36 results about "Penicillium decumbens" patented technology

The present invention provides for heterologous expression of beta-glucosidase (BGL) polypeptides encoded by Humicola grisea, Candida wickerhamii, Aspergillus aculeatus, Aspergillus oryzae, Penicillium decumbens, Chaetomium globosum, Neocallimastix frontalis, Debaryomyces hansenii, Kluyveromyces marxianus, or Phytophthora infestans in host cells, such as the yeast Saccharomyces cerevisiae. The expression in such host cells of the corresponding genes, and variants and combinations thereof, result in improved specific activity of the expressed BGL. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.

The invention discloses a compound microorganism live bacteria preparation for degrading papermaking wastewater COD, a preparation method and applications thereof. The preparation contains Penicillium decumbens, Myrothecium Verrucaria, Bacillus massiliensis and a solid fermentation medium. The preparation method comprises the following steps: first, Penicillium decumbens and Myrothecium Verrucaria after inclined plane activation are inoculated in a liquid medium respectively, and cultured at the temperature of 25 DEG C -30 DEG C for 44-52 h to obtain a microorganism bacteria liquid; Bacillus massiliensis after inclined plane activation is inoculated in a liquid medium, and cultured at the temperature of 28 DEG C-37 DEG C for 40-52 h to obtain a microorganism bacteria liquid; second, the microorganism bacteria liquids are mixed uniformly, placed in a solid fermentation medium and fermented and cultured at the temperature of 30 DEG C for 40-80 h, and the processes of drying at the temperature of 40 DEG C-60 DEG C, crushing to 15-50 meshes and sieving are performed. The preparation can degrade papermaking wastewater COD effectively, rapidly and efficiently in applications as a COD-degrading enhancer in the papermaking wastewater treatment process. The preparation can improve wastewater chromaticity and water quality.

The invention discloses a method for increasing the fermentation yield of epothilone by using the metabolin of competitive microorganism namely rhizopus arrhizus or penicillium decumbens of sorangium cellulosum to induce. The method provided by the invention comprises the following steps of: cultivating the competitive microorganism, and extracting the metabolin of the competitive microorganism; adding the metabolin into a sorangium cellulosum fermentation medium, activating epothilone to produce related regulator genes inside the strain by using the stimulation of the external additive, and further stimulating the synthesis of epothilone. Detection results show that the fermentation yield of epothilone can be increased by 46.3-58.7% as compared with original conditions after the method is used, and the method has important theoretical significance and economic value.

A comprehensive utilization method of lignocellulose biomass comprises the following steps: (a) performing acid hydrolysis of the lignocellulose biomass, separating to obtain a pentose solution and acid hydrolysis residues; b) performing enzymatic hydrolysis of the acid hydrolysis residues in step (a) by using cellulase, then performing fermentation to produce ethanol, wherein the cellulase is obtained by culturing a strain of penicillium which has a classification name of Penicillium decumbens PD-G3-08, and is preserved in Wuhan University China Center for Type Culture Collection with a preservation number of CCTCC M 2011195; (c) processing the fermentation residues produced in step (b) by using an alkaline solution so as to extract lignin from the fermentation residues; (d) after step (c) is complete, returning to step (b) to perform enzymatic hydrolysis and fermentation by using residues obtained after alkaline solution processing as enzymatic hydrolysis raw materials, and then performing steps (c) and (d). The method realizes the maximum resource utilization of lignocellulose biomass.

A comprehensive utilization method of lignocellulose biomass comprises the following steps: (a) performing alkaline hydrolysis of the lignocellulose biomass to extract lignin; (b) performing acid hydrolysis of the residues obtained in the alkaline hydrolysis processing , separating to obtain a pentose solution and acid hydrolysis residues; (c) performing enzymatic hydrolysis of the residues obtained by alkaline hydrolysis processing in step (b) by using cellulase to obtain a solution with a main component of glucose, wherein the cellulase is obtained by culturing a strain of penicillium which has a classification name of Penicillium decumbens PD-G3-08, and is preserved in Wuhan University China Center for Type Culture Collection with a preservation number of CCTCC M 2011195 and a preservation date of June 13, 2011. The method improves the extraction ratio of cellulose enzymatic hydrolysis, and realizes comprehensive utilization of lignocellulose biomass resources.

The invention discloses a penicillium decumbens engineered strain containing over-expressed xylanase, which contains over-expressed penicillium decumbens xylanase I genes, is named penicillium decumbens X8 and is preserved in China Centre for Type Culture Collection on May 7, 2010 with a preservation number of CCTCC M 2010110. The invention also discloses the application of the strain in production of xylanase. The highest activity of the xylanase of the engineered strain of the invention reaches 219.82 IU / ml and is 1.57 times as high as that of an original host strain, which indicates that the strain is widely applied to the production of the xylanase.