Method for isolating and detecting oxidized triglyceride (ox-TG) of edible vegetable oil and application of the method

A technology for edible vegetable oil and oxidized glycerol, which is applied in the field of separation and detection of oxidized glycerides in edible vegetable oil, and can solve the problems of difficult effective and accurate detection of edible vegetable oil, difficult separation and accurate detection, etc.

Active Publication Date: 2012-07-04
SHANGHAI LIANGYOU GRP +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0032] The purpose of the present invention is to provide a method for separating and detecting oxidized glycerides in edible vegetable oils, so as to solve the problem that it is difficult to separate and accurately detect the content of oxidized glycerides in edible vegetable oils in the prior art. An important step to accurately detect whether the edible vegetable oil is mixed with kitchen waste oil

Method used

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  • Method for isolating and detecting oxidized triglyceride (ox-TG) of edible vegetable oil and application of the method
  • Method for isolating and detecting oxidized triglyceride (ox-TG) of edible vegetable oil and application of the method
  • Method for isolating and detecting oxidized triglyceride (ox-TG) of edible vegetable oil and application of the method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Comparison of oxidized glyceride content in different samples.

[0080] Detection method of oxidized glyceride content:

[0081] One), the extraction of oil polar components

[0082] Method 1) Ordinary column chromatography: first accurately weigh 2.5 g (M) of the oil sample, dissolve it completely with 20 mL of petroleum ether with a boiling range of 30 to 60 °C, and then dilute to volume with petroleum ether with a boiling range of 30 to 60 °C. 50mL, take 20mL of the sample solution and put it on a silica gel chromatography column (a glass column with an inner diameter of 21mm and a length of 450mm, filled with 25 grams of 100-200 mesh silica gel with a water content of 5%), and wash with 150mL of washing solution (30-60℃ Petroleum ether in the boiling range: diethyl ether = 87:13) wash the silica gel chromatography column at a flow rate of 2 to 2.5 mL / min, discard the washing solution; then use 150 mL of diethyl ether to elute the silica gel layer at a flow rate of ...

Embodiment 2

[0122] sample testing

[0123] Sample source: frying pan from a restaurant

[0124] Detection method:

[0125] Step 1: Quantitative detection of oxidized glycerides in the sample

[0126] One), the extraction of oil polar components

[0127] Method 1) Ordinary column chromatography: first accurately weigh 2.5 g (M) of the oil sample, dissolve it completely with 20 mL of petroleum ether with a boiling range of 30 to 60 °C, and then dilute to volume with petroleum ether with a boiling range of 30 to 60 °C. 50mL, take 20mL of the sample solution and put it on a silica gel chromatography column (a glass column with an inner diameter of 21mm and a length of 450mm, filled with 25g of 100-500 mesh silica gel with a water content of 5%), and wash with 150mL of washing solution (30-60℃ Petroleum ether in the boiling range: diethyl ether = 87:13) wash the silica gel chromatography column at a flow rate of 2 to 2.5 mL / min, discard the washing solution; then use 150 mL of diethyl ether...

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Abstract

The invention belongs to the field of compound isolation and detection, and relates to a method for isolating and detecting oxidized triglyceride (ox-TG) of edible vegetable oil and application of the method. The method of the invention adopts a normal phase column chromatography method and HPLC-GPC technique to isolate oxidized triglyceride from edible vegetable oil, and combines with evaporative light scattering detector (ELSD) or differential refraction detector (RID) to analyze and detect the oxidized triglyceride content in the edible vegetable oil by the peak area of oxidized triglyceride in a chromatogram, and the above steps are all important steps for detecting whether the edible vegetable oil contains kitchen waste oil effectively and accurately. The method of the invention can directly isolate and detect oxidized triglyceride in the edible vegetable oil, and can be directly applied to qualitatively detecting whether the edible vegetable oil contains kitchen waste oil.

Description

technical field [0001] The invention belongs to the field of compound separation and detection, and relates to a method for separating and detecting oxidized glyceride in edible vegetable oil and its application. Background technique [0002] Kitchen waste oil, that is, gutter oil in a broad sense, mainly includes three categories: gutter oil (gutter oil in a narrow sense), swill oil (swill oil), and old frying oil. Drain oil refers to the greasy floating matter caught in the gutter grease traps of hotels, restaurants, etc., which is in the form of dark brown liquid paste and has a sour and foul smell. Swill oil refers to the upper layer of slick oil after leftovers and leftovers (commonly known as swill) are collected, which is golden yellow to dark red after refining, and has a sour smell. Old frying oil refers to the oil that has been repeatedly used for frying food at high temperatures, has deteriorated, and can no longer be eaten. [0003] The sensory and physicochemi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/08G01N30/02B01D15/08
Inventor 曹文明薛斌李奇章孙禧华
Owner SHANGHAI LIANGYOU GRP
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