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Method for preparing cheodexycholic acid

A technology of chenodeoxycholic acid and cholestane acid, applied in the field of preparation of chenodeoxycholic acid, can solve the problems of high cost and low purity of CDCA

Inactive Publication Date: 2004-09-15
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] CDCA is mainly extracted from the bile of poultry or animal bile. The past extraction method involved toxic chemicals (animal biochemical pharmacy, 1981, People's Health Publishing House, P259; Pharmaceutical Industry, 1987, 18 (2): 75-76; ) or use a large amount of unsafe organic solvents (Chinese Journal of Biochemical Medicine, 1996, 17 (1): 17; Applied Technology, 1998, 4: 9-10; US Patent, 3,965,131; US ​​Patent, 4,331,607; US Patent, 4,163,017), cannot Adapt to the requirements of modern industry, and the prepared CDCA has low purity and high cost

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0017] Take fresh or frozen duck bile, goose bile or other poultry bile, add industrial sodium hydroxide to 2mol / L according to its volume, heat and boil for 5 hours for saponification, add CaCl according to 15% of bile volume 2 Stir for 30 minutes, centrifuge and collect the precipitate.

[0018] Suspend the filter cake in 8 times the amount of water, add hydrogen peroxide to make the concentration reach 1% of the suspension volume, then add solid sodium carbonate according to 10% of the weight of the bile to transform the precipitate, filter, collect the filtrate, and use a small amount of Wash with water, combine the filtrate and washing solution, add hydrochloric acid to pH 3, stir for 30 minutes, let stand, fully precipitate, centrifuge, collect the filter cake, and obtain crude chenodeoxycholic acid. Suspend the crude product with 5 times of water according to its wet weight, adjust to dissolve the solid matter with NaOH, add anhydrous calcium chloride according to the w...

Embodiment 2

[0021] Take the animal bile of pigs, cattle and sheep, extract bilirubin according to the bilirubin extraction method, add water to suspend the residue after bilirubin extraction according to the wet weight 1:4, and carry out saponification according to the same method as poultry bile, and then gather exactly the same. Chenodeoxycholic acid with a purity of about 93% can be obtained.

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PUM

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Abstract

The invention relates to an industrialized preparing method of extracting and purifying chenodeoxycholic acid from fowl and animal biles. Its process includes the steps of saponifying bile by lye, preparing calcium salt deposit of total bile acid, decolorizing by oxydol and active carbon, converting calcium salt deposit of chenodeoxycholic acid by sodium carbonate and HCl, purifying by the marcoporous absorbing resin, and so on. It has advantages of friend environment, safety and innocuity, low cost and be easy for large-scale operation.

Description

technical field [0001] The invention relates to a preparation method of chenodeoxycholic acid (3α, 7α-dihydroxy-5β-cholestane acid), in particular to a method for extracting chenodeoxycholic acid from the bile of poultry or animals. Background technique [0002] Chenodeoxycholic acid (3α, 7α-dihydroxy-5β-cholestanoic acid) Chenodeoxycholic Acid (CDCA for short), is a drug for the treatment of gallstones. It was first discovered in goose bile in 1848 and named as chenodeoxycholic acid in 1924. By reducing the absorption and synthesis of cholesterol, it reduces the cholesterol in the bile, thereby inhibiting the formation of cholesterol stones and promoting their dissolution, and can effectively reduce the saturation of cholesterol. [0003] In addition to its medicinal use, chenodeoxycholic acid can also be used as a raw material for the preparation of ursodeoxycholic acid (3α, 7β-dihydroxy-5β-cholic acid, referred to as UDCA). Ursodeoxycholic acid is the main active ingred...

Claims

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Application Information

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IPC IPC(8): C07J9/00
Inventor 曹学君
Owner EAST CHINA UNIV OF SCI & TECH
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