Cabbage type rape high oleic acid molecular marker, preparation method and application thereof

Brassica napus and molecular marker technology, applied in the direction of botany equipment and methods, biochemical equipment and methods, applications, etc., can solve the problems of application limitations, achieve the goals of speeding up the process, reducing the breeding workload, and shortening the breeding period Effect

Active Publication Date: 2010-09-08
HUAZHONG AGRI UNIV
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AI Technical Summary

Problems solved by technology

According to these QTLs, the SNP markers related to fad2 were designed, but there was only one 3' end difference in the primer design of the obtained SNP markers, and the effect in Brassica napus linolenic acid-assisted selection breeding was affected by the concentration of DNA template, primers Synthetic quality, primer concentration, PCR reactio

Method used

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  • Cabbage type rape high oleic acid molecular marker, preparation method and application thereof
  • Cabbage type rape high oleic acid molecular marker, preparation method and application thereof
  • Cabbage type rape high oleic acid molecular marker, preparation method and application thereof

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Embodiment 1

[0040] 1. Establish double haploid (DH) population:

[0041] In this example, the high oleic acid strain A A254 was used as the female parent (the oleic acid content was 76.91%, and the seeds of this material were sent to the Chinese typical culture in Wuhan University, Wuhan City, Hubei Province on November 20, 2009. Preservation Center, its preservation number is CCTCC-P200909), common oleic acid content of rapeseed line A A177 (oleic acid content is 61.95%, the seeds of this material have been delivered to Wuhan University, Wuhan City, Hubei Province on November 20, 2009 F 1 , using F 1 The pollen of the plant is cultured through microspores (see the literature for microspore culture methods, Yu Fengqun, etc., some culture factors for improving the seedling rate of Brassica napus microspore embryoids, Acta Crops, 1997, 23 (2): 165- 168) 190 double haploid (DH for short) populations were obtained.

[0042] 2. Extraction of Brassica napus genomic DNA

[0043] Genomic DNA ...

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Abstract

The invention belongs to the technical field of rape molecular breeding, in particular relates to a preparation method for a cabbage type rape high oleic acid codominant SCAR molecular marker and application as marker assisted selection in breeding high oleic acid cabbage type rape new products. The difference of nucleotide sequences of AA254 and AA177 is identified by cloning and checking sequences on DNA segments of fad2 gene of genomes of high oleic acid cabbage type rape strain AA254 and low oleic acid cabbage type rape strain AA177, primers YQ-Fad2a-1 and YQ-Fad2a-2 are developed by utilizing the difference of the nucleotide sequences provided by the invention, and then the codominant SCAR molecular marker which can distinguish cabbage type rape high oleic acid and low oleic acid is obtained. The invention provides a new marker for the rape molecular breeding. The invention also discloses the preparation method and the application of the molecular marker.

Description

technical field [0001] The invention belongs to the technical field of molecular breeding of rapeseed, and in particular relates to the preparation and application of a high oleic acid molecular marker of Brassica napus. The molecular markers can be used as marker-assisted selection for breeding of high oleic rapeseed rape, and provide new molecular markers for cultivating genetically stable new varieties of high oleic rapeseed. Background technique [0002] The composition of fatty acids in rapeseed directly determines the quality of rapeseed oil and its nutritional value to humans. Oleic acid is an 18-carbon monounsaturated fatty acid, and rapeseed oil with high oleic acid content has high application value in edible oil and industry. The increase of monounsaturated fatty acids and the decrease of polyunsaturated fatty acids can increase the oxidation stability of edible oils and reduce oxidation products. When frying food, high oleic acid edible oil does not smoke at hi...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11C12P19/34A01H1/04
Inventor 周永明傅廷栋杨庆勇
Owner HUAZHONG AGRI UNIV
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