A comprehensive utilization process of a glutamic acid crystal mother liquid comprises the following steps: one, leading the glutamic acid crystal mother liquid into a membrane separation system, allowing solid particles, thalli, proteins, polysaccharides and a colloid in the mother liquid to be intercepted by a membrane, and making glutamic acid left in a membrane filtrate; and allowing the membrane filtrate to go to a next step for treatment; two, leading the membrane filtrate into an ion exchange system, adsorbing glutamic acid by an ion exchange resin, when the content of glutamic acid in an ion exchange tail liquid is not more than 0.2%, stopping column-loading, adding an eluting agent, eluting, allowing glutamic acid to go into the eluate, collecting the eluate, and recycling glutamic acid from the eluate. The membrane separation technology and the ion exchange technology are combined, recycling of glutamic acid from the crystal mother liquid is realized, at the same time, a protein feed is prepared from the thalli and the proteins intercepted by the membrane, an organic fertilizer is prepared from the ion exchange tail liquid through slurry-spraying granulation, the whole process allows useful substances in the crystal mother liquid to be totally recycled, comprehensive utilization of the resources is thoroughly achieved, waste is turned into treasure, and significant economic benefits are achieved.