24 results about "Triglyceride content" patented technology

The invention discloses hippophae rhamnoides polysaccharide with the activity of reducing blood sugar and blood fat as well as a preparation method and application thereof. Adding water into the degreased hippophae rhamnoides, adjusting the pH value to be acidic, then heating, stirring and extracting under an ultrasonic condition, and collecting filtrate; and performing post-treatment to obtain the sea-buckthorn polysaccharide. The content of neutral sugar in the sea buckthorn polysaccharide is 25.31%-30.68%, the content of uronic acid in the sea buckthorn polysaccharide is 54.66%-59.79%, and the content of protein in the sea buckthorn polysaccharide is 1.91%-2.14%. According to the method, the sea-buckthorn polysaccharide is extracted by adopting acid assistance-ultrasonic extraction, and the prepared sea-buckthorn polysaccharide has remarkable blood sugar and blood fat reducing activity. When the concentration of the sea-buckthorn polysaccharide is as low as 0.1 mg / mL, the glucose uptake of HepG2-IR cells can still be obviously improved, and the content of total cholesterol and total triglyceride of the HepG2-IR cells can be obviously reduced. The hippophae rhamnoides polysaccharide can be applied to preparation of food or medicine for reducing blood sugar and / or blood fat.

This invention relates to a method for rapidly screening filamentous fungi that produce high levels of triglycerides and polyunsaturated fatty acids, belonging to the field of microbial technology. The method employs a two-stage screening strategy of "resistance primary screening – fluorescence secondary screening." In the primary screening stage, strains with strong lipid synthesis capabilities are initially screened by adding a combination of phytocyanin and quizalofop-p-ethyl inhibitors to the culture medium. In the secondary screening stage, cellophane culture technology and Nile red fluorescence staining are combined to quantitatively assess the triglyceride content of the colonies, achieving rapid and non-destructive screening. This method is simple to operate, highly efficient, and shows a significant positive correlation between fluorescence intensity and triglyceride content. It is suitable for mutagenesis breeding and industrial strain selection of oil-producing filamentous fungi such as *Morchella alpineensis*.

The invention belongs to the technical field of microorganisms, and discloses clostridium butyricum ML5358 and application thereof.The clostridium butyricum ML5358 provided by the invention has the preservation number of CGMCC No.37000. The strain can effectively relieve mouse colitis induced by DSS and remarkably reduce expression of colon tissue inflammatory factors Il-6 and Il-1beta, and the preservation number of the clostridium butyricum ML5358 is CGMCC No.37000. The clostridium butyricum ML5358 is CGMCC No.37000. The clostridium butyricum ML5358 is CGMCC No.37000. In addition, skeletal muscle lipid metabolism can be regulated and controlled through the intestine-muscle axis, expression of fat synthesis and uptake related genes Srebf1, Acc1 and Fabp4 is specifically up-regulated, the content of intramuscular triglyceride is remarkably increased, and intramuscular fat deposition is promoted. According to the clostridium butyricum ML5358 and the application thereof, the functional limitation of traditional clostridium butyricum is broken through, and a new strain resource is provided for developing a novel microbial preparation or a feed additive capable of improving intestinal health and improving livestock and poultry meat quality.

The invention provides a processing method of peanut butter, and belongs to the technical field of food processing. Peanut kernels are degreased through a hydraulic oil pressing device, the content of triglyceride in the peanut kernels is reduced, and product energy is reduced; in order to make up flavor loss caused by reduction of the grease content, enzyme and protease are adopted to conduct enzymolysis on degreased peanut kernels, the hydrolytic activity of protein and saccharides is improved, the content of free amino acid and reducing sugar is increased, and a flavor precursor is provided for Maillard reaction in the subsequent heat treatment process; the heat treatment can promote the generation of aroma substances and enable the flavor to be more harmonious; in order to ensure the quality of the peanut butter and improve the fluidity of the peanut butter, the crushed peanuts after heat treatment and the light-fat peanut oil are mixed and ground, so that the light-fat peanut oil is uniformly dispersed. Compared with traditional peanut triglyceride, the light-fat peanut oil has a lower metabolic energy value and good interfacial activity, not only can improve the rheological property, lubricity and structural stability of the peanut butter, but also can promote release and retention of flavor active substances.

This invention discloses a method for evaluating the bioavailability of soybean oil based on an in vitro digestion / cell co-culture model. Soybean oil is emulsified using WPI emulsifier and then digested sequentially through simulated gastric and intestinal fluids to obtain the gastric digestion products of soybean oil. Fatty acids are extracted from the intestinal digestion products of soybean oil, dissolved in dimethyl sulfoxide, and linked to fatty acid-free bovine serum albumin to obtain in vitro digestion products of soybean oil that can be absorbed by the intestines. These are then added to a cell co-culture model for cell incubation and transport. HepG2 cells from the cell co-culture model are collected, and Oil Red O staining and total triglyceride content are measured to obtain lipid deposition, thereby evaluating the bioavailability of soybean oil. The in vitro digestion / cell co-culture model of this invention is specifically designed for soybean oil digestion. This method objectively and scientifically evaluates the bioavailability of soybean oil at different metabolic stages, providing a standard basis for the scientific and healthy consumption of soybean oil and possessing significant practical value.

The invention discloses a method for identifying or assisting in identifying the content of duck sebum triglyceride, a related composition and application, and belongs to the field of nucleic acid-containing determination methods. The method for identifying or assisting in identifying the content of the duck sebum triglyceride comprises the following steps: detecting the genotype of a specific SNP (Single Nucleotide Polymorphism) site of a duck to be detected, and identifying or assisting in identifying the content of the duck sebum triglyceride according to the genotype of the specific SNP site of the duck to be detected. The specific SNP site is an SNP site in a duck genome, the nucleotide type of the specific SNP site is T or C, and the specific SNP site is the 70th nucleotide of SEQ ID No.1. The method can be used for carrying out early selection on the multi-triglyceride content character of the duck sebum, so that the duck breeding efficiency is improved, and the breeding period is shortened.

A process for producing a triglyceride-phospholipid concentrate is provided, the process comprising: providing an egg yolk lecithin solution from the extraction of an egg yolk material with a liquid extractant, mixing the egg yolk lecithin solution with an OPO triglyceride carrier comprising 1,3-dioleoyl-2-palmitoylglycerol and having a ratio of palmitic acid in the 2-position of the triglycerides relative to the total palmitic acid in the triglycerides (SN2-C16:0) of at least 50%, and a C52 triglyceride content of at least 42 wt.%, and separating the liquid extractant from the mixture to produce a triglyceride- phospholipid concentrate comprising the OPO triglyceride carrier and egg lecithin.

To provide an oil which does not become cloudy when stored at a low temperature while containing a specific amount of palmitic acid.SOLUTION: An oil containing a specific amount of palmitic acid has at least one characteristic selected from the group consisting of (i) a predetermined triglyceride content, (ii) a predetermined PUFA content, (iii) a predetermined stearic acid content, (iv) a predetermined content of C18 fatty acids, and (v) a predetermined DHA / EPA ratio. The oil does not become cloudy during low-temperature storage.SELECTED DRAWING: Figure 1

The invention relates to the technical field of coreopsis tinctoria refined components and preparation and application, and provides a coreopsis tinctoria refined component and a preparation method and application thereof.The coreopsis tinctoria refined component comprises, by mass, 11 specific components of phenolic acids, flavonol, flavanol and flavonoid, and the 11 specific components comprise, by mass, 2 parts of gallic acid, 1 part of potassium sorbate, 1 part of potassium sorbate, 1 part of potassium sorbate and 1 part of potassium sorbate. The health-care food is prepared from the following raw materials in parts by weight: 3 parts of chlorogenic acid, 14 parts of quercetin, 18 parts of catechin, 5 parts of citric acid, 2-3 parts of procyanidine B, 3 parts of (+)-epicatechin, 1-31 parts of 31 parts of procyanidine C, 14 parts of rutin, 4 parts of camphanol-3-O-rutinoside and 2 parts of isorhamnetin-3-O-rutinoside. The snow chrysanthemum is Changbai Mountain snow chrysanthemum. Animal experiments show that the refined component can significantly reduce the content of total cholesterol and triglyceride in serum of a high-fat model rat, the specific component with blood fat regulating activity is prepared from Changbai Mountain snow chrysanthemum, and a new choice is provided for non-drug intervention of dyslipidemia.

This invention provides a method for quantitative analysis of intracellular lipid content based on the Bodipy 493 / 503 fluorescent probe, relating to the field of lipid metabolism detection technology. The method includes: (1) determining the absorption spectrum and excitation / emission spectrum of Bodipy 493 / 503 in isopropanol, and determining the maximum absorption wavelength and maximum excitation / emission wavelength of the fluorescent probe in isopropanol based on the spectral results; (2) staining the target cells with the Bodipy 493 / 503 probe after treatment with the compound, observing the changes in fluorescence signal in the field of view before and after isopropanol extraction, and taking pictures; (3) measuring the absorbance and fluorescence intensity of the isopropanol extract, analyzing the experimental results, and comparing them with the results of triglyceride content detection. This quantitative method is simple, fast, sensitive, effective, and has good experimental controllability and repeatability. The analytical results obtained are consistent with those obtained using other lipid content analysis methods, and it can be of great application in the field of research on the lipid metabolism interference effects of chemicals or environmental pollutants.

The invention discloses lactobacillus plantarum ZYT028 and application thereof, the strain is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M 20251257, and the lactobacillus plantarum ZYT028 has good acid cholate tolerance and intestinal colonization ability, can still keep high survival rate under the condition of pH 2.0, and can grow in an environment containing 1.0 g / L cholate. The ZYT028 strain can promote the generation of short-chain fatty acid and improve the imbalance of intestinal flora caused by high fat diet; in animal experiments, the content of serum total cholesterol and triglyceride can be remarkably reduced, excessive weight increase is inhibited, and good lipid-lowering and weight-regulating effects are shown. The strain disclosed by the invention can be widely applied to development of functional foods, health-care products, fermented dairy products and probiotic preparations, and is used for assisting in controlling body weight and improving blood lipid metabolism.

The present application belongs to the field of analytical chemistry and electrochemistry, and particularly relates to an electrochemical sensor, a preparation method and application thereof in detection of sweat triglyceride content. A wearable sensor is prepared based on C-ZIF-67 / KB nanocomposite material, the sensor realizes detection of sweat triglyceride for the first time, and it is verified that sweat triglyceride level has high correlation with blood triglyceride level (correlation coefficient is 0.813, n=35). Through verification of a commercial colorimetric analysis kit, the sensor obtains a high correlation coefficient of 0.853 in detection of sweat triglyceride, which shows excellent accuracy.

The present invention relates to an anti-obesity composition comprising an extract of Rosa rugosa flower buds as an active ingredient, and by virtue of comprising the extract, aims to provide a composition having excellent anti-obesity efficacy by exhibiting the effects of inhibiting fat accumulation, reducing triglyceride content, increasing the expression of adipose triglyceride lipase (ATGL) and AMP-activated protein kinase (AMPK) proteins, and decreasing the expression of G0S2 (G0 / G1 switch 2) and perilipin proteins.

Disclosed is a process for preparing a final fat composition and its use in e.g. enrobing applications. The process involves the use of a first and a second vegetable fat composition with high contents of specific triglycerides, such as StOSt and POP, and the careful reduction of undesirable triglycerides like tri-palmitic and tri-stearic. The resulting fat composition is particularly suitable for use as a cocoa butter equivalent in chocolate and confectionery products, especially for enrobing applications. The process involves either blending these two fat compositions in specific weight ratios and then reducing the content of tri-palmitic and tri-stearic triglycerides to achieve a final fat composition with a certain percentage of POP+StOSt triglycerides, or individually reducing the tri-stearic triglyceride content in the first vegetable fat composition and the tri-palmitic triglyceride content in the second, followed by blending to obtain the final composition. The resulting fat composition is particularly suitable for various applications such as confectionery enrobing and coating, and as a cocoa butter equivalent.

The invention belongs to the technical field of biological medicines, and discloses a novel application of an epimedium aqueous extract, icariin and icaritin in improvement of senescence-related fat accumulation and redistribution. The invention constructs a nematode-mouse dual verification model: firstly, in a caenorhabditis elegans model, it is found that the active ingredients can significantly reduce fat droplet accumulation and triglyceride level in senescence nematode bodies, and especially under the concentration of 50 [mu] M, icariin can reduce the triglyceride content of 15-day-old senescence nematode to 37.58% of a control group. Furthermore, a naturally aged mouse is evaluated by utilizing a high-resolution Micro-CT (VivaCT 80) living body imaging technology, and three-dimensional reconstruction and quantitative analysis prove that the medicine can remarkably reduce the volume of visceral fat in the abdomen of the aged mouse, effectively improve the ratio of visceral fat to subcutaneous fat (VAT / SAT ratio) and reverse central obesity caused by aging. The raw materials are from natural plants, have no obvious toxic or side effect after long-term administration, can accurately improve fat space distribution of aging organisms, and have significant clinical application value.

To provide a powder cosmetic excellent in various characteristics of fragrance and feeling of use.SOLUTION: (A) when the total triglyceride content is 100% by mass, 80 to 99% by mass of one or more XXX triglycerides having fatty acids residues X with x carbon atoms at positions 1 to 3, and 20 to 1% by mass of one or more X2Y triglycerides in which one of the fatty acids residues X of the XXX triglyceride is substituted with fatty acids residues Y with y carbon atoms are contained; The number x of carbon atoms is an integer selected from 8 to 12, and the number y of carbon atoms is each independently an integer selected from x + 2 to x + 8. A powder cosmetic comprising a powdery fat and / or oil, (B) a powder component (excluding (A) the powdery fat and / or oil), (C) a liquid fat and / or oil incompatible with (A) the powdery fat and / or oil, and (D) a perfume, wherein the blending amount of (A) the powdery fat and / or oil is 8% by mass or more and 62% by mass or less relative to the total mass of the powder cosmetic.SELECTED DRAWING: None

The invention relates to an edible partially interesterified fat composition originating from a fraction of a shea butter, a sal butter or an illipe butter comprising: - an oleic acid content of at least 50% and a stearic acid content of at least 25%, by weight of the total weight of the fat composition, - a solid fat content of less than 10 at 10 °C and less than 7 at 20 °C, and - a StStSt triglyceride content of 1.9% by weight or less compared to the total weight of triglycerides in the fat composition, wherein St is stearic acid. The invention also relates to an edible spread or filling composition comprising the fat composition and to the use of the edible fat composition in a bakery, confectionary, chocolate, or chocolate-like spread or filling composition..

The invention discloses lactobacillus gasseri with a lipid-lowering function and application of the lactobacillus gasseri, and belongs to the technical field of functional probiotic screening and application. The lactobacillus gasseri with the lipid-lowering function is classified and named as lactobacillus gasseri LGI 6-1, and is preserved in the China General Microbiological Culture Collection Center on July 15, 2024, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.31285. The lactobacillus gasseri with the lipid-lowering function has the advantages that the lactobacillus gasseri with the lipid-lowering function can be used for preparing the lipid-lowering food; the lactobacillus gasseri is separated from excrement of healthy infants, can reduce lipid accumulation and the content of cholesterol and triglyceride, effectively prevents and relieves hyperlipidemia and obesity, has relatively strong tolerance to artificial gastrointestinal fluid, is sensitive to antibiotics such as tetracycline and vancomycin, cannot dissolve blood cells, has no toxic effect on organisms, and can be used for preventing and treating hyperlipidemia and obesity. The compound has good biological safety, can be added into functional food, special medical food, health care products or medicines, and has a wide application prospect.

The invention discloses polysaccharide for preventing and treating fatty liver hemorrhagic syndrome of laying hens and a preparation method of the polysaccharide, and belongs to the technical field of animal medicine. According to the method, the choline chloride-acetic acid eutectic solvent is used for ultrasonic-assisted extraction, so that the polysaccharide is mildly functionalized and degraded; then, through two-stage ultrafiltration of a 10 <-3 > kDa membrane, 3-10 kDa polysaccharide components are accurately collected. Experimental results show that the polysaccharide prepared by the specific process has an unexpected supernormal curative effect. In a high-fat-induced FLHS laying hen model, the polysaccharide can thoroughly reduce the overhigh triglyceride content of the liver to a level which is not obviously different from that of a healthy control group. Meanwhile, the liver bleeding score and the egg laying performance can be recovered to the normal level. Therefore, the invention provides a safe and effective feed additive for green and efficient prevention and treatment of FLHS.

The invention relates to application of obakunone in preparation of uric acid lowering drugs. The obakunone can be used for lowering serum uric acid level and liver tissue uric acid content. According to the experiment, a high-fat feed induced metabolism-related fatty liver disease mouse model is adopted, obakunone is administrated through intragastric administration, and the influence of obakunone on the uric acid level is observed; the result shows that the serum uric acid level and the liver tissue uric acid level of the obakunone intervention group are reduced. Meanwhile, obakunone can reduce the content of triglyceride in liver tissue and improve liver lipid deposition.

The present invention relates to a process for increasing the SOS triglyceride content of a vegetable oil, wherein S represents stearic acid (C18:0) and palmitic acid (C16:0) residues and O represents oleic acid (C18:1) residues, said process comprising: a) providing a reaction environment comprising: i) an sn-1,3-specific lipase immobilised on a support, ii) a vegetable oil, wherein the vegetable oil comprises at least 45% oleic acid fatty acid residues, based on total C6-C24 fatty acid residues, and wherein the vegetable oil has an oleic acid content in the sn-2 position of at least 75% by weight of the total sn-2 fatty acid residues of the vegetable oil, iii) an aliphatic alcohol ester of stearic acid, palmitic acid, or mixtures thereof, optionally mixed with stearic acid and / or palmitic acid, and iv) water; wherein the weight ratio of the aliphatic alcohol ester and optional stearic acid and / or palmitic acid (iii) to the vegetable oil (ii) is at least 4:1; and wherein the water activity of the reaction environment is in the range 0.1 to 0.6; b) heating the reaction environment to a temperature of 30-60° C. to perform transesterification, thus obtaining a mixture comprising a triglyceride phase, fatty acid esters, and optionally free fatty acids; and c) separating the fatty acid esters and free fatty acids from the mixture obtained in step (b) to obtain a triglyceride composition. The triglyceride phase may be used as a cocoa butter equivalent of a component thereof in chocolate or chocolate-like products.

This invention proposes an HPLC-ELSD method for determining the content of medium-chain triglycerides. Using tricaprylic acid glyceride and tricaprylic acid glyceride as controls, the method employs HPLC-ELSD based on a C18 column for separation, captures signals using an evaporative light scattering detector, and performs linear fitting using the logarithmic response values ​​and concentration values ​​of tricaprylic acid glyceride and tricaprylic acid glyceride. The logarithmic peak area of ​​the main peak of the test sample is substituted into the linear equation for calculation. The content of tricaprylic acid glyceride and dicaprylic acid-capric acid glyceride in the test sample is calculated using the regression equation of tricaprylic acid glyceride, and the content of tricaprylic acid glyceride and dicaprylic acid-capric acid glyceride in the test sample is calculated using the regression equation of tricaprylic acid glyceride. The total content of the four main triglycerides is calculated as the triglyceride content. This method overcomes the drawback of the difficulty in obtaining dicaprylic acid-capric acid glyceride and dicaprylic acid-capric acid glyceride controls, and offers high sensitivity and accuracy. It is simple, rapid, and suitable for industrial production and quality control.

The present invention addresses the problem of providing: an oil and fat composition for chocolates having excellent hardness, snapping properties, melt-in-the-mouth texture, lasting flavor (taste and aroma), and bloom resistance; and chocolates.　The oil and fat composition for chocolates contains oil-and-fat A that satisfies all of requirements (a1) to (a5) and oil-and-fat B that satisfies all of requirements (b1) to (b5).　(a1) The fatty acid content having a chain length of 14 carbon atoms or less is 40 to 70 wt% in the total constituent fatty acids of oil-and-fat A, (a2) the saturated fatty acid content having a chain length of 16 carbon atoms or more is 25 to 50 wt% in the total constituent fatty acids of oil-and-fat A, (a3) the unsaturated fatty acid content is 10 wt% or less in the total constituent fatty acids of oil-and-fat A, (a4) the weight ratio of the saturated fatty acid content / the unsaturated fatty acid content is 10 to 40 in the total constituent fatty acids of oil-and-fat A, (a5) the triglyceride content, in which the total carbon number of the constituent fatty acids in triglycerides is 40 to 44, is 30 to 50 wt% in the total triglycerides of oil-and-fat A, (b1) the fatty acid content having a chain length of 14 carbon atoms or less is 5 wt% or less in the total constituent fatty acids of oil-and-fat B, (b2) the saturated fatty acid content having a chain length of 16 carbon atoms or more is 20 to 65 wt% in the total constituent fatty acids of oil-and-fat B, (b3) ​​the unsaturated fatty acid content is 30 to 80 wt% in the total constituent fatty acids of oil-and-fat B, (b4) the weight ratio of the saturated fatty acid content / the unsaturated fatty acid content is 0.25 to 2 in the total constituent fatty acids of oil-and-fat B, and (b5) the weight ratio of the triglyceride content, in which the total carbon number of the constituent fatty acids in the triglycerides of oil-and-fat B is 50, to the triglyceride content, in which the total carbon number of the constituent fatty acids in the triglycerides of oil-and-fat B is 46, is 0.1 to 50.

The invention relates to the field of biological medicine, and particularly discloses application of natural plant component corilagin in prevention or treatment of metabolism-related fatty liver disease (MAFLD). Experiments prove that corilagin can significantly reduce the body weight and liver weight of MAFLD model mice, improve liver fatty degeneration and lipid deposition, reduce the levels of cholesterol, triglyceride, glutamic-pyruvic transaminase and glutamic oxalacetic transaminase in serum and liver tissues, and improve fasting blood glucose control and glucose metabolism capabilities. Mechanism research shows that the expression of fat synthesis related genes (Srebp1c, FASN and ACC1) is inhibited in a dose-dependent manner, and the expression of fat oxidation related genes (PPAR alpha, CPT1a and AMPK) is up-regulated, so that the regulation of lipid metabolism is realized. In-vitro experiments further prove that corilagin can inhibit lipid droplet accumulation in liver cells and reduce the content of triglyceride. The invention provides powerful technical support for development and application of corilagin in clinical treatment of MAFLD.