270 results about "Chenodeoxycholic acid" patented technology

The invention provides a preparation method of ursodeoxycholic acid. Commercial available chenodeoxycholic acid is taken as raw materials, the ursodeoxycholic acid is obtained by four steps including selective oxidation, esterification, deoxidation and hydrolyzation, and the total yield is 85.7%. In a mixture of acetone and water, NBS is used for selective oxidation of hydroxy at C- 7 bit of the chenodeoxycholic acid, and the selective oxidation possesses excellent selectivity and high yield. NaBH14/CeC13 may be used to deoxidize carbonyl at C-7 bit into hydroxy, and the ratio of alpha/beta is as high as 5/95.

The invention relates to an industrialized preparing method of extracting and purifying chenodeoxycholic acid from fowl and animal biles. Its process includes the steps of saponifying bile by lye, preparing calcium salt deposit of total bile acid, decolorizing by oxydol and active carbon, converting calcium salt deposit of chenodeoxycholic acid by sodium carbonate and HCl, purifying by the marcoporous absorbing resin, and so on. It has advantages of friend environment, safety and innocuity, low cost and be easy for large-scale operation.

A chemical-enzyme method of preparing ursodeoxycholic acid is disclosed. The method adopts chenodeoxycholic acid as an initial substrate, and prepares the ursodeoxycholic acid through a chemical process and a bio-enzyme process in order, wherein 7-KLCA reductase is adopted as a biological catalyst. A situation that an oxidant residual in a process of preparing 7-ketolithocholic acid through a chemical manner causes later enzyme inactivation in the prior art does not occur. A product prepared by the method is high in ee value and low in comprehensive cost.

The invention discloses a preparation method of ursodesoxycholic acid, which comprises the flowing steps of: a. carrying out 3-bit esterification on chenodeoxycholic acid to obtain 3-esterification protected chenodeoxycholic acid; b. carrying out 7-bit oxidation reaction on 3-bit ester to obtain 3-estergroup-7-oxocompounds; c. carrying out hydrolysis reaction or reduction reaction on the 3-estergroup-7-oxocompounds; d, carrying out 7-bit reduction reaction on 3a-hydroxy-7-oxo-5 beta-cholic acid or hydrolysis reaction on 3a-estergroup-7 beta- hydroxyl-5 beta-cholic acid to obtain crude products of the ursodesoxycholic acid; e. forming salt through the crude products of the ursodesoxycholic acid and organic base; and f. carrying out steps such as water adding dissolution, acid adding crystallization and the like on ursodesoxycholate to obtain pure ursodesoxycholic acid products with the purity higher than 99.0 percent. The invention aims at overcoming the defects in the prior art to provide a novel method for preparing the ursodesoxycholic acid, and the ursodesoxycholic acid prepared by the method has high yield and high purity.

The feature of the invention includes: adopting frozen chicken gallbladder slicing, heating to 70 degree centigrade, adding 10% weight of the bile boiling for 24 hours, reflux cooling, adjusting the pH value by hydrochloric acid to gain cream, washing to neutrality; adding 95% alcohol and 10% diatomite, reflux cooling and adding active carbon, reflux, cooling and filtering, adding petrol to the filtrate to take degreasing, reflux, standing, separating alcohol liquid and washing to neutrality in reaction kettle, adding 95% alcohol, after resolving, adding barium chloride and active carbon, reflux, hot filtering, concentrating white crystal from the filtrate, washing, and adding water and sodium carbonate water solution, heating and reflux, filtering, adjusting the pH value, drying. The invention has simple technology, low cost, and could drastically separate cholesterol, lithocholic acid, and cholic acid from gall, and improves the purity.

Use of chenodeoxycholic acid is disclosed to reduce adipose tissue and thereby reduce weight in mammals. The chenodeoxycholic acid can be administered orally through the use of a tablet, pill, capsule or liquid suspension.

A process for separating the chenodeoxycholic acid from pig's gall and purifying it includes such steps as preparing general cholic acid from the mother liquid generated by extracting the cholerythrin from pig's gall, saponifying, regulating pH value to obtain crude chenodeoxycholic acid, decoloring, defatting, preparing the deposit of barium chenodeoxycholate, reacting on potassium carbonate to remove Ba, regulating pH value, and purifying by silicon gel column.

The invention discloses a method for preparing chenodeoxycholic acid in pig bile by an esterification method, which comprises the following steps that: methanol, concentrated sulfuric acid and sodium hydroxide are used for esterification; acetic anhydride is used for acidylation; sherwood oil is used for extraction; the primary crystallization and the secondary crystallization are realized through ethanol and the sherwood oil; the sodium hydroxide is used for hydrolytic acidification; ethyl acetate and active carbon are used for crystallization; and the chenodeoxycholic acid is obtained through drying. The chenodeoxycholic acid is prepared by the method disclosed by the invention, the preparation method is simple, safety and environment protection are realized, the method can be used for large-scale industrial production, the content of the extracted chenodeoxycholic acid is higher or equal to 98 percent, the extraction rate is high, and the purity requirement on products in markets can be met.

The invention provides a method for separating and purifying chenodeoxycholic acid from duck gall, and concretely comprises to the following steps: dissolving and extracting a duck gall paste crude product, extracting ethyl acetate, and ultrafiltering and removing impurities. The method has the following advantages that 1) the raw material source of chenodeoxycholic acid can be enlarged, the method for separating and purifying chenodeoxycholic acid from the duck gall is provided; and 2) the content of chenodeoxycholic acid extracted from the duck gall by a traditional method is low with about 60%. The HPLC content of chenodeoxycholic acid obtained by using the method of the invention can reach more than 96%, and the extraction efficiency of chenodeoxycholic acid is enhanced.

The invention relates to a chenodeoxycholic acid (3 alpha, 7 alpha-dihydroxyl-5 belta-cholestane-24-acid) chemical synthesis method, belonging to the field of organic chemical synthesis. Chenodeoxycholic acid is prepared by the following steps of: (1) preparing cholate; (2) preparing 3 alpha, 7 alpha-acetyl-12 alpha-hydroxy cholate; (3) preparing 3 alpha, 7 alpha-acetyl-12-oxo-chenodeoxycholic acid ester; (4) preparing 12-oxo-chenodeoxycholic acid; and (5) preparing chenodeoxycholic acid. The application of the method for synthesizing chenodeoxycholic acid has the advantages of high yield rate, low cost and no pollution and is particularly convenient for industrial production.

A method for extracting chenodeoxycholic acid from bile of fowl comprises the following steps of: acquiring rough bile acid or rough bile acid calcium salt from fresh or frozen bile of fowl through saponification reaction, heating the bile acid resin solution together with the aqueous solution of organic nitrogenous compound A, removing the majority of hydrophilic impurities from the rough bile acid by using the organic nitrogenous compound A, reacting the acquired bile acid resin solution with organic nitrogenous compound B, dissolving the sediment, filtering, decolorizing and refining, so as to acquire chenodeoxycholic acid with a purity about 95%. The method of the invention has simple operation, the generality of the production equipment is high, and the chemic matter is harmless, therefore the invention is fit for industrialization of chenodeoxycholic acid.

The invention discloses a synthetic method of 6-ethylchenodeoxycholic acid. The synthetic method comprises steps as follows: chenodeoxycholic acid and an oxidizing agent are subjected to an oxidizing reaction and an esterification reaction, and a compound with a structure represented as a formula III is prepared; hydroxyl and carbonyl on rings of the compound with the structure represented as the formula III are protected with tert-butyldimethylsilyl chloride, and a compound with a structure represented as a formula IV is obtained; the compound with the structure represented as the formula IV and paraldehydeare are subjected to an electrophilic addition reaction and then are subjected to deprotection, and a compound with a structure represented as a formula V is obtained; the compound with the structure represented as the formula V is subjected to catalytic hydrogenation and is subjected to reduction and hydrolysis finally, and the compound 6-ethylchenodeoxycholic acid with a structure represented as a formula VI is obtained. The method is simple and convenient to operate, adopts mild conditions, has higher yield and is suitable for being popularized to industrial production.

A process for synthesizing chenodeoxycholic acid includes such steps as preparing the ester used for cholic acid, preparing diacetyl-12a-hydroxy-methyl cholate, preparing 3a, 7a-diacetyloxy-12-oxy-methyl cholanic acid, preparing 12-oxy- chenodeoxycholic acid, preparing chenodeoxycholic acid, and purifying.

The invention discloses a new method for preparing 3 alpha, 7 alpha-dihydroxy-6 alpha-alkyl-5 beta-cholanic acid I and an intermediate thereof. The method requires no low-temperature reaction; reaction conditions are mild; a purification method is simple; and the method of the invention is suitable for industrial production.

The invention discloses a method for preparing an ursodesoxycholic acid by the chiral catalytic hydrogenation of a 7-ketodesoxycholic acid, which is characterized by comprising the following steps of: performing oxidation to prepare the 7-ketodesoxycholic acid from a chenodeoxycholic acid serving as an initiative raw material by using a common method; dissolving the 7-ketodesoxycholic acid into a solvent, adding a chiral catalyst, maintaining the pressure of 0 to 20 MPa under alkali condition, introducing nitrogen to perform hydrogenation reduction reaction at 10 to 80 DEG C, and performing distillation after the reaction is finished to remove the solvent; adding purified water in a volume which is 10 to 100 times that of a hydrogenation reduction reaction product, and adding acid liquor to crystallize the hydrogenation reduction reaction product; and separating solids from liquid, and performing washing and drying to obtain solid powder which is the ursodesoxycholic acid. The method for preparing the ursodesoxycholic acid by the chiral catalytic hydrogenation of the 7-ketodesoxycholic acid aims to overcome the shortcomings of the prior art, and ensures a short production flow, high yield and high quality.

The invention relates to a method for respectively recovering ursodesoxycholic acid and chenodeoxycholic acid from ursodesoxycholic acid waste mother liquor. Firstly waste mother liquor is dissolved in an inorganic base solution, and inorganic acid is added to form mixed sediment containing the ursodesoxycholic acid and the chenodeoxycholic acid; secondly, an organic solvent and an organic amine are added to separate ursodesoxycholic acid ammonium salt; and thirdly the ursodesoxycholic acid ammonium salt is acidized to recover the ursodesoxycholic acid, chenodeoxycholic acid ammonium salt left in the organic solvent is acidized and filtered to recover the chenodeoxycholic acid. The recovering method can effectively utilize the ursodesoxycholic acid waste mother liquor, recovers the ursodesoxycholic acid and the chenodeoxycholic acid contained in the ursodesoxycholic acid waste mother liquor, and is high in recover efficiency and product purity. The recovering method is simple to operate and mild in reaction conditions, used reagent is low in cost and wide in source, and the method is suitable for large-scale industrialized production.

The invention discloses a 7beta-hydroxysterol dehydrogenase mutant with increased activity and stability which is obtained through molecular evolution, recombinant expression plasmid containing the 7beta-hydroxysterol dehydrogenase mutant gene and a recombinant expression transformant and a preparation method of a recombinant mutant enzyme preparation, and the invention also provides an application of the recombinant mutant enzyme preparation in ursodeoxycholic acid synthesis. The 7beta-hydroxysterol dehydrogenase has excellent activity and heat stability, can efficiently catalyze asymmetric reduction of 7-carbonyl lithocholic acid to prepare the ursodeoxycholic acid; the 7beta-hydroxysterol dehydrogenase is subjected to immobilization and then is subjected to couple by an enzyme method with the immobilized 7beta-hydroxysterol dehydrogenase, epimerization of a substrate chenodeoxycholic acid with low cost can be directly catalyzed, ursodeoxycholic acid can be prepared through continuous conversion, and the operation is simple. Compared with the prior art reported currently, ursodeoxycholic acid prepared by hydroxysterol dehydrogenase through catalysis has the advantages of high substrate concentration, short reaction time, complete reaction, and high product purity, and has strong industrial application prospect.

The invention relates to a set of biomarkers for diagnosing AD in a subject or determining a risk of the AD in the subject. Diagnostic markers are a cholic acid, a chenodeoxycholic acid, an allocholicacid, a benzpyrole-3-lactic acid and tryptophan. The invention also provides the application of the above diagnostic markers in preparation of a differential diagnostic reagent for an Alzheimer's disease and a kit. Plasma fingerprint spectrum analysis of AD patients, MCI patients, Ctrl and NDC populations is performed through a plasma sample collection-plasma sample pretreatment-ultra-high performance liquid chromatography and mass spectrometry analysis method, and contents of the above five diagnostic markers are detected so that the markers are applied to preparation of products related todiagnosis of the Alzheimer's disease, and therapeutic evaluation. The markers can evaluate an early phase of the Alzheimer's disease, accuracy is high, a detection speed is fast, cost is low, traumasare small, and patients can easily accept. A scientific and effective treatment plan is provided for the Alzheimer's disease and a good application prospect is possessed.

The invention discloses a method for synthesizing ursodeoxycholic acid through an enzymatic method and a method for synthesizing the ursodeoxycholic acid by taking chenodeoxycholic acid as a raw material. The method takes the chenodeoxycholic acid as a base material and comprises the following steps: dissolving the chenodeoxycholic acid into a 50mM phosphate buffer solution; firstly, catalyticallyoxidizing the chenodeoxycholic acid by utilizing 7-alpha hydroxysteroid dehydrogenase in the presence of NAD, NOX2 and under the condition that oxygen is introduced, so as to obtain 7-ketolithocholicacid; then under the condition that the NAD, L-malic acid and malic dehydrogenase exist, catalytically reducing the 7-ketolithocholic acid by utilizing the 7-alpha hydroxysteroid dehydrogenase to obtain the ursodeoxycholic acid. According to the method disclosed by the invention, an organic solvent is not used and operation is simple; reaction conditions are moderate and easy to control and the utilization rate of the raw materials is high; the conversion rate reaches 99 percent or more.

The invention discloses a method for extracting chenodeoxycholic acid and allocholic acid from duck bile, and belongs to the technical field of biological engineering. The method comprises the following steps of (1) preparing of bile acid extracting liquid: obtaining duck bile, saponifying, cooling, adjusting a pH (potential of hydrogen) value to 7 to 8, adding a mixed solvent of ester and alkane, adjusting the pH value to 2 to 4, extracting, and discoloring, so as to obtain the bile acid extracting liquid; (2) preparing of bile acid magnesium salt: adjusting the pH value of the bile acid extracting liquid in step (1) to 4.5 to 5.5, dissolving the magnesium salt, adding into the bile acid extracting liquid, refluxing, cooling, and separating, so as to respectively obtain the magnesium allocholate and a chenodeoxycholic acid solution; refining and purifying the chenodeoxycholic acid solution, so as to obtain a finished product of henodeoxycholic acid; (3) extracting of the allocholic acid: adding water and carbonate into the prepared magnesium allocholate in step (2), heating and dissolving, separating solid from liquid, adjusting the pH value of a solution to 1 to 2, and separating solid from liquid, so as to obtain a finished product of the allocholic acid. The method has the advantages that the purities and extracting rates of the chenodeoxycholic acid and the allocholic acid are greatly improved, the operation is safe, and the method is suitable for industrialization.