Lactobacillus probiotics CGMCC NO. 12422 and application of lactobacillus probiotics in preparing lipid-lowering drug

Abstract

The invention discloses a lactobacillus probiotic strain. The preservation number of the strain is CGMCC NO. 12422, and the preservation date is May, 6th, 2016. The 16S rRNA sequence of the strain is shown as SEQ ID NO: 2. The invention also discloses an application of the strain in preparing a lipid-lowering drug, food and a healthcare product. The strain is harmless to animals and has effects of regulating and lowering blood fat.

Description

technical field

[0001] The invention relates to a probiotic and its application, belonging to the field of microorganisms. Background technique

[0002] Human serum cholesterol content is closely related to cardiovascular diseases such as coronary heart disease, arteriosclerosis, and hyperlipidemia. The main symptoms of hyperlipidemia are excessive serum total cholesterol, low-density lipoprotein cholesterol, and triglycerides. How to control and lower the serum cholesterol level of the human body has become an important issue in promoting health. Many blood lipid-lowering drugs have a good lipid-lowering effect, but the side effects caused by long-term use have been confirmed by more and more studies. Looking for new ones with less side effects and more Research that can regulate the lowering of blood lipids has become a new demand.

[0003] Probiotics are defined as "a class of living microorganisms that, when ingested in appropriate quantities, can bring benefits to the ...

Examples

Embodiment 1

[0017] Embodiment 1. Isolation, screening and identification of lactic acid bacteria

[0018] 1. Isolation of Lactobacillus

[0019] 1) Take 100 μL of the sample from the sterile tube, add it to the EP tube pre-filled with 900 μL sterile PBS, and sequentially dilute the sample, and dilute the concentration of the marmot feces sample to 10 -6 times;

[0020] 2) Take 100 μL of samples with different dilutions and spread them on the MRS medium, and put them into the incubator;

[0021] 3) At 37°C, 0.5% CO 2 Cultivate in the environment for 48h;

[0022] 4) Take out the petri dish, use a sterile inoculation loop to pick colonies with different morphological characteristics, transfer them to a new MRS solid medium for purification, culture anaerobically at 37°C for 48 hours, transfer 3 times continuously, and transfer the purified strains at pH =3.5 of the liquid MRS culture, screening acid-resistant growth of excellent strains can be used for experiments or cryopreservation. ...

Embodiment 2

[0058] Embodiment two, lipid-lowering function evaluation of lactic acid bacteria

[0059] 1. In vitro evaluation of lipid-lowering function

[0060] (1) Configuration of cholesterol-containing medium

[0061] 1) MRS medium containing 0.1% bile salt: weigh bile salt powder at 0.1%, add it to 400ml prepared MRS liquid medium, mix thoroughly, and then sterilize by high pressure steam at 121°C for 15 minutes for use.

[0062] 2) MRS-CHOL (high cholesterol MRS medium): Accurately weigh 0.5g of cholesterol, dissolve it with a small amount of absolute ethanol and dilute to 50mL with a final concentration of 10.0mg / mL, filter through a microporous membrane with a pore size of 0.22μm After sterilization, add 5% into the sterilized MRS liquid medium (or MRS liquid medium containing 0.1% bile salt), so that the final concentration of cholesterol in the MRS liquid medium is 500 μg / mL.

[0063] (2) DAOS method to detect cholesterol content

[0064] 1) Principle

[0065] Hydrogen perox...

technical field

[0001] The invention relates to a probiotic and its application, belonging to the field of microorganisms. Background technique

[0002] Human serum cholesterol content is closely related to cardiovascular diseases such as coronary heart disease, arteriosclerosis, and hyperlipidemia. The main symptoms of hyperlipidemia are excessive serum total cholesterol, low-density lipoprotein cholesterol, and triglycerides. How to control and lower the serum cholesterol level of the human body has become an important issue in promoting health. Many blood lipid-lowering drugs have a good lipid-lowering effect, but the side effects caused by long-term use have been confirmed by more and more studies. Looking for new ones with less side effects and more Research that can regulate the lowering of blood lipids has become a new demand.

[0003] Probiotics are defined as "a class of living microorganisms that, when ingested in appropriate quantities, can bring benefits to the ...

Examples

Embodiment 1

[0017] Embodiment 1. Isolation, screening and identification of lactic acid bacteria

[0018] 1. Isolation of Lactobacillus

[0019] 1) Take 100 μL of the sample from the sterile tube, add it to the EP tube pre-filled with 900 μL sterile PBS, and sequentially dilute the sample, and dilute the concentration of the marmot feces sample to 10 -6 times;

[0020] 2) Take 100 μL of samples with different dilutions and spread them on the MRS medium, and put them into the incubator;

[0021] 3) At 37°C, 0.5% CO 2 Cultivate in the environment for 48h;

[0022] 4) Take out the petri dish, use a sterile inoculation loop to pick colonies with different morphological characteristics, transfer them to a new MRS solid medium for purification, culture anaerobically at 37°C for 48 hours, transfer 3 times continuously, and transfer the purified strains at pH =3.5 of the liquid MRS culture, screening acid-resistant growth of excellent strains can be used for experiments or cryopreservation. ...

Embodiment 2

[0058] Embodiment two, lipid-lowering function evaluation of lactic acid bacteria

[0059] 1. In vitro evaluation of lipid-lowering function

[0060] (1) Configuration of cholesterol-containing medium

[0061] 1) MRS medium containing 0.1% bile salt: weigh bile salt powder at 0.1%, add it to 400ml prepared MRS liquid medium, mix thoroughly, and then sterilize by high pressure steam at 121°C for 15 minutes for use.

[0062] 2) MRS-CHOL (high cholesterol MRS medium): Accurately weigh 0.5g of cholesterol, dissolve it with a small amount of absolute ethanol and dilute to 50mL with a final concentration of 10.0mg / mL, filter through a microporous membrane with a pore size of 0.22μm After sterilization, add 5% into the sterilized MRS liquid medium (or MRS liquid medium containing 0.1% bile salt), so that the final concentration of cholesterol in the MRS liquid medium is 500 μg / mL.

[0063] (2) DAOS method to detect cholesterol content

[0064] 1) Principle

[0065] Hydrogen perox...

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