Cervical exfoliated cell preservation solution with pretreatment function and application

Abstract

The invention relates to a cervical exfoliated cell preservation solution with a pretreatment function and application, and in particular to a cervical exfoliated cell preservation solution which contains cell fixative, anticoagulant, PH buffer, humectant, osmotic pressure-maintaining agent, microbe inactivator, mucus digestion ingredient and red blood cell treatment ingredient. The preservation solution can effectively preserve cervical exfoliated cells, protect the integrity and fixity of cells and prevent the pycnosis, cytolysis and distending of cells; the mucus treatment capability and the blood cell disintegration capability are high; the cell preservation time is up to 30 days; the slide preparation effect is flat and even, and preserved cells can also be applied in the preparationof cell wax blocks, HPV virus detection and the like; the effective period of the preservation solution is two years under normal temperature, the microbe killing capability is high, and the health ofmedical technicians is effectively protected.

Description

technical field [0001] The invention relates to a preservation solution for cervical exfoliated cells with pretreatment function and its application. Background technique [0002] The cell preservation solution can preserve the exfoliated cells obtained after sampling from a living body, and protect the integrity and fixity of the exfoliated cells. The purpose of preserving cells is to preserve the original structure of the cells as completely as possible, to coagulate or precipitate the components in the cells, to avoid degradation, autolysis, corruption and deformation of the cells, to inactivate various enzymes in the cells, and to prevent Various molecules of cells are denatured and dissociated, so that the chemical substances and enzymes of cells can be accurately positioned, and they will not be changed or damaged in the subsequent processing and production process. At the same time, each part of the cell can be easily colored, which is suitable for observation, long-...

AI Technical Summary

Problems solved by technology

[0003] 1. The mucus in the mucus sample affects the precipitation and absorption of cells, which may easily cause false negatives;

[0004] 2. Bloody samples were not processed thoroughly, and red blood cells interfered with interpretation;

[0005] 3. Cells dissolve or swell, and the cell preservation effect is poor, which may easily lead to misjudgment;

[0006] 4. There are many overlapping and grouping of cells, and the production effect cannot be evenly tiled.

[0007] In addition, imported preservation solutions are often used in clinical practice, which is costly and unfavorable for popularization and promotion.

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