43 results about "Sugar phosphates" patented technology

Oligonucleotides with a novel sugar-phosphate backbone containing at least one 2′-arabino-fluoronucleoside and an internucleoside 3′-NH—P(—O)(OR)—O-5′ linkage, where R is a positively charged counter ion or hydrogen, and methods of synthesizing and using the inventive oligonucleotides are provided. The inventive phosphoramidate 2′-arabino-fluorooligonucleotides have a high RNA binding affinity to complementary nucleic acids and are base and acid stable.

Oligonucleotides with a novel sugar-phosphate backbone containing at least one internucleoside 3′-NHP(O)(S⁻)O-5′ linkage, and methods of synthesizing and using the inventive oligonucleotides are provided. The inventive thiophosphoramidate oligonucleotides were found to retain the high RNA binding affinity of the parent oligonucleotide N3′→P5′ phosphoramidates and to exhibit a much higher acid stability.

The invention relates to a small interfering nucleic acid and a pharmaceutical composition and an application thereof. The siRNA contains complementary positive-sense strand and antisense strand, wherein the positive-sense strand contains nucleotide sequences as shown in SEQ ID NO.24, SEQ ID NO.26, SEQ ID NO.28 or SEQ ID NO.30, and the antisense strand contains nucleotide sequences as shown in SEQID NO.25, SEQ ID NO.27, SEQ ID NO.29 or SEQ ID NO.31. A phosphoric acid framework and/or nucleotide pentose in a phosphoric acid-sugar framework of the siRNA has or does not have modifying groups. The invention provides a brand new efficient siRNA and a pharmaceutical composition thereof, and the siRNA can prevent and/or treat dyslipidemia effectively.

The invention discloses a soybean fermentation metabolin. Components of the metabolin comprise amino acid and a derivative thereof, amine, sugar phosphate, nucleoside, a nucleoside derivative, organicacid and fatty acid. In addition, the invention further discloses a preparation method and application of the soybean fermentation metabolin. The soybean fermentation metabolin has the remarkable effects of improving diversity and quality of skin bacterial flora, skin water content and skin acid mantle, and is capable of avoiding loss of skin beneficial substances, maintaining balance of the skinbacterial flora, reinforcing nonspecific immune response, preventing skin infection and inflammation, and preventing skin related diseases, such as pruritus, acne, scurf, rubeosis and drying.

A method for producing a pyrimidine nucleoside compound includes reacting a sugar phosphate and pyrimidine base derivative using an enzyme having cytosine nucleoside phosphorylase activity, the pyrimidine base derivative being represented by general formula (I): (wherein R1 represents an amino group that may be replaced with an acyl group having an alkyl group of 1 to 3 carbon atoms or an alkyl group of 1 to 3 carbon atoms, an alkyl group of 1 to 3 carbon atoms, or a thiol group; R2 represents an amino group, a thiol group, a hydroxyl group, or a hydrogen atom; R3 represents an alkyl group of 1 to 3 carbon atoms that may be replaced with a hydroxyl group, an amino group, or a hydrogen atom; R4 represents a hydroxyl group or a hydrogen atom; when R1 is an amino group, when R2 is a hydroxyl group, and when R4 is a hydrogen atom; R3 is neither an alkyl group of 1 to 3 carbon atoms nor a hydrogen atom).

A refolding agent and refolding method which make it possible to produce high-purity proteins in high productivity. The refolding agent includes a phosphorus-containing compound (A) and an oxycarbonyl group-containing compound (B). The refolding method includes the step of treating the unfolded protein with the refolding agent. As the compound (A), there may be mentioned at least one species selected from inorganic phosphoric acids, alkyl phosphate esters, sugar phosphate esters, and salts of these, and as the compound (B), there may be mentioned at least one species selected from formic acid, acetic acid, propionic acid, lactic acid, tartaric acid, and salts of these.

The invention belongs to the technical field of inorganic materials and analysis and particularly relates to a pretreatment method for enrichment and solid-phase derivatization of sugar phosphate substances by a metal ion affinity chromatography material (SiO2@PD-Ti<4+>). The pretreatment method comprises the following steps: firstly, enriching the sugar phosphate substances on SiO2@PD-Ti<4+> microspheres by a metal chelating action; secondly, carrying out solid-phase derivatization on enriched sugar phosphate substances. According to the method, retention and separation degree of the sugar phosphate substances on reversed phase liquid chromatography can be improved, and the mass spectrometric detection sensitivity of the sugar phosphate substances with low abundance can be improved. The pretreatment method disclosed by the invention has the advantages of simple and quick pretreatment process, good repeatability, low detection limit and the like.

The invention discloses an analysis method for a variety of sugar phosphate compounds including endogenous trehalose-6-phosphate in a plant sample. The method comprises the following steps of: extracting the endogenous sugar phosphates through a solvent; adsorbing and removing the hydrophobic impurities in the extracted solution through a reversed-phase SPE column; using the diazo groups in a derivatization reagent to carry out derivatization reaction on the phosphate groups common to the sugar phosphate compounds; and in combination with the liquid chromatography and mass spectrometry, realizing the separation detection of the endogenous sugar phosphate isomers in the plant sample. The analysis method is simple, accurate and high in throughput, can achieve the baseline separation of the sugar phosphate isomers on a hydrophilic chromatographic column and guarantee the higher detection sensitivity, and finally can realize the separation detection of the endogenous sugar phosphates in amicroplant tissue sample.

Disclosed are genetically engineered microbial cells for the production of a carbohydrate of interest, wherein the microbial cells possess an increased intracellular availability of at least one sugar phosphate, and produce the carbohydrate of interest when being cultivated in a culture medium containing a mixed monosaccharide feedstock as main carbon and energy source. Also disclosed is a fermentative method of producing a carbohydrate of interest by cultivating said genetically engineered microbial cell in the presence of a mixed monosaccharide feedstock as main carbon and energy source.

A composition comprising at least two glycoalkaloids of formula I:

wherein: either one or both of the dotted lines represents a double bond, and the other a single bond, or both represent single bonds;

A: represents a radical selected from the following radicals of general formulae (II) to (V):

• • each of R¹ is a radical separately selected from the group consisting of hydrogen, amino, oxo and OR⁴;
  • each of R² is a radical separately selected from the group consisting of hydrogen, amino and OR⁴;
  • each of R³ is a radical separately selected from the group consisting of hydrogen, carbohydrate and a carbohydrate derivative;
  • “X” is a radical selected from the group comprising —CH₂—, —O— and —NH₂—; and
  • wherein the compound includes at least one R⁴ group that is a carbohydrate or a derivative thereof selected from the group comprising glyceric aldehyde, glycerose, erythrose, threose, ribose, arabinose, xylose, lyxose, altrose, allose, gulose, mannose, glucose, idose, galactose, talose, rhamnose, dihydroxyactone, erythrulose, ribulose, xylulose, psicose, fructose, sorbose, tagatose, and other hexoses, heptoses, octoses, nanoses, decoses, deoxysugars with branched chains, (e.g. apiose, hamamelose, streptose, cordycepose, mycarose and cladinose), compounds wherein the aldehyde, ketone or hydroxyl groups have been substituted (e.g. N-acetyl, acetyl, methyl, replacement of CH₂OH), sugar alcohols, sugar acids, benzimidazoles, the enol salts of the carbohydrates, saccharinic acids, sugar phosphates; wherein the ratio of said glycoalkaloids is between 6:1 and 1:6 and on the proviso that when the glycoalkaloids are solamargine and solasonine and they are present in a 1:1 ratio the solamargine and solasonine are isolated.

The invention relates to methods for the treatment of tumors and/or for immune suppression and/or sepsis by modulating the association of the glycolysis enzyme complex/M2-PK and/or by inhibition of transaminases and/or separation of the binding of the malate dehydrogenase to p36 comprising administering a pharmaceutical composition comprising a substance selected from the group consisting of amino acids, amino acid analogs, sugar phosphates, sugar phosphate analogs, and mixtures of said substances.

The invention belongs to the field of chemical synthesis, and relates to a synthesis method of nucleoside diphosphate saccharide. The synthesis method comprises the following steps: 1) taking fully-protected nucleoside phosphoryl piperidine, removing all protecting groups through palladium carbon catalytic hydrogenation in an N,N-dimethylformamide or dimethylsulfoxide solution, and filtering to remove palladium and carbon to obtain corresponding deprotected nucleoside phosphoryl piperidine; 2) adding glycosyl-1-monophosphate and 4, 5-dicyanoimidazole (DCI) into the nucleoside phosphoryl piperidine, and reacting to obtain a nucleoside diphosphate saccharide product; 3) performing ethanol precipitation, reverse phase C18HPLC (high performance liquid chromatography) purification and final ion exchange on a crude product to obtain corresponding nucleoside diphosphate 6-deoxy-L-pyranose with high purity. By the synthesis method, the yield is high, the reaction time is short and the product separation is simple; therefore, the synthesis method has a high application value.

The invention relates to a novel internal standard substance for detecting amino acid, organic acid or sugar phosphate metabolite in a sample. The invention discloses a novel compound suitably used asthe internal standard substance during the detection process of the amino acid, organic acid or sugar phosphate metabolite in a to-be-detected sample, and builds a detection method. By the internal standard substance and the detection method, the detection accuracy of a fermentation metabolite can be effectively improved, and the internal standard substance is simple and convenient in process andrelatively low in cost.

Provided is a siRNA that inhibits RRM2 gene expression. A sense-strand base sequence of the siRNA is as shown in SEQ ID NO:2, an antisense-strand base sequence of the siRNA is as shown in SEQ ID NO:3, and a phosphate-sugar backbone of the siRNA may further have a modified group; also provided is a pharmaceutical composition comprising the siRNA, which can inhibit tumor cell proliferation and facilitate tumor cell apoptosis, thereby inhibiting growth of tumor tissues.