Moisturizing face cream with rejuvenating effect and preparation method of moisturizing face cream

Abstract

The invention relates to the technical field of cosmetics, in particular to a moisturizing face cream with a red repairing effect and a preparation method thereof, the moisturizing face cream comprises the following components: an oil phase: artemisia apiacea volatile oil, camellia oil, a repairing nano-carrier, squalane, cetostearyl alcohol and ceramide; and a water phase: deionized water, glycerol, sodium polyglutamate, trehalose and lecithin. According to the repairing nano-carrier, emulsified particles containing repairing components are infiltrated into the carrier in a vacuum impregnation manner, so that the loaded repairing components can stay on the skin for a long time; through water-in-oil emulsified particles with repairing components, the repairing components are wrapped in micro-nanospheres of monoglyceride, and the slow-release effect of the repairing components is further achieved; effective rejuvenating components are carried and permeated into the skin to reach the inflammation position by utilizing glyceride, so that the antianaphylaxis is reduced, the inflammation is prevented, the skin barrier is comprehensively enhanced, couperose skin is effectively removed, and the redness phenomenon is improved.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, in particular to a moisturizing facial cream with redness-removing effect and a preparation method thereof. Background Art

[0002] Facial redness and facial flushing are common skin problems. Facial redness is caused by the weakness of the facial stratum corneum, which makes it easier for capillaries to contact and sense changes in the external environment. In turn, the blood vessels continue to expand unevenly or even rupture, causing the skin to flush, which causes facial flushing, often accompanied by red or purple-red spots, dots, lines or stars. The face with red blood streaks or flushing is a little redder, which is often called plateau face or red cheeks. Some have red cheeks on both sides, with round borders, generally arranged in silky lines. This kind of skin is thin and sensitive, and the face will become redder when it is too cold, too hot or emotional. In severe cases, it may even form deposited spots, which are difficult to cure.

[0003] There are many reasons for red blood streaks and facial redness, mainly congenital, of course there are also some acquired factors, such as improper beauty care, ultraviolet radiation, drug factors, disease factors and bad personal habits: liking spicy food, addiction to smoking and drinking, etc., causing cuticle damage, capillaries lose the natural protection of the cuticle, and thus long-term stimulation by the external environment, leading to blood vessel dilation, blockage, and damage. With the improvement of people's living standards, skin care awareness is getting stronger and stronger, and the treatment of facial capillary dilation has also received more attention.

[0004] Chinese patent CN202210351014.2 discloses an anti-inflammatory soothing composition based on targeted redness repair and its preparation method, comprising a dispersed phase, an active substance and a nanocarrier, wherein the nanocarrier is an emulsified particle dispersed in the dispersed phase, and the active substance is encapsulated in the emulsified particle. However, after the skin care composition acts on the skin, it cannot repair the skin for a long time, and thus cannot achieve the relief of facial redness and the reduction of red blood streaks in a short time.

[0005] Therefore, it is urgent to develop a good redness-repairing moisturizing cream to alleviate facial redness and reduce red blood streaks. Summary of the invention

[0006] In view of the deficiencies in the prior art, the present invention provides a moisturizing facial cream with redness-repairing effect and a preparation method thereof. The facial cream can prepare a repairing nano-carrier material with a sustained-release structure so that it can form an effective coating and retention on the surface of the skin, thereby prolonging the moisturizing and redness-repairing performance and duration, solving the problem that there are currently few skin care products for facial redness and redness, and the effects are average and the moisturizing property is poor.

[0007] To achieve the above purpose, the specific technical solutions of the present invention are as follows:

[0008] The invention provides a moisturizing facial cream with redness repairing effect, which comprises the following substances in parts by weight: an oil phase: 40-50 parts of artemisia annua volatile oil, 10-20 parts of camellia oil, 5-10 parts of repairing nano-carriers, 5-10 parts of squalane, 1-5 parts of cetearyl alcohol, and 1-2 parts of ceramide; and a water phase: 50-60 parts of deionized water, 15-20 parts of glycerol, 5-10 parts of sodium polyglutamate, 5-10 parts of trehalose, and 5-10 parts of lecithin.

[0009] The repair nano-carrier is prepared by loading emulsified particles containing repair ingredients with composite silk fibroin micro-nanospheres as a matrix and a carrier, wherein the emulsified particles are oil-in-water multi-structure emulsified particles; the multi-structure emulsified particles include an outer oil phase and an inner emulsified composition, wherein the outer oil phase includes 10-20 parts of monoglyceride, 5-10 parts of glyceryl stearate, 5-10 parts of olive oil, and 5-10 parts of horse oil in parts by weight; the inner emulsified composition containing the repair ingredient includes 20-25 parts of water, 10-20 parts of madecassoside, 5-10 parts of sodium heparin, 5-10 parts of paeonol, and 1-5 parts of bisabolol; the inner emulsified composition is wrapped by the outer oil phase.

[0010] The preparation method of the composite silk fibroin micro-nanospheres of the present invention is as follows:

[0011] (1) taking 40-60 mL of a disodium hydrogen phosphate aqueous solution, adjusting the pH to 6.0-6.8, dissolving 1.0-1.5 g of silk fibroin, 0.3-0.5 g of calcium nitrate and 0.5-1.0 g of sodium carbonate in the prepared disodium hydrogen phosphate aqueous solution, adding ethanol, and then stirring at 100-200 r / min for 3-5 min to obtain a pretreatment liquid;

[0012] (2) subjecting the pretreated liquid to low-temperature coagulation treatment, and then thawing, dialyzing, and diluting treatment, and then subjecting the pretreated liquid to wet heat crystallization treatment at a treatment temperature of 100 to 130° C. for 15 to 30 minutes to obtain a highly crystalline silk fibroin microsphere liquid, and spray drying the highly crystalline silk fibroin microsphere liquid to obtain solid silk fibroin micro-nanospheres;

[0013] (3) After repeatedly washing the solid silk fibroin micro-nanospheres with deionized water by centrifugation, the micro-nanospheres were transferred to 30-50 mL of a sodium dihydrogen phosphate aqueous solution, and then placed in a high-pressure reactor to react at 100-120° C. for 2-4 h. After naturally cooling to room temperature, the micro-nanospheres were washed with ethanol and deionized water in turn, and then freeze-dried to obtain silk fibroin micro-nanospheres;

[0014] (4) Sodium chloride aqueous solutions containing 1-5 mg / mL sodium polystyrene sulfonate, chitosan, and sodium hyaluronate were prepared respectively, and the pH values ​​of the solutions were adjusted to 6.0-6.8. Then, the silk fibroin micro-nanospheres were immersed in the sodium chloride aqueous solutions of sodium polystyrene sulfonate, chitosan, and sodium chloride aqueous solutions of sodium hyaluronate in turn for 15-25 min. After each immersion, the product was repeatedly centrifuged and washed with the sodium chloride aqueous solution. Then, the product was etched with an acetic acid aqueous solution for 3-5 times, each etching time for 2-3 h, and then centrifuged and washed with deionized water. After low-temperature coagulation treatment, freeze-drying was performed to obtain composite silk fibroin micro-nanospheres.

[0015] Furthermore, the concentration of the sodium hydrogen phosphate aqueous solution in step (1) and step (3) is 10 to 20 mg / mL.

[0016] Furthermore, the thawing temperature in step (2) is 10-30°C, the dilution multiple is 1-20 times; the molecular weight cutoff of the dialysis material for dialysis is 3500Da, and the time is 24-48h, wherein the dialysis water is replaced every 1-2h.

[0017] Furthermore, the concentration of the acetic acid aqueous solution in step (4) is 0.1 to 0.3 mol / L.

[0018] Furthermore, the low-temperature solidification treatment in steps (2) and (4) is freezing at a temperature of -80°C to -20°C for 8 to 24 hours.

[0019] Furthermore, in step (4), the concentration of the sodium chloride aqueous solution is 0.5-0.8 mol / L; the concentration of the acetic acid aqueous solution is 0.1-0.3 mol / L.

[0020] In the present invention, the preparation method of the repair nanocarrier is as follows:

[0021] S1: uniformly mix monoglyceride, glyceryl stearate, olive oil and horse oil to obtain an oil phase; uniformly mix water, madecassoside, sodium heparin, paeonol and bisabolol to obtain an emulsified composition; after mixing the oil phase and the aqueous phase of the emulsified composition, continue stirring for 30 to 50 minutes, and then mechanically shear for 1 to 3 minutes at a speed of 200 to 2000 rad / min to obtain an oil-in-water micron dispersion; finally, mechanically treat the oil-in-water micron dispersion at 200 to 2000 bar to obtain emulsified particles containing repair components;

[0022] S2: Place the composite silk fibroin micro-nanospheres in a vacuum impregnation tank, evacuate to 10-50Pa, maintain for 10-30 minutes, inject emulsified particles, continue to maintain for 1-2 hours, then slowly release the pressure to normal pressure, evacuate again to 1-5Pa, and maintain for 30-50 minutes. After the pressure is released to normal pressure, take out the product, drain the water and dry it naturally to obtain the repair nano-carrier.

[0023] Furthermore, the usage ratio of the composite silk fibroin micro-nanospheres to the emulsified particles is (5-10) g: (20-30) mL.

[0024] In the present invention, silk fibroin and calcium carbonate microspheres are used as templates, and a hydrothermal method is used to synthesize silk fibroin micro-nanospheres on the surface thereof; then, natural polyelectrolyte chitosan and sodium hyaluronate are assembled on the surface of the silk fibroin micro-nanospheres by electrostatic action; and then, the calcium carbonate template is etched away by glacial acetic acid, thereby obtaining silk fibroin micro-nanospheres with a porous structure. The silk fibroin micro-nanospheres can be used as carriers to load emulsified particles containing repair components, so that the repair components in the composition are coated and exist in the form of slow-release micro-nanospheres, and the polyelectrolyte multilayer film formed by electrostatic self-assembly on the surface of the micro-nanospheres can further achieve a slow-release effect, prolong the action time of the repair components, thereby helping to improve the utilization rate of the repair liquid, and the outer multilayer film structure can support and protect the silk fibroin micro-nanospheres, and the functional groups such as -COOH, NH2, and -OH contained in the silk fibroin can form hydrogen bonds with the chitosan of the multilayer film structure, so that a firm structure is formed between the multilayer film structure and the silk fibroin micro-nanospheres, thereby helping to increase the mechanical strength of the silk fibroin micro-nanospheres, so that it has good structural stability.

[0025] In the present invention, silk protein micro-nanospheres are used as carriers, and the emulsified particles containing repair components are infiltrated into the carrier by vacuum impregnation to form repair nano-carriers. Madecassoside, sodium heparin, paeonol, and bisabolol contained in the emulsified particles have anti-inflammatory, antibacterial, moisturizing, and antioxidant effects, and can reduce the degree of damage to the skin barrier function, prevent inflammation from occurring, and enhance the skin barrier in all directions, thereby improving the skin defense capability; for the red blood streaks and reddening phenomena that appear on the skin, the red blood streaks can be effectively removed and the reddening phenomenon can be improved; monoglyceride, glyceryl stearate, olive oil, and horse oil can seal the skin and form a protective film, which can prevent the moisture in the damaged part of the skin from radiating outward, thereby effectively reducing capillary activity, promoting cell and epithelial tissue damage relief, thereby accelerating the repair process of the skin. Moreover, due to the effective hydrophilicity of functional groups such as -COOH, NH2, and -OH in silk protein, the water retention performance of silk protein can be improved, and the silk protein can also form hydrogen bonds and other forces with the effective components of the repair nano-carrier, which can further achieve a sustained release effect on the repair component.

[0026] In order to make the repairing ingredients better penetrate into the skin epidermis, the present invention prepares oil-in-water emulsified particles to encapsulate madecassoside and active ingredients in micro-nanospheres of monoglyceride, and utilizes the good skin permeability of glyceride to carry madecassoside sodium heparin, paeonol, bisabolol, etc. into the skin, thereby enabling the repairing ingredients to reach the lesions where inflammation occurs.

[0027] The preparation method of a moisturizing facial cream with redness-removing effect comprises the following preparation steps:

[0028] S1': weigh 40-50 parts of Artemisia annua volatile oil, 10-20 parts of camellia oil, 5-10 parts of repair nanocarriers, 5-10 parts of squalane, 1-5 parts of cetearyl alcohol, and 1-2 parts of ceramide respectively by weight;

[0029] S2': weigh 50-60 parts of deionized water, 15-20 parts of glycerol, 5-10 parts of sodium polyglutamate, 5-10 parts of trehalose, and 5-10 parts of lecithin respectively by weight to obtain an aqueous phase;

[0030] S3': Slowly add the oil phase to the water phase at a mass ratio of 1:5, stir and mix, and homogenize to obtain a homogenous emulsion. After standing to degas, sterilize with ultraviolet light to prepare a moisturizing cream with redness-removing effect.

[0031] Furthermore, the deceleration rate in step S3' is 5 mL / min.

[0032] The moisturizing facial cream with redness repairing effect of the present invention can stay on the skin for a long time, so that the loaded repairing ingredients can effectively penetrate into the skin, which can better repair the skin, promote the metabolism of cells and epithelial tissues, reduce allergies and prevent inflammation, and comprehensively enhance the skin barrier; at the same time, it can also effectively reduce capillary activity, effectively remove red blood streaks and redness on the skin, and improve the redness phenomenon.

[0033] The beneficial effects of the present invention are:

[0034] The present application provides a moisturizing facial cream with redness repairing effect and a preparation method thereof. Silk fibroin micro-nanospheres are used as carriers, and emulsified particles containing repairing ingredients are infiltrated into the carriers by vacuum impregnation to obtain repairing nano-carriers, so that the loaded repairing ingredients such as madecassoside, sodium heparin, paeonol, and bisabolol can stay on the skin for a long time to better repair the skin; then, a suitable emulsifier ratio and a high-pressure homogenization method are used to obtain oil-in-water multi-structured emulsified particles, so that the effective repairing ingredients are wrapped in the micro-nanospheres of monoglyceride, further achieving the sustained-release effect of the repairing ingredients, prolonging the action time of the repairing ingredients and improving the effect; and by utilizing the good skin permeability of glyceride, madecassoside, sodium heparin, paeonol, bisabolol, etc. are carried and infiltrated into the skin, so that the repairing ingredients can reach the lesions where inflammation occurs, reduce anti-allergy and prevent inflammation, enhance the skin barrier in all directions, effectively remove red blood streaks, and improve the redness of the skin. DETAILED DESCRIPTION

[0035] The present invention is further described below in conjunction with the examples, but the present invention is not limited thereby. The experimental methods in the following examples are conventional methods unless otherwise specified.

[0036] Example 1

[0037] A moisturizing facial cream with redness repairing effect comprises the following substances in parts by weight: an oil phase: 40 parts of artemisia annua volatile oil, 10 parts of camellia oil, 5 parts of repairing nano-carriers, 5 parts of squalane, 1 part of cetearyl alcohol and 1 part of ceramide; an aqueous phase: 50 parts of deionized water, 15 parts of glycerol, 5 parts of sodium polyglutamate, 5 parts of trehalose and 5 parts of lecithin.

[0038] The repair nano-carrier is prepared by loading emulsified particles containing repair ingredients with composite silk fibroin micro-nanospheres as a matrix and a carrier, wherein the emulsified particles are oil-in-water multi-structure emulsified particles; the multi-structure emulsified particles include an outer oil phase and an inner emulsified composition, and the outer oil phase includes 10 parts of monoglyceride, 5 parts of glyceryl stearate, 5 parts of olive oil, and 5 parts of horse oil in parts by weight; the inner emulsified composition containing the repair ingredient includes 20 parts of water, 10 parts of madecassoside, 5 parts of sodium heparin, 5 parts of paeonol, and 1 part of bisabolol.

[0039] The preparation method of the repair nanocarrier is as follows:

[0040] (1) taking 40 mL of a 10 mg / mL sodium hydrogen phosphate aqueous solution, adjusting the pH to 6.5, dissolving 1.0 g of silk fibroin, 0.3 g of calcium nitrate and 0.5 g of sodium carbonate in the prepared sodium hydrogen phosphate aqueous solution, respectively, adding ethanol, and then stirring at 150 r / min for 5 min to obtain a pretreatment liquid;

[0041] (2) subjecting the pretreated liquid to low-temperature coagulation treatment, and then thawing it at 10°C, and then dialyzing it, with a dialysis molecular weight cutoff of 3500Da, for 24 hours, and then diluting it 10 times, and then subjecting it to wet heat crystallization treatment, with a treatment temperature of 100°C, for 20 minutes, to obtain a highly crystalline silk fibroin microsphere liquid, and spray drying the highly crystalline silk fibroin microsphere liquid to obtain solid silk fibroin micro-nanospheres;

[0042] (3) After repeatedly washing the solid silk fibroin micro-nanospheres with deionized water by centrifugation, the micro-nanospheres were transferred to 30 mL of a 10 mg / mL sodium hydrogen phosphate aqueous solution, and then placed in a high-pressure reactor for reaction at 100° C. for 2 h. After naturally cooling to room temperature, the micro-nanospheres were washed with ethanol and deionized water in turn, and then freeze-dried to obtain silk fibroin micro-nanospheres;

[0043] (4) Sodium chloride aqueous solutions containing 3 mg / mL sodium polystyrene sulfonate, chitosan, and sodium hyaluronate were prepared respectively, and the pH value of the solutions was adjusted to 6.5. Then, the silk fibroin micro-nanospheres were immersed in the sodium chloride aqueous solution of sodium polystyrene sulfonate, the sodium chloride aqueous solution of chitosan, and the sodium chloride aqueous solution of sodium hyaluronate in turn for 20 min. After each immersion, the product was repeatedly centrifuged and washed with a 0.5 mol / L sodium chloride aqueous solution. Then, the product was etched 5 times with a 0.1 mol / L acetic acid aqueous solution, each etching time for 2 h, and then centrifuged and washed with deionized water, and then freeze-dried after low-temperature coagulation treatment to obtain composite silk fibroin micro-nanospheres.

[0044] (5) uniformly mixing monoglyceride, glyceryl stearate, olive oil and horse oil to obtain an oil phase; uniformly mixing water, madecassoside, sodium heparin, paeonol and bisabolol to obtain an emulsified composition; mixing the oil phase and the aqueous phase of the emulsified composition, stirring for 30 minutes, and then mechanically shearing for 3 minutes at a speed of 1000 rad / min to obtain an oil-in-water micron dispersion; finally, mechanically treating the oil-in-water micron dispersion at 1000 bar to obtain emulsified particles containing repair components;

[0045] (6) The composite silk fibroin micro-nanospheres are placed in a vacuum impregnation tank, evacuated to 20 Pa, maintained for 10 minutes, and then the emulsified particles are injected, wherein the ratio of the composite silk fibroin micro-nanospheres to the emulsified particles is 1 g:6 mL. The tank is maintained for 1 hour, and then the pressure is slowly released to normal pressure, and the vacuum is again evacuated to 1 Pa and maintained for 30 minutes. After the pressure is released to normal pressure, the product is taken out, the water is drained, and it is naturally dried to obtain the repair nanocarrier.

[0046] The preparation method of a moisturizing facial cream with redness-removing effect comprises the following preparation steps:

[0047] S1': weigh 40 parts of Artemisia annua volatile oil, 10 parts of camellia oil, 5 parts of repair nanocarriers, 5 parts of squalane, 1 part of cetearyl alcohol, and 1 part of ceramide respectively by weight;

[0048] S2': weigh 50 parts of deionized water, 15 parts of glycerol, 5 parts of sodium polyglutamate, 5 parts of trehalose, and 5 parts of lecithin respectively by weight to obtain an aqueous phase;

[0049] S3': The oil phase is added dropwise to the water phase at a mass ratio of 1:5 at a rate of 5 mL / min, the mixture is stirred and homogenized to obtain a homogenous emulsion, which is allowed to stand for degassing and then sterilized by ultraviolet light to prepare a moisturizing facial cream with redness-removing effect.

[0050] Example 2

[0051] A moisturizing facial cream with redness repairing effect comprises the following substances in parts by weight: an oil phase: 50 parts of artemisia annua volatile oil, 20 parts of camellia oil, 10 parts of repairing nano-carriers, 10 parts of squalane, 5 parts of cetearyl alcohol, and 2 parts of ceramide; an aqueous phase: 60 parts of deionized water, 20 parts of glycerol, 10 parts of sodium polyglutamate, 10 parts of trehalose, and 10 parts of lecithin.

[0052] The repair nano-carrier is prepared by loading emulsified particles containing repair ingredients with composite silk fibroin micro-nanospheres as a matrix and a carrier, wherein the emulsified particles are oil-in-water multi-structure emulsified particles; the multi-structure emulsified particles include an outer oil phase and an inner emulsified composition, and the outer oil phase includes 20 parts of monoglyceride, 10 parts of glyceryl stearate, 10 parts of olive oil, and 10 parts of horse oil in parts by weight; the inner emulsified composition containing the repair ingredient includes 25 parts of water, 20 parts of madecassoside, 10 parts of sodium heparin, 10 parts of paeonol, and 5 parts of bisabolol.

[0053] The preparation method of the repair nanocarrier is as follows:

[0054] (1) taking 60 mL of a 20 mg / mL sodium hydrogen phosphate aqueous solution, adjusting the pH to 6.5, dissolving 1.5 g of silk fibroin, 0.5 g of calcium nitrate and 1.0 g of sodium carbonate in the prepared sodium hydrogen phosphate aqueous solution, adding ethanol, and then stirring at 200 r / min for 5 min to obtain a pretreatment liquid;

[0055] (2) subjecting the pretreated liquid to low-temperature coagulation treatment, and then thawing it at 30° C., and then dialyzing it, with a dialysis molecular weight cutoff of 3500 Da, for 48 hours, and then diluting it 20 times, and then subjecting it to wet heat crystallization treatment, with a treatment temperature of 115° C. for 20 minutes, to obtain a highly crystalline silk fibroin microsphere liquid, and spray drying the highly crystalline silk fibroin microsphere liquid to obtain solid silk fibroin micro-nanospheres;

[0056] (3) After repeatedly washing the solid silk fibroin micro-nanospheres with deionized water by centrifugation, the micro-nanospheres were transferred to 50 mL of a 20 mg / mL sodium hydrogen phosphate aqueous solution, and then placed in a high-pressure reactor for reaction at 120° C. for 4 h. After naturally cooling to room temperature, the micro-nanospheres were washed with ethanol and deionized water in turn, and then freeze-dried to obtain silk fibroin micro-nanospheres;

[0057] (4) Sodium chloride aqueous solutions containing 5 mg / mL sodium polystyrene sulfonate, chitosan, and sodium hyaluronate were prepared respectively, and the pH value of the solutions was adjusted to 6.5. Then, the silk fibroin micro-nanospheres were immersed in the sodium chloride aqueous solution of sodium polystyrene sulfonate, the sodium chloride aqueous solution of chitosan, and the sodium chloride aqueous solution of sodium hyaluronate in turn for 20 min. After each immersion, the product was repeatedly centrifuged and washed with a 0.8 mol / L sodium chloride aqueous solution. Then, the product was etched with a 0.3 mol / L acetic acid aqueous solution for 5 times, each etching for 2 h, and then centrifuged and washed with deionized water, and then freeze-dried after low-temperature coagulation treatment to obtain composite silk fibroin micro-nanospheres.

[0058] (5) Monoglyceride, glyceryl stearate, olive oil, and horse oil are mixed uniformly to obtain an oil phase; water, madecassoside, sodium heparin, paeonol, and bisabolol are mixed uniformly to obtain an emulsified composition; the oil phase and the aqueous phase of the emulsified composition are mixed and stirred for 50 minutes, and then mechanically sheared at a speed of 1000 rad / min for 3 minutes to obtain an oil-in-water micron dispersion; finally, the oil-in-water micron dispersion is treated by a mechanical method at 1000 bar to obtain emulsified particles containing repair components;

[0059] (6) Place the composite silk fibroin micro-nanospheres in a vacuum impregnation tank, evacuate to 50Pa, maintain for 30 minutes, inject emulsified particles, wherein the ratio of composite silk fibroin micro-nanospheres to emulsified particles is 1g:3mL, continue to maintain for 2 hours, then slowly release the pressure to normal pressure, evacuate again to 5Pa, and maintain for 50 minutes. After the pressure is released to normal pressure, take out the product, drain the water and dry it naturally to obtain the repair nanocarrier.

[0060] The preparation method of a moisturizing facial cream with redness-removing effect comprises the following preparation steps:

[0061] S1': weigh 50 parts of Artemisia annua volatile oil, 20 parts of camellia oil, 10 parts of repair nanocarriers, 10 parts of squalane, 5 parts of cetearyl alcohol, and 2 parts of ceramide respectively by weight;

[0062] S2': weigh 60 parts of deionized water, 20 parts of glycerol, 10 parts of sodium polyglutamate, 10 parts of trehalose, and 10 parts of lecithin respectively by weight to obtain an aqueous phase;

[0063] S3': The oil phase is added dropwise to the water phase at a mass ratio of 1:5 at a rate of 5 mL / min, the mixture is stirred and homogenized to obtain a homogenous emulsion, which is allowed to stand for degassing and then sterilized by ultraviolet light to prepare a moisturizing facial cream with redness-removing effect.

[0064] Example 3

[0065] A moisturizing facial cream with redness repairing effect comprises the following substances in parts by weight: an oil phase: 45 parts of artemisia annua volatile oil, 15 parts of camellia oil, 8 parts of repairing nano-carriers, 8 parts of squalane, 3 parts of cetearyl alcohol, and 2 parts of ceramide; an aqueous phase: 55 parts of deionized water, 18 parts of glycerol, 8 parts of sodium polyglutamate, 8 parts of trehalose, and 8 parts of lecithin.

[0066] The repair nano-carrier is prepared by loading emulsified particles containing repair ingredients with composite silk fibroin micro-nanospheres as a matrix and a carrier, wherein the emulsified particles are oil-in-water multi-structure emulsified particles; the multi-structure emulsified particles include an outer oil phase and an inner emulsified composition, and the outer oil phase includes 15 parts of monoglyceride, 8 parts of glyceryl stearate, 8 parts of olive oil, and 8 parts of horse oil in parts by weight; the inner emulsified composition containing the repair ingredient includes 23 parts of water, 15 parts of madecassoside, 8 parts of sodium heparin, 8 parts of paeonol, and 3 parts of bisabolol.

[0067] The preparation method of the repair nanocarrier is as follows:

[0068] (1) taking 50 mL of a 15 mg / mL sodium hydrogen phosphate aqueous solution, adjusting the pH to 6.5, dissolving 1.3 g of silk fibroin, 0.4 g of calcium nitrate and 0.8 g of sodium carbonate in the prepared sodium hydrogen phosphate aqueous solution, adding ethanol, and then stirring at 150 r / min for 4 min to obtain a pretreatment liquid;

[0069] (2) subjecting the pretreated liquid to low-temperature coagulation treatment, and then thawing it at 20° C., and then dialyzing it, with a dialysis molecular weight cutoff of 3500 Da, for 36 hours, and then diluting it 15 times, and then subjecting it to wet heat crystallization treatment, with a treatment temperature of 120° C. for 20 minutes, to obtain a highly crystalline silk fibroin microsphere liquid, and spray drying the highly crystalline silk fibroin microsphere liquid to obtain solid silk fibroin micro-nanospheres;

[0070] (3) After repeatedly washing the solid silk fibroin micro-nanospheres with deionized water by centrifugation, the solid silk fibroin micro-nanospheres were transferred to 40 mL of a 15 mg / mL sodium hydrogen phosphate aqueous solution, and then placed in a high pressure reactor for reaction at 110° C. for 3 h. After naturally cooling to room temperature, the solid silk fibroin micro-nanospheres were washed with ethanol and deionized water in turn, and then freeze-dried to obtain silk fibroin micro-nanospheres;

[0071] (4) Sodium chloride aqueous solutions containing 3 mg / mL sodium polystyrene sulfonate, chitosan, and sodium hyaluronate were prepared respectively, and the pH value of the solutions was adjusted to 6.5. Then, the silk fibroin micro-nanospheres were immersed in the sodium chloride aqueous solution of sodium polystyrene sulfonate, the sodium chloride aqueous solution of chitosan, and the sodium chloride aqueous solution of sodium hyaluronate in turn for 20 min. After each immersion, the product was repeatedly centrifuged and washed with a 0.6 mol / L sodium chloride aqueous solution. Then, the product was etched 5 times with a 0.2 mol / L acetic acid aqueous solution, each etching time for 3 h, and then centrifuged and washed with deionized water, and then freeze-dried after low-temperature coagulation treatment to obtain composite silk fibroin micro-nanospheres.

[0072] (5) Monoglyceride, glyceryl stearate, olive oil, and horse oil are mixed uniformly to obtain an oil phase; water, madecassoside, sodium heparin, paeonol, and bisabolol are mixed uniformly to obtain an emulsified composition; the oil phase and the aqueous phase of the emulsified composition are mixed and stirred for 40 minutes, and then mechanically sheared at a speed of 1000 rad / min for 3 minutes to obtain an oil-in-water micron dispersion; finally, the oil-in-water micron dispersion is treated by a mechanical method at 1000 bar to obtain emulsified particles containing repair components;

[0073] (6) The composite silk fibroin micro-nanospheres were placed in a vacuum impregnation tank, evacuated to 30 Pa, maintained for 20 min, and then the emulsified particles were injected, wherein the ratio of the composite silk fibroin micro-nanospheres to the emulsified particles was 1 g:5 mL. The tank was maintained for 1.5 h, and then the pressure was slowly released to normal pressure, and the tank was evacuated again to 2.5 Pa and maintained for 40 min. After the pressure was released to normal pressure, the product was taken out, the water was drained, and it was naturally dried to obtain the repair nanocarrier.

[0074] The preparation method of a moisturizing facial cream with redness-removing effect comprises the following preparation steps:

[0075] S1': weigh 45 parts of Artemisia annua volatile oil, 15 parts of camellia oil, 8 parts of repair nanocarriers, 8 parts of squalane, 3 parts of cetearyl alcohol, and 2 parts of ceramide respectively by weight;

[0076] S2': weigh 55 parts of deionized water, 18 parts of glycerol, 8 parts of sodium polyglutamate, 8 parts of trehalose, and 8 parts of lecithin respectively by weight to obtain an aqueous phase;

[0077] S3': The oil phase is added dropwise to the water phase at a mass ratio of 1:5 at a rate of 5 mL / min, the mixture is stirred and homogenized to obtain a homogenous emulsion, which is allowed to stand for degassing and then sterilized by ultraviolet light to prepare a moisturizing facial cream with redness-removing effect.

[0078] Comparative Example 1: This comparative example 1 is basically the same as Example 3, except that it does not contain repair nanocarriers.

[0079] Comparative Example 2: This comparative example 2 is basically the same as Example 3, except that step (4) is omitted in the preparation of the repair nanocarrier.

[0080] Comparative Example 3: This comparative example is basically the same as Example 3, except that emulsified particles containing repair components are used instead of repair nanocarriers.

[0081] Test experiment:

[0082] 1. Moisturizing efficacy test

[0083] Six samples of the creams of Examples 1-3 and Comparative Examples 1-3 were used as test samples. 60 volunteers (aged between 24 and 40 years old) who met the test conditions were screened and randomly divided into 6 groups, 10 people in each group, and one sample was tested in each group. The test area and a blank area of ​​equal size were marked on the inside of the volunteer's arm, and the test sample was applied in the test area at a dosage of 2.0 mg ± 0.1 mg of the test sample / cm2, twice a day, once in the morning and once in the evening, for 28 consecutive days; the blank area was not coated with a sample to form a blank group. On the 0th day and the 28th day, the compact MoistureMeter SC instrument of Delfin, Finland, was used to measure the skin moisture content in the stratum corneum of the test area, and the VapoMeter moisture evaporation tester of Delfin, Finland, was used to test the transepidermal water loss of the test area, and the average values ​​were taken respectively.

[0084] Skin moisture content change rate = (skin moisture content after using the sample - initial skin moisture content) / initial skin moisture content × 100%;

[0085] Skin transepidermal water loss change rate = (transepidermal water loss after using the sample - initial transepidermal water loss) / initial transepidermal water loss × 100%.

[0086] Table 1 Sample moisturizing effect test results

[0087] sample Skin moisture content change rate Transepidermal water loss rate Example 1 45.5％ -53.7％ Example 2 49.2％ -56.1％ Example 3 44.6％ -48.9％ Comparative Example 1 8.7％ -11.8％ Comparative Example 2 32.1％ -25.5％ Comparative Example 3 15.0％ -16.8％ blank 3.5％ -2.8％

[0088] As can be seen from Table 1, the moisturizing cream of the present invention can effectively repair the skin, shorten the repair time, and accelerate the healing of the skin. The test results show that in the area where the red repair essence lotion prepared by Examples 1, 2 and 3 was used, the skin water content increased significantly, and the skin water content change rate after 28 days of use was greater than 44% (i.e., the skin water content increased by 44%), which was significantly higher than the control group and the blank group. The red repair essence prepared by Examples 1, 2 and 3 had a skin transepidermal water loss change rate of greater than 48% after 28 days of use, which was significantly higher than the control group and the blank group. The results show that the repair nano-carrier obtained by using silk fibroin micro-nanospheres as a carrier and infiltrating emulsified particles containing repair ingredients into the carrier can keep the repair ingredients on the skin for a long time, increase the skin water content, effectively reduce water loss, and improve the skin barrier function.

[0089] 2. Human trials

[0090] Testers and methods: 60 volunteers, including volunteers with fragile skin who are prone to redness due to allergies, those who are currently experiencing redness due to allergies, and those with obvious red blood streaks on their faces, aged 28-50 years old; the volunteers were randomly divided into 6 groups, each with 10 people, and each group was tested on half of the face. Each group tested one sample, and after 28 days of continuous use, the volunteers were surveyed and subjected to VISIA image analysis (photographed and analyzed before and after use), and blood flow (tested before and after use).

[0091] 2.1 Questionnaire Survey Results

[0092] During the test period, 60 volunteers did not experience any allergic reactions or other discomfort when using the test samples. Among them, 43 people felt that using one side of the sample of Experimental Example 3 was effective, as manifested in that red blood streaks were reduced, inflammation was relieved, and allergic reactions did not occur on skin that is prone to allergies.

[0093] 2.2VISIA Image Analysis Red Zone

[0094] Using the before-and-after VISIA image analysis, a macroscopic comparative analysis of the red area was performed, and the improvement of the red area was divided into three levels: Grade I ineffective (no obvious difference before and after), Grade II slightly improved (1-2 red areas improved), and Grade III significantly improved (more than 2 red areas improved). The analysis results are shown in Table 2.

[0095] Table 2 Human trial results

[0096] sample Ⅰ Ⅱ Ⅲ Example 1 (Number of testers: 60) 3 11 46 Example 2 (Number of testers: 60) 3 14 43 Example 3 (Number of testers: 60) 2 10 48 Comparative Example 1 (Number of test subjects: 60) 12 18 30 Comparative Example 2 (Number of testers: 60) 35 14 11 Comparative Example 3 (Number of test subjects: 60) 47 5 8

[0097] The results show that the samples of Examples 1-3 can improve the condition of the red area, and it can be seen that the exposed capillaries and inflammation are improved, and the redness phenomenon is reduced. The cream in Experimental Example 3 has a more significant effect, and the embodiment group is significantly better than the comparative group.

[0098] 2.3 Blood flow

[0099] Instrument: DOPPLER-POWER Doppler energy ultrasound

[0100] Blood flow rate = (blood flow after use - blood flow before use) / blood flow after use * 100%

[0101] Example 1: Average blood flow rate change rate is -48.60%

[0102] Example 2: Average blood flow rate change rate is -49.08%

[0103] Example 3: Average blood flow rate change rate is -49.57%

[0104] Comparative Example 1: Average blood flow rate change rate is -6.35%

[0105] Comparative Example 2: Average blood flow rate change rate is -21.50%

[0106] Comparative Example 3: Average blood flow rate change rate is -25.97%

[0107] By comparison, it can be seen that after using Examples 1-3, the blood flow rate is significantly reduced, indicating that it has the effect of improving microcirculation and can enhance the elasticity of blood vessel walls.

[0108] The present invention is not limited to the above optional implementations, and anyone can derive other various forms of products under the enlightenment of the present invention. The above specific implementations should not be understood as limiting the scope of protection of the present invention. The scope of protection of the present invention should be based on the definition in the claims, and the description can be used to interpret the claims.

Claims

1. A moisturizing facial cream with redness-removing effect, characterized in that: In terms of weight, the composition includes the following substances: oil phase: 40-50 parts of Artemisia annua volatile oil, 10-20 parts of camellia oil, 5-10 parts of repair nanocarriers, 5-10 parts of squalane, 1-5 parts of cetearyl alcohol, and 1-2 parts of ceramide; water phase: 50-60 parts of deionized water, 15-20 parts of glycerol, 5-10 parts of sodium polyglutamate, 5-10 parts of trehalose, and 5-10 parts of lecithin.

2. A moisturizing facial cream with redness-reducing effect according to claim 1, characterized in that: The repair nano-carrier is prepared by vacuum impregnation loading emulsified particles containing repair components using composite silk fibroin micro-nanospheres as a matrix and composite silk fibroin micro-nanospheres as a carrier, wherein the emulsified particles are oil-in-water multi-structure emulsified particles; the multi-structure emulsified particles include an outer oil phase and an inner emulsified composition, wherein the outer oil phase includes 10-20 parts of monoglyceride, 5-10 parts of glyceryl stearate, 5-10 parts of olive oil, and 5-10 parts of horse oil in parts by weight; the inner emulsified composition containing the repair component includes 20-25 parts of water, 10-20 parts of madecassoside, 5-10 parts of sodium heparin, 5-10 parts of paeonol, and 1-5 parts of bisabolol; the inner emulsified composition is wrapped by the outer oil phase.

3. A moisturizing facial cream with redness-reducing effect according to claim 2, characterized in that: The preparation method of the composite silk fibroin micro-nanosphere is as follows: (1) taking 40-60 mL of a disodium hydrogen phosphate aqueous solution, adjusting the pH to 6.0-6.8, dissolving 1.0-1.5 g of silk fibroin, 0.3-0.5 g of calcium nitrate and 0.5-1.0 g of sodium carbonate in the prepared disodium hydrogen phosphate aqueous solution, adding ethanol, and then stirring at 100-200 r / min for 3-5 min to obtain a pretreatment liquid; (2) subjecting the pretreated liquid to low-temperature coagulation treatment, and then thawing, dialyzing, and diluting treatment, and then subjecting the pretreated liquid to wet heat crystallization treatment at a treatment temperature of 100 to 130° C. for 15 to 30 minutes to obtain a highly crystalline silk fibroin microsphere liquid, and spray drying the highly crystalline silk fibroin microsphere liquid to obtain solid silk fibroin micro-nanospheres; (3) After repeatedly washing the solid silk fibroin micro-nanospheres with deionized water by centrifugation, the micro-nanospheres were transferred to 30-50 mL of a sodium dihydrogen phosphate aqueous solution, and then placed in a high-pressure reactor to react at 100-120° C. for 2-4 h. After naturally cooling to room temperature, the micro-nanospheres were washed with ethanol and deionized water in turn, and then freeze-dried to obtain silk fibroin micro-nanospheres; (4) Sodium chloride aqueous solutions containing 1-5 mg / mL sodium polystyrene sulfonate, chitosan, and sodium hyaluronate were prepared respectively, and the pH values ​​of the solutions were adjusted to 6.0-6.

8. Then, the silk fibroin micro-nanospheres were immersed in the sodium chloride aqueous solutions of sodium polystyrene sulfonate, chitosan, and sodium chloride aqueous solutions of sodium hyaluronate in turn for 15-25 min. After each immersion, the product was repeatedly centrifuged and washed with the sodium chloride aqueous solution. Then, the product was etched with an acetic acid aqueous solution for 3-5 times, each etching time for 2-3 h, and then centrifuged and washed with deionized water. After low-temperature coagulation treatment, freeze-drying was performed to obtain composite silk fibroin micro-nanospheres.

4. The moisturizing facial cream with redness-reducing effect according to claim 3, characterized in that: The concentration of the sodium hydrogen phosphate aqueous solution in step (1) and step (3) is 10 to 20 mg / mL.

5. The moisturizing facial cream with redness-reducing effect according to claim 3, characterized in that: In the step (2), the thawing temperature is 10-30° C., the dilution multiple is 1-20 times; the molecular weight cutoff of the dialysis material for dialysis is 3500 Da, and the dialysis time is 24-48 hours, wherein the dialysis water is replaced every 1-2 hours.

6. The moisturizing facial cream with redness-reducing effect according to claim 3, characterized in that: The low-temperature coagulation treatment in steps (2) and (4) is freezing at a temperature of -80°C to -20°C for 8 to 24 hours; the concentration of the sodium chloride aqueous solution in step (4) is 0.5 to 0.8 mol / L, and the concentration of the acetic acid aqueous solution is 0.1 to 0.3 mol / L.

7. The moisturizing facial cream with redness-reducing effect according to claim 1, characterized in that: The preparation method of the repair nanocarrier is as follows: S1: uniformly mix monoglyceride, glyceryl stearate, olive oil and horse oil to obtain an oil phase; uniformly mix water, madecassoside, sodium heparin, paeonol and bisabolol to obtain an emulsified composition; after mixing the oil phase and the aqueous phase of the emulsified composition, continue stirring for 30 to 50 minutes, and then mechanically shear for 1 to 3 minutes at a speed of 200 to 2000 rad / min to obtain an oil-in-water micron dispersion; finally, mechanically treat the oil-in-water micron dispersion at 200 to 2000 bar to obtain emulsified particles containing repair components; S2: Place the composite silk fibroin micro-nanospheres in a vacuum impregnation tank, evacuate to 10-50Pa, maintain for 10-30 minutes, inject emulsified particles, continue to maintain for 1-2 hours, then slowly release the pressure to normal pressure, evacuate again to 1-5Pa, and maintain for 30-50 minutes. After the pressure is released to normal pressure, take out the product, drain the water and dry it naturally to obtain the repair nano-carrier.

8. The moisturizing facial cream with redness-reducing effect according to claim 7, characterized in that: The usage ratio of the composite silk fibroin micro-nanospheres to the emulsified particles is (5-10) g: (20-30) mL.

9. A method for preparing a moisturizing facial cream with redness-reducing effect according to any one of claims 1 to 8, characterized in that: The method comprises the following steps: S1': weigh 40-50 parts of Artemisia annua volatile oil, 10-20 parts of camellia oil, 5-10 parts of repair nanocarriers, 5-10 parts of squalane, 1-5 parts of cetearyl alcohol, and 1-2 parts of ceramide respectively by weight; S2': weigh 50-60 parts of deionized water, 15-20 parts of glycerol, 5-10 parts of sodium polyglutamate, 5-10 parts of trehalose, and 5-10 parts of lecithin respectively by weight to obtain an aqueous phase; S3': Slowly add the oil phase to the water phase at a mass ratio of 1:5, stir and mix, and homogenize to obtain a homogenous emulsion. After standing to degas, sterilize with ultraviolet light to prepare a moisturizing cream with redness-removing effect.

10. The method for preparing a moisturizing facial cream with redness-reducing effect according to claim 9, characterized in that: The slow rate in step S3' is 5 mL / min.