Pediococcus pentosaceus NHB-PpA9601 and application thereof

A technology of Pediococcus pentosaceus and bacterial agent, which is applied in the application, bacteria, antibacterial drugs and other directions, can solve the problems such as no report on the inhibitory effect of Streptococcus suis.

Active Publication Date: 2021-09-14
NEW HOPE LIUHE +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the inhibitory effect on Streptococcus suis, and most of the tests on antagonizing pathogenic ba...

Method used

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  • Pediococcus pentosaceus NHB-PpA9601 and application thereof
  • Pediococcus pentosaceus NHB-PpA9601 and application thereof
  • Pediococcus pentosaceus NHB-PpA9601 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0093] The preparation of embodiment 2 Pediococcus pentosaceae NHB-PpA9601 fermented liquid

[0094] Get Pediococcus pentosaceae NHB-PpA9601 (preservation number is CGMCC No.22646) seed solution (viable bacteria concentration is 10 9 CFU / mL) 1mL, inoculated in 100mL shake flask fermentation medium for shake flask fermentation culture; after shake flask fermentation, carry out 10L seed fermentation tank culture, take 70mL shake flask fermentation seed liquid and inoculate into 10L seed fermentation tank for seed culture , 10L fermentation tank liquid volume is 7L seed culture medium. After the cultivation, 3.5L of the seed liquid was inoculated into the fermentation medium in the 100L fermenter to carry out the fermentation culture, and the liquid volume of the 100L fermenter was 70L of the fermentation medium. After the fermentation, the number of viable bacteria in the fermentation broth was detected to be 6.2×10 9 CFU / mL, the fermentation broth was stored in the refrigerat...

Embodiment 3

[0103] Example 3 Probiotic verification of Pediococcus pentosaceae NHB-PpA9601

[0104] On the aseptic operating table, the concentration is 10 9 CFU / mL of pathogenic bacteria (enteropathogenic Escherichia coli, Staphylococcus aureus, Salmonella typhi, Salmonella choleraesuis, Salmonella gallinarum typhi, Salmonella pullorum, Salmonella typhimurium, Salmonella, Shigella, Capsula perfringens bacillus, Proteus penderii, Aeromonas hydrophila, Vibrio parahaemolyticus) bacteria suspension was added to nutrient agar cooled to 45 ℃ (for Clostridium perfringens, the culture medium was replaced with tryptone-sulfite- Cycloserine agar, culture conditions then need strict anaerobic conditions) medium (after sterilization) mix, prepare the pathogenic bacteria agar plate of about 4mm, the concentration of pathogenic bacteria in the agar plate is 10 9 CFU / mL. Place the sterilized Oxford cup on the culture medium, gently pressurize it so that it is in contact with the culture medium withou...

Embodiment 4

[0110] Example 4 Verification of stress resistance of Pediococcus pentosaceae NHB-PpA9601

[0111] 1. Determination of resistance to artificial gastric juice:

[0112] Take 10 mL of Pediococcus pentosaceae NHB-PpA9601 bacterial suspension (prepared according to the method described in Example 1) and place it in 90 mL (250 mL Erlenmeyer flask) of artificial gastric juice, shake at 37 ° C, 200 r / min at a constant temperature for 180 min; after the shaking is completed, Take 10mL of the sample solution to adjust the pH to 7.0, add 90mL of normal saline, shake at 37°C and 200r / min for 30min, and then perform colony counting on the dilution plate. The results are shown in Table 3. As can be seen from Table 3, the survival rate of Pediococcus pentosaceae NHB-PpA9601 in artificial gastric juice (containing enzyme) at pH 2.0 for 3 hours was 92.68%, indicating that the bacterial strain NHB-PpA9601 has higher acid resistance and can tolerate Stomach acid can smoothly reach the intesti...

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Abstract

The invention provides a pediococcus pentosaceus NHB-PpA9601. The pediococcus pentosaceus NHB-PpA9601 is separated from intestinal tracts of white feather broilers, and is preserved in China General Microbiological Culture Collection Center (CGMCC for short, the address is Institute of Microbiology, Chinese Academy of Sciences, 3 #, 1 # yard, West Beichen Road, Chaoyang District, Beijing, the postal code is 100101), the preservation number is CGMCC NO.22646, and the preservation date is May 31, 2021. The pediococcus pentosaceus NHB-PpA9601 has the advantages that the obvious probiotic property is realized, the growth and reproduction of pathogenic bacteria such as intestinal pathogenic escherichia coli, staphylococcus aureus, salmonella suis, salmonella choleraesuis, salmonella gallinarum, salmonella pullorum, salmonella typhimurium, salmonella, shigella, bacillus perfringens, proteus penneri, aeromonas hydrophila and vibrio parahaemolyticus can be effectively inhibited; the pediococcus pentosaceus NHB-PpA9601 can be used for producing a wet-based fermented feed, and can be used for effectively improving intestinal health and increasing economic benefits.

Description

technical field [0001] The invention belongs to the field of probiotics for feeding, in particular to a strain of Pediococcus pentosaceus (Pediococcuspentosaceus) NHB-PpA9601 and an application thereof. Background technique [0002] Lactic acid bacteria regulate intestinal pH through metabolites, thereby promoting animal growth, adjusting the structure and relative abundance of intestinal flora, thereby improving gastrointestinal function, improving growth performance and reducing morbidity. Pediococcus pentosaceae belongs to the genus Pediococcus of the Streptococcus family, can ferment glucose to produce lactic acid, and can inhibit pathogenic bacteria such as Aeromonas hydrophila, Listeria, and Bacillus cereus. However, there is no report on the inhibitory effect on Streptococcus suis, and most of the tests on antagonizing pathogenic bacteria are carried out in vitro, and whether it can exert an effective antibacterial effect in complex animals has not been reported yet. ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/18A23K50/75A61K35/744A61P1/12A61P31/04C12R1/01
CPCC12N1/20A23K10/18A23K50/75A61K35/744A61P1/12A61P31/04Y02A50/30
Inventor 张遨然尹望刘武蔡煕姮王红梅郭建强周航杨颜铱周桂莲李巍
Owner NEW HOPE LIUHE
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