220 results about "High oleic acid" patented technology

Compositions and methods related to transgenic high oleic acid / ALS inhibitor-tolerant soybean plants are provided. Specifically, the present invention provides soybean plants having a DP-305423-1 event which imparts a high oleic acid phenotype and tolerance to at least one ALS-inhibiting herbicide. The soybean plant harboring the DP-305423-1 event comprises genomic / transgene junctions having at least the polynucleotide sequence of SEQ ID NO:8, 9, 14, 15, 20, 21, 83 or 84. The characterization of the genomic insertion site of the DP-305423-1 event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the soybean DP-305423-1 events are provided.

Seeds, plants and oils are provided having high oleic acid; low linoleic acid; and low linoleic acid plus linolenic acid; and advantageous functional or nutritional properties. Plants are disclosed that contain a mutation in a delta-12 or delta-15 fatty acid desaturase gene. Preferred plants are rapeseed and sunflower plants. Plants carrying such mutant genes have altered fatty acid composition in seeds. In one embodiment, a plant contains a mutation in a region having the conserved motif His-Xaa-Xaa-Xaa-His, found in delta-12 and delta-15 fatty acid desaturases. A preferred motif has the sequence His-Glu-Cys-Gly-His. A preferred mutation in this motif has the amino acid sequence His-Lys-Cys-Gly-His. Nucleic acid fragments are disclosed that comprise a mutant delta-12 or delta-15 fatty acid desaturase gene sequence.

The invention discloses a method for nondestructively measuring main fatty acid content of peanut seeds. The method comprises the following steps of: based on the Fourier transform near-infrared diffuse reflection spectrum technology, scanning spectrums by adopting a best integrating sphere diffuse reflection mode for eliminating solid granule non-uniformity, establishing a multiple regression mathematical model by using full peanut seeds of multiple genotypes as standard sample sets, and predicting the main fatty acid content of an unknown sample through the model. The method is nondestructive, does not need to treat the sample, and does not harm the vitality and tissue structure of the seeds. The method has the advantages of simple operation, high sensitivity, high scanning speed, good signal-to-noise ratio and high measurement speed, and is suitable for high oleic acid quality breeding, germ plasm resource identification and inheritance rule research of peanuts.

This disclosure concerns a plant of the genus, Brassica, or parts thereof, which comprise one or more traits selected from the group consisting of high oleic acid content, low linolenic acid content, increased herbicide resistance, restorer of cytoplasmic male sterility, and increased clubroot disease (Plasmodiophora brassicae) resistance, compared to a wild-type plant of the same species. This disclosure further relates to wild-type and mutant alleles of genes involved in these traits, molecular markers linked thereto, and methods of their use.

High oleic acid triglyceride compositions that comprise fatty acid components of at least 75% oleic acid, less than 10% diunsaturated fatty acid component; less than 3% triunsaturated fatty acid component; and less than 8% saturated fatty acid component; and having the properties of a dielectric strength of at least 35 KV / 100 mil gap, a dissipation factor of less than 0.05% at 25NC, acidity of less than 0.03 mg KOH / g, electrical conductivity of less than 1 pS / m at 25NC, a flash point of at least 250NC and a pour point of at least −15NC are disclosed. Electrical insulation fluids comprising the triglyceride composition are disclosed. Electrical insulation fluids that comprise the triglyceride composition and a combination of additives are disclosed. Electrical apparatuses comprising the electrical insulation fluids and the use of electrical insulation fluids to provide insulation in electrical apparatuses are disclosed. A process for preparing the high oleic acid triglyceride composition is disclosed.

Seeds, plants and oils are provided having high oleic acid; low linoleic acid; and low linoleic acid plus linolenic acid; and advantageous functional or nutritional properties. Plants are disclosed that contain a mutation in a delta-12 or delta-15 fatty acid desaturase gene. Preferred plants are rapeseed and sunflower plants. Plants carrying such mutant genes have altered fatty acid composition in seeds. In one embodiment, a plant contains a mutation in a region having the conserved motif His-Xaa-Xaa-Xaa-His, found in delta-12 and delta-15 fatty acid desaturases. A preferred motif has the sequence His-Glu-Cys-Gly-His. A preferred mutation in this motif has the amino acid sequence His-Lys-Cys-Gly-His. Nucleic acid fragments are disclosed that comprise a mutant delta-12 or delta-15 fatty acid desaturase gene sequence.

Canola plants with high oleic acid and low linolenic acid content are disclosed. In addition, the canola plants of the invention are resistant to imidazolinone herbicides and are resistant to blackleg. The canola plants of the invention also have low total glucosinolate content. There is also provided a method for controlling weeds in a field of canola plants wherein the canola plants are herbicide resistant.

Seeds, plants and oils are provided having high oleic acid; low linoleic acid; and low linoleic acid plus linolenic acid; and advantageous functional or nutritional properties. Plants are disclosed that contain a mutation in a delta-12 or delta-15 fatty acid desaturase gene. Preferred plants are rapeseed and sunflower plants. Plants carrying such mutant genes have altered fatty acid composition in seeds. In one embodiment, a plant contains a mutation in a region having the conserved motif His-Xaa-Xaa-Xaa-His, found in delta-12 and delta-15 fatty acid desaturases. A preferred motif has the sequence His-Glu-Cys-Gly-His. A preferred mutation in this motif has the amino acid sequence His-Lys-Cys-Gly-His. Nucleic acid fragments are disclosed that comprise a mutant delta-12 or delta-15 fatty acid desaturase gene sequence.

In various aspects, the invention provides Brassica juncea plants, seeds, cells, nucleic acid sequences and oils. Edible oil derived from plants of the invention may have significantly higher oleic acid content than other B. juncea plants. In one embodiment, the B. juncea line MJ02-086-3 contains a mutant allele MJ02-086-3 / BjFAD2-a at the BjFAD2-a gene locus, having a premature stop codon, so that there are no functional copies of the FAD2 gene in MJ02-086-3 plants. Seeds from MJ02-086-3 plants may for example yield an oil having oleic acid content of greater than about 70% by weight.

In various aspects, the invention provides Brassica juncea plants, seeds, cells, nucleic acid sequences and oils. Edible oil derived from plants of the invention may have significantly higher oleic acid content than other B. juncea plants. In one embodiment, the B. juncea line MJ02-086-3 contains a mutant allele MJ02-086-3 / BjFAD2-a at the BjFAD2-a gene locus, having a premature stop codon, so that there are no functional copies of the FAD2 gene in MJ02-086-3 plants. Seeds from MJ02-086-3 plants may for example yield an oil having oleic acid content of greater than about 70% by weight.

The invention relates to a cereal meal replacement stick for fat loss and a preparing method thereof. The cereal meal replacement stick is prepared from concentrated whey protein, calcium caseinate, soybean isolate protein, hydrolysis fish collagen, konjac flour, psylliumseedhusk powder, a white kidney bean extract, a potato extract, conjugated linoleic acid glyceride powder, DSM fabuless palm oilpowder, freeze-dried fruit powder, beet juice powder, mixed cereal powder, mixed tocopherol concentrate, active probiotic powder, a compound nutrient supplement, freeze-dried fruit particles, nut grains, chia seeds, puffing cereal particles, puffing soybean protein grains, isomalto-oligosaccharide, fructo-oligosaccharide, resistant dextrin, glycerinum and high-oleic-acid sunflower seed oil. The preparing method specifically comprises the following steps of syrup boiling, material mixing, forming, cooling, cutting and coating or non-coating. The meal replacement stick has the advantages of energy negative balance, strong satiation, low sugar increase and full nutrition, and the health and vigor of the body are guaranteed while dieters lose weight and fat.

The invention belongs to the technical field of rape molecular breeding, in particular relates to a preparation method for a cabbage type rape high oleic acid codominant SCAR molecular marker and application as marker assisted selection in breeding high oleic acid cabbage type rape new products. The difference of nucleotide sequences of AA254 and AA177 is identified by cloning and checking sequences on DNA segments of fad2 gene of genomes of high oleic acid cabbage type rape strain AA254 and low oleic acid cabbage type rape strain AA177, primers YQ-Fad2a-1 and YQ-Fad2a-2 are developed by utilizing the difference of the nucleotide sequences provided by the invention, and then the codominant SCAR molecular marker which can distinguish cabbage type rape high oleic acid and low oleic acid is obtained. The invention provides a new marker for the rape molecular breeding. The invention also discloses the preparation method and the application of the molecular marker.

A food or beverage composition suitable for human consumption includes a cow's milk that has been supplemented and homogenized with an omega-3 fatty acid-containing milk supplementation oil, in which a milk supplementation oil includes one part by weight of an EPA / DHA fatty acid-containing enriching oil that has been combined and diluted with at least one part by weight of an oxidative stabilization oil, such as a low linoleic acid / high oleic acid-containing oxidative stabilization oil. As a result, the rate of oxidation of EPA / DHA fatty acids added to the milk via the milk supplementation oil can be reduced at least two-fold or even much more compared to the rate of oxidation of an equal quantity of the same EPA / DHA fatty acid-containing enriching oil homogenized into the same cow's milk without having been first combined and diluted with the oxidative stabilization oil.

The invention provides an oligopeptide immune-enhancing enteral nutrition emulsion and a preparation method thereof. The oligopeptide immune-enhancing enteral nutrition emulsion is prepared from maltodextrin, whey protein isolate, colza oil, high oleic acid sunflower seed oil, a ziziphus jujube extractive, a Chinese wolfberry fruit extractive, a pawpaw extractive, a kudzuvine root extractive, a poria cocos extractive, a mulberry extractive, a dandelion extractive, dietary fibers, mineral substances, vitamins, L-carnitine and taurine, an emulgator, citric acid fatty glyceride, and purified water through a mixing and concentrating method. The oligopeptide immune-enhancing enteral nutrition emulsion is capable of effectively improving the immunologic function of an organism through the combined action and synergistic interaction of high-content high-quality protein peptide and bioactive ingredients in medical and edible food materials, and can serve as a nutrition support for a cancer patient. The preparation method is simple, and can meet the requirement of large-scale industrialized production.

In various aspects, the invention provides Brassica juncea plants, seeds, cells, nucleic acid sequences and oils. Edible oil derived from plants of the invention may have significantly higher oleic acid content than other B. juncea plants. In one embodiment, the B. juncea line MJ02-357-3 contains a mutant allele MJ02-313-1 / BjFAD2-a at the BjFAD2-a gene locus, having a single base-pair change (a G to A substitution in the ORF at position 281 in reference to the first ATG start codon) relative to the wild type sequence. The change is predicted to encode a Glycine-94 Aspartic acid mutation in the sequence of the predicted BjFAD2-a protein. In another embodiment, the B. juncea line MJ02-357-3 contains a mutant allele MJ02-357-3 / BjFAD2-a at the BjFAD2-a gene locus, having a single base-pair change (a C to T substitution in the ORF at position 647 in reference to the first ATG start codon) relative to the wild type sequence. The change is predicted to encode a Proline-216 Leucine mutation in the sequence of the predicted BjFAD2-a protein. As a result of these mutations, it can be predicted that the function of the BjFAD2-a proteins are negatively affected in Brassica juncea lines MJ02-313-1 and MJ357-3 as reflected in the increased levels of oleic acid in seed oil in comparison with the wild-type line J96D-4830. Seeds from MJ02-313-1 and MJ02-357-3 plants may for example yield an oil having oleic acid content of greater than 70% by weight.

The invention discloses special blend oil for frying. The special blend oil for frying contains 10-50% by weight of high oleic acid peanut oil and 10-50% by weight of rice oil. The blend oil for frying is prepared by compounding any two or more than two of sunflower seed oil, olive oil, soybean oil, corn oil, cottonseed oil, palm oil, rapeseed oil, safflower oil, linseed oil and sesame oil in different proportions. The blend oil for frying is rich in fragrance, bright in color, high in content of oleic acid, rich in oryzanol and vitamin E, reasonable in composition of fatty acids and free of stickiness. Through a high-temperature frying test, the blend oil is high in smoke point, low in content of trans-fatty acids and high in frying stability. The fried food is small in oil content, good in crispy property, excellent in taste and nutritional and healthy. The blend oil disclosed by the invention can be used for frying different food materials, is suitable for being used as special fat for frying food in family and catering industry, is long in expiration date, and is novel nutritional and healthy special blend oil for frying.

The invention discloses a method for preparing 1, 3-dilinolein-2-palmitic acid triglyceride. The method includes: melting the catfish oil at the temperature of 50-60 DEGC for 30-60 min, reducing the temperature to 15-30 DEG C at the cooling rate of 1-5 DEG C / min for 5-48 h, eliminating the liquid oil through the filtering mode and obtaining the catfish oil solid lipid which is rich in palmitic acid; and taking sn-1, 3-digit specificity lipase as the catalyst and the free fatty acid which comes from high oleic acid vegetable oil as the acyl donor, conducting acidolysis on the catfish oil solid lipid, eliminating the free fatty acid and obtaining the product which is rich in 1, 3-dilinolein-2-palmitic acid triglyceride. The 1, 3-dilinolein-2-palmitic acid triglyceride is low in price of raw materials, high in content and capable of being applied to infant formula milk as the additive.

The invention discloses environmental-friendly easily-decomposed plant oil-based lubricating oil and a production process thereof and belongs to the field of lubricating oil. The lubricating oil uses plant oil as basic oil, wherein the mass ratio of the basic oil and an additive is 7: 3; the basic oil comprises high oleic acid sunflower seed oil and castor oil in a mass ratio of 6: 4. The content of oleic acid in the high oleic acid sunflower seed oil reaches 90% and the high oleic acid sunflower seed oil has an extremely high antioxidant property. The castor oil has ideal viscosity. The two are matched in used as ideal plant oil without special processing, so that the lubricating oil not only has the advantageous characteristic that conventional mineral oil is taken as the basic oil, but also no special processing is required by virtue of assistant modification of the additive. The lubricating oil can be put into use through simple proportioning and high-temperature smelting, and the lubricating oil is low in production cost and easy to popularize.

The invention provides a breeding method for high-oleic acid high-yield peanut, which includes steps: (1) coordinate parent combination: selecting high-yield germplasm with good integrated character and germplasm with high oleic acid content SPI098 to combine patent; (2) high oleic acid pressure screening: determining oleic acid of each seed in excellent single plant in early separation generation, and selecting excellent peanut seed with high oleic acid content; (3) high-yield pressure screening: planting the screened peanut seed with high oleic acid content in next generation, separating generation in anaphase, and selecting excellent high-yield single plant; and (4) high-oleic acid high-yield peanut variety or strain obtaining: obtaining novel high-oleic acid high-yield peanut variety or strain through multi-generation high-oleic acid high-yield pressure screening. The breeding method provided by the present invention directly screens according to character of oleic acid content in peanut seed, and has advantages of short breeding period, small blindness, high efficiency, and good economic benefit and social benefit.

The present invention relates to plants, seeds and products derived thereof, in particular to Brassica plants, seeds products derived thereof, that have mutant sequences conferring high oleic acid profile on the seed oil. More particularly, the invention relates to mutant delta-12 fatty acid desaturase sequences, also referred to herein as FAD2 sequences, —in-such-plants which confer high oleic acid profile on the seed oil.

The invention belongs to the technical fields of biology and food and discloses a production process of high oleic acid superfine fragrant peanut oil. The production process of the high oleic acid superfine fragrant peanut oil comprises the following steps: 1) choosing raw materials; 2) cleaning; 3) removing red skins; 4) preparing a natural compound antioxidant; 5) roasting seeds; 6) squeezing; 7) filtering at low temperature; 8) storing peanut oil. The invention also discloses peanut oil prepared by utilizing the production process. The production process of the high oleic acid superfine fragrant peanut oil has the advantages that the oxidation stability of oil is improved, and nutritional ingredients of the peanut oil and rich original mellow are reserved; a peanut red skin extract is used as an antioxidant, so that oleic acid can be avoided from being oxidized; meanwhile waste materials are changed into things of value, and the cost is saved.

The invention discloses a breeding method of a high-oleic-acid dormancy peanut variety. The breeding method of the high-oleic-acid dormancy peanut variety comprises the following steps: hybridizing parents, namely taking dragonhead No.2 as a female parent and taking a high-oleic-acid dormancy peanut variety system P76 as a male parent; screening high oleic acid by using pressure, namely harvesting single plants of F2-F3 hybridized generations of seeds, carrying out detection on the content of the nondestructive oleic acid in all the seeds of the single plates commonly and carrying out two generations of screening; screening high dormancy by using pressure; testing the dormancy and testing the content of the nondestructive oleic acid; and obtaining the high-oleic-acid dormancy peanut variety. The bred peanut variety is high in content of oleic acid and high in dormancy; the problem that sprouts grow on the plants when harvesting the peanuts can be effectively solved.

The invention relates to the technical field of preparation of peanut oil, in particular to a preparation method of high oleic acid virgin peanut oil, which is characterized by sequentially comprising the following steps: (1) screening varieties; (2) organically cultivating; (3) purchasing; (4) storing; (5) removing impurity; (6) drying at low temperature; (7) peeling; (8) breaking; (9) quenching; (10) cold pressing; (11) heating and stirring; and (12) centrifugally separating. The invention solves the problems that the existing pressing method has low oil extraction rate, high residual oil arte, poor smell of the peanut oil, and low content of oleic acid in the peanut oil. The invention has the following beneficial effects that: the production process is simple and easy to master and promote, and the production cost is low; and the nutrient content of the peanut oil is not damaged, the quality is better, and the nutrition value is higher. The method has higher oil extraction rate and lower residual oil rate.

The invention relates to nutritional compound oil with an anti-oxidation function. The nutritional compound oil consists of four or more of the following raw materials in parts by weight: 15-35 parts of soybean oil, 2-10 parts of corn oil, 2-5 parts of high-oleic acid sunflower seed oil, 50-65 parts of low-erucic acid rapeseed oil, 2-8 parts of coconut oil, 1-5 parts of linseed oil, 1-5 parts of walnut oil and 1-3 parts of black sesame oil; meanwhile, 0.01-0.02 part of L-ascorbyl palmitate and / or 0.03-0.05 part of vitamin E are added. According to the nutritional compound oil provided by the invention, by compounding multiple kinds of edible oil, the requirements of infants for the nutritional substances such as polyunsaturated fatty acids and vitamins are met, the nutrients are widely available, the processing process is simple, the production cost is low, the nutritional compound oil is convenient to eat, the content of trans-fatty acid components is low, and the fatty acids of omega-3 system and omega-6 system at a reasonable proportion are absorbed by a human body in a balanced manner.

Cost saving edible oil blends of plant or vegetable source comprising a low percentage by weight of at least one high oleic acid oil, a complementary high percentage by weight of at least one non- high oleic acid oil and antioxidant additives in an amount that does not affect organoleptic quality, the oil blends presenting a highly effective synergistic oxidation hindering effect at frying conditions.

Polyols which can be obtained by making epoxidized fatty acid esters of monofunctional C 1-8 alcohols react with aliphatic alcohols having a functionality ranging from 2-10. Fatty acid mixtures with mainly 18 carbon atoms represent the fatty acid component in the epoxidized fatty acid esters. The oleic acid content of the fatty acid mixtures is at least 80 wt %. The polyols are suitable for use in the production of isocyanate casting resins and coating compounds with enhanced colour stability.

The invention discloses a method for cultivating high oleic acid rapeseed by means of a gene editing technology, being characterized in that an FAD2 gene is site-directed edit by CRISPR / Cas9 system toobtain transgenic rapeseed plant, and comprising the follow steps: (1) selecting two gRNA target sites of FAD2 gene as S1 and S2 respectively, and synthesizing and construct vector-related primers; (2) constructing a double-target CRISPR / Cas9 gene editing vector; (3). extracting plasmid and transforming Agrobacterium tumefaciens; and (4) using Agrobacterium tumefaciens-mediated transformation totransfer the expression vector into rapeseed, and obtaining the transgenic plants with mutation of FAD2 gene. The oil content of the transgenic rapeseed plant is significantly higher than that of thewild-type rapeseed plant. Moreover, the method provided by the invention has high editing efficiency and good application prospect.

Compositions and methods related to transgenic high oleic acid / ALS inhibitor-tolerant soybean plants are provided. Specifically, the present invention provides soybean plants having a DP-305423-1 event which imparts a high oleic acid phenotype and tolerance to at least one ALS-inhibiting herbicide. The soybean plant harboring the DP-305423-1 event comprises genomic / transgene junctions having at least the polynucleotide sequence of SEQ ID NO:8, 9, 14, 15, 20, 21, 83 or 84. The characterization of the genomic insertion site of the DP-305423-1 event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the soybean DP-305423-1 events are provided.

The present invention belongs to the field of dielectric fluids for electric systems, it specifically relates to a biodegradable dielectric fluid that is highly resistant to oxidation consisting of an oil or a mixture of vegetable oils with a very high oleic acid content which substantially conserve all their natural tocopherols and containing a metal deactivator, as well as to its use for insulating and cooling electrical equipment.