Casein phosphopept continuous production process by adopting immobilized enzyme hydrolysis crude cheese and ultrafiltration membrane separation method

A casein phosphopeptide, immobilized enzyme technology, applied in protein food processing, animal protein processing, protein food ingredients and other directions, can solve the problems of affecting the flavor and quality of the final product, difficult to industrialize production, expensive carrier, etc. Fixed cost, improved utilization rate, high product purity effect

Active Publication Date: 2010-06-30
甘南州科瑞乳品开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Its disadvantages are: using chitosan as the carrier, the carrier is expensive, the cost is high, and it is not easy for industrial production
However, the disadvantage of this method is that the hydrolysis process is carried out in one reaction kettle, which may easily cause multiple hydrolysis of the substrate to produce small peptides, which brings difficulties for subsequent separation and affects the flavor and quality of the final product.

Method used

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  • Casein phosphopept continuous production process by adopting immobilized enzyme hydrolysis crude cheese and ultrafiltration membrane separation method
  • Casein phosphopept continuous production process by adopting immobilized enzyme hydrolysis crude cheese and ultrafiltration membrane separation method

Examples

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Embodiment 1

[0031] A method for continuous production of casein phosphopeptides by hydrolyzing crude cheese with immobilized enzymes and ultrafiltration membrane separation, the steps of which are divided into:

[0032] a. Extraction of trypsin Take 10kg of fresh bovine pancreas, remove the fat and connective tissue manually, grind it with a meat grinder and immediately immerse it in a 50L interlayer reaction tank with 1L of 0.1mol / L ammonium sulfate solution at 0℃~4℃ After stirring, it becomes a slurry, add 0.1mol / L ammonium sulfate twice the volume of the slurry, extract in a jacketed reaction tank at 0°C to 4°C for 20 hours, and slowly stir every half hour. Filter through an air filter press, collect the filtrate, and repeat the leaching of the residue once with 0.1 mol / L ammonium sulfate 1 times the volume of the slurry, combine the two filtrates, and discard the residue.

[0033] Add 0.2kg / L solid ammonium sulfate to the filtrate in the 50L interlayer reaction tank at 0°C to 4°C to a...

Embodiment 2

[0066] a. Extraction of trypsin

[0067] Take 100kg of fresh bovine pancreas, manually remove fat and connective tissue, grind it with a meat grinder, and immediately immerse it in a 500L interlayer reaction tank with 10L of 0.1mol / L ammonium sulfate solution at 0°C to 4°C. After stirring, it becomes For the slurry, add 0.1 mol / L ammonium sulfate 3 times the volume of the slurry and extract in a jacketed reaction tank at 0°C to 4°C for 30 hours, stirring slowly every half hour. Filter through an air filter press, collect the filtrate, and repeat the leaching of the residue once with 0.1 mol / L ammonium sulfate 1 times the volume of the slurry, combine the two filtrates, and discard the residue.

[0068] Add 0.2kg / L solid ammonium sulfate to the filtrate in the 500L interlayer reaction tank at 0°C to 4°C to a concentration of 1.5mol / L, stir and let it stand for 10 hours, filter and remove the precipitate. Continue to add 0.27kg / L solid ammonium sulfate to the filtrate to a conc...

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Abstract

The invention relates to a casein phosphopept continuous production process by adopting an immobilized enzyme hydrolysis crude cheese and ultrafiltration membrane separation method, comprising the following steps: a. extraction of tryptase; b. preparation of tryptase immobilized reaction column; c. crude cheese hydrolysis; d. separation and purification of the casein phosphopept (CPP) with an ultrafiltration membrane; e. column chromatography of CPP ultrafiltrate. The process has rational design, strong operability, short production process, high concentration of intermediate products and low production energy consumption; the main separation and purification processes are carried out at normal temperature, the evaporation capacity is less in the drying process, the energy consumption in the production can be greatly reduced, and the cost is saved; the product is obtained by vacuum concentration and spray drying, the main ingredient of the product is micromolecule bioactive polypeptides, the polypeptide with 2000-6000 of molecular weight accounts for more than 45 percent of the polypeptides.

Description

technical field [0001] The present invention relates to food product processing technology. Background technique [0002] Casein Phosphopeptides (CPP) is a natural physiologically active peptide containing phosphoserine clusters obtained from bovine casein as raw material, hydrolyzed by a single or complex protease, separated and purified from the hydrolyzed product, and dried. [0003] The core part of CPP is -SerP-SerP-SerP-Glu-Glu-, which has a strong activity of promoting the absorption of calcium and other mineral elements, and can combine with divalent and some trivalent mineral ions such as calcium, iron, zinc, etc. It can be used not only as a carrier of calcium, but also as a carrier of iron, manganese, zinc, and selenium. Therefore, CPP has been recognized as a widely used functional food additive. Health care for the elderly. [0004] Gu Kunrui and others introduced the experimental study of immobilized trypsin and its use in the preparation of casein phosphopep...

Claims

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Application Information

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IPC IPC(8): A23J3/10A23J3/34
Inventor 胡先望张瑞贞陈朋梁宁
Owner 甘南州科瑞乳品开发有限公司
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