Application of neohesperidin in hyperlipidemia prevention and treatment drug preparation

A technology for neohesperidin and hyperlipidemia, which is applied in the field of medicine and can solve the problems of no report on neohesperidin.

Inactive Publication Date: 2013-08-28
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The role of naringin and hesperidin in regulating glucose and lipid metabolism has been reported in the literature. In the study of extracting and separating components from the white cortex of citrus grapefruit, the inventors first discovered that neohesperidin has an in vitro hypoglycemic effect and carried out However, there is no report on the effect of neohesperidin on lipid metabolism

Method used

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  • Application of neohesperidin in hyperlipidemia prevention and treatment drug preparation
  • Application of neohesperidin in hyperlipidemia prevention and treatment drug preparation
  • Application of neohesperidin in hyperlipidemia prevention and treatment drug preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Neohesperidin has a lipid-lowering effect on free fatty acid-induced fat accumulation hepatocytes, and its effect is stronger than that of hesperidin and naringin. The experimental methods and results are as follows:

[0021] HepG2 cells were inoculated in a 5 ml culture dish with high-glucose DMEM medium containing 10% fetal bovine serum at 37 °C, 5% CO 2 Incubate in an incubator. When the cells are close to 70% confluent, wash them once with PBS, add part of 1640 culture medium, set up blank control group, solvent control group (free fatty acid solvent containing NaOH, the final concentration is 0.5 mmol / L), fatty acid Modeling model control group, lipid-lowering drug bezafibrate positive control group (sigma product, final concentration 10 μmol / L), neohesperidin group, hesperidin group and naringin group (sigma product, final concentration Both are 10 μmol / L). At 37°C, 5% CO 2 After 30 min in the incubator, 1640 culture solution (containing 0.5% BSA) with or ...

Embodiment 2

[0025] Neohesperidin has a lowering effect on triglyceride in hyperlipidemic C57 mice induced by egg yolk emulsion, and its effect is stronger than that of hesperidin and naringin. The experimental methods and results are as follows:

[0026] C57 mice were randomly divided into 6 groups, 6 in each group, respectively normal control group, model control group, bezafibrate positive control group, neohesperidin group, hesperidin group and naringin group, each given The drug group was intragastrically administered at a dose of 100 mg / kg. The normal control group and the model control group were given the same volume of solvent (0.5% CMC-Na). After 2 weeks of continuous administration, the animals were intraperitoneally injected with 75% fresh egg yolk emulsion. After 20 h, blood was collected to separate plasma, and triglyceride content was determined.

[0027] The results showed that after intraperitoneal injection of 75% fresh egg yolk emulsion in C57 mice, the plasma triglycer...

Embodiment 3

[0029] Neohesperidin has an effect on increasing high-density lipoprotein in hyperlipidemic C57 mice induced by egg yolk emulsion, and its effect is stronger than that of hesperidin and naringin. The experimental methods and results are as follows:

[0030] C57 mice were randomly divided into 6 groups, 6 in each group, respectively normal control group, model control group, bezafibrate positive control group, neohesperidin group, hesperidin group and naringin group, each given The drug group was intragastrically administered at a dose of 100 mg / kg. The normal control group and the model control group were given the same volume of solvent (0.5% CMC-Na). After 2 weeks of continuous administration, the animals were intraperitoneally injected with 75% fresh egg yolk emulsion. After 20 hours, blood was collected to separate plasma, and the content of high-density lipoprotein cholesterol (reflecting high-density lipoprotein) was measured.

[0031] The results showed that after intr...

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Abstract

The present invention provides an application of neohesperidin in hyperlipidemia prevention and treatment drug preparation, wherein the drug is prepared from single neohesperidin or a composition comprising the neohesperidin and naringin or metformin and a pharmaceutically acceptable dressing. With comparative researches on lipid regulation effects of the neohesperidin, the hesperidin and the naringin, results show that triglyceride content in a liver cell fat accumulation model can be reduced in vitro with the neohesperidin, a blood plasma triglyceride level of an egg yolk-induced acute hyperlipemia mouse model can be reduced and a blood plasma high density lipoprotein level can be increased in vivo with the neohesperidin, the effects provided by the neohesperidin are stronger than the effects provided by the hesperidin and the naringin, and combination of the neohesperidin and the naringin or the metformin provides a synergistic lipid lowering effect. In addition, neohesperidin-containing plant extracts or neohesperidin-containing compound drugs or health products can be prepared so as to treat or prevent hyperlipidemia.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to the application of neohesperidin, a flavonoid compound from natural sources, or its composition in the prevention and treatment of hyperlipidemia, in particular to the application of neohesperidin in the preparation of single-purpose medicine or compound prescription for preventing and treating hyperlipidemia Application in medicine or health products. Background technique [0002] Hyperlipidemia refers to high levels of total cholesterol and / or triglycerides in the blood, accompanied by high LDL cholesterol or low HDL cholesterol (which has the effect of clearing cholesterol in the body). Modern medicine calls it Dyslipidemia, commonly known as "hyperlipidemia". Dyslipidemia is very common in middle-aged and elderly people. It is the pathological basis of atherosclerosis, and often has a causal relationship with metabolic syndromes such as diabetes, insulin resistance, obesity, non-alcoh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P3/06A23L1/30A61K31/155
Inventor 吴昊姝杨波何俏军陈昆松李鲜孙崇德
Owner ZHEJIANG UNIV
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