44 results about "Breast muscle" patented technology

The invention relates to a preparation method and application of a gamma-PGA-rich feed additive for broiler chickens, and the technical scheme for solving the problems is as follows: screening bacillus licheniformis DY-0089 for high yield of gamma-PGA from fermented foods such as fermented soya beans, fermented bean curds and natto, inoculating the bacillus licheniformis DY-0089 on a solid fermentation culture medium, fermenting for 24-96 hours at 28-45 DEG C, drying in a drying oven at 55-70 DEG C, and crushing to obtain the gamma-PGA-rich feed additive for broiler chickens. And preparing the feed additive rich in gamma-PGA. Research finds that when 1% of the gamma-PGA-rich feed additive is fed to broilers, the immune function of the body can be enhanced, cecum microbiota can be improved, cecum metabonomics can be adjusted, then the growth performance and feed conversion rate of the broilers are improved, serum biochemical indexes, oxidation resistance, slaughter performance, pectoral muscle nutritional ingredients and the like of the broilers are basically not affected, and the feed additive is suitable for large-scale production. And a theoretical basis is provided for development and application of the gamma-PGA-rich feed additive in animal breeding, especially broiler breeding.

The application discloses a SNP marker combination for broiler breast intramuscular fat content prediction and assisted breeding and application thereof, and belongs to the technical field of assisted breeding.The application aims to provide a method for broiler breast intramuscular fat content prediction and assisted breeding.The application provides 331 SNP markers related to broiler breast intramuscular fat content, which are used for identifying broiler breast intramuscular fat content.

The invention relates to the technical field of poultry quality regulation and control. According to the meat duck specific pectoral muscle metabolism marker and the screening method thereof, flavor substances of yellow feather broilers and three varieties of meat ducks are extracted and analyzed, accurate identification of the meat duck fishy smell marker is achieved through multivariate statistical analysis, and the screening method has the advantages that the screening efficiency is high, and the screening cost is low. And the markers comprise heptanal, nonanal, E-2-heptenal, E-2-octenal and 1-octylene-3-alcohol. The invention further discloses a method for preparing the biomarker. The determination of the meat duck breast muscle fishy smell metabolism marker provides technical support for reducing and removing fishy smell of duck meat and improving the quality, so that the economic benefits of the meat ducks are improved.

The present application belongs to the field of molecular biology technology and breeding of Muxuan black-bone chickens, and particularly relates to a PMEL17 gene for breast muscle blackness of Muxuan black-bone chickens and a genetic marker method thereof. The nucleotide sequence of the gene is shown as SEQ ID NO. 1. The marker method is to design a primer pair, take the genomic DNA of Muxuan black-bone chickens as a template, perform PCR amplification, obtain a sequence shown as SEQ ID NO. 4, identify whether the sequence has a mutation, and analyze the correlation between the mutation and the breast muscle blackness of Muxuan black-bone chickens. The screened gene is a gene related to the breast muscle blackness of Muxuan black-bone chickens, and further screening of a SNP related to the trait at a 553th site of the PMEL17 gene sequence can be used as a genetic marker for auxiliary selection of Muxuan black-bone chickens. Through the genetic marker, Muxuan black-bone chickens with more melanin deposition and darker color can be selected and bred, which has important significance and application value for the genetic breeding of Muxuan black-bone chickens.

The invention discloses a meat duck pectoralis determination system and method. The system comprises a multi-modal pectoralis image fusion divider module, a muscle three-dimensional feature principal component fusion model module, a heterogeneous pectoralis multi-index grading model module, a poultry meat muscle parameter management analysis platform, a meat duck pectoralis parameter acquisition module and a parameter result output module. The method comprises the following steps: acquiring parameters such as muscle fiber diameter and intermuscular fat content through a meat duck pectoralis parameter acquisition module, storing and associating the parameters through a poultry meat muscle parameter management and analysis platform, and respectively transmitting the parameters to a multi-modal pectoralis image fusion divider and a muscle three-dimensional feature principal component fusion model; the former processes a multi-source image to generate a segmented image, the latter processes parameters to generate a feature vector, the two data are transmitted into a heterogeneous pectoralis multi-index grading model to analyze and judge grades, and finally, a parameter result output module integrates an output result. The meat duck breast muscle measuring efficiency and accuracy are improved, and the quality control requirement of the meat duck industry is met.

The application provides a SNP molecular marker and a breeding application method which are significantly related to the content of chicken meat flavor substance (E, E)-2,4-decadienal. The SNP molecular marker is rs316165567, contains a nucleotide sequence with a polymorphism of C / A at 2,519,798 bp of chicken chromosome 14, and when the genotype of the site is AA, the content of (E, E)-2,4-decadienal of the chicken meat sample is higher. The established molecular assisted breeding technology can early select the high-quality chicken flavor quality traits by determining the genotype of the SNP site of the measured chicken, can improve the content of (E, E)-2,4-decadienal of the chicken breast, and has great industrial application value and economic and scientific research value.

The invention relates to the technical field of livestock and poultry breeding, and particularly discloses combined application of prebiotics and probiotics, the prebiotics are propyl inulin nano prebiotics, and the probiotics are plant lactobacillus. 0.1 g / kg of propyl inulin nano prebiotics and 109 CFU / kg of plant lactobacillus are added into the broiler feed, so that the weight gain ratio of the broiler feed is reduced to 1.67, the pectoral muscle rate is increased to 30.4%, the anti-oxidation defense and immune regulation capabilities are improved, the intestinal microbial metabolic pathway is remarkably changed, and the growth of the broiler feed is promoted. The combined application of prebiotics and probiotics realizes triple improvement of broiler chicken production performance, immunocompetence and intestinal health, and provides a new way for promoting green and safe development of broiler chicken breeding industry.

The application provides a kind of meat sample drip loss continuous determination device and method, it is related to meat sample determination technical field, including refrigerated cabinet is equipped with multiple refrigeration cavities, refrigeration cavity is sealingly connected with closed door, the rear end of closed door is connected with bearing frame, bearing frame is slidingly connected in refrigeration cavity;Two weight monitoring mechanisms are arranged in the upper portion of bearing frame, the front end of closed door is connected with shell, digital display controller is arranged in shell;Weight monitoring mechanism is connected with meat sample positioning mechanism;Suction mechanism is arranged below bearing frame.The water film formed in suction mechanism is contacted with the surface of breast muscle sample or leg muscle sample, to avoid friction and extrusion on breast muscle sample or leg muscle sample, digital display controller cooperates with weight monitoring mechanism and suction mechanism, continuously and synchronously obtains the drip loss data of breast muscle sample, leg muscle sample multiple time points, realizes in the same closed space, continuously determines breast muscle sample and leg muscle sample.

The invention belongs to the technical field of animal feeding, and discloses a feeding method and device for animal sustainable research, the poultry growth cycle is divided, the whole growth cycle is divided into a brooding period, a growing period and an egg laying period / maintenance period, and staged running training is performed according to the divided growth cycle; health monitoring and adjustment are carried out, the speed is reduced when the feed intake per day is reduced by more than 15%, and electrolyte is supplemented when the water content of poultry manure is greater than 75%; according to the method, anatomical sampling detection is performed monthly, and leg muscle / pectoral muscle ratio, tibia bone density and athletic ability tests are performed, so that poultry can be driven to move when the poultry is fed, and regular movement can promote cardiopulmonary function development, improve metabolism efficiency, enhance disease resistance and reduce the incidence rate of respiratory tract and digestive tract diseases; exercise consumes excess energy, excessive accumulation of visceral fat is avoided, and the probability of metabolic diseases such as fatty liver is reduced.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker remarkably related to the content of a chicken flavor substance (E, E)-2, 4-decadienal and a breeding application method. The SNP molecular marker is rs316165567 and contains nucleotide sequences with C / A polymorphism at 2nd, 519th and 798bp positions on a chicken chromosome 14, and when the genotype of the site is AA, the content of (E, E)-2, 4-decadienal of a corresponding chicken sample is high. According to the established molecular assisted breeding technology, the flavor quality character of the high-quality broiler chicken is selected in an early stage by determining the genotype of the SNP site of the chicken to be detected, the content of the chicken breast muscle (E, E)-2, 4-decadienal can be increased, and the molecular assisted breeding technology has great industrial application value and economic and scientific research value.

The invention discloses a lemongrass addition feeding method suitable for meat duck on-net house breeding, which comprises the following steps: pretreatment of raw materials, preparation and use of a mixed solution, production of lemongrass fermented grass powder, feeding of the lemongrass fermented grass powder and evaluation of meat duck production performance. The method specifically comprises the following steps: lemongrass mowing operation, rubbing and squeezing operation, preparation of a duck shed spraying mixed solution, preparation of a meat duck drinking water mixed solution, a first fermentation stage, a second fermentation stage, evaluation aiming at the growth speed, detection aiming at the breast muscle oleic acid content and detection aiming at the leg muscle oleic acid content. Through the improvement of the technical scheme, the environment of a duck shed can be improved, the quality of meat ducks can be improved, the breeding cost can be reduced, the production performance of the Muscovy ducks can be remarkably improved by adding medium and high doses of citronella fermented grass meal into the feed, particularly, the feed-gain ratio is remarkably reduced, the content of breast muscle oleic acid is increased, and the economic benefit is increased. The feed additive has remarkable economic and social benefits, and meets the development requirements of modern breeding industry.

The application discloses a SNP molecular marker related to chicken carcass weight traits and application thereof, the molecular marker site is located in an intron region of RNASEH2B gene, GRCg6a version genome chr1:171223058, 1174 chicken crossbreeding population individuals are sequenced by using resequencing technology, and whole genome association analysis is carried out, a SNP site significantly related to a carcass phenotype is found, the SNP marker has three genotypes, AA, CC and AC, wherein CC corresponds to higher carcass weight, breast muscle weight and leg muscle weight, SNP frequency analysis of the SNP site in low-weight chicken breeds and high-weight chicken breeds is carried out, and it is found that the SNP frequency distribution has a significant difference in the low-weight chicken breeds and the high-weight chicken breeds, in the high-weight chicken, C is the dominant allele, and in the low-weight chicken, A is the dominant allele, and early selection of chicken carcass traits can accelerate chicken genetic breeding by selecting the C / C genotype of the SNP site.

The present application relates to the technical field of biological breeding and artificial intelligence, and particularly relates to a poultry breast muscle weight prediction method, device and electronic equipment. The method comprises the following steps: using an image enhancement network to perform enhancement processing on a digital X-ray radiography image of poultry, to obtain an enhanced digital X-ray radiography image; using an image segmentation network to perform breast muscle region segmentation on the enhanced digital X-ray radiography image, to obtain breast muscle region information; based on the breast muscle region information, extracting geometric features of the breast muscle region; inputting the geometric features into a trained machine learning model, and outputting a prediction result of the breast muscle weight of the poultry. The present application solves the defects of low efficiency and inability to realize automatic batch measurement caused by manually segmenting and weighing to obtain the breast muscle weight of poultry in the prior art, and realizes rapid and automatic prediction of the breast muscle weight of poultry.

The invention relates to the technical field of biology, in particular to an SV molecular marker related to chicken breast muscle weight character and application of the SV molecular marker. The SV molecular marker of the AK5 gene related to the pectoral muscle weight character is found, and the SV molecular marker is a section of 339 bp deletion variation located in a region 18831318-18831656 on a No.8 chromosome of a chicken T2T genome (GCA024206055.2); in addition, a corresponding primer pair and a detection product are designed based on the SV molecular marker, so that prediction and screening of chicken breast muscle weight traits are realized. Results of the specific embodiment of the invention show that the chicken breast muscle weight can be accurately determined by using the SV molecular marker, the primer pair and the detection product provided by the invention. Therefore, the invention provides a new molecular marker for assisted selective breeding.

The invention relates to application of a chicken miRNA-3528 gene SNP molecular marker in chicken economic character improvement breeding and chicken blood biochemical index evaluation, and belongs to the technical field of biological breeding. The nucleotide sequence of the SNP molecular marker is as shown in SEQ ID NO.1, and the 12th basic group from the 5'end is A or G; the economic traits are carcass traits and / or growth traits and / or meat quality traits; the carcass traits comprise full eviscerating weight; the growth traits comprise birth weight, 2-week weight, 4-week weight and 6-week weight; the meat quality characters comprise sebum rate and pectoral muscle density. By screening and detecting the genotype of the SNP molecular marker of the chicken miRNA-3528 gene on the DNA level and selecting GG genotype cocks and hens for breeding, the accuracy of chicken body size character selection can be improved, the breeding process of new broiler varieties is accelerated, the production performance of chickens is effectively improved, and remarkable economic benefits are obtained.

The application relates to a production method and application of combined fermentation of bupleurum and lonicera japonica residues by bacillus velezensis and pediococcus acidilactici, and belongs to the technical field of biotechnology. The bupleurum and lonicera japonica residues are treated by adopting a process of enzymolysis first and then fermentation, wherein the composite enzyme comprises cellulase, beta-glucanase, xylanase, beta-mannanase and phytase; and the fermentation bacteria comprise bacillus velezensis and pediococcus acidilactici. The fermented bupleurum and lonicera japonica residues provided by the application have a high release amount of active ingredients (chlorogenic acid, forsythoside and baicalin), and the addition ratio in broiler chickens is 2.0%-4.0%. The application effect includes improving growth performance, improving the levels of anti-inflammatory factor IL-10 and serum immunoglobulin IgM of the body, improving the muscle quality and flavor characteristics of the breast muscle, and improving the contents of inosinic acid, intramuscular protein, flavor amino acid and total amino acid. The product can not only improve the yield and quality of livestock products, but also relieve the pressure on the environment caused by solid waste residues, and has a good market prospect.

The invention relates to the cross technical field of artificial intelligence and intelligent agriculture, discloses a poultry motion distribution analysis and feed feeding method and system integrated with intelligent sensing, and aims to solve the problems of high decision delay, high misjudgment rate and resource waste caused by dependence on indirect behavior inference in the prior art. According to the method, poultry breast muscle temperature, micro-macro motion and identity data are collected in real time through a multi-mode sensing terminal, a group physiological state space-time matrix is constructed at an edge gateway, and starvation state distribution is predicted based on a hidden Markov model; and the distributed feeder autonomously executes accurate feeding according to the locally received probability graph and the dynamic threshold value. The system comprises a sensing terminal, an edge computing gateway and an intelligent feeding actuator, and a closed-loop architecture with in-situ sensing, edge analysis and autonomous response is formed. According to the application, low-delay and high-precision response to the real hunger demand of the poultry is realized, the feeding efficiency is remarkably improved, and the energy consumption and the communication overhead are reduced.

The technology as disclosed herein includes systems, methods, and compositions for detection of disease in a subject using spectroscopy and includes more effective detection of muscular myopathies in commercially farmed animals using infrared spectroscopy, and includes detection of Wooden Breast, White Striping, and / or Spaghetti Meat in broiler chickens utilizing near infrared spectroscopy. Implementations of the systems, methods, and compositions provided herein include and / or utilize and / or provide for detection of Wooden Breast, White Striping, and / or Spaghetti Meat in broiler chickens utilizing near infrared spectroscopy of breast area of live birds for prediction of taurine levels in the muscle.

The technology as disclosed herein includes systems, methods, and compositions for detection of disease in a subject using spectroscopy and includes more effective detection of muscular myopathies in commercially farmed animals using infrared spectroscopy, and includes detection of Wooden Breast, White Striping, and / or Spaghetti Meat in broiler chickens utilizing near infrared spectroscopy. Implementations of the systems, methods, and compositions provided herein include and / or utilize and / or provide for detection of Wooden Breast, White Striping, and / or Spaghetti Meat in broiler chickens utilizing near infrared spectroscopy of breast area of live birds for prediction of taurine levels in the muscle.

The invention discloses application of long-chain non-coding LncRNA LMRF in improvement of chicken weight and slaughter performance, and belongs to the technical field of gene engineering. Through specific overexpression of LncRNA LMRF in chicken liver tissues, compared with a control group, the average weight of chicken in an experimental group during slaughtering is increased by 185.00 g, the total eviscerating weight is increased by 179.38 g, the pectoral muscle rate is increased by 1.31 times, and the leg muscle rate is increased by 1.20 times. The long-chain non-coding LncRNA LMRF promotes growth and development of chicken muscles by increasing the muscle fiber diameter and muscle fiber area, so that the weight and slaughter performance of the chicken are improved.

This invention discloses a method for breeding a breeding stock, comprising the following steps: Step 1: Determining the combining ability testing scheme for the parent generation of Kebai chickens; Step 2: Analyzing the combining ability testing data of the parent generation and screening out commercial generations; Step 3: Measuring the combining ability of the commercial generations; Step 4: Analyzing the data of the commercial generations; Step 5: Measuring the meat quality of the commercial broilers; Step 6: Analyzing the production performance of the commercial generations and their parent generations according to Step 3; By measuring the combining ability of the parent generation of Kebai chickens, corresponding commercial generations are screened out, and the performance of the commercial generations is measured. The analysis results show that the egg production of 66-week-old breeding chickens is 5-8 fewer eggs than that of high-yielding commercial laying hens, while the various performance characteristics of the commercial generations are more than 10% higher than those of "817". The breast and leg muscles are basically similar to those of "817", and the meat quality also has a significant advantage. The breeding benefits are significant, and it has good market promotion prospects.

The present invention discloses a molecular marker related to the content of carnosine in duck meat and its application. The present invention relates to a molecular marker-assisted selection technology in the field of animal genetic breeding technology, and in particular to a molecular marker related to the content of carnosine in duck meat and its application. By detecting the polymorphism or genotype of two SNPs, SNP1 and SNP2, it is found that SNP1 is a site on duck chromosome 2, its nucleotide type is T or C, and it is the 511th nucleotide of sequence 1 in the sequence list, and the SNP2 site is a site on duck chromosome 7, its nucleotide type is T or C, and it is the 74th nucleotide of sequence 4 in the sequence list. When breeding duck breeds with high carnosine content in breast muscle, it is best to select ducks with SNP1 genotype of TT and SNP2 genotype of TT as parents for breeding. The present invention can be used to assist in identifying the content of carnosine in duck meat and perform early screening and auxiliary breeding of duck breeds.

The invention belongs to the technical field of poultry breeding, and discloses siRNA for targeted inhibition of chicken IMPAD1 expression and application thereof. The siRNA comprises IMPAD1-591 (IMPAD1-591 The siRNA can inhibit the expression of the chicken IMPAD1 gene in a targeted manner, after the siRNA is transferred into chicken primary myoblasts, the expression of the chicken IMPAD1 gene can be effectively reduced, the inhibition efficiency reaches 50% or above, and the knock-down efficiency is very high; the siRNA identifies the gene function by specifically knocking down the expression of the chicken IMPAD1 gene in vitro, can be used as a molecular marker to be applied to genetic breeding of breast muscle development of high-quality chicken, and has great economic value and scientific research value.

The invention discloses application of a MUSTN1 gene in regulation of chicken carcass traits, and belongs to the technical field of gene engineering. According to the invention, the MUSTN1 gene is specifically overexpressed in chicken muscle tissues, so that key slaughter traits such as pectoral muscle rate and leg muscle rate can be remarkably improved. The data of the embodiment shows that the pectoral muscle rate of a test group is obviously improved (Plt: 0.05) compared with that of a control group, the leg muscle rate also presents a synchronous improvement trend, and the edible meat product proportion is directly increased. The overexpression of the MUSTN1 gene not only improves the slaughter traits, but also promotes the overall growth of chickens. A test result shows that under the same feeding condition, the average weight of a test group with overexpressed MUSTN1 is obviously higher than that of a control group (Plt; 0.05), and the gene has the effect of increasing the growth speed.

The application discloses a molecular marker related to a duck breast muscle aldehyde phospholipid content character and application thereof. The application relates to the technical field of animal genetics and breeding, and particularly relates to a molecular marker related to a duck breast muscle aldehyde phospholipid content character and application thereof. The application comprises detecting the genotype of a SNP in a duck genome, and identifying or assisting in identifying the duck breast muscle aldehyde phospholipid content according to the genotype. The SNP is a site on a duck chromosome 21, and the nucleotide type is G or A, which is the 80th nucleotide in sequence 1 in the sequence table. The application can be used for predicting the duck breast muscle aldehyde phospholipid content and duck breeding. The substance for detecting the SNP polymorphism and genotype can be combined with other substances (such as a substance for detecting a single nucleotide polymorphism or a genotype of a molecular marker related to the duck breast muscle aldehyde phospholipid content) to prepare a product for identifying a duck with high breast muscle aldehyde phospholipid content.

The present invention relates to an additive to poultry feed including nitrates, nitrites or their mixtures that reduces the incidence of pectoral myopathy in poultry, for example, wooden breast myopathy in broiler chickens. In one aspect, a method for reducing the incidence of pectoral myopathy in poultry is disclosed. The method includes feeding poultry a feed having one or more nitrates, one or more nitrites, or a mixture thereof, wherein the amount of nitrates and / or nitrites in the feed is 3 g to 2,500 g per ton of feed.

The application discloses a circular RNA circCLASP2 of chicken, a nucleotide sequence of which is shown as SEQ ID NO: 1, and provides detection primers thereof, nucleotide sequences of which are shown as SEQ ID NO: 2-3. The application verifies the existence of a circular transcript circCLASP2 of a CLASP2 gene, proves that the circular transcript is resistant to RNase, and clones the full length of the circular transcript. The application determines through experiments that the circCLASP2 is highly expressed in breast muscle and leg muscle of white-feathered broilers, and is lowly expressed in Dawei mountain chickens; the circCLASP2 is time-sequentially expressed in white-feathered broiler muscle in the embryonic period, and has no obvious change in Dawei mountain chickens. The application verifies that the circCLASP2 mainly exists in cytoplasm through nucleocytoplasmic quantification. The application constructs an overexpression vector of the circCLASP2 and transfects myoblasts, and proves that overexpression of the circCLASP2 can improve the expression of proliferation marker genes and differentiation marker genes, and promote proliferation and differentiation of the myoblasts.

The invention provides application of a galega orientalis extract in preparation of a feed additive for improving poultry meat quality, and belongs to the field of feed additives. The capsella orientalis extract is obtained by extracting with a 50% ethanol aqueous solution and purifying with macroporous resin. The feed is used for improving the breast muscle quality, meat color, meat quality and physical and chemical quality of meat chickens. Moreover, the galega orientalis extract can promote the growth of beneficial bacteria by adjusting poultry intestinal microbiota, so that the galega orientalis extract is remarkably associated with poultry meat quality indexes.

The invention discloses application of MUSTN1 in promoting weight gain and intramuscular fat deposition of chickens, and belongs to the technical field of biological agriculture. According to the invention, the MUSTN1 gene and the encoding protein thereof are creatively applied, so that the synergistic improvement of chicken weight gain and intramuscular fat deposition is realized. The invention reveals for the first time that the over-expressed MUSTN1 gene can significantly increase the intramuscular fat (IMF) content while promoting the chicken weight increase, and effectively solves the long-standing technical bottleneck that the growth speed is negatively related to the meat flavor in commercial broiler chicken breeding. Test data show that the MUSTN1 overexpression enables the breast muscle IMF content of the Gushi chicken to be increased from 1.47% to 2.60%, the leg muscle IMF content of the Gushi chicken to be increased from 3.49% to 4.13%, and the weight of the Gushi chicken to be increased from 1507 g to 1707 g.