4 results about "Alloxan" patented technology

The invention provides an analysis method for relieving diabetic foot wound scars through tibia transverse movement, and belongs to the technical field of postoperative analys.The method comprises the steps that a New Zealand rabbit serves as an experimental subject, a diabetic rabbit model is built through alloxan, and a DFU wound model is built through femoral artery ligation; bone transport and VEGF (vascular endothelial growth factor) are used for intervention, and the following conclusions are obtained by observing changes of general conditions, pathological changes of skin tissues, inflammatory factors of skin tissues of New Zealand rabbits and expression levels of related genes and proteins in TGF-beta1 / Smad3 signaling pathways: 1, bone transport can inhibit expression levels of inflammatory factors IL-6, IL-18 and TNF-alpha; therefore, the infiltration degree of inflammatory cells in skin tissues can be reduced, and local inflammation and scarring can be effectively improved. 2, the bone transport can promote the expression of related protein on a TGF-beta1 / Smad3 signal channel, so that the effects of promoting muscle fiber contraction, revascularization and reducing scar generation are achieved;

The invention provides a method for preparing chiral sulfinic acid thioester through asymmetric oxidation, which is characterized in that a disulfide compound is used as a raw material, a chiral aminoindanol-derived alloxan compound is used as a chiral catalyst, hydrogen peroxide or oxygen is used as an oxidant, and the chiral sulfinic acid thioester is prepared through asymmetric oxidation. Compared with a traditional chiral imine ligand matched with a vanadyl acetylacetonate catalyst, an aminoindanol-derived alloxan compound is used as a chiral small-molecule catalyst, so that the chiral small-molecule catalyst has higher catalytic stability and high optical purity of a product, is not influenced by factors such as the stirring speed, the shape and size of a reaction bottle and the like, and is convenient for engineering amplification; meanwhile, compared with a chiral resolution method, the method for synthesizing the chiral sulfinic acid thioester in one step by using a chiral small molecule catalysis method has higher step economy, and the method is simple to operate, high in raw material conversion rate, good in reaction enantioselectivity, high in product yield and high in optical purity.

The application discloses a kind of construction methods of diabetic nephropathy combined with acute kidney injury rabbit comorbidity model, it is related to biological medicine technical field, the construction method includes the following steps: rabbit is fed with high-fat high-sugar feed, then injection tetraoxypyrimidine, constructs type 2 diabetes rabbit model, continue to feed high-fat high-sugar feed, detect rabbit blood sugar, blood fat and urine albumin / creatinine ratio, carries out pathological examination of kidney tissue, determines whether the diabetic nephropathy rabbit model is established successfully, to the diabetic nephropathy rabbit model that is successfully constructed injection folic acid-sodium bicarbonate solution, detect blood urea and creatinine level, carry out pathological examination of kidney tissue, determine whether the diabetic nephropathy combined with acute kidney injury rabbit comorbidity model is successfully constructed.The application provides effective animal model for the pathogenesis and new treatment target of diabetic comorbidity, effectively solves the problem that single animal model cannot comprehensively reflect complex disease and the influence of drug side effects on experimental animals.

The invention relates to the technical field of functional food microorganisms, in particular to lactobacillus plantarum LPL28 and application thereof. According to the invention, a diabetes model is established by adopting high-fat feed and alloxan, and the lactobacillus plantarum LPL28 strain is continuously given to a mouse through intragastric administration for 21 days, so that the treatment effect of the lactobacillus plantarum LPL28 strain on the diabetes model mouse is discussed. Research results show that the lactobacillus plantarum LPL28 strain effectively regulates intestinal flora through probiotics, and the blood sugar reducing effect of the lactobacillus plantarum LPL28 strain is exerted by fully exerting the efficacy of the flora. In addition, the lactobacillus plantarum LPL28 or a microbial agent taking the lactobacillus plantarum LPL28 as an active ingredient is applied to preparation of products for preventing and / or treating diabetic complications, the preservation number of the lactobacillus plantarum LPL28 is CGMCC17954, and the complications comprise at least one of the following complications: (1) hepatomegaly and / or injury; (2) treating hyperlipidemia; and (3) oxidative stress reaction.