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4 results about "Background selection" patented technology
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Background selection describes the loss of genetic diversity at a non-deleterious locus due to negative selection against linked deleterious alleles. It is one form of linked selection, where the maintenance or removal of an allele from a population is dependent upon the alleles in its linkage group. The name emphasizes the fact that the genetic background, or genomic environment, of a neutral mutation has a significant impact on whether it will be preserved (genetic hitchhiking) or purged (background selection) from a population. In some cases, the term background selection is used broadly to refer to all forms of linked selection, but most often it is used only when neutral variation is reduced due to negative selection against deleterious mutations. Background selection and all forms of linked selection contradict the assumption of the neutral theory of molecular evolution that the fixation or loss of neutral alleles is entirely stochastic, the result of genetic drift. Instead, these models predict that neutral variation is correlated with the selective pressures acting on linked non-neutral genes, that neutral traits are not necessarily oblivious to selection. Because they segregate together, non-neutral mutations linked to neutral polymorphisms result in decreased levels of genetic variation relative to predictions of neutral evolution.
A method for efficient creation of a limited inflorescence Brassica napus line and a corresponding sterile line and application thereof
The application discloses a high-efficiency creating method and application of a limited inflorescence retention line and a corresponding sterile line of Brassica napus, and the method comprises the following steps: crossing a Polima CMS sterile line 1658A and a limited inflorescence resource 732 to obtain F1; continuously backcrossing the F1 and an unlimited inflorescence retention line C, performing foreground selection on limited inflorescence genes Bnsdt1 / Bnsdt2 by using a codominant molecular marker in each generation, and reserving a completely sterile single plant BC3F1; giving a low-temperature treatment at a budding stage of the BC3F1 generation to induce trace pollen, continuously backcrossing, and obtaining RBC4F1 and BC4F1; and self-crossing after foreground and background selection is performed again. The application solves the problems of a long period of breeding the limited inflorescence retention line and the sterile line in the prior art, and the problem that breeding of the sterile line must wait for completion of the retention line transformation, and the problem of low efficiency, and a limited inflorescence retention line and a corresponding sterile line of Brassica napus are efficiently created.
Owner:QINGHAI UNIVERSITY
Primer group for detecting PARMS molecular marker of rice plant height gene OsPH4 and application of primer group
PendingCN121826217AMicrobiological testing/measurementDNA/RNA fragmentationBiotechnologyEnzyme digestion
The invention belongs to the field of rice breeding, and particularly discloses a primer group for detecting a PARMS molecular marker of a rice plant height gene OsPH4 and application of the primer group, dwarf type OsPH4 gene detection is carried out on a rice material through a molecular sequence of the rice plant height gene OsPH4 by applying a PARMS technology, and the rice plant height gene OsPH4 is obtained. The rice dwarf type OsPH4 gene can be rapidly and accurately detected in different germplasm resources such as indica rice and japonica rice. According to the method, tedious procedures such as enzyme digestion, electrophoresis and sequencing are not needed in the detection process, pollution of PCR product aerosol and use of toxic substances such as EB are reduced, meanwhile, foreground selection and background selection can be conducted in the early stage of molecular marker assisted breeding, the background recovery rate is increased, the scale of breeding populations is reduced, the breeding process is accelerated, and the breeding efficiency is improved. The efficient and environment-friendly application of the OsPH4 gene in commercial molecular breeding of rice is facilitated.
Owner:LIAONING RICE RES INST
Technique for shortening breeding cycle of cotton and application thereof
ActiveCN119547727BMicrobiological testing/measurementSaving energy measuresBiotechnologyGenome resequencing
The application discloses a technology for shortening a cotton breeding cycle and application thereof, and comprises the following steps: 1, accelerating budding and flowering under mixed light composed of blue light, green light, red light and far-red light; 2, promoting germination of young embryos by GA3 treatment; 3, culturing cotton young embryos with an age of 25-28 days into seedlings by using L-mAUH and mAUH cotton young embryo culture medium; 4, repeating the above steps one to three for multiple times, and in the process, using molecular markers to assist in selecting good traits, and using whole genome resequencing to assist in background selection. The application has the advantages that by means of efficient blue, green, red and far-red spectrum regulation, combined with GA3 treatment, young embryo culture, molecular marker selection and whole genome resequencing, the growth and development of cotton can be significantly accelerated, and the cotton breeding cycle can be effectively shortened. The method can be effectively integrated into a conventional backcross breeding process, excellent traits can be quickly transformed, and an improved line with a high genetic background recovery rate can be obtained within 1.5 years.
Owner:HEBEI AGRICULTURAL UNIV.
Shanxi white pig whole genome SNP chip and application thereof
PendingCN121852547ANucleotide librariesMicrobiological testing/measurementMolecular breedingGenome resequencing
The invention discloses a Shanxi white pig whole genome SNP chip and application thereof, and on the basis of Shanxi white pig whole genome re-sequencing data, through GWAS screening verification, invalid SNP markers are removed, and 55957 Shanxi white pig specific SNP sites are determined. The Shanxi white pig whole genome SNP can be used for paternity test when Shanxi white pigs and Shanxi white pigs are parent varieties, pig clustering analysis when Shanxi white pigs and Shanxi white pigs are parent varieties, pig genetic relationship analysis, pig linkage map construction and gene localization and pig molecular breeding material background selection, and the pig selection and breeding process in China can be greatly improved.
Owner:SHANXI AGRI UNIV