36results about How to "Improve the conversion rate of enzymatic hydrolysis" patented technology

The invention discloses a combined pretreatment method of lignocellulose. Lignocellulose is pulverized and loaded into a circulation reactor, and dilute acid is injected, and the circulation pump is turned on to carry out a circulation reaction at a temperature of 50°C to 200°C. The reaction After the end, the obtained hydrolyzate is deacidified and used for fermentation; after the remaining lignocellulose in the circulation reactor is washed, it is put into a ball mill and added with lye for ball milling. After the ball milling is completed, it is used for subsequent enzymatic hydrolysis. The invention can effectively improve the conversion rate of hemicellulose in lignocellulose, the removal rate of lignin, the conversion rate of cellulose enzymolysis, and reduce the fermentation inhibitory products. Concentration, and the water consumption of pretreatment can be set according to the needs of subsequent fermentation concentration.

A disclosed method for obtaining high-purity hesperetin from valeriana jatamansi residue comprises: heating valeriana jatamansi residue from which total terpenoid is extracted, leaching for 2 times, adjusting pH of the leaching solution, utilizing macroporous adsorption resin column for gathering hesperetin, firstly eluting with deionzed water, when the eluate is cleared, then eluting with a sodium hydroxide solution with a mass concentration of about 1%, gathering the eluate, adjusting the pH value of the eluate, and performing enzymatic hydrolysis, centrifugation filtering and recrystallization to obtain high-purity hesperetin which is light yellow acicular crystal. The method is simple in operation, low in production cost and high in source utilization rate, and is easy for industrialized production.

The invention relates to a comprehensive utilization method of lignocellulose biomass. The method comprises the following steps of (a) performing acid hydrolysis on the lignocellulose biomass to obtain a pentose solution and acid hydrolysis residues after separation; (b) treating the acid hydrolysis residues with an alkali solution so as to extract alkali lignin; and (c) performing enzymatic hydrolysis on the alkali hydrolysis residues obtained by alkali solution treatment in the step (b) by using cellulose so as to obtain a solution with glucose as a main component, wherein the cellulose is obtained by culturing a strain of penicillium, the penicillium is named as penicillium recumbent PD-G3-08 which is preserved in Wuhan University, China Center for Type Culture Collection, with a preservation number of CCTCC M 2011195. The above method increases conversion rate of cellulose enzymatic hydrolysis and realizes comprehensive utilization of the lignocellulose biomass resources.

The invention discloses a method for extracting and separating kaempferol from Ligustrum sinense branches and leaves, which comprises the following steps: carrying out thermal-soak extraction on Ligustrum sinense twice, regulating the pH value, enriching with macroporous adsorbent resin, and flushing the columns with deionized water flushing liquid; after the effluent liquid becomes clear, eluting the flushed column filler with 0.8-1 wt% sodium hydroxide eluting liquid, and collecting the eluate; and regulating the pH value of the eluate, carrying out enzymolysis, crystallizing, filtering, and recrystallizing multiple times, thereby obtaining the yellow acicular crystal kaempferol refined product. The method has the advantages of low production cost and wide industrialization prospects, is simple to operate and can easily implement industrial production.

The invention relates to an enzymatic hydrolysis product of total flavonoids of Epimedium, which is produced by enzymatic hydrolysis of total flavonoids of Epimedium through immobilized snail enzymes, and the enzymatic hydrolysis product has higher Better anti-tumor activity and better oral absorption. The invention also discloses a preparation method of the enzymatic hydrolysis product. The method uses immobilized enzymes to enzymolyze the total flavonoids of Epimedium herba, and the selected helicase has high binding efficiency, good stability, and high enzymatic hydrolysis conversion rate with the immobilized carrier. Moreover, the immobilized enzyme can also be recycled, which can save resources and realize green production. The present invention also relates to the application of the enzymatic hydrolyzate in anti-tumor. The total flavonoid enzymatic hydrolyzate of Epimedium herba obtained according to the technical scheme provided by the present invention is effective against human lung cancer A549 cells, liver cancer HepG2 cells, breast cancer MCF‑7 cells, cervical cancer Hela cells have significant proliferation inhibitory effect and can be used for the treatment of lung cancer, liver cancer, breast cancer and cervical cancer.

The invention provides a method for promoting poplar ethanol production by pretreatment of hydrothermal combined with NaOH aqueous solution. In the present invention, the poplar is first crushed, reacted with water at 180-200 DEG C for 5-30 minutes, the separated filter residue is mixed with NaOH aqueous solution, reacted at 80-120 DEG C for 30-120 minutes, and the pretreatment residue is obtained by separation, Then add cellulase, yeast activation solution and nutrient solution for saccharification and fermentation. The method adopts the combination of two stages of pretreatment of hydrothermal and NaOH aqueous solution to reduce the influence of lignin on the enzymatic hydrolysis and fermentation process, and under the premise of reducing the alkali concentration, it can also effectively promote the production of ethanol from poplar, and the ethanol yield is as high as 50.91%.

A comprehensive utilization method of lignocellulose biomass comprises the following steps: (a) performing alkaline hydrolysis of the lignocellulose biomass to extract lignin; (b) performing acid hydrolysis of the residues obtained in the alkaline hydrolysis processing , separating to obtain a pentose solution and acid hydrolysis residues; (c) performing enzymatic hydrolysis of the residues obtained by alkaline hydrolysis processing in step (b) by using cellulase to obtain a solution with a main component of glucose, wherein the cellulase is obtained by culturing a strain of penicillium which has a classification name of Penicillium decumbens PD-G3-08, and is preserved in Wuhan University China Center for Type Culture Collection with a preservation number of CCTCC M 2011195 and a preservation date of June 13, 2011. The method improves the extraction ratio of cellulose enzymatic hydrolysis, and realizes comprehensive utilization of lignocellulose biomass resources.

A comprehensive utilization method of lignocellulose biomass comprises the following steps: (a) performing acid hydrolysis of the lignocellulose biomass, separating to obtain a pentose solution and acid hydrolysis residues; (b) processing the acid hydrolysis residues by using an alkaline solution so as to extract alkaline lignin; (c) performing enzymatic hydrolysis of the alkaline hydrolysis residues obtained by alkaline solution processing in step (b) by using cellulase to obtain a glucose solution and enzymatic hydrolysis residues; (d) after step (c) is complete, returning the obtained enzymatic hydrolysis residues to step (b) for alkaline solution processing, or combining the enzymatic hydrolysis residues with new acid hydrolysis residues and performing alkaline solution processing in step (b), then orderly performing steps (c) and (d), cycling the steps so as to further extract lignin and to perform enzymatic hydrolysis of the celluose. The method improves the conversion rate of cellulose enzymatic hydrolysis, and realizes comprehensive resource utilization of lignocellulose biomass.

A comprehensive utilization method of lignocellulose biomass comprises the following steps: (a) performing N-stage acid hydrolysis of lignocellulose biomass, wherein the concentrations of the acid solutions decrease gradually from the first stage to the Nth stage by 10-40% of the concentration of the first stage acid solution,, and N is an integer more than or equal to 2; (b) processing the acid hydrolysis residues by using an alkaline solution so as to extract alkaline lignin; (c) performing enzymatic hydrolysis of the alkaline hydrolysis residues obtained by alkaline solution processing in step (b) by using cellulase to obtain a glucose solution and enzymatic hydrolysis residues; (d) after step (c) is complete, returning the obtained enzymatic hydrolysis residues to step (b) for alkaline solution processing, or combining the enzymatic hydrolysis residues with new acid hydrolysis residues and performing alkaline solution processing in step (b), then orderly performing steps (c) and (d), cycling the steps so as to further extract lignin and to perform enzymatic hydrolysis of the celluose. The method realizes the maximum resource utilization of lignocellulose biomass.

The invention discloses a method for preprocessing lignocellulose, which comprises: installing the lignocellulose into a recycle reactor, injecting diluted acid, opening a circulating pump to carry out cyclic reaction under the temperature of 50DEG C-200DEG C, acid-removing and filtering hydrolyzate which is obtained after finishing the reaction, and using the hydrolyzate to ferment. The inventionalso discloses a system which is applied in the method. The method of the invention can effectively increase the conversion rate of hemicellulose and the conversion rate of cellulase hydrolysis in the lignocellulose, fermentation inhibiting products are reduced, simultaneously, the time for preprocessing also can be shortened, and the energy consumption is lowered.