The invention belongs to the field of biological sample analysis and detection, and particularly relates to an integrated system for cell enrichment, separation, dyeing and flaking. The integrated system for cell enrichment, separation, dyeing and faking comprises an automatic control module, and a mechanical control device, a cell separation device and a specimen slide preparation device which can be controlled by the automatic control module in a linkage manner. According to the system disclosed by the invention, CTCs tumor cells are extracted by using the cell separation device in a filtering manner and then transferred to the specimen preparation device for dyeing treatment, and the whole operation process has relatively high automation degree and relatively high flux. All modules ofthe system are reasonable in layout and compact in structure; various functions of sample transferring, sample fixing, sample filtering, cell staining and sample sealing are integrated into one systemfor operation, the whole system is extremely high in integration degree, has the advantages of automation and high flux, can meet the requirement of individualized medical treatment, and is applied to various medical institution occasions, timely diagnosis and the like.