The invention discloses high-entropy alloy powder for laser cladding and a preparation method of a cladding layer. The high-entropy alloy powder comprises Ti, Zr, V, Nb, Ta and Mo. In addition, high-entropy alloy powder which comprises Ti, Zr, V, Nb, Ta, Mo and W, high-entropy alloy powder which comprises Ti, Zr, V, Nb, Ta, Mo, W and Cr and the preparation method of the cladding layer of the high-entropy alloy powder for laser cladding are provided. The high-entropy alloy powder mainly comprises two characteristics, namely, the high-entropy effect and the cocktail effect, each kind of powder exerts respective advantages, and therefore the cladding layer with high hardness, high corrosion resistance, excellent tempering softening resisting performance and excellent structure performance is obtained. Metallurgical bonding is formed by an alloy layer and a matrix through laser cladding; the bonding strength of the cladding layer and the matrix is greatly improved, and the heating speed is high; metal of the cladding layer is not prone to being diluted by the matrix; little heat deformation is generated; and therefore the part rejection rate is low.