Microfluidic chip, apparatus for enriching cells and method for enriching cells in a microfluidic chip

Abstract

In accordance with an embodiment, a microfluidic chip including a first cells enrichment system and a second cells enrichment system is provided. Channel layouts of the first and the second cells enrichment systems are symmetric with respect to a reflection plane vertical to the microfluidic chip. Each of the first the second cells enrichment systems includes a first fluid channel, a second fluidchannel, a sample channel, an inlet channel, and a filtration chamber. The sample channel of the first cells enrichment system has a first inner wall and a first outer wall. The sample channel of thesecond cells enrichment system has a second inner wall and a second outer wall. The first outer wall and the second outer wall are both far from the reflection plane. A distance between the first outer wall and the second outer wall is in the range from about 10[mu]m to about 1cm.

Description

technical field

[0001] The present invention relates to techniques for enriching targets in fluid samples, and in particular, to a microfluidic chip, a device for enriching cells, and a method for enriching cells in a microfluidic chip. Background technique

[0002] A microfluidic chip is a chip-like device with one or more microfluidic channels that allow a fluid sample and / or desired reagents to travel therein such that the fluid sample can be tested in the channels of the microfluidic chip. Microfluidic chips have been used in various fields, especially biologically related fields such as biomedicine, biochemistry, or related fields. In applications in bio-related fields, blood samples are tested in microfluidic chips. Blood samples often contain a variety of cells, and the need to sort rare cells is expanding rapidly. Rare target cell populations include circulating tumor cells (circulating tumor cells, CTCs), hematopoietic stem cells (hematopoietic stem cells, HSCs) a...

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