35 results about "Penicillium decumbens" patented technology

The invention belongs to the field of microorganism, and particularly relates to a microorganism penicillium strain producing high-activity cellulase. The penicillium strain has a classification name of Penicillium decumbens PD-G3-08, and is preserved in Wuhan University China Center for Type Culture Collection with a preservation number of CCTCC M2011195. The invention also provides an application of the strain in cellulose enzymatic hydrolysis. Through mutation breeding, the penicillium strain producing high-activity cellulase is bred; cellulase crude enzyme liquid is obtained through fermentation of the strain, which has filter paper enzyme activity of up to 10 IU / ml, endoglucanase activity of up to 30 IU / ml, exoglucanase activity of up to 1.5 IU / ml and beta-glucosidase activity of up to 8 IU / ml, respectively being 3 times, 4 times, 4.5 times, and 4 times higher than the activities of an original strain; when the strain is applied to enzymatic hydrolysis of cellulose raw materials, the cellulose conversion rate in 3 days is up to more than 80%, and the cellulase has extremely high activity.

The invention discloses a method for preparing hesperetin from enzymatic hydrolysis neohesperidin or hesperidin. Neohesperidin or hesperidin beta-D-glucoside bond can be hydrolyzed in one step through crude enzyme liquid to prepare corresponding aglycone hesperetin. The method includes the steps of preparing the crude enzyme liquid of Penicillium decumbens through fermentation, adjusting the content and reaction time of the crude enzyme liquid, directly converting neohesperidin or hesperidin into hesperetin, dissolving or extracting conversion liquid through organic solvent, and conducting chromatographic purification to obtain hesperetin. By means of the method, the defects that a traditional chemical method is strict in reaction condition, serious in pollution, low in conversion rate and the like when used for preparing hesperetin can be overcome, and the defects that when microbial fermentation is directly adopted, operation is complicated, products are not simplex, and products are difficult to purify can be overcome.

The invention belongs to the field of microorganism, and particularly relates to a microorganism penicillium strain producing high-activity cellulase. The penicillium strain has a classification name of Penicillium decumbens PD-G3-08, and is preserved in Wuhan University China Center for Type Culture Collection with a preservation number of CCTCC M2011195. The invention also provides an application of the strain in cellulose enzymatic hydrolysis. Through mutation breeding, the penicillium strain producing high-activity cellulase is bred; cellulase crude enzyme liquid is obtained through fermentation of the strain, which has filter paper enzyme activity of up to 10 IU / ml, endoglucanase activity of up to 30 IU / ml, exoglucanase activity of up to 1.5 IU / ml and beta-glucosidase activity of up to 8 IU / ml, respectively being 3 times, 4 times, 4.5 times, and 4 times higher than the activities of an original strain; when the strain is applied to enzymatic hydrolysis of cellulose raw materials, the cellulose conversion rate in 3 days is up to more than 80%, and the cellulase has extremely high activity.

An animal feed additive comprises the following components in percentage by weight: 25-29% of lactobacillus, 24-28% of saccharomyces cerevisiae, 33-37% of penicillium decumbens and 10-14% of aspergillus niger. A preparation process comprises the following steps: (1) placing the four strains into four fermentation tanks respectively; (2) placing vectors and nutrients with addition proportion of 3%into each fermentation tank in which the strains account for 97%; (3) controlling the temperature of the fermentation tanks between 25 DEG C and 35 DEG C and humidity between 60% and 70%, and carryingout fermentation under the acid environment with pH value less than 7; (4) taking out the solid obtained after fermentation in each fermentation tank, baking and then processing the solid into powder; and (5) mixing the four kinds of powder. The animal feed additive is prepared by mixing the microbial strains, has all-round nutrition and has no effect on the quality of animals. The preparation process is simple and the cost is lowered.

The invention discloses a method for increasing the fermentation yield of epothilone by using the metabolin of competitive microorganism namely rhizopus arrhizus or penicillium decumbens of sorangium cellulosum to induce. The method provided by the invention comprises the following steps of: cultivating the competitive microorganism, and extracting the metabolin of the competitive microorganism; adding the metabolin into a sorangium cellulosum fermentation medium, activating epothilone to produce related regulator genes inside the strain by using the stimulation of the external additive, and further stimulating the synthesis of epothilone. Detection results show that the fermentation yield of epothilone can be increased by 46.3-58.7% as compared with original conditions after the method is used, and the method has important theoretical significance and economic value.

A comprehensive utilization method of lignocellulose biomass comprises the following steps: (a) performing acid hydrolysis of the lignocellulose biomass, separating to obtain a pentose solution and acid hydrolysis residues; b) performing enzymatic hydrolysis of the acid hydrolysis residues in step (a) by using cellulase, then performing fermentation to produce ethanol, wherein the cellulase is obtained by culturing a strain of penicillium which has a classification name of Penicillium decumbens PD-G3-08, and is preserved in Wuhan University China Center for Type Culture Collection with a preservation number of CCTCC M 2011195; (c) processing the fermentation residues produced in step (b) by using an alkaline solution so as to extract lignin from the fermentation residues; (d) after step (c) is complete, returning to step (b) to perform enzymatic hydrolysis and fermentation by using residues obtained after alkaline solution processing as enzymatic hydrolysis raw materials, and then performing steps (c) and (d). The method realizes the maximum resource utilization of lignocellulose biomass.

The present invention provides for heterologous expression of beta-glucosidase (BGL) polypeptides encoded by Humicola grisea, Candida wickerhamii, Aspergillus aculeatus, Aspergillus oryzae, Penicillium decumbens, Chaetomium globosum, Neocallimastix frontalis, Debaryomyces hansenii, Kluyveromyces marxianus, or Phytophthora infestans in host cells, such as the yeast Saccharomyces cerevisiae. The expression in such host cells of the corresponding genes, and variants and combinations thereof, result in improved specific activity of the expressed BGL. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.

The invention discloses a penicillium decumbens engineered strain containing over-expressed xylanase, which contains over-expressed penicillium decumbens xylanase I genes, is named penicillium decumbens X8 and is preserved in China Centre for Type Culture Collection on May 7, 2010 with a preservation number of CCTCC M 2010110. The invention also discloses the application of the strain in production of xylanase. The highest activity of the xylanase of the engineered strain of the invention reaches 219.82 IU / ml and is 1.57 times as high as that of an original host strain, which indicates that the strain is widely applied to the production of the xylanase.

The invention discloses a compound microorganism live bacteria preparation for degrading papermaking wastewater COD, a preparation method and applications thereof. The preparation contains Penicillium decumbens, Myrothecium Verrucaria, Bacillus massiliensis and a solid fermentation medium. The preparation method comprises the following steps: first, Penicillium decumbens and Myrothecium Verrucaria after inclined plane activation are inoculated in a liquid medium respectively, and cultured at the temperature of 25 DEG C -30 DEG C for 44-52 h to obtain a microorganism bacteria liquid; Bacillus massiliensis after inclined plane activation is inoculated in a liquid medium, and cultured at the temperature of 28 DEG C-37 DEG C for 40-52 h to obtain a microorganism bacteria liquid; second, the microorganism bacteria liquids are mixed uniformly, placed in a solid fermentation medium and fermented and cultured at the temperature of 30 DEG C for 40-80 h, and the processes of drying at the temperature of 40 DEG C-60 DEG C, crushing to 15-50 meshes and sieving are performed. The preparation can degrade papermaking wastewater COD effectively, rapidly and efficiently in applications as a COD-degrading enhancer in the papermaking wastewater treatment process. The preparation can improve wastewater chromaticity and water quality.

The invention relates to a method for preparing a protein feed by mixing straw and straw dilute acid hydrolytic pentose butanol or alcoholic fermentation waste water for solid state fermentation, which comprises the following steps of: mixing the steam-exploded straw or the straw hydrolyzed by dilute acid and the straw dilute acid hydrolytic pentose butanol or alcoholic fermentation waste water in a certain volume to ensure that moisture content is between 70 and 80 percent, after sterilizing, inoculating penicillium decumbens and trametes pubescens to the mixture and performing aerobic solid state fermentation for 4 to 6 days, inoculating candida tropicalis and lactobacillus plantarum and performing anaerobic fermentation for 2 to 3 days, and drying to obtain the protein feed. In the protein feed product prepared by the method, the protein content is between 22 and 27 percent, and the degradation rate of coarse fibers is between 63 and 68 percent. The method can solve the problem of discharging the butanol or alcoholic fermentation waste water; and the waste water and the straw are converted into the protein feed, so that the waste is turned into treasure.

The invention discloses a method for increasing the fermentation yield of epothilone by using the metabolin of competitive microorganism namely rhizopus arrhizus or penicillium decumbens of sorangium cellulosum to induce. The method provided by the invention comprises the following steps of: cultivating the competitive microorganism, and extracting the metabolin of the competitive microorganism; adding the metabolin into a sorangium cellulosum fermentation medium, activating epothilone to produce related regulator genes inside the strain by using the stimulation of the external additive, and further stimulating the synthesis of epothilone. Detection results show that the fermentation yield of epothilone can be increased by 46.3-58.7% as compared with original conditions after the method is used, and the method has important theoretical significance and economic value.

The invention discloses a penicillium deumbens engineered strain containing over-expressed beta-glucosidase, which contains over-expressed beta-glucosidase genes, is named penicillium deumbens Gi31-2 and is preserved in China Center for Type Culture Collection on May 7, 2010 with a preservation number of CCTCC M 2010111. The invention also discloses the application of the strain in production of the beta-glucosidase and degradation of cellulose. The highest activity of the beta-glucosidase of the engineered strain of the invention reaches 3.47 IU / ml and is 2.90 times as high as that of an original host strain, while highest filter paper activity is 3.32 IU / ml and is 1.23 times as high as that of the original host strain. Therefore, the strain is widely applied to the production of the beta-glucosidase.

A comprehensive utilization method of lignocellulose biomass comprises the following steps: (a) performing acid hydrolysis of the lignocellulose biomass, separating to obtain a pentose solution and acid hydrolysis residues; b) performing enzymatic hydrolysis of the acid hydrolysis residues in step (a) by using cellulase, then performing fermentation to produce ethanol, wherein the cellulase is obtained by culturing a strain of penicillium which has a classification name of Penicillium decumbens PD-G3-08, and is preserved in Wuhan University China Center for Type Culture Collection with a preservation number of CCTCC M 2011195; (c) processing the fermentation residues produced in step (b) by using an alkaline solution so as to extract lignin from the fermentation residues; (d) after step (c) is complete, returning to step (b) to perform enzymatic hydrolysis and fermentation by using residues obtained after alkaline solution processing as enzymatic hydrolysis raw materials, and then performing steps (c) and (d). The method realizes the maximum resource utilization of lignocellulose biomass.

The invention relates to a strain of Penicillium decumbens UC086 capable of effectively transforming dehydroepiandrosterone (DHEA) into 1alpha-OH-DHEA, and a culture method thereof and application thereof in transforming steroid medicines, and belongs to the technical field of microorganisms. The strain was preserved in China General Microbiological Culture Collection Center on August 9, 2010 with the collection number of GCMCC NO.4075. The strain has the characteristics that the strain is quickly grown and easy to culture, and can efficiently transform the DHEA into the 1alpha-OH-DHEA; moreover, the product is easy to extract, and the final product has high purity. Through detection, the biological conversion rate of the 1alpha-OH-DHEA is 20 to 50 percent, the product extraction yield is 75 to 80 percent, and the purity of the 1alpha-OH-DHEA product is over 99.5 percent.

The invention relates to a new technical method for increasing the enzyme activity of cellulase. In the fermentation and production process of the cellulase produced from fungal, farnesol is added to increase the enzyme activity of cellulase. By adding farnesol, the filter paper enzyme activity of the Trichoderma reesei cellulase-producing is increased by about 30% and the filter paper enzyme activity of Penicillium decumbens cellulase-producing is increased by about 46%. The method in the invention is simple and practical, the effect of increasing the enzyme activity of cellulase is obvious;and the method can be widely used and can be used in various cellulase-producing industries adopting fungal fermentation.

The invention relates to an engonus fungus with high enzyme activity and high capacity of efficiently degrading straw cellulose in the northeast China region, in particular to a fungus for degrading cellulose. The engonus fungus with high enzyme activity and high capacity of efficiently degrading the straw cellulose in the northeast China region is named as Penicillium decumbens C5, belongs to the Penicillium sp., and has the preservation number of CGMSS No.4203 and the preservation date of October 8th 2010. When the Penicillium decumbens C5 strain is cultured for 3d, FPase reaches 7085.66U / L, the EG / Cen peak is 3856.97U / L, the CBU / Cex peak is 2681.59U / L, and the beta-Gase peak is 1487.86U / L, thus the Penicillium decumbens C5 has a function of efficiently degrading straws.

A comprehensive utilization method of lignocellulose biomass comprises the following steps: (a) performing alkaline hydrolysis of the lignocellulose biomass to extract lignin; (b) performing acid hydrolysis of the residues obtained in the alkaline hydrolysis processing , separating to obtain a pentose solution and acid hydrolysis residues; (c) performing enzymatic hydrolysis of the residues obtained by alkaline hydrolysis processing in step (b) by using cellulase to obtain a solution with a main component of glucose, wherein the cellulase is obtained by culturing a strain of penicillium which has a classification name of Penicillium decumbens PD-G3-08, and is preserved in Wuhan University China Center for Type Culture Collection with a preservation number of CCTCC M 2011195 and a preservation date of June 13, 2011. The method improves the extraction ratio of cellulose enzymatic hydrolysis, and realizes comprehensive utilization of lignocellulose biomass resources.

The invention discloses a penicillium decumbens engineered strain containing over-expressed xylanase, which contains over-expressed penicillium decumbens xylanase I genes, is named penicillium decumbens X8 and is preserved in China Centre for Type Culture Collection on May 7, 2010 with a preservation number of CCTCC M 2010110. The invention also discloses the application of the strain in production of xylanase. The highest activity of the xylanase of the engineered strain of the invention reaches 219.82 IU / ml and is 1.57 times as high as that of an original host strain, which indicates that the strain is widely applied to the production of the xylanase.

The invention relates to mannanases and a recombinant expression bacterial strain thereof. Expression genes of the mannanases is cloned from penicillium decumbens. Amino acid sequences of the mannanases are shown in the formula of SEQ ID NO: 1. The invention also comprises Pichia pastoris pdman-2 engineering bacteria and Trichoderma reesei pdman-2 engineering bacteria for expression of the mannanases. The novel expression genes of the mannanases are cloned from penicillium decumbens, and the Pichia pastoris pdman-2 engineering bacteria and the Trichoderma reesei pdman-2 engineering bacteria for expression of the novel expression gene are constructed. The mannanase expressed by the Pichia pastoris pdman-2 engineering bacteria has the most suitable pH value of 4.5, the most suitable temperature of 55 DEG C and stable enzyme activity in a pH value of 2.0-7.0, and can resist gastric acid and pepsin. The mannanase expressed by the Trichoderma reesei pdman-2 engineering bacteria has the most suitable pH value of 4.5, the most suitable temperature of 55 DEG C and stable enzyme activity in a pH value of 3.5-8.0, and can resist trypsin. The mannanases have feeding-enzyme application values.