The invention discloses a culture solution for improving the development rate of in-vitro cultured pig blastocysts as well as application and a use method of the culture solution, and belongs to the technical field of
livestock embryo engineering. The invention discloses a culture solution for improving the development rate of in-vitro cultured pig blastocysts, which is characterized in that a PZM3 culture solution is used as a basic culture solution, and 5-25ng / mL of
tumor necrosis factor superfamily
cell factor TNFSF12 is added. According to the present invention, the pig
embryo needs to be subjected to in-vitro culture for 4 days in the TNFSF12-free basic culture solution, and then is transferred to the TNFSF12-containing basic culture solution so as to be continuously cultured for 2 days, such that the generation efficiency of the pig
blastocyst can be effectively improved, and the support is provided for the application of the pig in-vitro fertilization, the
somatic cell nuclear transfer, the
embryo genome selection and other embryo
engineering technologies in the agricultural breeding and the
human medicine;