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Preparation method and applications of auricularia polytricha blood fat reduction function protein extract

A technology of Auricularia auricularia protein and extract, which is applied in the direction of peptide preparation, peptide/protein composition, application, etc., to achieve the effect of alleviating hyperlipidemia, lowering triglyceride content, and lowering low-density lipoprotein cholesterol content

Active Publication Date: 2013-07-24
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, studies have shown that there are about 70 kinds of single Chinese medicines with lipid-lowering effects, but there are relatively few materials derived from edible fungi

Method used

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  • Preparation method and applications of auricularia polytricha blood fat reduction function protein extract
  • Preparation method and applications of auricularia polytricha blood fat reduction function protein extract
  • Preparation method and applications of auricularia polytricha blood fat reduction function protein extract

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Embodiment 1

[0032] Embodiment 1, the preparation of Auricularia officinalis protein extract

[0033] 1. Prepare the fruiting body of Auricularia auricula

[0034] Inoculate Auricularia polytricha (Mont.) Sacc. No. 3 slant strains into the first-level medium for activation, culture at a constant temperature of 25°C, and inoculate it into the second-level medium after the mycelium is full of the test tube in a constant temperature culture room at 25°C until the hyphae are overgrown, inoculate the second-grade species into a cultivation bag with a cultivation medium for growth at 25°C, and scratch the bacteria after the strain is full of the cultivation bag and transplant it into the In the greenhouse, the fruiting conditions should keep the humidity above 90%, and the temperature is about 20-25°C. Collect the first tide fruiting bodies to obtain No. 3 fruiting bodies of Auricularia polytricha (Mont.) Sacc.

[0035] Wherein, the culture medium used in this experiment is as follows:

[0036...

Embodiment 2

[0044] Embodiment 2, Auricularia auricularia protein extract lowering blood lipid experiment

[0045] 1. Preparation of blood lipid-lowering drugs

[0046] The dry powder of the protein extract of Auricularia trichomonas prepared in Example 1 was dissolved with deionized water to obtain an aqueous solution of the protein extract of Auricularia trichocarpa with a protein content of 6.67 mg / ml as a blood lipid-lowering drug.

[0047] Wherein, the assay method of the protein content of Auricularia auricularia protein extract aqueous solution is as follows:

[0048] 1) Preparation of standard curve: Use the standard sample bovine serum albumin (BSA) to prepare protein solutions with different concentrations, and use BCA (Beijing Biomed Technology Co., Ltd.) protein quantification kit to prepare the standard curve.

[0049] 2) The BCA protein quantification kit was used to determine the protein content in the aqueous solution of the protein extract of Auricularia auricula.

[005...

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Abstract

The present invention discloses a preparation method and applications of an auricularia polytricha blood fat reduction function protein extract. The auricularia polytricha blood fat reduction function protein extract preparation method comprises: 1) crushing auricularia polytricha bodies, carrying out extraction with water, and collecting a water-soluble substance to obtain a water-soluble auricularia polytricha extract; and 2) carrying out precipitation on the water-soluble auricularia polytricha extract with ammonium sulfate having a saturation degree of 30%, collecting a water-soluble substance, carrying out precipitation on the water-soluble substance with ammonium sulfate having a saturation degree of 80%, collecting the precipitate, and carrying out dialysis on the precipitate with deionized water to obtain the auricularia polytricha protein extract. With the auricularia polytricha blood fat reduction function protein extract, in various blood fat reduction indexes, total cholesterol content, triglyceride content and low density lipoprotein cholesterol content can be reduced, hyperlipemia conditions can be comprehensively released, concentrations of the three indexes in blood at an early blood fat reduction stage can be significantly reduced, and the auricularia polytricha blood fat reduction function protein extract can be applied in research and development fields of blood fat reduction health food and drugs.

Description

technical field [0001] The invention relates to a preparation method and an application of an auricularia auricula protein extract, in particular to a preparation method and an application of an auricularia auricula protein extract with blood lipid-lowering function. Background technique [0002] With the continuous improvement of people's living standards, a large intake of high-energy and high-fat foods, an increase in alcohol consumption, and a decrease in exercise have led to an obvious upward trend in the incidence of hyperlipidemia. Hyperlipidemia is the cause of coronary atherosclerosis. Heart disease (coronary heart disease), arteriosclerosis, fatty liver, diabetes, obesity and other important factors. Therefore, it is of great practical significance to find efficient and non-toxic blood lipid-lowering drugs. In recent years, in the treatment of hyperlipidemia, traditional Chinese medicine for lipid-lowering developed from natural medicinal and edible materials has ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/36C07K1/34C07K1/30A61K38/00A61K36/07A61P3/06A23L1/28A23L1/29A23L33/00
Inventor 赵爽刘宇许峰王守现王兰青耿小丽殷贝贝陈杰孟莉莉
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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