The invention relates to a cervical exfoliated cell preservation solution with a pretreatment function and application, and in particular to a cervical exfoliated cell preservation solution which contains cell fixative, anticoagulant, PH buffer, humectant, osmotic pressure-maintaining agent, microbe inactivator, mucus digestion ingredient and red blood cell treatment ingredient. The preservation solution can effectively preserve cervical exfoliated cells, protect the integrity and fixity of cells and prevent the pycnosis, cytolysis and distending of cells; the mucus treatment capability and the blood cell disintegration capability are high; the cell preservation time is up to 30 days; the slide preparation effect is flat and even, and preserved cells can also be applied in the preparationof cell wax blocks, HPV virus detection and the like; the effective period of the preservation solution is two years under normal temperature, the microbe killing capability is high, and the health ofmedical technicians is effectively protected.