Novel internal standard for the detection of amino acids, organic acids, or sugar phosphate metabolites in samples

Abstract

The invention relates to a novel internal standard substance for detecting amino acid, organic acid or sugar phosphate metabolite in a sample. The invention discloses a novel compound suitably used asthe internal standard substance during the detection process of the amino acid, organic acid or sugar phosphate metabolite in a to-be-detected sample, and builds a detection method. By the internal standard substance and the detection method, the detection accuracy of a fermentation metabolite can be effectively improved, and the internal standard substance is simple and convenient in process andrelatively low in cost.

Description

technical field

[0001] The invention belongs to the field of substance detection, and more specifically, the invention relates to a novel internal standard for detecting amino acids, organic acids or phosphate sugar metabolites in samples. Background technique

[0002] In the fermentation industry, the detection of intracellular metabolites is generally required. Due to the characteristics of various types, low concentration and fast turnover of intermediate metabolites, gas chromatography-mass spectrometry (GC-MS) has been applied to the qualitative and quantitative analysis of these substances (GUOMeng-Lei, LIU Xiao-Yun, HUANG Ming-Zhi , et al. 13 C-assisted ultra-high performance liquid chromatography triple quadrupole mass spectrometry method for precise determination of intracellular metabolites in Pichia pastoris. Chinese J. Anal. Chem., 2016, 44(2):232-240). At present, the detection of intracellular metabolites mostly adopts the isotope dilution-mass spectrometry q...

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