4 results about "Triglyceride content" patented technology

This invention relates to a method for rapidly screening filamentous fungi that produce high levels of triglycerides and polyunsaturated fatty acids, belonging to the field of microbial technology. The method employs a two-stage screening strategy of "resistance primary screening – fluorescence secondary screening." In the primary screening stage, strains with strong lipid synthesis capabilities are initially screened by adding a combination of phytocyanin and quizalofop-p-ethyl inhibitors to the culture medium. In the secondary screening stage, cellophane culture technology and Nile red fluorescence staining are combined to quantitatively assess the triglyceride content of the colonies, achieving rapid and non-destructive screening. This method is simple to operate, highly efficient, and shows a significant positive correlation between fluorescence intensity and triglyceride content. It is suitable for mutagenesis breeding and industrial strain selection of oil-producing filamentous fungi such as *Morchella alpineensis*.

The invention relates to the technical field of coreopsis tinctoria refined components and preparation and application, and provides a coreopsis tinctoria refined component and a preparation method and application thereof.The coreopsis tinctoria refined component comprises, by mass, 11 specific components of phenolic acids, flavonol, flavanol and flavonoid, and the 11 specific components comprise, by mass, 2 parts of gallic acid, 1 part of potassium sorbate, 1 part of potassium sorbate, 1 part of potassium sorbate and 1 part of potassium sorbate. The health-care food is prepared from the following raw materials in parts by weight: 3 parts of chlorogenic acid, 14 parts of quercetin, 18 parts of catechin, 5 parts of citric acid, 2-3 parts of procyanidine B, 3 parts of (+)-epicatechin, 1-31 parts of 31 parts of procyanidine C, 14 parts of rutin, 4 parts of camphanol-3-O-rutinoside and 2 parts of isorhamnetin-3-O-rutinoside. The snow chrysanthemum is Changbai Mountain snow chrysanthemum. Animal experiments show that the refined component can significantly reduce the content of total cholesterol and triglyceride in serum of a high-fat model rat, the specific component with blood fat regulating activity is prepared from Changbai Mountain snow chrysanthemum, and a new choice is provided for non-drug intervention of dyslipidemia.

Disclosed is a process for preparing a final fat composition and its use in e.g. enrobing applications. The process involves the use of a first and a second vegetable fat composition with high contents of specific triglycerides, such as StOSt and POP, and the careful reduction of undesirable triglycerides like tri-palmitic and tri-stearic. The resulting fat composition is particularly suitable for use as a cocoa butter equivalent in chocolate and confectionery products, especially for enrobing applications. The process involves either blending these two fat compositions in specific weight ratios and then reducing the content of tri-palmitic and tri-stearic triglycerides to achieve a final fat composition with a certain percentage of POP+StOSt triglycerides, or individually reducing the tri-stearic triglyceride content in the first vegetable fat composition and the tri-palmitic triglyceride content in the second, followed by blending to obtain the final composition. The resulting fat composition is particularly suitable for various applications such as confectionery enrobing and coating, and as a cocoa butter equivalent.

This invention proposes an HPLC-ELSD method for determining the content of medium-chain triglycerides. Using tricaprylic acid glyceride and tricaprylic acid glyceride as controls, the method employs HPLC-ELSD based on a C18 column for separation, captures signals using an evaporative light scattering detector, and performs linear fitting using the logarithmic response values ​​and concentration values ​​of tricaprylic acid glyceride and tricaprylic acid glyceride. The logarithmic peak area of ​​the main peak of the test sample is substituted into the linear equation for calculation. The content of tricaprylic acid glyceride and dicaprylic acid-capric acid glyceride in the test sample is calculated using the regression equation of tricaprylic acid glyceride, and the content of tricaprylic acid glyceride and dicaprylic acid-capric acid glyceride in the test sample is calculated using the regression equation of tricaprylic acid glyceride. The total content of the four main triglycerides is calculated as the triglyceride content. This method overcomes the drawback of the difficulty in obtaining dicaprylic acid-capric acid glyceride and dicaprylic acid-capric acid glyceride controls, and offers high sensitivity and accuracy. It is simple, rapid, and suitable for industrial production and quality control.