Method for extracting chenodeoxycholic acid and allocholic acid from duck bile

A technology of chenodeoxycholic acid and allocholic acid, which is applied in the field of bioengineering, can solve the problems of difficult industrial application, complex process, low yield, etc., and achieve the advantages of improving operational safety, increasing extraction rate, and high boiling point Effect

Active Publication Date: 2017-05-31
CHANGDE YUNGANG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] So far, the traditional process of extracting chenodeoxycholic acid and allocholic acid from c

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for extracting chenodeoxycholic acid and allocholic acid from duck bile, comprising the steps of:

[0029] (1) prepare bile acid extract: get duck bile 80kg, saponify, get saponified liquid, add sodium hydroxide in the saponified liquid, make the mass percent concentration of sodium hydroxide reach 14.5%, room temperature is cooled to educt and float on the surface of saponified liquid , separate and collect the lower saponified solution to obtain saponified precipitates. Add 8kg of water to the saponification precipitate, after heating to dissolve, add dilute hydrochloric acid while stirring to adjust the pH value to 7.5, add 60kg of mixed solvent of butyl acetate and dichloroethane, wherein the mass of butyl acetate and dichloroethane The ratio is 8:2, then add dilute hydrochloric acid to adjust the pH to 3 while stirring, add 0.3kg of activated carbon for decolorization after stirring and extraction, heat to reflux for 120min, cool, press filter, and separat...

Embodiment 2

[0034] A method for extracting chenodeoxycholic acid and allocholic acid from duck bile, comprising the steps of:

[0035] (1) prepare bile acid extract: get duck bile 100kg, saponify, get saponified liquid, add sodium hydroxide in the saponified liquid, make the mass percent concentration of sodium hydroxide reach 15.2%, cool at room temperature until precipitate floats on the surface of saponified liquid , separate and collect the lower saponified solution to obtain saponified precipitates. Add 15kg water in the saponification precipitate, after heating and dissolving, add dilute hydrochloric acid while stirring to adjust the pH value to 8, add the mixed solvent of 40kg butyl acetate and n-heptane, wherein the mass ratio of butyl acetate and n-heptane is 7: 3. Then add dilute hydrochloric acid while stirring to adjust the pH value to 2. After stirring and extracting completely, add 0.5 kg of activated carbon for decolorization, heat to reflux for 60 minutes, cool, press filt...

Embodiment 3

[0040] A method for extracting chenodeoxycholic acid and allocholic acid from duck bile, comprising the steps of:

[0041] (1) prepare bile acid extract: get duck bile 100kg, saponify, get saponified liquid, add sodium hydroxide in the saponified liquid, make the mass percent concentration of sodium hydroxide reach 14.8%, cool at room temperature until precipitate floats on the surface of saponified liquid , separate and collect the lower saponified solution to obtain saponified precipitates. Add 12kg of water to the saponification precipitate, after heating to dissolve, add dilute hydrochloric acid while stirring to adjust the pH value to 8, add 40kg of mixed solvent of ethyl propionate and cyclohexane, wherein the mass of ethyl propionate and cyclohexane The ratio is 8:2, then add dilute hydrochloric acid to adjust the pH value to 2 while stirring, add 0.5kg of activated carbon for decolorization after stirring and extraction, heat to reflux for 120min, cool, press filter, a...

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PUM

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Abstract

The invention discloses a method for extracting chenodeoxycholic acid and allocholic acid from duck bile, and belongs to the technical field of biological engineering. The method comprises the following steps of (1) preparing of bile acid extracting liquid: obtaining duck bile, saponifying, cooling, adjusting a pH (potential of hydrogen) value to 7 to 8, adding a mixed solvent of ester and alkane, adjusting the pH value to 2 to 4, extracting, and discoloring, so as to obtain the bile acid extracting liquid; (2) preparing of bile acid magnesium salt: adjusting the pH value of the bile acid extracting liquid in step (1) to 4.5 to 5.5, dissolving the magnesium salt, adding into the bile acid extracting liquid, refluxing, cooling, and separating, so as to respectively obtain the magnesium allocholate and a chenodeoxycholic acid solution; refining and purifying the chenodeoxycholic acid solution, so as to obtain a finished product of henodeoxycholic acid; (3) extracting of the allocholic acid: adding water and carbonate into the prepared magnesium allocholate in step (2), heating and dissolving, separating solid from liquid, adjusting the pH value of a solution to 1 to 2, and separating solid from liquid, so as to obtain a finished product of the allocholic acid. The method has the advantages that the purities and extracting rates of the chenodeoxycholic acid and the allocholic acid are greatly improved, the operation is safe, and the method is suitable for industrialization.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a method for extracting chenodeoxycholic acid and allocholic acid from duck bile. Background technique [0002] Chenodeoxycholic acid in bile acids has the effect of reducing the saturation of cholesterol in bile, thereby having a good therapeutic effect on gallbladder stone disease; and chenodeoxycholic acid is the main raw material for preparing ursodeoxycholic acid, and it is now on the market Facing the situation of continuous and rapid growth of ursodeoxycholic acid, the supply of chenodeoxycholic acid has become a serious bottleneck. Allocholic acid is a good feed additive ingredient. With the vigorous promotion of animal husbandry in my country, the use of feed additives will be steadily and rapidly increased. The content of chenodeoxycholic acid in duck bile is 1% to 1.2%, and the content of allocholic acid is 0.9% to 1.1%. [0003] So far, the traditional proces...

Claims

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Application Information

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IPC IPC(8): C07J9/00
CPCC07J9/005
Inventor 邓家国李国军
Owner CHANGDE YUNGANG BIOTECHNOLOGY CO LTD
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