73 results about "Alloxan" patented technology

The invention relates to a blood sugar level or blood pressure regulating liquid composite based on Noni fermented juice and preparation method thereof, belonging to health-care food and preparation method thereof. The invention prepares Noni normal juice by fermentation, appropriate flavouring agent and preservative are added, and pasteurization, cooling and filtering are carried out, thus obtaining the liquid composite. The invention takes animal experiment result of Noni juice influence on diabetes mice blood sugar level and normal rat blood pressure caused by alloxan as basis, and the prepared product has unique taste, is clear, can reduce blood sugar level or blood pressure and has health care efficacy.

The invention relates to an application of a black raspberry extract in preparing an anti-diabetic medicine, and an application of the black raspberry extract in preventing type-II diabetes mainly characterized by glucose metabolic disorders, beta islet cell damage and insulin resistance. A novel anti-diabetic medicine preparation provided by the invention takes the black raspberry extract as an active ingredient, and the total effective dose is 1-100mg/kg/d. According to the novel anti-diabetic medicine preparation provided by the invention, the black raspberry extract can be compounded with the diabetes treatment medicines on the current market including sulfonylureas, biguanides or thiazolidinediones to prepare a compound preparation. The black raspberry extract can promote islet cell multiplication and inhibit the PTP1B activity; and meanwhile, in-vivo study finds that the black raspberry extract can be used for improving the increase of the mice blood glucose level caused by alloxan and has an obvious effect in reducing blood glucose. Through verification, the blood glucose reducing effect of the black raspberry fruit or leaf extract is superior to that of the selected positive medicine diaformin.

The invention relates to a euonymus alatus extract and a preparation process, a quality control method, the blood-sugar-reducing activity of the euonymus alatus extract and application of the euonymus alatus extract to the preparation of products for reducing blood sugar. The euonymus alatus extract can be prepared by any one of a solvent extracting method, a macroporous adsorbent resin method, a supercritical fluid extraction method, column chromatography, liquid-liquid counter-current partition chromatography and the like or a combination of the methods. The prepared euonymus alatus extract comprises the following active ingredients: flavonoids, phenols, alkaloids, triterpenoids, sterides, organic acids and polysaccharides, wherein the flavonoids and the phenols are main ingredients, and the content of general flavones is between 5 and 100 weight percent. Blood-sugar-reducing experiments of mice which suffer from diabetes caused by mesoxyalyurea prove that the raising of the blood sugar after the intragastric administration of glucose is inhibited effectively, and the euonymus alatus extract has a certain treatment effect on experimental diabetes caused by pancreas islet beta cell injury caused by the mesoxyalyurea, and can be applied to the preparation of the products for reducing the blood sugar.

The invention discloses a use of ocean collagen peptide used in preparation of blood-glucose medicine, hypoglycemic health food or hypoglycemic food, which is characterized by high absorption, fast absorption, low viscosity, and good water-solubility etc. Experiment proves that the ocean collagen peptide given to a little mouse can lower alloxan hyperglycosemia of fasting blood-glucose level of a big mouse. Meanwhile, sugar tolerance of a hyperglycemia big mouse is improved, which indicates that the ocean collagen peptide has hypoglycemic action.

The invention discloses a preparation method of mollugin. The preparation method includes: taking India madder root, and using 5-15 times of petroleum ether to perform heating reflux extraction for 20-40min; recycling petroleum ether, and vacuum drying to remove petroleum ether so as to obtain extract; and subjecting the extract to 200-300-mesh silica gel column chromatography, eluting with petroleum ether, performing TLC (thin layer chromatography) detection, combining components rich in mollugin, subjecting to Sephadex LH-20 gel column chromatography, eluting with mixture of chloroform, petroleum ether, and methanol in mass ratio of 5:5:1, subjecting to silica gel H-column decompression column chromatography, eluting with petroleum ether, and recrystalizing with hot ethanol to obtain the mollugin. The invention further discloses application of the mollugin in preparation of hypoglycemic drugs and preparation of drugs for treating diabetes. Experiments show that the mollugin can effectively reduce blood sugar, TC (total cholesterol), TG (total glycerin) and MDA (methylene dioxyamphetamine) content of mice with high blood sugar caused by alloxan, and can evidently increase SOD (superoxide dismutase) activity and hepatic glycogen content of the mice.

The invention provides a guava hypoglycemic active component, and a preparation method and use thereof. The method comprises the following steps: crushing a raw material of guava leaves; carrying out reflux extraction by 8-10fold by volume of 50% ethanol; carrying out vacuum concentration below 60 DEG C; dispersing the obtained ethanol extract into water; fully extracting by adopting ethyl acetate after petroleum ether is extracted; carrying out vacuum concentration below 60 DEG C; filtering the obtained ethyl acetate extract by a 60-200mesh polyamide chromatographic column; eluting ethanol water from low to high in a gradient manner; collecting a component which is eluted by ethanol of which the concentration is 30-50%, continuing to filter by the 60-200mesh polyamide chromatographic column after vacuum concentration below 60 DEG C; carrying out isocratic elution by 30% ethanol after eluting by 10% ethanol; carrying out vacuum concentration on eluent below 60 DEG C, and recrystallizing by a 70% ethanol solvent, so as to obtain the hypoglycemic active component. The method disclosed by the invention is simple and convenient; chemical components of an effective part are clear in composition, and good in chemical stability. By adopting the guava hypoglycemic active component disclosed by the invention, alloxan-induced hyperglycemia can be significantly reduced, and the guava hypoglycemic active component can be applied to preparation of hypoglycemic medicaments or health-care products.

The invention relates to the activity of golden camellia tea for assisting in lowering blood sugar, the capsule of golden camellia for assisting in lowering blood sugar and a preparation method thereof. The prescription of golden camellia tea auxiliary hypoglycemic capsule is: 33%-66% of golden camellia tea extract, 67%-34% of medicinal excipients. The preparation method is as follows: taking golden camellia tea extract and medicinal auxiliary materials according to the weight percentage of the prescription, and processing them into hard capsules. Among them, golden camellia tea extract refers to the extract obtained by soaking, extracting, and concentrating the golden camellia tea leaves with an appropriate solvent; pharmaceutical excipients refer to medicinal starch or dextrin or a mixture of the two; solvent refers to water or ethanol or a mixture of both. Jinhua tea capsules have a significant hypoglycemic effect on mice with hyperglycemia induced by alloxan. The golden camellia tea capsule of the present invention and its function of assisting in lowering blood sugar. There is no report before this, and this is found by the inventor of this patent through experiments, therefore, special application for patent protection.

The invention provides application and blood-sugar lowering effective part of trapa acornis nakano shell as well as an extraction method of the blood-sugar lowering effective part, belonging to the technical field of traditional Chinese medicine. The new application of the trapa acornis nakano shell is to prepare a blood-sugar lowering medicine. The blood-sugar lowering effective part of the trapa acornis nakano shell is selected from one or any composite of petroleum ether part (TAPE), ethyl acetate part (TAEA) and n-butyl alcohol part (TABU) of the alcohol extract of trapa acornis nakano shell. The extraction method of the effective part comprises the following steps: grinding the dried trapa acornis nakano shell, carrying out digestion on the trapa acornis nakano shell powder with the aqueous solution of methyl alcohol or ethanol, and concentrating the immersion liquid to obtain extract; extracting the extract with petroleum ether, ethyl acetate or n-butyl alcohol for three times respectively; and volatilizing the dissolvent to obtain the TAPE, the TAEA or the TABU of the trapa acornis Nakai shell. Each effective part has good activity in inhibiting alpha-glucuroide. In vitro tests prove that TAEA and TABU have relatively good therapeutic effect on mice suffering from diabetic induced by mesoxyalyurea.

The invention discloses a Chinese medicinal composition for treating diabetes mellitus and a preparation thereof. The Chinese medicinal composition is prepared to form a certain preparation in the following steps: extracting and separating mulberry twig, mulberry leaf, dogwood, rhizoma anemarrhenae, prepared rehmannia and golden thread with 0 to 75 percent ethanol. The six medicaments are combined, so that the Chinese medicinal composition has the functions of tonifying kidney and consolidating vital energy, nourishing yin and gathering essence, clearing heat and purging intense heat, and reducing blood sugar and quenching thirst, and is used for treating light and moderate diabetes mellitus. Pharmacodynamic researches prove that: the medicinal composition can remarkably reduce the high blood sugar value of a streptozotocin and alloxan induced diabetes mellitus experimental diabetic model, and can remarkably reduce the sugar tolerance of a mice.

The invention discloses a grifola frondosa health care oral solution and a preparation method thereof. The preparation method comprises the following steps: taking a grifola frondosa fungus as an original strain and obtaining a grifola frondosa liquid strain; inoculating the grifola frondosa liquid strain to a solid culture-medium, prepared by wild herbage including miscanthus floridulus, jujun grass, pennisetum purpureum, dicranopteris linearis and the like according to specific proportion, , performing solid fermentation for 25-40 days, taking out a fermentation hyphostroma product, obtaining an extracting solution rich in grifola frondosa polysaccharide through the fermentation hyphostroma product in a hot water leaching method, and making the extracting solution into a concentrate; mixing the concentrate and Chinese medicine liquid prepared by decocting traditional blood sugar reduction Chinese medicines according to a mass ratio of 1:(0.5-1) to prepare the oral solution with a good taste. According to the invention, the content of the grifola frondosa polysaccharide obtained via the solid fermentation hyphostroma can reach 3.05 percent; the product is subjected to an animal blood sugar reduction test, the test shows that the grifola frondosa health care oral solution has an effect of reducing I-type high blood sugar model mouse blood sugar caused by Alloxan, and the blood sugar reduction ratio can reach 41.10 percent; in addition, the preparation method is stable and applicable to industrial production.

The invention relates to an active part of lysimachia trientaloides Hemsl.. The active part of the lysimachia trientaloides Hemsl. may be one or any combination of a petroleum ether part, an ethyl acetate part or an n-butyl alcohol part of the lysimachia trientaloides Hemsl. extracts. The extraction method particularly comprises the following steps of: lixiviating dry lysimachia trientaloides Hemsl. powder with methanol or ethanol aqueous solution; concentrating the immersion liquid to obtain an extractum; dispersing the extractum into distilled water; extracting by using the solvents of petroleum ether, ethyl acetate part and n-butyl alcohol sequentially for three times respectively; and volatilizing the solvents to obtain the petroleum ether part, the ethyl acetate part and the n-butyl alcohol part respectively. The active part of the lysimachia trientaloides Hemsl. has high inhibition activity on alpha-glucosidase and good curative effect on the mesoxyalyurea induced diabetes mice,and can be used for preparing medicines for reducing blood sugar.

The invention discloses a pharmaceutical composition for resisting diabetes activity and a preparation method thereof. The composition is composed of an olea europaea leaf extract and a carrier, wherein the carrier is selected from starch, beta-cyclodextrin, a talcum powder, microcrystalline cellulose and magnesium stearate. In-vitro cell glucose consumption experiments prove that the composition increases the glucose consumption amount of model cells and has obvious hypoglycemic effect. In-vivo animal experiments prove that the composition has a significant role in lowering blood glucose on mesoxyalyurea induced hyperglycemic mice.

The invention relates to application of a Mongolian medicine Cymbaria n-butanol extract in the preparation of medicine for preventing and curing diabetes, and belongs to the field of medicine. The invention also discloses the application of the Mongolian medicine Cymbaria n-butanol extract in the preparation of medicine for preventing and curing diabetes, a preparation method of the Cymbaria n-butanol extract, and a medicine composition consisting of various carriers with acceptable active ingredients, excipients or diluents contained in the Cymbaria n-butanol extract. The research of pharmacological experiments shows that the Cymbaria n-butanol extract with the dose of 250 mg/kg has an effect of reducing the blood sugar of a mice suffering from diabetics induced by alloxan, has a higher hypoglycemic effect compared with a normal comparison group, and has a similar blood sugar reduction pharmacologic action as compared with a positive drug metformin hydrochloride group. The result of experiment research provides powerful experiment support for reasonable application of Cymbaria Mongolian medicinal materials.

The invention provides a preparation method of assistant hypoglycemic corn-stigma extractive capsules. The active ingredient of the corn stigma extractive capsules is corn-stigma total flavones. A corn-stigma extractive is obtained through extraction by means of an ethyl alcohol organic solvent, and the marking ingredient of the corn-stigma extractive is the total flavones. An optimal process is obtained through an orthogonal experiment, and the content of the corn-stigma total flavones is high. A process for preparing the corn-stigma extractive capsules is optimized. The corn-stigma extractive capsules pass an assistant hypoglycemic function test, and the high-dosage corn-stigma extractive can remarkably inhabit continuous blood glucose rise (p<0.05) of a mouse model suffering from diabetes caused by alloxan. The corn-stigma extractive capsules belong to a newly-developed pure natural assistant hypoglycemic health-care food which is remarkable in hypoglycemic effect, free of toxic and side effects, safe and effective and can be taken for a long time.

The invention discloses an acrylic acid modified waterborne polyurethane self-repairing pressure-sensitive adhesive as well as a preparation method and application thereof. The invention provides a preparation method of an acrylic acid modified waterborne polyurethane self-repairing pressure-sensitive adhesive, which comprises the following steps: stirring polymer diol, hydrophilic carboxylic aciddiol, diisocyanate, alloxan monohydrate, an antioxidant, a catalyst and an organic solvent to react, thereby generating an isocyanate-terminated first prepolymer; adding 2,4,6-triaminopyrimidine anda cross-linking agent into the first prepolymer to obtain a second prepolymer; then adding acrylic acid small molecules and acrylic acid oligomers, carrying out a reaction, then adding a neutralizer to react to form salt, and then adding an aqueous solution containing a small molecule chain extender to obtain an emulsion; and adding an alkene monomer initiator, and carrying out a reaction to obtain the acrylic acid modified waterborne polyurethane self-repairing pressure-sensitive adhesive. The high-strength toughness self-repairing waterborne polyurethane pressure-sensitive adhesive providedby the invention has good mechanical properties, self-repairing performance, thermal stability and high temperature resistance.

The invention relates to application of turnip total saponins extracted from turnip plants to preparing a hypoglycemic medicament. The turnip total saponins can be used for lowering blood sugar of an alloxan-induced diabetic mouse and simultaneously improving pathological changes of visceral organs; meanwhile, related tests of pharmacological effectiveness are performed on animal hyperglycemia caused by adrenalin; and a result shows that the medicament has an obvious effect on lowering blood sugar.

The invention discloses a method for constructing an animal model of skin ulcer with diabetes. A Kunming white mouse is chosen and the abdominal cavity is injected with alloxan reagent, the mid lower portion of the back is unhaired, sterilized and cut deep to be under the skin, then the white mouse is fed in a single case and fixed amount of water and food is supplied. After fast for 12 hours, blood is taken from the tail of the white mouse and the blood sugar value is tested by a blood sugar tester to be properly more than or equal to 10.0mmol/L. The statistical analysis of body surface wound observation and the histological wound observation of the experimental animal model proves that the white mouse model of skin ulcer with diabetes that is constructed by alloxan is feasible and can be taken as the study model of pathogenesis and drug effect mechanism of skin ulcer with diabetes. Compared with the prior art, the white mouse model of skin ulcer with diabetes constructed by the invention has the advantages of simple model constructing method, high model forming ratio, low cost, short cycle and good reproducibility, etc.

The invention discloses a blood-glucose-reducing antioxidative granule based on wild guava fruit extract and a preparation method thereof. The granule comprises, by weight, 2 parts of the wild guava fruit extract, 1 to 2.5 parts of a filler and 0.3 to 0.8 part of a binder. After gavage with the granule for 40 d, fasting blood glucose of mice with alloxan-induced diabetes returns to a value below the normal value 7 mmol/L, and activity of superoxide dismutase in serum of the mice is improved. The granule is prepared through ultrasonic treatment, aided warm water extraction of surface active substances, preparation of blood-glucose-reducing antioxidative active extract and controlling reasonable parameters. The granule further comprises abundant protein and polysaccharide and can prevent hypoglycemic response. Moreover, the granule has the advantages of high efficiency, small usage amount, capacity of overcoming the problems of low utilization rate and poor efficacy of dried guava slice tea and multiple functions like blood glucose reduction, antioxidation and nutritional support, and provides a novel option for adjunctive treatment of patients with diabetes and for nutrition and health.

The invention discloses an application of two phenylethanoid glycosides in preparation of anti-diabetic drugs, and relates to the application of the two phenylethanoid glycosides verbascoside and isoacteoside in the preparation of the drugs for protecting and repairing islet beta cell damage, especially in the preparation of the anti-diabetic drugs. The verbascoside and isoacteoside monomer compounds can be extracted from a plurality of plants, and have the advantages of a wide range of sources, low toxicity and good stability. Through experiments, the inventors verify that the verbascoside and isoacteoside can protect and repair the NIT-1 islet beta cell damage caused by alloxan, and have a remarkable effect. Therefore, the two phenylethanoid glycosides verbascoside and isoacteoside provided by the invention have the potential value to be developed into the anti-diabetic candidate drugs.

The invention discloses a method for chemically synthesizing a mangiferin hexa-butyl-esterified derivative and a pharmacological effect of resisting inflammation and reducing blood sugar of the derivative. The chemical structural formula of the derivative is shown in the specifications. The derivative has a remarkable effect of inhibiting mouse auricle tumefaction caused by dimethylbenzene; and the derivative has a remarkable effect of treating diabetic mice induced by alloxan.

The invention discloses a method for constructing a diabetes mellitus animal model, which comprises the following steps: an alloxan reagent is injected in the abdominal cavity of a selected Kunming white mouse; then the white mouse is fed in a cage independently; water and food are fed to the white mouse quantitatively; after the white mouse is forbidden to eat food for 12 hours, blood is taken from the tail of the white mouse; a blood sugar meter is used for detecting a blood sugar value, and when the blood sugar value is larger than or equal to 10.0 mmol/L, the steps are ended. Proved by a statistical analysis of a blood sugar measuring result of the diabetes mellitus animal model, the method for constructing a white mouse diabetes mellitus model by utilizing the alloxan is feasible, and the model can be used as a research model of the pathogenesis and the drug mechanism of diabetes mellitus. Moreover, compared with the prior art, the constructed while mouse diabetes mellitus model has the advantages of simple model constructing method, high model forming rate, low cost, short period, good reproducibility, and the like.