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Method for preparing grease and protein from oil seed kernels by aqueous enzymatic method

A technology of oilseed kernel and water enzymatic method, which is applied in the directions of oil/fat production, fat production, fermentation, etc., can solve the problems of low oil yield and large water consumption, achieve high oil yield, improve oil yield, The effect of low energy consumption

Inactive Publication Date: 2012-10-24
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, at present, there are problems such as low oil yield and high water consumption in the aqueous enzymatic oil production. If the oil is squeezed and then used in the aqueous enzymatic oil production, the oil yield can be increased, and the water and enzyme consumption can be reduced, which will have broad prospects. Application prospect

Method used

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  • Method for preparing grease and protein from oil seed kernels by aqueous enzymatic method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The rapeseed containing 0.23% impurities by weight after passing through a plane rotary sieve, a destoner, a magnetic separator, and an air-selection is fed into a rolling mill, and the granular oil is rolled into a thin billet with a thickness of 0.3 mm. The green sheet was steamed and fried in a vertical steaming and frying pan, the moisture content after steaming and frying was 5% (weight %), and the temperature of the embryo was 110°C. The cooked embryos (ie oilseed kernels) after steaming and frying are directly pressed into the ZY24 pre-pressing machine. After pressing, the residual oil rate of the rapeseed cake is 12.5% ​​by weight, and the pressed cake is crushed to 80 mesh by the pulverizer. The pulverized press cake was dried at 100° C. for 5 min to inactivate the native enzyme system, and the processed press cake was obtained. Add 10 times the mass of water to the treated press cake (the amount of water added is 10 times the mass of the treated press cake), a...

Embodiment 2

[0024] like figure 1 As shown, the rapeseed containing 0.26% of impurities by weight after the plane rotary sieve, de-stoner, magnetic separator, and air selection is dried, and the moisture content after drying is 8.5% by weight. The rapeseed is peeled and separated from the skin and kernel by the YTTP-75 type peeling and separation system to obtain the rapeseed kernel. The skin content in the rapeseed kernel is 1.53% by weight. Feed and press to obtain a low-temperature pressed cake with a residual oil rate of 15.2% by weight, and the pressed cake enters a pulverizer and is pulverized to 40 mesh. The pulverized press cake was dried at 100° C. for 10 min to inactivate the native enzyme system, and the processed press cake was obtained. Add 20 times the mass of water to the treated press cake (the amount of water added is 20 times the mass of the treated press cake), and add 1.2% of the compound enzyme of the pressed cake mass (the amount of compound enzyme added is the amoun...

Embodiment 3

[0026] Dry the tea seeds containing 0.20% by weight of impurities after passing through a plane rotary sieve, a stone remover, a magnetic separator, and air selection, and the moisture content after drying is 11.0% by weight. YTTK-75 camellia seed shelling machine is used for peeling and separation of skin and kernel to obtain tea seed kernels. SSYZ 12 / 12 low-temperature screw oil press is used to feed at room temperature, and the low-temperature pressing with a residual oil rate of 13.8% by weight is obtained by pressing. The cake is pressed into the pulverizer and pulverized to 100 mesh. The pulverized press cake was dried at 100° C. for 8 minutes to inactivate the native enzyme system, and the processed press cake was obtained. Add 5 times the mass of water to the processed press cake, add 2.0% of the mass of the pressed cake compound enzyme, the compound enzyme is composed of pectinase and alkaline protease in a mass ratio of 1:1, adjust the pH to 4.5, and the temperature ...

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Abstract

The invention relates to a method for preparing grease and protein from oil seed kernels by an aqueous enzymatic method and belongs to the technical field of the application of the biotechnology to the grain and oil food processing. The method for preparing grease and protein from the oil seed kernels by the aqueous enzymatic method is characterized by comprising the following steps of 1) oil preparation through squeezing; 2) crushing; and 3) enzymolysis and drying: water and complex enzyme are added into treated squeezed cakes, the pH is regulated to the pH value and the temperature applicable to carbohydrase, and the enzymolysis is carried out for 15 to 90 minutes; the pH is regulated to the pH value and the temperature applicable to the protease, and the enzymolysis is carried out for 15 to 90 minutes; the pH is regulated to 9.0 after the enzyme reaction, and the stirring alkali extraction is carried out for 10 to 60 minutes at room temperature; boiled oil and protein water solution are separated through centrifugation; and the boiled oil is subjected to vacuum drying at 100 to 120 DEG C, grease products are obtained, the protein water solution is subjected to spraying drying, and plant protein is obtained. The method has the characteristics of high oil yield, low water consumption and low energy consumption, and the additional value of oil processing products can be improved.

Description

technical field [0001] The invention relates to a method for extracting oil and protein from oilseed kernels by using water enzymatic technology, and belongs to the technical field of application of biotechnology in grain and oil food processing. Background technique [0002] The water enzymatic method was born in the 1970s, but limited by the high cost of enzymes, the development is not fast. According to the review by Rosenthal et al.: Lanzani first applied the water enzymatic method to rapeseed oil, Lanzani used protease and pectinase, and the oil yield was 78%. Fullbrook tried to use the compound enzyme produced by Aspergillus niger to hydrolyze rapeseed, the yield of oil and protein was increased, and the oil extraction effect was better by adding organic solvent. In 1990, Fereidoon applied water enzymatic extraction of canola oil and protein in a pilot plant. They also used a complex enzyme derived from Aspergillus niger, which effectively degrades rapeseed cell wall...

Claims

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Application Information

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IPC IPC(8): C11B1/00C11B1/04C11B1/06C12P21/06
Inventor 黄凤洪钮琰星万楚筠黄庆德李文林胡双喜杨湄刘昌盛郭萍梅郑畅周琦郑明明杨金娥许继取
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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