100results about How to "Reduce the amount of enzymes used" patented technology

The invention discloses a combined preprocessing method of lignocellulose. The lignocellulose is arranged in a recycle reactor after being crushed, and dilute acid is injected; a recycle pump starts up, the circular reaction is acted under the temperature ranging from 50 DEG C to 200 DEG C, and the obtained hydrolysate is disacidified and then is used for ferment after the reaction is finished; the surplus lignocellulose in the recycle reactor is put in a globe mill for ball milling through adding lye after the surplus lignocellulose is washed, and the surplus lignocellulose is used in subsequent zymohydrolysis after ball milling and alkali removing. The invention effectively increases the hemicellulose percent conversion, the lignin removal rate and the cellulose zymohydrolysis percent conversion in the lignocellulose, reduces fermentation inhibition products, and reduces the enzyme usage when in zymohydrolysis, shortens the reaction time and increases the sugar consistence at the same time; in addition, the invention can set the water consumption during the preprocessing according to the needs of subsequent ferment consistence.

The invention belongs to the field of rice deep processing technologies and in particular relates to a method for co-producing the high-purity rice starch and rice protein. The method is characterized in that washing rice used as a raw material, soaking, grinding to obtain thick liquid, separating, adjusting the pH of liquid supernatant, performing enzymolysis, washing, performing settlement separation, and then drying to obtain the high-purity rice protein; or obtained precipitate is subjected to alkaline protease purification, pH adjustment, ethanol purification, washing, settlement separation, drying to obtain a high-purity rice starch product. The method can be used for co-producing the rice starch and rice protein, and can effectively improve the purity and increase the yield of the product. The product obtained by using the method can be used as the raw material for deep processing of food.

A method for producing glucose and cellobiose using cellulose complex enzyme is the technology using the cellulose complex enzyme as a catalyst and realizing the high saccharification rate conversion of cellulose by adding cellulose enzyme activator at the optimized stirring speed. Glucose concentration can reach 150g/L after using cellulose enzymatic hydrolysis, and the method is a cellulose saccharification production technology with economic feasibility. With the technology used, low cost, low usage of enzyme and high saccharification rate can be realized, and the productions of cellulose alcohol and biochemical products (lactic acid, succinic acid and the like) are satisfied. In the whole technology, no beta-glucosaccharase is added additionally, no sugar concentrating device is added, and the technology is simple, the equipment cost is low and the industrialized prospect is good.

The invention relates to a method for synchronously extracting soybean oil, soybean milk powder and soybean dietary fiber by an aqueous enzymatic method, which belongs to the technical field of plant grease processing. The method comprises the following steps: 1) dehulling soybeans and crushing the soybeans, extruding and puffing the material to obtain a puffed product; 2) adding water into an enzymatic hydrolysis reaction vessel, heating steam and adding the puffed product to obtain a mixed liquor, performing alkaline extraction on the mixed liquor, adding alkali protease for performing enzymatic hydrolysis, using a horizontal centrifuge after enzymatic hydrolysis is carried out for performing centrifugation to obtain an emulsified oil, a hydrolysate and residues; 3) using a disk centrifuge for performing centrifugation on the emulsified oil to obtain the soybean oil and a water phase, adding the water phase into the hydrolysate for concentration, and drying the material to obtain the soybean milk powder; and 4) adding water for mixing the water and the residues for homogenizing and drying a mixture to obtain the soybean dietary fiber. The method has the advantages of simple process, less enzyme usage amount, short reaction time, and low cost. The prepared high-quality soybean oil can obtain the high-nutrition partly-defatted soybean milk powder and high-oil high-protein soybean dietary fiber, and is suitable for industrial continuous production.

The invention relates to a method for synchronously extracting soybean oil and soybean milk powder by an aqueous enzymatic method, which belongs to the technical field of plant grease processing. The method comprises the following steps: 1) dehulling soybeans and performing ultramicro grinding on the soybeans, extruding the soybeans and puffing the ground material to obtain a puffed product; 2) adding water into an enzymatic hydrolysis reaction vessel, heating steam and adding the puffed product to obtain mixed liquor, adjusting pH value of the mixed liquor, adding alkali protease for performing enzymatic hydrolysis, using a horizontal centrifuge after enzymatic hydrolysis is carried out for performing centrifugation to obtain emulsified oil, a hydrolysate and residues; and 3) using a disk centrifuge for performing centrifugation on the emulsified oil to obtain the soybean oil and a water phase, adding the water phase into the hydrolysate for concentration, and drying the material to obtain the soybean milk powder. The method has the advantages of simple process, short reaction time, less enzyme usage amount and low cost, and the prepared soybean oil has no solvent residues and has high quality. The soybean milk powder can be prepared when the soybean oil is prepared, the soybean milk powder can keep partial fat, and has advantages of rich bean fragrance, abundant nutrition, and good brewing property, and is suitable for continuous industrial production.

The invention provides a nano enzyme with a nanometer cage core of a hollow gold-platinum alloy and a shell of a porous silicon dioxide, a preparation method and an application thereof, and belongs tothe field of nanometer materials. The preparation method comprises the following steps: etching a gold core platinum shell nanostructure through potassium hexachloroplatinate to obtain a nanocage ofhollow gold-platinum alloy; coating silicon dioxide on the surface of the nanocage through tetraethyl silicate; and obtaining the nano enzyme taking the nanometer cage, in a round rod shape, of the hollow gold-platinum alloy as a core, and a silicon dioxide shell as the periphery of the core. The length of the core is 80 to 100 nm, and the diameter is 20 to 25 nm. The shell is provided with a channel of 3 to 5 nm. The nano enzyme is negatively charged. The antigen can be bound by an attractive effect of positive and negative electric charges to form a nano enzyme label antigen complex, which is used for performing enzyme linked immunoassay. According to the nano enzyme with the nanometer cage core of the hollow gold-platinum alloy and the shell of the porous silicon dioxide, the preparation method and the application thereof, the cost of a reagent of the natural enzyme label antigen complex is reduced, the working environment of enzyme linked immunodetection is extended, the defects that the natural enzyme is easy to deactivate and denature, etc., are overcame, and the nano enzyme can be widely applied to various enzyme linked immunodetection.

The invention relates to a biological preparation method of 6-cyano-(3R, 5R)-dihydroxyhexanoate. According to the biological preparation method, 6-cyano-(6R)-hydroxyl-3-oxo-tertiary butyl hydroperoxide is used as a substrate; under the condition that a biocatalyst, a cofactor and a cofactor regeneration system exist, the substrate performs the reduction reaction to generate 6-cyano-(3R, 5R)-dihydroxyhexanoate; and the reduction reaction is carried out in water-phase buffer solution with pH of 6 to 8. According to the invention, by optimizing the process condition and mainly optimizing the pH of the reduction reaction, the concentration of the substrate is improved and the enzyme dosage is reduced, so that production cost is reduced and the high-efficiency biological conversion process is realized.

The invention relates to a method for efficiently saccharifying biomass wastes such as xylose residue or furfural residue, comprising the following steps: performing steam exploding processing on biomass wastes such as xylose residue or furfural residue, then collecting materials after steam exploding, carrying out solid-liquid separation, and performing enzymolysis and saccharification on a solid phase obtained from separation. In the method of the invention, the crystallizing structure of cellulose is destroyed by steam exploding processing, the specific surface area of cellulose is increased, and the content of lignin is reduced, thereby reducing the use amount of cellulase during saccharification of lignocellulose materials, improving the enzymolysis efficiency, increasing sugar concentration in the enzymolysis solution, effectively reducing the cost for enzymolysis and sugar concentration utilizing lignocellulose materials, promoting industrialized recover and reuse to the waste, and being favorable for protecting the environment and saving resources.

The invention discloses a method for synthesizing medium-long-chain structure ester by an enzymic method. Crushed immobilized lipase is used as a catalyst; long-chain triglyceride and medium-chain triglyceride are fed according to a mass ratio of 6.4 to (2 to 10); the reaction temperature is 45 to 80 DEG C; the stirring reaction is performed for 1 to 10h in the vacuum state. When the method is used for preparing the medium-long-chain structure ester, the enzyme consumption can be reduced; the reaction time is shortened; the generation quantity of byproducts such as diglyceride is reduced; theproduct acid value is reduced; the high target product yield is maintained, so that the target product production cost is reduced; the economic benefits are improved.

The invention relates to a casein phosphopept continuous production process by adopting an immobilized enzyme hydrolysis crude cheese and ultrafiltration membrane separation method, comprising the following steps: a. extraction of tryptase; b. preparation of tryptase immobilized reaction column; c. crude cheese hydrolysis; d. separation and purification of the casein phosphopept (CPP) with an ultrafiltration membrane; e. column chromatography of CPP ultrafiltrate. The process has rational design, strong operability, short production process, high concentration of intermediate products and low production energy consumption; the main separation and purification processes are carried out at normal temperature, the evaporation capacity is less in the drying process, the energy consumption in the production can be greatly reduced, and the cost is saved; the product is obtained by vacuum concentration and spray drying, the main ingredient of the product is micromolecule bioactive polypeptides, the polypeptide with 2000-6000 of molecular weight accounts for more than 45 percent of the polypeptides.

An enzymic preparation method of a luliconazole intermediat is characterized in that 2-chlorine-1-(2, 4-dichlorophenyl) acetone is used as a substrate and is subjected to asymmetric reduction in the existence of ketoreductase, a cofactor and a hydrogen donor to obtain (S)-2-chlorine-1-(2, 4-dichlorophenyl) ethyl alcohol. The method comprises the following steps: (1) preparing ketoreductase, namely, inoculating single recombinant escherichia coli containing ketoreductase gene into liquid LB culture medium with kanamycin resistance; culturing under temperature of 37 DEG C, and staying overweight; inoculating the activated culture material into the kanamycin containing liquid LB culture medium; oscillating and culturing under the temperature of 37 DEG C until OD600 is 0.6-0.8; adding IPTG with final concentration of 0.1M; performing induced culture for 8-10h under the temperature of 25 DEG C; centrifuging; collecting thallus; and ultrasonically breaking walls to obtain the recombinant ketoreductase; and (2) preparing (S)-2-chlorine-1-(2, 4-dichlorophenyl) ethyl alcohol, namely, adding the substrate 2-chlorine-1-(2, 4-dichlorophenyl) acetone to a buffering solution, and adding certainamount of cofactor NAD(P) and isopropanol; adding the recombinant ketoreductase; reacting by biologically catalyzing under the temperature of 30 DEG C; and adding ethyl acetate for extracting after the reaction is finished, thus obtaining the (S)-2-chlorine-1-(2, 4-dichlorophenyl) ethyl alcohol.

In view of problems in the existing cellulose ethanol preparation technologies that inhibitors in a cellulose hydrolyzate inhibit fermentation, xylose cannot be completely converted, glucose inhibitsfermentation of the xylose, and a cellulase acting temperature and a microbe fermentation temperature are non-uniform, the invention provides a fermentation method for producing xylitol and ethanol byusing undetoxified cellulose raw materials. The fermentation method comprises the steps of inoculum culture, xylose fermentation, cellulase adding, synchronous diastatic fermentation and the like. According to the method, simultaneous utilization of the xylose and the inhibitors is achieved by adopting an acetic acid and xylose co-utilization based Kluyveromyces marxianus strain, and thus, the problem in inhibitor removal is avoided; by adopting high-temperature synchronous diastatic fermentation, the problem that the cellulase is unmatched with a fermentation temperature is avoided; and compared with the existing cellulose ethanol fermentation technologies, the production cost can be remarkably reduced.

The invention provides a method for extracting peanut oil and peanut butter through an aqueous enzymatic method and belongs to the technical field of vegetable oil and fat processing. The method includes the following steps that 1, peanuts are subjected to extrusion and puffing processing after being peeled and crushed, and a puffing product is obtained; 2, water is added into an enzymolysis reaction kettle, after steam heating is conducted, the puffing product is added, mixed liquid is obtained, after the pH of the mixed liquid is adjusted, alkaline protease is added for enzymolysis, after enzymolysis is conducted, a horizontal centrifuge is adopted for conducting centrifugation, and a liquid phase and residuals are obtained; 3, a disk centrifuge is adopted for conducting centrifugation on the liquid phase, the peanut oil and the water phase are obtained, the residuals are mixed with water after being crushed, mixed slurry is obtained, and the mixed slurry is subjected to heat treatment; 4, the mixed slurry obtained after heat treatment is placed into a blending tank, then saccharose, salt, essence and agar are added for blending, the blended slurry is subjected to homogeneity, concentration and sterilization, and the peanut butter is obtained. The method is simple in process, low in cost and suitable for industrialized continuous production, and the high-quality peanut oil and the low-fat high-protein peanut butter can be obtained at the same time.

The invention relates to a biological preparation method of (R)-4-cyano-hydroxybutanoate. According to the biological preparation method, (S)-4-chlorine-3-hydroxybutanoate is used as a substrate; under the action of a biocatalyst, the substrate reacts with sodium cyanide to generate a target product (R)-4-cyano-hydroxybutanoate; the biocatalyst is recombination halohydrin dehalogenase; and the reaction is carried out in a water phase with the pH of 6 to 8. According to the invention, by adopting the recombination halohydrin dehalogenase, the concentration of the substrate is improved and the enzyme dosage is reduced, so that production cost is reduced. Moreover, by optimizing the process, both purity and yield of the product are improved.

Disclosed herein is a process for production of an instant coffee product in a high yield, comprising the steps of extracting roast and ground coffee beans with water having a temperature of 80° C. or less, to produce a first extract and spent coffee grounds, adding water to the spent coffee grounds to produce an aqueous suspension, hydrolysing the aqueous suspension using a hydrolysing enzyme to produce a second extract and spent remains, adding the first extract to the second extract, optionally after concentration and/or drying of the second extract, to obtain a combined extract, and drying the combined extract to obtain an instant coffee product. The high yield is obtained due to the reduction of enzyme inhibiting substances.