Applications of pomfret oil or fractionation extract thereof in preparation of human milk fat substitutes

A technology to replace fat and pomfret, applied in the fields of application, edible oil/fat, dairy products, etc., can solve the problems of low milk fat similarity, insufficient OPL, expensive enzymatic synthesis, etc., achieve high similarity and improve absorption The effect of using and reducing the occurrence of constipation and intestinal diseases

Inactive Publication Date: 2020-09-25
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When only black fish oil or pangasius oil is used for formulation, the problem of insufficient OPL in infant milk powder oil cannot be solved, and the similarity with breast milk fat is low
In addition, there are few studies on the synthesis of OPL by enzymatic methods, and the enzymatic synthesis is more expensive

Method used

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  • Applications of pomfret oil or fractionation extract thereof in preparation of human milk fat substitutes
  • Applications of pomfret oil or fractionation extract thereof in preparation of human milk fat substitutes
  • Applications of pomfret oil or fractionation extract thereof in preparation of human milk fat substitutes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Golden Pomfret Oil

[0039] The golden pomfret oil was extracted by chloroform-methanol method, and the fresh golden pomfret liver was freeze-dried and crushed. Take 10 g of crushed powder, add 100 mL of methanol, shake, then add 200 mL of chloroform, ultrasonicate for 20 minutes, take clear liquid after suction filtration, add 100 mL of sodium chloride solution with a mass fraction of 0.88%, and centrifuge the mixed solution at 4500 rpm After 10 minutes, absorb the lower layer, place it in a round bottom flask, and remove the solvent by rotary evaporation to obtain golden pomfret oil. The contents of palmitic acid, sn-2-position palmitic acid, OPO and OPL in gold pomfret oil were 23.52%, 37.25%, 15.84% and 25.67%, respectively.

Embodiment 2

[0040] Embodiment 2: freshwater white pomfret oil

[0041] With reference to the chloroform-methanol method of Example 1, freshwater pomfret oil was extracted. The contents of palmitic acid, sn-2 palmitic acid, OPO and OPL in freshwater pomfret oil were 25.26%, 36.96%, 26.39% and 19.38%, respectively. The content of OPL is slightly lower than that of golden pomfret oil, and a small amount of OPL structural fat can be added to formulate human milk substitute fat.

Embodiment 3

[0042] Embodiment 3: a kind of preparation method of the fractionation product of gold pomfret oil

[0043] The golden pomfret oil obtained in Example 1 was fractionated by solvent method, the solvent used was acetone, the ratio of golden pomfret oil to solvent (w / v) was 1:8, the fractionation temperature was -6°C, and the fractionation time was 12h, to obtain Golden Pomfret Oil Fraction. The contents of palmitic acid, sn-2 palmitic acid, OPO and OPL in the golden pomfret oil fraction were 27.82%, 45.37%, 20.28% and 29.46%, respectively.

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Abstract

The invention discloses applications of pomfret oil or fractionation extract thereof in preparation of human milk fat substitutes, and belongs to the technical field of food. Little UPU type triglyceride exists in natural oil; and few natural oil is rich in OPO but low in OPL content. The highest content of triglyceride in golden pomfret oil is OPL, followed by OPO; and the triglyceride content ofthe golden pomfret oil is similar with the triglyceride composition of Chinese breast milk fat. The golden pomfret oil, Colossoma brachypomum or fractionation extract thereof are selected as base oilfrom the pomfret oil; and a human milk fat substitute can be obtained by adding other natural oil or modified oil through compounding. The similarity of human milk fat substitute and breast milk fatis high, so that the problems of low absorptivity of infant formula milk powder fatty acid and calcium can be solved; the absorption and utilization of energy can be enhanced; the hardness of feces can be improved; and the occurrence of constipation and intestinal diseases in infants and young children can be reduced.

Description

technical field [0001] The invention relates to the application of pomfret oil or its fractions in the preparation of human milk substitute fat, belonging to the technical field of food. Background technique [0002] Breast milk is the best food for infants and young children, it can provide all the nutrients needed for growth and development of infants and young children. The lipid component in breast milk is called human milk fat, accounting for about 3% to 5%, providing 40% to 50% of energy and essential fatty acids for infants and young children, and can promote the absorption of fat-soluble vitamins. Human milk fat is a complex oil mixture, and most triglycerides in human milk fat exist in the form of sn-UPU, such as 1,3-dioleic acid-2-palmitic acid triglyceride (OPO) and 1-oleic acid-2 - palmitic acid-3-oleic acid triglyceride (OPL), etc. The unique distribution of fatty acids on the triglyceride backbone affects digestion, absorption and metabolism in infants. Nume...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23D9/04A23C9/152
CPCA23D9/04A23C9/1528
Inventor 王小三程昕祎杨壮壮江聪黄健花金青哲王兴国
Owner JIANGNAN UNIV
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