Application of probiotics combined with dendrobium officinale, Phyllanthus emblica and extracts in improvement of diabetes and metabolic syndrome

A technology for metabolic syndrome and Dendrobium officinale, which is applied in the field of medicine and can solve problems such as diabetes and metabolic syndrome that have not been reported.

Pending Publication Date: 2019-12-24
NANJING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Dendrobium candidum, emblica, and probiotics have complementary medicinal effects, but so far, there is no report that the

Method used

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  • Application of probiotics combined with dendrobium officinale, Phyllanthus emblica and extracts in improvement of diabetes and metabolic syndrome
  • Application of probiotics combined with dendrobium officinale, Phyllanthus emblica and extracts in improvement of diabetes and metabolic syndrome
  • Application of probiotics combined with dendrobium officinale, Phyllanthus emblica and extracts in improvement of diabetes and metabolic syndrome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 In this experiment, Bifidobacterium and Streptococcus thermophilus were taken as examples. Dendrobium officinale, Emlical emblica and their extracts promote the growth of probiotics and inhibit the growth of harmful bacteria

[0025] Resuscitate Bifidobacterium, Streptococcus thermophilus, Escherichia coli and Staphylococcus aureus, use lactic acid bacteria medium for Bifidobacterium, M17 medium for Streptococcus thermophilus, LB medium for Escherichia coli, and beef extract peptone for Staphylococcus aureus culture medium. After overnight recovery at 37°C, add it to the culture medium at a ratio of 1:1000, the ratio of Dendrobium officinale and Emlical root is 1:1, mix well and give different concentrations (0, 0.02, 0.1, 0.2, 1, 2 mg / mL) of emblica extract, set 6 parallel wells for each concentration, after growing at 37°C for 8h, use OD 600 Detect the growth of Bifidobacterium, Streptococcus thermophilus, Escherichia coli and Staphylococcus aureus.

[00...

Embodiment 2

[0030] Example 2 Probiotics combined with Dendrobium candidum, Amla emblica and their extracts to control weight gain

[0031] Select 5-week-old male C57 mice (Qinglongshan Animal Experiment Center, Nanjing, Jiangsu), weighing 20-24 g, male, and divide them into control group (fed with high-fat feed), administration group 1 (Dendrobium officinale extract), Administration group 2 (Emblica emblica extract), administration group 3 (probiotics) and administration group 4 (combined action of Dendrobium candidum, Amla emblica and probiotics), 10 rats in each group, half male and half male. While feeding the high-fat diet, different drugs were orally administered, and the body weight of the mice in each group was recorded every 2 weeks for a total of 12 weeks, and the weight gain of the mice in the administration group was detected.

[0032] Table 2 Effects of probiotics combined with Dendrobium candidum and Emlical extracts on weight gain in mice

[0033]

[0034] *Compared with...

Embodiment 3

[0036] Example 3 Probiotics combined with Dendrobium candidum, Emlical seed and their extracts reduce blood sugar in diabetic mice induced by high-fat diet

[0037] Oral glucose tolerance test (OGTT) in high-fat diet-induced diabetic mice: 5-week-old C57 mice (Qinglongshan Animal Experiment Center, Nanjing, Jiangsu), weighing 20-24 g, male, were used to induce obesity in mice with high-fat diet . The modeled mice were randomly divided into 5 groups, respectively control group, Dendrobium candidum group (100mg / kg), Emlical seed group (100mg / kg), probiotic group (100mg / kg), Dendrobium candidum, Emlical seed extract Combined probiotics group (100mg / kg+100mg / kg+100mg / kg), 8 rats in each group, administered orally every day for 12 consecutive weeks. At the end of the experiment, the mice were fasted for 12 hours before the experiment, given glucose (2g / kg) by intragastric administration, and blood was collected from the tail to measure the blood sugar level at 0, 15, 30, 60, and 1...

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Abstract

The invention relates to the technical field of biology, in particular to probiotics, which is combined with dendrobium officinale, phyllanthus emblica and the extracts to regulate blood sugar and blood fat, improve diabetes mellitus and metabolic syndrome, slow down occurrence of related complications and further maintain metabolic stability of organisms. The probiotics is an active microorganismbeneficial to a host, can regulate intestinal flora balance, and can generate an exact health effect so as to improve the micro-ecological balance of the host and play a beneficial role; the phyllanthus emblica has the effects of regulating blood sugar, enhancing body immunity and the like. The dendrobium officinale and phyllanthus emblica are found to play a role in reducing blood sugar and blood fat; the dendrobium officinale and phyllanthus emblica components can provide a good environment for the growth of probiotics; meanwhile, the probiotics are promoted to regulate glucose uptake, andthe gastrointestinal tract stimulation effect generated by taking the probiotics alone is improved, so that the probiotics and the phyllanthus emblica can better play a role in reducing blood sugar, complications are reduced, and the material can be prepared into a pharmaceutical composition for preventing or treating diabetes and metabolic syndrome. Therefore, the invention provides a new application for improving diabetes mellitus and metabolic syndrome related diseases.

Description

technical field [0001] The invention belongs to the technical field of medicine, and specifically relates to a new application of probiotics combined with dendrobium officinale, emblica and extracts thereof to improve complications related to diabetes and metabolic syndrome. Background technique [0002] Diabetes is a disease of energy metabolism, which is mainly divided into type 1 diabetes (insulin-dependent diabetes) and type 2 diabetes (non-insulin-dependent diabetes). At present, there are about 366 million diabetic patients in the world, accounting for 6.4% of the world's population, and type 2 diabetic patients account for about 90-95% of the total number of diabetic patients. The pathogenesis of type 2 diabetes is very complex. In addition to changes in the human genome, eating habits or reduced physical activity leading to diabetes, changes in the structure of intestinal flora are related to the occurrence and development of diabetes. As an environmental factor, in...

Claims

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Application Information

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IPC IPC(8): A61K36/8984A61K45/06A61P3/10A61P3/00A61P3/06A61P3/04A61P19/06A61K35/745A61K35/747A61K35/744
CPCA61K35/744A61K35/745A61K35/747A61K36/47A61K36/8984A61K45/06A61P3/00A61P3/04A61P3/06A61P3/10A61P19/06A61K2300/00
Inventor 于倩倩徐郁蕊张瑜刘晓平宁兴海
Owner NANJING UNIV
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