Lactobacillus plantarum for improving lipid metabolism and muscle quality of meat poultry and application of lactobacillus plantarum

A technology of Lactobacillus plantarum and poultry fat, applied in the field of probiotics, can solve the problems of few researches on strains, achieve the effects of reducing drip loss, increasing meat production rate, and improving breeding benefits

Active Publication Date: 2021-04-30
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bile salts include sodium taurocholate, sodium glycocholate, sodium taurodeoxycholate, and sodium glycodeoxycholate, but there are few studies on strains that have different degradation abilities for the four substrates

Method used

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  • Lactobacillus plantarum for improving lipid metabolism and muscle quality of meat poultry and application of lactobacillus plantarum
  • Lactobacillus plantarum for improving lipid metabolism and muscle quality of meat poultry and application of lactobacillus plantarum
  • Lactobacillus plantarum for improving lipid metabolism and muscle quality of meat poultry and application of lactobacillus plantarum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Screening and preparation of Lactobacillus plantarum: Screening was started from 86 strains of lactic acid bacteria stored in the laboratory derived from fermented material and animal intestines.

[0028] Strain activation: Streak the strains stored at -80°C on the MRS solid medium for activation, and culture at 37°C for 24 hours; pick a single colony grown on the plate and inoculate it in 3mL MRS liquid medium, culture overnight at 37°C, 12000rpm Centrifuge for 1 min, discard the supernatant, and collect the cells; take 1 mL of 0.1M acetic acid-sodium acetate buffer (pH 5.0) to wash the cells once, remove the supernatant after centrifugation, and then take 1 mL of resuspended cells.

[0029] Preliminary screening of bile salt hydrolyzing enzyme activity by ninhydrin method: add 50 μL of bacterial suspension and 50 μL of 20 mM substrate (sodium taurocholate or sodium glycocholate) to a 1.5 mL eppendorf tube, mix, and bathe in water at 37 °C for 1 h; After the...

Embodiment 2

[0033] Embodiment 2: the application of Lactobacillus plantarum ZRR in the growth of poultry

[0034] Preparation of bacteria powder: inoculate frozen-preserved ZRR and CR into MRS liquid medium respectively, culture at 37°C for 24 hours, and subculture twice in this way to obtain the activated ZRR and CR strains. The activated strains were inoculated in MRS liquid medium and cultured at 37°C. The bacterial liquid was centrifuged, freeze-dried, pulverized, and mixed with auxiliary materials to make ZRR and CR freeze-dried powder, so that the number of live bacteria in the freeze-dried powder of lactic acid bacteria reached 1× 10 10 cfu / g.

[0035] Healthy 1-day-old Cherry Valley meat ducks were selected and randomly divided into 3 groups, with 6 replicates in each group and 24 ducks in each replicate (half male and half female), until the 42nd day for slaughter. Taking normal drinking water as the control, the test group added Lactobacillus plantarum CR and Lactobacillus pla...

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Abstract

The invention discloses lactobacillus plantarum for improving lipid metabolism and muscle quality of meat poultry and application of the lactobacillus plantarum. The lactobacillus plantarum is lactobacillus plantarum ZRR, the preservation number of the lactobacillus plantarum is CCTCC No: M2016281, and the lactobacillus plantarum can degrade different cholate at the same time and has the advantages of improving the growth performance and slaughter performance of the meat poultry, improving the lipid metabolism and muscle quality of the meat poultry and the like. The lactobacillus plantarum can be widely applied to meat poultry breeding, promotes healthy breeding of meat poultry, and improves breeding benefits.

Description

technical field [0001] The invention belongs to the technical field of probiotics, and in particular relates to a lactobacillus plantarum for improving lipid metabolism and muscle quality of poultry and an application thereof. Background technique [0002] With the expansion of the scale of meat and poultry farming and the improvement of production efficiency, its meat output has increased rapidly, and it has become the second largest meat product next to pork. The production performance is overemphasized in breeding, and high-fat diets are often used to feed meat and poultry. It leads to lipid metabolism disorder and ectopic fat deposition in the liver, which in turn leads to a decrease in feed conversion rate and muscle quality. Poultry lipids such as triglycerides, cholesterol and fatty acids are mainly synthesized in the liver and widely distributed in the abdomen, subcutaneous tissue and muscle tissue. They form a complex metabolic network with the processes of ingestio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/75A23K10/18C12N1/20C12R1/25
CPCA23K50/75A23K10/18C12N1/20A23V2400/169
Inventor 宦海琳林勇杨瑶闫俊书周维仁严敏
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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