11 results about "Triglyceride content" patented technology

This invention relates to a method for rapidly screening filamentous fungi that produce high levels of triglycerides and polyunsaturated fatty acids, belonging to the field of microbial technology. The method employs a two-stage screening strategy of "resistance primary screening – fluorescence secondary screening." In the primary screening stage, strains with strong lipid synthesis capabilities are initially screened by adding a combination of phytocyanin and quizalofop-p-ethyl inhibitors to the culture medium. In the secondary screening stage, cellophane culture technology and Nile red fluorescence staining are combined to quantitatively assess the triglyceride content of the colonies, achieving rapid and non-destructive screening. This method is simple to operate, highly efficient, and shows a significant positive correlation between fluorescence intensity and triglyceride content. It is suitable for mutagenesis breeding and industrial strain selection of oil-producing filamentous fungi such as *Morchella alpineensis*.

The invention belongs to the technical field of microorganisms, and discloses clostridium butyricum ML5358 and application thereof.The clostridium butyricum ML5358 provided by the invention has the preservation number of CGMCC No.37000. The strain can effectively relieve mouse colitis induced by DSS and remarkably reduce expression of colon tissue inflammatory factors Il-6 and Il-1beta, and the preservation number of the clostridium butyricum ML5358 is CGMCC No.37000. The clostridium butyricum ML5358 is CGMCC No.37000. The clostridium butyricum ML5358 is CGMCC No.37000. In addition, skeletal muscle lipid metabolism can be regulated and controlled through the intestine-muscle axis, expression of fat synthesis and uptake related genes Srebf1, Acc1 and Fabp4 is specifically up-regulated, the content of intramuscular triglyceride is remarkably increased, and intramuscular fat deposition is promoted. According to the clostridium butyricum ML5358 and the application thereof, the functional limitation of traditional clostridium butyricum is broken through, and a new strain resource is provided for developing a novel microbial preparation or a feed additive capable of improving intestinal health and improving livestock and poultry meat quality.

The invention provides a processing method of peanut butter, and belongs to the technical field of food processing. Peanut kernels are degreased through a hydraulic oil pressing device, the content of triglyceride in the peanut kernels is reduced, and product energy is reduced; in order to make up flavor loss caused by reduction of the grease content, enzyme and protease are adopted to conduct enzymolysis on degreased peanut kernels, the hydrolytic activity of protein and saccharides is improved, the content of free amino acid and reducing sugar is increased, and a flavor precursor is provided for Maillard reaction in the subsequent heat treatment process; the heat treatment can promote the generation of aroma substances and enable the flavor to be more harmonious; in order to ensure the quality of the peanut butter and improve the fluidity of the peanut butter, the crushed peanuts after heat treatment and the light-fat peanut oil are mixed and ground, so that the light-fat peanut oil is uniformly dispersed. Compared with traditional peanut triglyceride, the light-fat peanut oil has a lower metabolic energy value and good interfacial activity, not only can improve the rheological property, lubricity and structural stability of the peanut butter, but also can promote release and retention of flavor active substances.

This invention discloses a method for evaluating the bioavailability of soybean oil based on an in vitro digestion / cell co-culture model. Soybean oil is emulsified using WPI emulsifier and then digested sequentially through simulated gastric and intestinal fluids to obtain the gastric digestion products of soybean oil. Fatty acids are extracted from the intestinal digestion products of soybean oil, dissolved in dimethyl sulfoxide, and linked to fatty acid-free bovine serum albumin to obtain in vitro digestion products of soybean oil that can be absorbed by the intestines. These are then added to a cell co-culture model for cell incubation and transport. HepG2 cells from the cell co-culture model are collected, and Oil Red O staining and total triglyceride content are measured to obtain lipid deposition, thereby evaluating the bioavailability of soybean oil. The in vitro digestion / cell co-culture model of this invention is specifically designed for soybean oil digestion. This method objectively and scientifically evaluates the bioavailability of soybean oil at different metabolic stages, providing a standard basis for the scientific and healthy consumption of soybean oil and possessing significant practical value.

The invention discloses a method for identifying or assisting in identifying the content of duck sebum triglyceride, a related composition and application, and belongs to the field of nucleic acid-containing determination methods. The method for identifying or assisting in identifying the content of the duck sebum triglyceride comprises the following steps: detecting the genotype of a specific SNP (Single Nucleotide Polymorphism) site of a duck to be detected, and identifying or assisting in identifying the content of the duck sebum triglyceride according to the genotype of the specific SNP site of the duck to be detected. The specific SNP site is an SNP site in a duck genome, the nucleotide type of the specific SNP site is T or C, and the specific SNP site is the 70th nucleotide of SEQ ID No.1. The method can be used for carrying out early selection on the multi-triglyceride content character of the duck sebum, so that the duck breeding efficiency is improved, and the breeding period is shortened.

The invention relates to the technical field of coreopsis tinctoria refined components and preparation and application, and provides a coreopsis tinctoria refined component and a preparation method and application thereof.The coreopsis tinctoria refined component comprises, by mass, 11 specific components of phenolic acids, flavonol, flavanol and flavonoid, and the 11 specific components comprise, by mass, 2 parts of gallic acid, 1 part of potassium sorbate, 1 part of potassium sorbate, 1 part of potassium sorbate and 1 part of potassium sorbate. The health-care food is prepared from the following raw materials in parts by weight: 3 parts of chlorogenic acid, 14 parts of quercetin, 18 parts of catechin, 5 parts of citric acid, 2-3 parts of procyanidine B, 3 parts of (+)-epicatechin, 1-31 parts of 31 parts of procyanidine C, 14 parts of rutin, 4 parts of camphanol-3-O-rutinoside and 2 parts of isorhamnetin-3-O-rutinoside. The snow chrysanthemum is Changbai Mountain snow chrysanthemum. Animal experiments show that the refined component can significantly reduce the content of total cholesterol and triglyceride in serum of a high-fat model rat, the specific component with blood fat regulating activity is prepared from Changbai Mountain snow chrysanthemum, and a new choice is provided for non-drug intervention of dyslipidemia.

This invention provides a method for quantitative analysis of intracellular lipid content based on the Bodipy 493 / 503 fluorescent probe, relating to the field of lipid metabolism detection technology. The method includes: (1) determining the absorption spectrum and excitation / emission spectrum of Bodipy 493 / 503 in isopropanol, and determining the maximum absorption wavelength and maximum excitation / emission wavelength of the fluorescent probe in isopropanol based on the spectral results; (2) staining the target cells with the Bodipy 493 / 503 probe after treatment with the compound, observing the changes in fluorescence signal in the field of view before and after isopropanol extraction, and taking pictures; (3) measuring the absorbance and fluorescence intensity of the isopropanol extract, analyzing the experimental results, and comparing them with the results of triglyceride content detection. This quantitative method is simple, fast, sensitive, effective, and has good experimental controllability and repeatability. The analytical results obtained are consistent with those obtained using other lipid content analysis methods, and it can be of great application in the field of research on the lipid metabolism interference effects of chemicals or environmental pollutants.

Disclosed is a process for preparing a final fat composition and its use in e.g. enrobing applications. The process involves the use of a first and a second vegetable fat composition with high contents of specific triglycerides, such as StOSt and POP, and the careful reduction of undesirable triglycerides like tri-palmitic and tri-stearic. The resulting fat composition is particularly suitable for use as a cocoa butter equivalent in chocolate and confectionery products, especially for enrobing applications. The process involves either blending these two fat compositions in specific weight ratios and then reducing the content of tri-palmitic and tri-stearic triglycerides to achieve a final fat composition with a certain percentage of POP+StOSt triglycerides, or individually reducing the tri-stearic triglyceride content in the first vegetable fat composition and the tri-palmitic triglyceride content in the second, followed by blending to obtain the final composition. The resulting fat composition is particularly suitable for various applications such as confectionery enrobing and coating, and as a cocoa butter equivalent.

The invention belongs to the technical field of biological medicines, and discloses a novel application of an epimedium aqueous extract, icariin and icaritin in improvement of senescence-related fat accumulation and redistribution. The invention constructs a nematode-mouse dual verification model: firstly, in a caenorhabditis elegans model, it is found that the active ingredients can significantly reduce fat droplet accumulation and triglyceride level in senescence nematode bodies, and especially under the concentration of 50 [mu] M, icariin can reduce the triglyceride content of 15-day-old senescence nematode to 37.58% of a control group. Furthermore, a naturally aged mouse is evaluated by utilizing a high-resolution Micro-CT (VivaCT 80) living body imaging technology, and three-dimensional reconstruction and quantitative analysis prove that the medicine can remarkably reduce the volume of visceral fat in the abdomen of the aged mouse, effectively improve the ratio of visceral fat to subcutaneous fat (VAT / SAT ratio) and reverse central obesity caused by aging. The raw materials are from natural plants, have no obvious toxic or side effect after long-term administration, can accurately improve fat space distribution of aging organisms, and have significant clinical application value.

This invention proposes an HPLC-ELSD method for determining the content of medium-chain triglycerides. Using tricaprylic acid glyceride and tricaprylic acid glyceride as controls, the method employs HPLC-ELSD based on a C18 column for separation, captures signals using an evaporative light scattering detector, and performs linear fitting using the logarithmic response values ​​and concentration values ​​of tricaprylic acid glyceride and tricaprylic acid glyceride. The logarithmic peak area of ​​the main peak of the test sample is substituted into the linear equation for calculation. The content of tricaprylic acid glyceride and dicaprylic acid-capric acid glyceride in the test sample is calculated using the regression equation of tricaprylic acid glyceride, and the content of tricaprylic acid glyceride and dicaprylic acid-capric acid glyceride in the test sample is calculated using the regression equation of tricaprylic acid glyceride. The total content of the four main triglycerides is calculated as the triglyceride content. This method overcomes the drawback of the difficulty in obtaining dicaprylic acid-capric acid glyceride and dicaprylic acid-capric acid glyceride controls, and offers high sensitivity and accuracy. It is simple, rapid, and suitable for industrial production and quality control.

The present invention addresses the problem of providing: an oil and fat composition for chocolates having excellent hardness, snapping properties, melt-in-the-mouth texture, lasting flavor (taste and aroma), and bloom resistance; and chocolates.　The oil and fat composition for chocolates contains oil-and-fat A that satisfies all of requirements (a1) to (a5) and oil-and-fat B that satisfies all of requirements (b1) to (b5).　(a1) The fatty acid content having a chain length of 14 carbon atoms or less is 40 to 70 wt% in the total constituent fatty acids of oil-and-fat A, (a2) the saturated fatty acid content having a chain length of 16 carbon atoms or more is 25 to 50 wt% in the total constituent fatty acids of oil-and-fat A, (a3) the unsaturated fatty acid content is 10 wt% or less in the total constituent fatty acids of oil-and-fat A, (a4) the weight ratio of the saturated fatty acid content / the unsaturated fatty acid content is 10 to 40 in the total constituent fatty acids of oil-and-fat A, (a5) the triglyceride content, in which the total carbon number of the constituent fatty acids in triglycerides is 40 to 44, is 30 to 50 wt% in the total triglycerides of oil-and-fat A, (b1) the fatty acid content having a chain length of 14 carbon atoms or less is 5 wt% or less in the total constituent fatty acids of oil-and-fat B, (b2) the saturated fatty acid content having a chain length of 16 carbon atoms or more is 20 to 65 wt% in the total constituent fatty acids of oil-and-fat B, (b3) ​​the unsaturated fatty acid content is 30 to 80 wt% in the total constituent fatty acids of oil-and-fat B, (b4) the weight ratio of the saturated fatty acid content / the unsaturated fatty acid content is 0.25 to 2 in the total constituent fatty acids of oil-and-fat B, and (b5) the weight ratio of the triglyceride content, in which the total carbon number of the constituent fatty acids in the triglycerides of oil-and-fat B is 50, to the triglyceride content, in which the total carbon number of the constituent fatty acids in the triglycerides of oil-and-fat B is 46, is 0.1 to 50.