-means clustering, and preliminarily identifying lymphocytes and myeloid cells according to immune cell classical markers; carrying out clustering analysis on the identified transcription expression profile data of the lymphocytes and myeloid cells, and identifying n cell subgroups; calculating the enrichment score of the characteristic gene set of each cell subset in the glioma transcriptome sample, wherein the higher the score is, the higher the enrichment proportion of the cell subset in the sample is; and evaluating the complexity of immune cell enrichment in the glioma sample based on the enrichment score of the characteristic gene set. The immune cell infiltration complexity can be predicted, and the relationship between immune cell enrichment complexity and brain glioma prognosis can be identified.

The invention belongs to the technical field of biological sample treatment, and discloses a filtering, sorting and enriching device which comprises a resuspension mechanism, a filtering and sorting mechanism and a filter membrane assembly. The resuspension mechanism can contain a liquid sample or resuspension liquid; the filtering and sorting mechanism is detachably connected to the resuspension mechanism; the filter membrane assembly is arranged in the filtering and sorting mechanism and is provided with a plurality of filter holes, a liquid sample enters the filter holes from the enrichment side of the filter membrane assembly, liquid in the liquid sample flows out of the filter holes from the filtering side of the filter membrane assembly, and target cells in the liquid sample are enriched in the enrichment side of the filter membrane assembly and / or the filter holes; and resuspending the resuspension to obtain a cell suspension. According to the filtering, sorting and enriching device, the yield and purity of target cells can be effectively improved, rare cells are prevented from being lost in the separation process, and the activity of the obtained cells is kept.

This invention provides a slide and a method for enriching cells by circulating centrifugation. The structure includes: an outer cylinder; a top cover disposed on the upper part of the outer cylinder; an inner cylinder disposed on the inner side of the outer cylinder; a receiving cavity formed between the outer wall of the inner cylinder and the inner wall of the outer cylinder; a slide housed in the lower part of the inner cavity; a valve component formed by the valve component on the bottom wall of the inner cylinder; a first downward inflow hole and a first upward outflow hole, with at least one first downward inflow hole provided in the lower part of the inner cylinder; and at least one first upward outflow hole provided in the upper part of the inner cylinder; the inner cavity of the inner cylinder is connected to the receiving cavity through the first downward inflow hole and the first upward outflow hole. This invention, through its infinite circulation function, can greatly improve the cell enrichment rate. For bodily fluids such as urine and transudate, which have large volumes and very few cells, it can collect as many cells as possible onto a single slide, thereby improving the diagnostic rate.

The present application relates to the technical field of biological cell collection and preservation, in particular to a peripheral blood immune cell efficient collection and low-temperature storage method, comprising: collecting peripheral blood samples and obtaining donor health status information, generating a sample cell composition atlas containing lymphocyte subpopulation absolute count percentage, monocyte purity and granulocyte contamination ratio through full-automatic blood cell analysis, determining the target immune cell optimal collection amount and adaptive collection process through a preset data analysis model, enriching target cells through gradient density centrifugation and specific magnetic bead sorting, comparing and judging supplementary collection by combining cell quality inspection data and expected collection amount, and then calculating individualized cryopreservation liquid formula, cooling program and storage strategy through a low-temperature storage optimization algorithm. The method can realize precise individualized adaptation of the collection process and storage parameters, and improve the cell enrichment purity and the cell state stability after low-temperature storage.

The invention belongs to the technical field of drug sensitivity detection, and particularly relates to a method for increasing the drug sensitivity detection speed of saccharomycetes and application of the method. The method comprises the steps of bacterial liquid preparation, sample adding, incubation, thallus enrichment, color developing agent adding and result interpretation, ethylenediamine tetraacetic acid or salt thereof needs to be added before thallus enrichment, and drug action and color developing are carried out separately. The detection method provided by the invention can effectively accelerate the developing speed of resazurin, remarkably shortens the drug sensitivity detection time, and is beneficial to early accurate diagnosis and treatment of clinical fungal diseases.

Human pluripotent stem cells (hPSCs), especially induced pluripotent stem cells (iPSCs) provide a promising starting material to produce mimetic innate immune cells such as natural killer (NK) cells and γδ T-cells for cancer immunotherapy. To facilitate consistent mass production, an overall manufacturing scheme to make mimetic innate immune cells from hPSCs was designed and demonstrated. Particularly, a robust protocol to differentiate hPSCs into NK cells or γδ T-cells through sequential hematopoietic differentiation on stromal cell line deficient in expressing M-CSF and lymphoid commitment on stromal cell line deficient in expressing M-CSF ectopically expressing DLL1 without employing CD34+ cell enrichment and spin embryoid body formation is established. Using this two-stage protocol, the generation of functional mimetic NK cells and functional mimetic γδ NKT-cells was demonstrated from hPSCs, including hESCs, peripheral blood cell-derived iPSCs (PBC-iPSCs). non-T cell-derived iPSCs or γδ T cell-derived iPSCs and the use of these mimetic innate immune cells in killing cancer cells.

The present invention relates to the technical field of cell enrichment and sorting equipment, and in particular to a specific cell enrichment and magnetic sorting system based on immunomagnetic beads. The present invention mainly addresses the inconvenience of using existing sorters and proposes the following technical solutions: the shelf module is mounted on the outer wall of the sorter, the sorting column is placed on the shelf slot of the shelf module, an auxiliary unit is installed inside the shelf module, and an adjustment unit is installed inside the sorter, the outer wall of the closing block is mounted with a moving block, the outer wall of the moving block is mounted with a pull rope, the other end of the pull rope is mounted with a slider, and the inner wall of the shelf module is movably connected to a rotating plate via a pin; the present invention changes the placement state of the permanent magnet when it is not in use and when it is in use, so that magnetic dust particles are not adsorbed on the shelf module; the permanent magnet can be firmly clamped when the sorting column is installed, and the sorting column can be moved upward after installation to adjust the dripping speed, thereby reducing the operational difficulty of magnetic sorting.

The present invention relates to an iNKT-TCR molecule, TCR-T cell, preparation and application targeting a lipid antigen and CD1d complex, and belongs to the field of genetic engineering and immunotechnology. The present invention utilizes specific iNKT amplification technology to amplify and sort iNKT cells, and obtains iNKT cell-enriched TCRs by sequencing analysis. In this study, iNKT cells were amplified by iNKT cell-specific activators, high-purity iNKT cells were obtained by magnetic bead sorting, and iNKT cell-enriched TCRs were accurately screened out based on sequencing analysis to ensure their accuracy and specificity. The NKT38 of the present invention has a higher killing effect on tumor cells that express CD1d and present endogenous lipid antigens, and has strong specificity: in vitro experiments show that iNKT-TCR-transfected NKT38 cells have a stronger killing effect on tumor cell lines that express CD1d and present endogenous lipid antigens, suggesting that it has a specific killing effect on tumors.

The utility model relates to cell culture bag technical field discloses a cell culture bag, including the bag body, the one side of bag body is provided with a plurality of connecting heads, a plurality of connecting head one side is connected with liquid inlet pipe subassembly, negative pressure needleless connector and cell enrichment and liquid supplementing device, cell enrichment and liquid supplementing device include the connecting pipe of setting up in corresponding connecting head one side, through setting up liquid inlet pipe subassembly, negative pressure needleless connector and cell enrichment and liquid supplementing device in the bag body one side, realized the integration function of liquid inlet, negative pressure operation, cell enrichment and liquid supplementing in cell culture process, the valve component design of cell enrichment and liquid supplementing device, can accurate control cell enrichment and culture medium supplement's process, avoid the interference caused by both simultaneously, guarantee the purity when cell enrichment and the sterile environment when liquid supplementing, thereby improve cell subculture expansion culture's efficiency and stability, reduce the operation step, reduce the pollution risk.

The present invention relates to the technical field of medical testing equipment, specifically a suction filtration device for CTC enrichment and staining, comprising a fixed assembly and a microfluidic chip mounted on the fixed assembly. The microfluidic chip includes a reagent tank and a rigid chip fixing plate disposed at the lower end of the reagent tank. The rigid chip fixing plate is provided with a through hole 1, within which the chip body is placed. The reagent tank is a sheet-like structure with a liquid leakage hole extending longitudinally through the body. The lower surface of the rigid chip fixing plate is provided with a liquid suction assembly. The suction filtration device utilizes a rigid chip with uniform pore size and low filtrate residue, effectively preventing cell stacking or compaction caused by excessive negative pressure during suction filtration, thereby improving the quality of cell enrichment and staining. The fixed assembly can remove residual reagents from the chip by swinging and tilting the fixed assembly, facilitating automated operation.

The present disclosure provides methods and compositions for site-specific CAR integration using delivery of DNA by non-homologous dependent targeted insertion (HITI) in primary human T cells. The methods provide higher cell yields than homologous directed repair (HDR) mediated gene insertion. The CAR T cells are enriched to a purity of about 80% using HITI CRISPER Post Enrichment (CEMENT) of a GMP grade agent, resulting in CAR + T cells in a therapeutically related dose range.

The utility model discloses a processing device for pathological cell wax blocks, which comprises a case and a tube carrying frame, a first cavity for accommodating a metal bath mechanism and a second cavity for accommodating a centrifugal mechanism are formed in the case, and a first cavity opening communicated with the first cavity and a second cavity opening communicated with the second cavity are formed in the case; the pipe carrying frame is arranged in the first cavity through the first cavity opening and connected with the metal bath mechanism in a heat conduction mode to complete metal bath, and then is arranged in the second cavity through the second cavity opening and connected with the centrifugal mechanism. According to the utility model, reagent melting and cell enrichment and cell tiling integration blocks can be realized on the same machine, so that pathological cell wax blocks are convenient to manufacture, and the experiment efficiency is improved.

To provide cassettes for use in automated cell engineering systems that include cell concentration filters for reducing fluid volume of a cell sample during or following automated processing, and automated cell engineering systems.SOLUTION: A cassette comprises: (a) a cell culture chamber; (b) a pump fluidly connected to the cell culture chamber; (c) a tangential flow filter fluidly connected to the pump, wherein the pump provides a retentate flow to the tangential flow filter, wherein a permeate flow of the tangential flow filter is controlled by a flow controller; (d) a satellite volume connected to the tangential flow filter, contained in the cassette; (e) a fluid pathway for recirculating the retentate flow back through the tangential flow filter; (f) a fixed volume waste collection chamber fluidly connected to the tangential flow filter; and (g) a cellular sample output fluidly connected to the tangential flow filter.SELECTED DRAWING: Figure 1

The invention relates to an enrichment method of anaerobic nitrate reduction microorganisms and application thereof, and belongs to the technical field of microorganisms. According to the enrichment method disclosed by the invention, active cells in a solid sample are extracted by utilizing a Nycodenz density gradient centrifugation method, and then the active cells are inoculated into an anaerobic nitrate culture medium to carry out directional enrichment on anaerobic nitrate reducing microorganisms. According to the method, active cells can be efficiently enriched, the influence of a matrix is effectively reduced, and meanwhile, compared with a conventional cell enrichment technology, the efficiency is higher, and the cost is lower.

The present application belongs to the technical field of breast cancer treatment, and particularly relates to a patient stratification method based on breast cancer single-cell spatial proteomics multi-cell enrichment mode, comprising the following steps: S1: breast cancer single-cell spatial proteomics image data cell segmentation and cell type identification; S2: constructing a cell network graph and simulating a tumor microenvironment; S3: detecting closely connected cell communities and extracting cell community features; S4: clustering the cell community features and representing a patient tumor microenvironment distribution mode; and S5: calculating the similarity of the patient tumor microenvironment distribution mode and stratifying the patient. The method can stratify the patient, and the prognosis of the patient groups is significantly different.

The invention relates to the technical field of tumor immunity, and discloses a sample treatment method for detecting a circulating mononuclear cell immune function and a tumor microenvironment macrophage immune function, which comprises the following steps: treating a peripheral blood sample and a tumor pathological tissue sample; according to the treatment method, the mononuclear cell purity is improved through CD14 magnetic bead sorting, the macrophage recovery rate is improved through mixed enzymatic hydrolysate, and the core improvement that mild reagents (a hypotonic hemolysis buffer solution and a BSA-containing stationary solution) protect the cell activity and the antigen integrity is achieved, so that the treatment method has the important significance in the aspects of the mononuclear cell purity, the macrophage recovery rate, the cell activity and the antigen fluorescence intensity. Compared with the prior art, the method provided by the invention has the advantages that the method provided by the invention has the advantages that the method provided by the invention is obviously superior to a conventional treatment method, the defects of low monocyte enrichment efficiency, poor macrophage recovery rate and insufficient cell activity and antigen integrity of the conventional method are overcome, and a higher-quality sample basis is provided for subsequent circulating monocyte subtype typing (Mo1-Mo4) and tumor microenvironment macrophage immune function detection.

The application discloses a centrifugal tube and assembly for making cell wax blocks. The centrifugal tube comprises a ball-sac-equipped inner thread top cover, a centrifugal tube body composed of a funnel part and a cell enrichment tube, a cell collection capsule, a bottom pad and a bottom-sealed inner thread cover. The cell collection capsule and the bottom pad are arranged inside the cell enrichment tube to collect cells and can be taken in and out of the lower opening of the cell enrichment tube. After installation, detection liquid is added to the centrifugal tube body, and the centrifugal tube body is centrifuged by a centrifugal machine. The cells in the liquid are settled in the collection capsule. The bottom thread cover is unscrewed, the ball sac is pressed, and the cell collection capsule with the internal cell sediment is discharged from the lower opening of the cell enrichment tube under the hydraulic action. Then, the cell collection capsule is taken with the capsule cap and is placed in 10% neutral formalin solution for fixation. The fixing solution gradually penetrates into the cell mass, and the cell sediment gradually forms a certain shape and hardness. In this way, the cell wax block is simple and convenient to make and is not limited by the small amount of cells.

Disclosed herein are methods for single-cell sequencing. In some examples, the methods include enriching a sample comprising a plurality of cells for cells of interest to produce an enriched cell sample; isolating one or more cells of interest in the enriched cell sample; and obtaining sequence information of one or more polynucleotides from each of the one or more isolated cells. Obtaining sequence information may include generating a molecularly indexed polynucleotide library from the one or more isolated cells. Enriching the sample may include focusing cells of interest in the sample using acoustic focusing.

The invention discloses a high-throughput cell migration chip as well as a preparation method and an application method thereof. The high-throughput cell migration chip comprises a plurality of cell screening units, at least three injection ports, at least three migration channel outlets and a plurality of dampers, each cell screening unit comprises at least two cell culture chambers, at least two cell migration subgroup screening barriers and a cell enrichment chamber, and the cell enrichment chamber is separated from the cell culture chambers through the cell migration subgroup screening barriers; wherein the injection ports located on the two sides and the outlet of the migration channel are in fluid communication with the cell culture chamber, and the injection port located in the middle and the outlet of the migration channel are in fluid communication with the cell enrichment chamber; the damper is arranged in a flow channel system of the high-throughput cell migration chip, and the flow channel system is configured as follows: a flow channel structure comprising the damper is subjected to equal flow resistance design, so that a theoretical value of fluid pressure difference on two sides of a cell migration subgroup screening barrier is zero during fluid perfusion.

The present application relates to a kind of bionic lymph node organoids and its construction method, the organoids include in three-dimensional space: one B cell-rich core region;And one T cell-rich region, it is at least partially wrapped in the core region in space;Wherein, the core region can be differentiated to form the light area and dark area with structural difference in response to external stimulation.The method includes preparing first bioink and second bioink, the first bioink contains B lymphocyte, and the second bioink contains T lymphocyte;Using the first and second bioink, the organoid prototype with the spatial structure of T cell-rich region wrapping B cell-rich region is formed by three-dimensional construction technology;The organoid prototype is solidified to stabilize its structure;External stimulation is applied to the structure after solidification, and the B cell-rich region is differentiated to form light area and dark area.

The utility model discloses a multifunctional centrifugal tube, including pipe cover, tube body and pipe tail. The pipe cover is installed on the tube body, the pipe tail is detachably installed below the tube body, the pipe tail includes cell enrichment spare and pipe bottom spare, a plurality of hollow collection cavities are arranged on the cell enrichment spare, the collection cavity is connected with the tube body, the pipe bottom spare is detachably installed below the cell enrichment spare to block the bottom of the collection cavity, the pipe tail is divided into the upper and lower two parts of cell enrichment spare and pipe bottom spare, the connecting place of cell enrichment spare and pipe bottom spare is sealed through the tearable annular connecting band, and the connecting place of cell enrichment spare and tube body is sealed through the tearable annular connecting band, and when using, two annular connecting bands are torn, and the middle cell enrichment spare can be quickly separated and obtained.

The invention discloses a method for observing cyanobacteria cell communication by using an FRAP technology, and relates to the technical field of biological research, and the method comprises the following steps: culturing cyanobacteria; pretreating the cells; performing fluorescent dyeing and washing; fixing the sample; carrying out sample adaptation treatment; carrying out FRAP experiment observation; controlling an observation program; extracting and preprocessing data; processing and analyzing data; and verifying and applying a result. The problem of sample observation displacement is thoroughly solved through an innovative agar back-off fixation method, background fluorescence interference is eliminated by combining cell synchronization enrichment and multiple rounds of mild washing, and imaging and bleaching parameters adaptive to cyanobacteria light sensitivity characteristics are customized to balance signal quality and cell activity; meanwhile, a complete analysis process from sample adaptation and program automation control to data noise reduction calibration and nonlinear fitting is established, core parameters of communication dynamics are accurately extracted, and stable, accurate and quantitative observation of the communication dynamic process between cyanobacteria cells is achieved.