Method for preparing 1, 3-dilinolein-2-palmitic acid triglyceride

A technology of palmitic acid triglyceride and dioleic acid, applied in the field of oils and fats, can solve the problems of complex process, difficult industrialized production and the like, and achieve the effects of wide sources, small proportion and low price

Active Publication Date: 2015-08-26
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are also people who use palm stearin as raw material to prepare OPO with higher purity by alcoholysis and re-esterification, but the process is more complicated and it is difficult to obtain industrial production.

Method used

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  • Method for preparing 1, 3-dilinolein-2-palmitic acid triglyceride
  • Method for preparing 1, 3-dilinolein-2-palmitic acid triglyceride
  • Method for preparing 1, 3-dilinolein-2-palmitic acid triglyceride

Examples

Experimental program
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Effect test

example 1

[0022] The refined freshwater catfish oil was melted at 60°C and kept for 30 minutes, then the catfish oil was cooled to 30°C at a cooling rate of 5°C / min, and crystallized for 8 hours, and the solid fat in the catfish oil was separated. The catfish oil is vacuum filtered to obtain the solid fat component. Fractionated solid fat contains higher sn-2 palmitic acid and total palmitic acid than catfish oil. The fatty acid composition and distribution of the used catfish oil and its fractionated solid fat are shown in the following table:

[0023] Table 1. Fatty acid composition and distribution of catfish oil and its fractionated solid fat

[0024]

[0025]

[0026] Catfish solid lipid components were acid-hydrolyzed in a batch reactor, using 1,3-specific lipase Lipozyme RMIM as a catalyst, using fatty acids derived from high oleic sunflower oil as acyl donors, and the molar ratio of catfish oil to fatty acids was 1:6, the reaction temperature is 50°C, the amount of enzym...

example 2

[0034] Melt the refined freshwater catfish oil at 50°C and keep it for 60 minutes, then lower the temperature of the catfish oil to 20°C at a cooling rate of 3°C / min, crystallize for 7 hours, and analyze the solid fat in the catfish oil. The catfish oil is vacuum filtered to obtain solid fat components. Fractionated solid fat contains higher sn-2 palmitic acid and total palmitic acid than catfish oil. The fatty acid composition and distribution of gained catfish oil fractionation solid fat are shown in table 4 below:

[0035] Table 4. Fatty acid composition and distribution of catfish oil fractionated and solidified at 20℃

[0036]

[0037] Acid hydrolysis of catfish solid lipid components in a packed bed continuous reactor, using 1,3-specific lipase Lipozyme TL IM as a catalyst, using fatty acids derived from high oleic rapeseed oil as acyl donors, catfish oil and fatty acids The molar ratio was 1:5, the reaction temperature was 40 °C, and the substrate residence time wa...

example 3

[0046]Refined freshwater catfish oil was melted at 55°C and kept for 50 minutes, then the catfish oil was cooled to 25°C at a cooling rate of 2°C / min, and crystallized for 10 hours, and the solid fat components in the catfish oil were separated. The catfish oil is vacuum filtered to obtain the solid fat component. Fractionated solid fat contains higher sn-2 palmitic acid and total palmitic acid than catfish oil. The fatty acid composition and distribution of gained catfish oil fractionation solid fat are as shown in table 7 below:

[0047] Table 7. Fatty acid composition and distribution of catfish oil fractionated and solidified at 25℃

[0048] FA type

FA

sn-2FA

%sn-2FA

sn-1,3FA

C12:0

0.33

0.59

59.60

0.20

C14:0

4.72

4.38

30.93

4.89

C16:0

34.88

58.89

56.28

22.88

C16:1

0.49

0.62

42.18

0.43

C18:0

9.22

4.88

17.64

11.39

C18:1

37.57...

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Abstract

The invention discloses a method for preparing 1, 3-dilinolein-2-palmitic acid triglyceride. The method includes: melting the catfish oil at the temperature of 50-60 DEGC for 30-60 min, reducing the temperature to 15-30 DEG C at the cooling rate of 1-5 DEG C/min for 5-48 h, eliminating the liquid oil through the filtering mode and obtaining the catfish oil solid lipid which is rich in palmitic acid; and taking sn-1, 3-digit specificity lipase as the catalyst and the free fatty acid which comes from high oleic acid vegetable oil as the acyl donor, conducting acidolysis on the catfish oil solid lipid, eliminating the free fatty acid and obtaining the product which is rich in 1, 3-dilinolein-2-palmitic acid triglyceride. The 1, 3-dilinolein-2-palmitic acid triglyceride is low in price of raw materials, high in content and capable of being applied to infant formula milk as the additive.

Description

technical field [0001] The invention belongs to the technical field of oils and fats, and in particular relates to a method for preparing a product rich in 1,3-dioleic acid-2-palmitic acid triglyceride by acid hydrolysis. Background technique [0002] Breast milk is the best food for infants and young children, providing almost all the nutrients needed by infants and young children. Among them, fat accounts for about 3-5% of breast milk, but provides more than 50% of the energy for infants and young children. Among these fats, the triglyceride content is above 98%. The content of palmitic acid in breast milk is 20-30%, a large amount of saturated fatty acid (palmitic acid) is in the sn-2 position of triglyceride, and unsaturated fatty acid is in the sn-1, 3 position, thus forming a large amount of sn-2 It is a saturated fatty acid, and sn-1,3 is a triglyceride of an unsaturated fatty acid, such as 1,3-dioleic acid-2-palmitic acid triglyceride (OPO). [0003] After the fat ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64
CPCA23C11/02A23D9/02C12P7/64C12P7/6454
Inventor 王兴国邹孝强金青哲黄健花刘睿杰常明王小三潘秋琴
Owner JIANGNAN UNIV
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