The invention discloses bacillus amyloliquefaciens probiotics capable of producing phytase, and a preparation as well as a preparation method of the bacillus amyloliquefaciens probiotics. The gene GenBank accession number of the rRNA (Ribosomal Ribonucleic Acid) gene sequence of the bacillus amyloliquefaciens T6, 16S is JX081245. According to the method, a shallow tray fermentation culture method and a submerged fermentation culture method are applied to strain culture, and the specific steps include strain storage by glycerinum, strain activation, strain treatment in a shake flask, strain culture, load of the strain to a carrier, drying, inspection, and packaging to obtain a finished product. The novel probiotics preparation is prepared by using the bacillus amyloliquefaciens strain capable of producing the phytase, which is heat resistant, dryness resistant, acid resistant, alkali resistant and long in storage period, is stable in quality, and can be used for keeping intestinal microecological balance of livestock and poultry, ensuring that the growth and development of an animal in an ideal state reaches the maximum capacity of the animal, improving the feed digestion rate of the animal, improving the disease resistant capacity of the animal, promoting animal health, reducing the calcium phosphate addition amount in daily ration, reducing the excretion amount of the calcium phosphate in excrements of livestock and poultry, protecting environment and achieving the proposes of energy conservation and emission reduction.