The invention discloses a fat SVF
cell clinical high-efficiency preparation and
cryopreservation method, and applications thereof. The fat SVF
cell clinical high-efficiency preparation and
cryopreservation method comprises following steps: S1, fat samples are collected under aseptic conditions, and are preserved in a preserving fluid, and a washing liquid is added for pre-treatment of the fat samples; S2, after pre-treatment, the fat samples are subjected to centrifugalization so as to obtain
yellow fat tissue, the fat tissue is added into a preheated digestive juice, an obtained mixture is sealed, under constant temperature conditions, the fat tissue and the digestive juice are mixed to be uniform,
digestion is carried out until no large tissue block is left or even large tissue blocks are left, a large amount of fat is gathered, and then
centrifugation is carried out, wherein the volume ratio of the fat tissue to the preheated digestive juice is controlled to be 1:1; and S3, after
centrifugation, upper layer fat is removed, the washing liquid is added for resuspending and uniform mixing of an obtained residue, filtering is carried out, the washing liquid is added again,
centrifugation is carried out, and an obtained supernatant is removed so as to obtain fat SVF cells. According to the fat SVF
cell clinical high-efficiency preparation and
cryopreservation method, the washing liquid and the digestive juice are used for increase the
cell number, the cell living rate, and the purity of the obtained fat SVF cells, and the effect of the extracted SVF cells in
clinical treatment is ensured.