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Method for evaluating effective substance group of yeast culture by using metabonomics technology

A technology of yeast culture and metabolomics, which is applied in the field of microbial effective group evaluation, and can solve problems such as unevaluated yeast culture effective group

Active Publication Date: 2019-06-07
CHANGCHUN BORUI FEED
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there has been no report on the method of evaluating the effective group of yeast culture by using metabolomics technology.

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  • Method for evaluating effective substance group of yeast culture by using metabonomics technology
  • Method for evaluating effective substance group of yeast culture by using metabonomics technology
  • Method for evaluating effective substance group of yeast culture by using metabonomics technology

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Embodiment 1

[0074] Prepared raw materials: AA broiler chickens were purchased from Jilin Dexiang Company; Saccharomyces cerevisiae culture was provided by Jilin Agricultural University Jinong Borui R&D Center; Danongwei Yikang VXP was purchased from Danongwei China Co., Ltd.;

[0075] N, O-bis(trimethylsilane) trifluoroacetamide (BSTFA containing 1% TMCS), and trimethylchlorosilane are TMCS of Sigma-Aldrich Company of the United States; chromatographically pure pyridine of Shanghai Aladdin Biochemical Technology Co., Ltd.;

[0076] N-hexane from TEDIA Company of the United States, methoxyamine hydrochloride from Sigma-Aldrich Company of the United States.

[0077] The Agilent7890A / 5975C gas chromatography-mass spectrometer of Agilent Corporation of the United States is equipped with an Agilent chemical workstation: MSD Chem station (E. mm×0.25μm) quartz capillary column. MIKRO-22R desktop low-temperature refrigerated centrifuge from Hettich, Germany, and FD-1 freeze dryer from Beijing Bo...

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Abstract

The invention relates to the field of microbial effective substance group evaluation, in particular to a method for evaluating the effective substance group of yeast culture by using the metabonomicstechnology. The effective substance group of yeast culture is analyzed by using the detection technology in metabonomics, and the mathematical thinking of multivariable dimensionality reduction is applied to the digging of the composition of the effective substance group. According to the composition of the effective substance group, the composition relations of the effective substance group are simulated by using chemical substances, and combination is carried out. Then, the efficacy of the effective substance group is verified by animal feeding test. It is concluded that the effective substance group of yeast culture as a dietary additive can significantly improve the growth performance, immune function, antioxidant capacity and slaughter performance of broilers.

Description

technical field [0001] The invention relates to the field of microbial effective group evaluation, in particular to a method for evaluating the effective group of yeast culture by using metabolomics technology. Background technique [0002] Metabolomics is an emerging science of systems biology, an important part of systems biology, and a science developed after genomics, proteome and transcriptomics that constitute systems biology. The concept of metabolomics was first proposed by the metabolic profiling analysis proposed by Devaux et al. in 1971. They used gas chromatography-mass spectrometry (GC-MS) to qualitatively and quantitatively determine the metabolites in the body fluids of the subjects. This method is mainly used to screen and diagnose certain diseases and is still in use today. [0003] Metabolomics is the use of modern biological analysis methods to study small molecular metabolites (molecular mass <1000) related to metabolic pathways in organisms or cells ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/50G01N30/02G01N30/06C12Q1/689C12Q1/04
CPCY02A90/10
Inventor 甄玉国孙喆王涛张学峰赵巍陈雪赵小丽郑艳秋张玲玲张维刚谭胜男
Owner CHANGCHUN BORUI FEED
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