Cervical exfoliated cell preservation solution with pretreatment function and application

A technique for exfoliating cells and preservation solution, which is applied in the fields of application, preservation of human or animal body, preparation of test samples, etc. It can solve the problems of unfavorable popularization and promotion, many overlapping and clumping cells, and poor cell preservation effect.

Inactive Publication Date: 2018-08-17
南京福怡科技发展股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] 1. The mucus in the mucus sample affects the precipitation and absorption of cells, which may easily cause false negatives;
[0004] 2. Bloody samples were not processed thoroughly, and red blood cells interfered with interpretation;
[0005] 3. Cells dissolve or swell, and the cell preservation effect is

Method used

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  • Cervical exfoliated cell preservation solution with pretreatment function and application
  • Cervical exfoliated cell preservation solution with pretreatment function and application
  • Cervical exfoliated cell preservation solution with pretreatment function and application

Examples

Experimental program
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Effect test

Embodiment 1

[0026] The preservation solution in this embodiment contains the following components: absolute ethanol 28%, methanol 45%, anticoagulant 5%, pH buffer 15%, phenol 1%, sodium chloride 3%, formaldehyde 1%, mucus digestion Composition 1.2%, red blood cell lysate 0.8%.

[0027] In this embodiment, the application of the cell preservation solution and the preparation method are as follows:

[0028] (1) Take 15ml of preservation solution and put it in a 50ml preservation bottle for later use;

[0029] (2) After the gynecologist clinically collects the sampling swabs, place them in a container containing a preservation solution;

[0030] (3) The medical technician enters the sample number and information, shakes for 5 minutes, and stands still for 30 seconds;

[0031] (4) Transfer 6 ml of the cell solution to a disposable test tube; place the test tube in a centrifuge and centrifuge at 2000r / min for 5 minutes;

[0032] (5) Discard 1 / 2 of the supernatant and shake for 1 minute; pip...

Embodiment 2

[0036] The preservation solution in this embodiment contains the following components: absolute ethanol 35%, methanol 30%, anticoagulant 6%, pH buffer (Tris buffered saline solution) 18%, phenol 3%, sodium chloride 5%, Formaldehyde 2%, mucus digestion components 0.5%, red blood cell lysate 0.5%

[0037] In this embodiment, the application of the cell preservation solution and the preparation method are as follows:

[0038] (1) Take 15ml of preservation solution and put it in a 50ml preservation bottle for later use;

[0039] (2) After the gynecologist clinically collects the sampling swabs, place them in a container containing a preservation solution;

[0040] (3) The medical technician enters the sample number and information, shakes for 5 minutes, and stands still for 30 seconds;

[0041] (4) Transfer 8 ml of cell solution to a disposable test tube; put the test tube into a centrifuge and centrifuge at 2000r / min for 5 minutes;

[0042] (5) Discard 1 / 2 of the supernatant a...

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Abstract

The invention relates to a cervical exfoliated cell preservation solution with a pretreatment function and application, and in particular to a cervical exfoliated cell preservation solution which contains cell fixative, anticoagulant, PH buffer, humectant, osmotic pressure-maintaining agent, microbe inactivator, mucus digestion ingredient and red blood cell treatment ingredient. The preservation solution can effectively preserve cervical exfoliated cells, protect the integrity and fixity of cells and prevent the pycnosis, cytolysis and distending of cells; the mucus treatment capability and the blood cell disintegration capability are high; the cell preservation time is up to 30 days; the slide preparation effect is flat and even, and preserved cells can also be applied in the preparationof cell wax blocks, HPV virus detection and the like; the effective period of the preservation solution is two years under normal temperature, the microbe killing capability is high, and the health ofmedical technicians is effectively protected.

Description

technical field [0001] The invention relates to a preservation solution for cervical exfoliated cells with pretreatment function and its application. Background technique [0002] The cell preservation solution can preserve the exfoliated cells obtained after sampling from a living body, and protect the integrity and fixity of the exfoliated cells. The purpose of preserving cells is to preserve the original structure of the cells as completely as possible, to coagulate or precipitate the components in the cells, to avoid degradation, autolysis, corruption and deformation of the cells, to inactivate various enzymes in the cells, and to prevent Various molecules of cells are denatured and dissociated, so that the chemical substances and enzymes of cells can be accurately positioned, and they will not be changed or damaged in the subsequent processing and production process. At the same time, each part of the cell can be easily colored, which is suitable for observation, long-...

Claims

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Application Information

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IPC IPC(8): A01N1/02G01N1/28G01N1/30
CPCA01N1/021A01N1/0226G01N1/2813G01N1/30
Inventor 姚斌左露露
Owner 南京福怡科技发展股份有限公司
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