Application of probiotics combined with Phyllanthus emblica extract to improvement of diabetes and metabolic syndrome

A technology of metabolic syndrome and probiotics, applied in the field of medicine, can solve the problems of unreported diabetes and metabolic syndrome

Inactive Publication Date: 2019-12-24
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Amla and probiotics have complementary medicinal effects, but so far, no report has found that the joint use of the two can promote each other to improve diabetes and metabolic syndrome and alleviate its complications

Method used

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  • Application of probiotics combined with Phyllanthus emblica extract to improvement of diabetes and metabolic syndrome
  • Application of probiotics combined with Phyllanthus emblica extract to improvement of diabetes and metabolic syndrome
  • Application of probiotics combined with Phyllanthus emblica extract to improvement of diabetes and metabolic syndrome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 In this experiment, Bifidobacteria and Streptococcus thermophilus were taken as examples. Amla emblica and its extracts promote the growth of probiotics and inhibit the growth of harmful bacteria

[0023] Resuscitate Bifidobacterium, Streptococcus thermophilus, Escherichia coli and Staphylococcus aureus, use lactic acid bacteria medium for Bifidobacterium, M17 medium for Streptococcus thermophilus, LB medium for Escherichia coli, and beef extract peptone for Staphylococcus aureus culture medium. After overnight recovery at 37°C, they were added to the culture medium at a ratio of 1:1000, and different concentrations (0, 0.01, 0.05, 0.1, 0.5, 1 mg / mL) of Amla extract were given, and 6 parallel experiments were set up for each concentration. Wells, after growing at 37°C for 8h, adopt OD 600 Detect the growth of Bifidobacterium, Streptococcus thermophilus, Escherichia coli and Staphylococcus aureus.

[0024] Table 1 The effect of emblica extract on the growth o...

Embodiment 2

[0029] Example 2 Phyllanthus emblica and its extract combined with probiotics to control weight gain

[0030] Select 5-week-old male C57 mice (Qinglongshan Animal Experiment Center, Nanjing, Jiangsu), weighing 20-24 g, male, and divide them into normal group, control group (fed with high-fat feed), and administration group 1 (Emblica emblica extract). medicine), administration group 2 (probiotics) and administration group 3 (combined action of emblica and probiotics), 10 rats in each group, half male and half male. The normal group was given a normal diet, and the other four groups were fed with high-fat feed. While feeding the high-fat feed, different drugs were given orally, and the body weight of mice in each group was recorded every 2 weeks for a total of 12 weeks. The body weight growth of the mice in the drug group.

[0031] Table 2 Effects of emblica extract combined with probiotics on weight gain in mice

[0032]

[0033] *Compared with the control group, P<0.05; ...

Embodiment 3

[0035] Example 3 Amla and its extracts combined with probiotics reduce blood sugar in diabetic mice induced by high-fat diet

[0036] Oral glucose tolerance test (OGTT) in high-fat diet-induced diabetic mice: 5-week-old C57 mice (Qinglongshan Animal Experiment Center, Nanjing, Jiangsu), weighing 20-24 g, male, were used to induce obesity in mice with high-fat diet . The modeled mice were randomly divided into 4 groups, respectively control group, probiotic group (100mg / kg), emblica group (100mg / kg), emblica extract combined probiotic group (100mg / kg+100mg / kg ), 8 rats in each group, administered orally every day for 12 consecutive weeks. At the end of the experiment, the mice were fasted without food and water for 12 hours before the experiment, administered glucose (2 g / kg) by gavage, and blood was collected from the tail to measure the blood sugar level at 0, 15, 30, 60, and 120 minutes after the administration.

[0037] Table 3 Effects of emblica and its extracts combined...

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Abstract

The invention relates to the technical field of biology, in particular to application of probiotics, which is combined with a phyllanthus emblica xtract in regulating blood sugar and blood fat, improving diabetes and metabolic syndrome, slowing down occurrence of related complications and further maintaining metabolic stability of organisms. The probiotics is an active microorganism beneficial toa host, can regulate intestinal flora balance, and can generate an exact health effect so as to improve the micro-ecological balance of the host and play a beneficial role; the phyllanthus emblica hasthe effects of regulating blood sugar, enhancing body immunity and the like. The phyllanthus emblica is found to play a role in reducing blood sugar and blood fat; the phyllanthus emblica component can provide a good environment for the growth of probiotics; meanwhile, the probiotics are promoted to regulate glucose uptake, and the gastrointestinal tract stimulation effect generated by taking theprobiotics alone is improved, so that the probiotics and the phyllanthus emblica can better play a role in reducing blood sugar, complications are reduced, and the material can be prepared into a pharmaceutical composition for preventing or treating diabetes and metabolic syndrome. Therefore, the invention provides a new application for improving diabetes and metabolic syndrome related diseases.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a new application of probiotics combined with emblica and its extracts for improving complications related to diabetes and metabolic syndrome. Background technique [0002] Diabetes is a disease of energy metabolism, which is mainly divided into type 1 diabetes (insulin-dependent diabetes) and type 2 diabetes (non-insulin-dependent diabetes). At present, there are about 366 million diabetic patients in the world, accounting for 6.4% of the world's population, and type 2 diabetic patients account for about 90-95% of the total number of diabetic patients. The pathogenesis of type 2 diabetes is very complex. In addition to changes in the human genome, eating habits or reduced physical activity leading to diabetes, changes in the structure of intestinal flora are related to the occurrence and development of diabetes. As an environmental factor, intestinal flora can sign...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/745A61K35/744A61K36/47A61K45/06A61P3/10A61P3/00
CPCA61K35/745A61K35/744A61K36/47A61K45/06A61P3/10A61P3/00A61K2300/00
Inventor 张瑜于倩倩徐郁蕊刘晓平宁兴海
Owner NANJING UNIV
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