Method for purifying chenodeoxycholic acid

Abstract

The invention discloses a method for purifying chenodeoxycholic acid. The method comprises the following steps: pretreatments such as degreasing, decoloration and impurity removal are carried out on commercially available crude bile paste or crude bile powder, so as to obtain a component to undergo chromatographic separation; the component to undergo chromatographic separation undergoes purification and separation by the use of a chromatographic column with a hydrophilic resin filler as a stationary phase; and separation products undergo concentration, acidification, washing and drying, so as to obtain chenodeoxycholic acid. In comparison with the prior art, the invention has the following beneficial effects: 1, content of the main component is high; 2, reappearance is high and operability is good; 3, short cycle: it only takes two days to prepare the high-purity chenodeoxycholic acid product from processing of the bile paste raw material; and 4, low content of organic residues: as processes such as solvent extraction, column chromatography on silica gel and the like in the traditional techniques are abandoned, an industrial chromatographic process with low dosage of an organic solvent is adopted and the final product undergoes drying process, the content of the residual organic solvent in the final product is especially low.

Description

technical field [0001] The invention relates to a method for purifying chenodeoxycholic acid, which belongs to the technical field of separation and purification of biomedicine. Background technique [0002] Chenodeoxycholic acid (CDCA for short), chemical name 3α, 7α-dihydroxy-5β-cholic acid, molecular formula C 24 h 4 0O 4 , with a molecular weight of 392.58, is currently one of the most widely used medicines for treating gallstones in the world, and is also the raw material for the synthesis of ursodeoxycholic acid (UDCA) and other steroidal compounds. In industry, chenodeoxycholic acid is mainly extracted from animal bile come. [0003] The current literature reports on the method of separating chenodeoxycholic acid from animal bile, focusing on solvent extraction, precipitation, silica gel chromatography, ion exchange resin method, macroporous resin method and other methods, such as: Chinese patent CN1775798 adopted Ion exchange method, what Chinese patent CN10114...

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Problems solved by technology

[0003] Current literature reports on methods for separating chenodeoxycholic acid from animal bile, focusing on solvent extraction, precipitation, silica gel chromatography, and ion exchange resin methods , macroporous resin method and other methods, such as: what Chinese patent CN1775798 adopted was ion exchange method, what Chinese patent CN101143886, CN103113447A and Chinese patent CN101948496A adopted was precipitation method, what Chinese patent CN 102703556A adopted was macroporous resin method etc., these methods There are disadvantages such as complicated separation process, low yield, low product purity, poor reproducibility, long time consumption, serious organic residues, and incapability of large-scale production.