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Penicillium decumbens, culture method thereof, and application thereof in transforming steroid medicines

A technology of penicillium recumbentum and a culture method, applied in the field of microorganisms, can solve the problems of difficulty in oxygen atoms, poor selectivity, etc., and achieve the effects of high purity of the final product, improved conversion rate, and easy extraction.

Active Publication Date: 2013-03-13
SHANDONG BAIZAOGANGMU BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The biggest limitation of the chemical synthesis method applied to the hydroxylation of steroidal compounds is its poor selectivity, especially the introduction of the oxygen atom necessary for steroidal activity at the C1α position of the steroidal mother nucleus is extremely difficult

Method used

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  • Penicillium decumbens, culture method thereof, and application thereof in transforming steroid medicines
  • Penicillium decumbens, culture method thereof, and application thereof in transforming steroid medicines
  • Penicillium decumbens, culture method thereof, and application thereof in transforming steroid medicines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The culture method of bacterial strain Penicillium decumbens (Penicillium decumbens) UC086, the steps are as follows:

[0046] (1) Get Penicillium decumbens UC086 of strain CGMCC4075 and inoculate it into the activated medium, culture conditions: temperature 27° C., cultivate for 4 days to obtain activated Penicillium decumbens UC086;

[0047] (2) Get the activated Penicillium decumbens UC086 and inoculate it in the seed culture medium, so that the bacterium concentration in the seed culture medium reaches 5×10 6 cells / mL, cultured at 25°C, 100r / min for 2 days, filtered, and obtained.

[0048] The activation medium components in the step (1) are as follows: potatoes (peeled): 200g, glucose: 20g, agar: 20g, water: 1000mL, and the pH value is natural.

[0049] The formula of the seed medium is glucose 3g / L, yeast extract powder 3g / L, magnesium sulfate 0.1g / L, pH 5.0.

[0050] The application of bacterial strain Penicillium decumbens UC086 in the production of 1α-hydroxy...

Embodiment 2

[0058] The culture method of bacterial strain Penicillium decumbens (Penicillium decumbens) UC086, the steps are as follows:

[0059] (1) Inoculate Penicillium decumbens UC086 with strain preservation number CGMCC 4075 in the activated medium, culture conditions: temperature 29°C, culture for 3 days to obtain activated Penicillium decumbens UC086;

[0060] (2) Get the activated Penicillium decumbens UC086 and inoculate it in the seed culture medium, so that the bacterium concentration in the seed culture medium reaches 5×10 7 cells / mL, cultured at 30°C, 150r / min for 3 days, filtered, and obtained.

[0061] The components of the activation medium in the step (1) are as follows: 200 g of potatoes (peeled), 20 g of glucose, 20 g of agar, 1000 mL of water, and a natural pH.

[0062] The formula of the seed medium is glucose 4.5g / L, yeast extract powder 3.5g / L, magnesium sulfate 1.2g / L, pH 7.0.

[0063] The application of bacterial strain Penicillium decumbens UC086 in the produc...

Embodiment 3

[0071] The culture method of bacterial strain Penicillium decumbens (Penicillium decumbens) UC086, the steps are as follows:

[0072] (1) Inoculate Penicillium decumbens UC086 with strain preservation number CGMCC 4075 into the activation medium, culture condition temperature is 28°C, and cultivate for 3 days to obtain activated Penicillium decumbens UC086;

[0073] (2) Get the activated Penicillium decumbens UC086 and inoculate it in the seed culture medium, so that the bacterium concentration in the seed culture medium reaches 1×10 8 cells / mL, cultured at 35°C, 300r / min for 4 days, filtered, and obtained.

[0074] The activation medium components in the step (1) are as follows: potatoes (peeled): 200g, glucose: 20g, agar: 20g, water: 1000mL, and the pH value is natural.

[0075] The seed medium formula is glucose 5g / L, yeast extract powder 5g / L, magnesium sulfate 2g / L, pH 9.0

[0076] The application of bacterial strain Penicillium decumbens UC086 in the production of 1α-h...

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Abstract

The invention relates to a strain of Penicillium decumbens UC086 capable of effectively transforming dehydroepiandrosterone (DHEA) into 1alpha-OH-DHEA, and a culture method thereof and application thereof in transforming steroid medicines, and belongs to the technical field of microorganisms. The strain was preserved in China General Microbiological Culture Collection Center on August 9, 2010 with the collection number of GCMCC NO.4075. The strain has the characteristics that the strain is quickly grown and easy to culture, and can efficiently transform the DHEA into the 1alpha-OH-DHEA; moreover, the product is easy to extract, and the final product has high purity. Through detection, the biological conversion rate of the 1alpha-OH-DHEA is 20 to 50 percent, the product extraction yield is 75 to 80 percent, and the purity of the 1alpha-OH-DHEA product is over 99.5 percent.

Description

technical field [0001] The invention relates to a bacterial strain Penicillium decumbens UC086 capable of effectively converting dehydroepiandrosterone into 1α-hydroxy dehydroepiandrosterone, its cultivation method and its application in transforming steroidal drugs, belonging to field of microbiology. Background technique [0002] Steroidal drugs have anti-infection, anti-virus and anti-tumor effects, and are widely used in the treatment of skin diseases, rheumatism, cardiovascular diseases, cancer, etc., and have become the second most consumed drugs in human medicine after antibiotics. The structure of steroid drugs is very complex and contains multiple asymmetric centers. In particular, its activity mainly depends on the substituents, and they are often substituents at specific positions, such as the hydroxyl groups at C1α and C11β positions and the C1 and C2 positions. double bond etc. At present, it is difficult to produce steroidal drugs by total synthesis. Usually,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P15/00C12R1/80
Inventor 刘新利赵林刘佩卉张妍妍
Owner SHANDONG BAIZAOGANGMU BIOTECH CO LTD
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