Method for improving slaughter performance of Jinghai yellow chicken by MSTN genes

Abstract

The invention relates to the field of poultry breeding, in particular to a method for improving the slaughter performance of Jinghai yellow chicken by MSTN genes. The method includes the steps: extracting genome DNA (deoxyribonucleic acid) of a sample to be detected; amplifying the genome DNA by a specific primer with the sequence shown as SEQ ID NO: 1-2 to obtain 186bp target fragments; performing SSCP (single strand conformation polymorphism) analysis for the target fragments; developing colors by silver staining after gel electrophoresis to obtain DNA SSCP spectrum; screening individuals with gene type AA according to banding patterns of the atlas. Statistic analysis indicates that the slaughtering weight, the breast muscle weight, the leg muscle weight, the eviscerated weight and the semi-eviscerated weight of the type AA individuals are remarkably higher than those of type GG individuals (P<0.05). The homozygotic type AA individuals can be fixed in offspring, and the detection method is simple, rapid and not affected by external environments.

Description

technical field [0001] The invention relates to the field of poultry breeding, in particular to a method for improving the slaughter performance of Jinghai yellow chicken by using MSTN gene. Background technique [0002] With the development of the national economy and the continuous improvement of people's living standards, people's demand for high-quality broilers is becoming more and more urgent, but high-quality broilers generally have problems such as slow production speed and poor meat production capacity. The improvement of the slaughter performance of high-quality broilers has always been concerned by breeders, while the use of traditional breeding methods has made slow progress, forcing and stimulating breeders to find new ways and methods. Finding the molecular genetic markers of slaughter performance for marker-assisted selection will accelerate the improvement of slaughter performance of high-quality broilers. [0003] The MSTN gene, also known as the myostatin ...

AI Technical Summary

Problems solved by technology

[0002] With the development of the national economy and the continuous improvement of people's living standards, people's demand for high-quality broilers is becoming more and more urgent, but high-quality broilers generally have problems such as slow production speed and poor meat production capacity.

The improvement of the slaughter performance of high-quality broilers has always been concerned by breeders, while the use of traditional breeding methods has made slow progress, forcing and stimulating breeders to find new ways and methods

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