The invention relates to the technical field of preparation of an animal feed, in particular to a feed for lactation sows and a preparation method of the feed. The feed comprises corn, soybean oil, soybean meal, puffed soybeans, brown sugar, glucose, fermented soybean meal, fish meal, dicalcium phosphate, stone powder, salt, sodium bicarbonate, choline chloride, lysine, methionine, phytase, molasses, a premix feed and feed raw materials rich in soluble fibers. The raw materials mutually cooperate and jointly act under specific composition. In the feed, the brown sugar, the glucose, the molasses, the corn and the soybean oil are used as energy feeds; the soybean meal and the fish meal are main sources of the lysine; and the fish meal, the dicalcium phosphate, the phytase, the salt, the stone powder and the premix feed provide calcium, phosphorus, sodium, vitamins and trace elements. In the feed, the raw materials achieve the effects of increasing the food consumption of the lactation sows, reducing the constipation possibility of the lactation sows, shortening the time interval of the lactation sows from weaning to oestrus, increasing the weaned survival rate of piglets, and increasing the weight of the weaned piglets.