Method for preparing glycolysis Chinese herbal medicine preparations for feed

A technology for Chinese herbal medicine and forage, which is applied in the field of preparation of new-type fermented Chinese herbal medicine preparations, can solve the problems of easy inactivation of feed microorganism additives, ineffective resistance to hydrochloric acid, and difficulty in forming dominant flora, etc., so as to improve the flavor of muscle tissue. , save feed, promote the effect of rapid growth

Inactive Publication Date: 2008-02-06
济南亿民动物药业有限公司
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] ① Feed microbial additives are prone to inactivation during transportation, use and storage;
[0006] ② Some strains of microbial additives for feeding cannot effectively resist the effects of hydrochloric acid and bile acid when they enter the digestive tract, so they cannot colonize the intestinal tract and play a role;
[0007] ③ There are still few strains of feed microbial additives currently used, only a few strains such as Lactobacillus, Bifidobacterium, Fecal (feces) Streptococcus and yeast;
[0008] ④ After the live bacteria preparation enters the intestinal tract, its growth rate is slow, it is difficult to be in a dominant position in the microbial competition, and it is difficult to form a dominant bacterial group

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1: the preparation of fermented Chinese herbal medicine preparation for feeding, the specific operation steps are as follows:

[0053] A. Preparation of strains

[0054] 1) Preparation of Lactobacillus acidophilus (ACCC10637) strain

[0055] Take out the strain preservation tube, scratch the plate with MRS solid medium for recovery, and incubate anaerobically at 37°C for 48h. Pick a single colony on a plate and inoculate it into 50ml MRS liquid medium, and culture it anaerobically at 37°C for 24h in a shaker. The seeds were inoculated with 2% inoculum, and placed in a Erlenmeyer flask for anaerobic shaking at 37°C for 24 hours after inoculation.

[0056] 2) Preparation of Bacillus subtilis (ACCC11060) strain

[0057] Take out the strain preservation tube, use nutrient gravy agar medium to scratch the plate for recovery, and culture it anaerobically at 37°C for 48h. Pick a single colony on a plate and inoculate it into 50ml nutrient broth agar medium, and ...

Embodiment 2

[0065] Embodiment 2: the preparation of fermented Chinese herbal medicine preparation for feeding, the specific operation steps are as follows:

[0066] A. Preparation of strains

[0067] 1) Preparation of Lactobacillus acidophilus (ACCC10637) strain

[0068] Take out the strain preservation tube, scratch the plate with MRS solid medium for recovery, and incubate anaerobically at 37°C for 48h. Pick a single colony on a plate and inoculate it into 50ml MRS liquid medium, and culture it anaerobically at 37°C for 24h in a shaker. The seeds were inoculated with 2% inoculum, and placed in a Erlenmeyer flask for anaerobic shaking at 37°C for 24 hours after inoculation.

[0069] 2) Preparation of Candida utilis (ACCC20060) strain

[0070] Take out the strain preservation tube, use the wort juice agar medium for slant culture and seed culture, use 2% inoculum amount for the seeds, and put them in the Erlenmeyer flask for 24 hours with shaking at 37°C after inoculation.

[0071] B....

Embodiment 3

[0076] Embodiment 3: the preparation of fermented Chinese herbal medicine preparation for feeding, the specific operation steps are as follows:

[0077] A. The preparation of bacterial classification is the same as that in Example 2.

[0078] B. Preparation of Chinese herbal medicinal water extract for fermentation

[0079] Soak 40kg of Radix Astragali, 40kg of Radix Isatidis, 20kg of Epimedium plus 12 times of drinking water for 12 hours, extract in a steam negative pressure extraction tank for 3 hours, pump the medicinal liquid to a static precipitation tank, let it settle for 24 hours, and take the supernatant Concentrate under pressure to 1:1 (water ratio W / W).

[0080] C. Preparation of Fermentation Cultures in Seed Tanks and Fermentation Tanks

[0081] 1) The liquid medicine obtained in step B is added into the seed tank and the fermenter respectively at a ratio of 15% (water medicine ratio W / W), and sterilized.

[0082] 2) Inoculate the two kinds of bacteria obtained...

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Abstract

A preparation method of a ferment Chinese Traditional Herbs for feeding belongs to the production field of the feed additives. The technical proposal provided by the present invention is that one or various intestinal probiotics is inoculated on a cultivation matrix containing extracts of Chinese Traditional Herbs, and undergoes ferment cultivation in a proper condition. The probiotics include Lactobacillus acidophilus, Bacillus subtilis, and candida utilis, etc, and the selected Chinese Traditional Herbs include astragalus, atractlylis lancea formalyrata, codonopsis pilosula, pericarpium citri reticulatae, and atractylis atractylis ovata, etc. The ferment Chinese Traditional Herbs for feeding provided by the present invention used to breed the livestock and the poultry is capable of preventing and treating epidemic diseases and improving the growth of the animal. The biological ferment of the water extract of Chinese Traditional Herbs by the probiotics is utilized to produce the elements with higher activities, thereby improving the absorption, transportation, metabolism, activation and medical efficacy of the Chinese Traditional Herbs in human body.

Description

1. Technical field [0001] The invention relates to a preparation method of a novel feed fermented Chinese herbal medicine preparation, which belongs to the field of feed additive production. 2. Background technology [0002] With the development of animal husbandry and aquaculture and the improvement of people's living standards, people's understanding of food safety has gradually deepened, and the demand for the production of pollution-free, safe and green livestock, poultry and aquatic products is getting higher and higher. In 2001, the Chinese government officially promulgated and implemented the "China Food and Nutrition Development Program (2001-2010)", emphasizing the necessity of food safety. Food is an important material basis for human survival and development, and the quality and safety of food has become one of the focuses of global attention. In addition, although my country's livestock, poultry and aquatic products have a relatively large price advantage in the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/14A23K1/16A23K10/12A23K10/18A23K10/30A23K50/80
CPCY02A40/818Y02P60/87
Inventor 牛钟相王涛谢建章韩春杨
Owner 济南亿民动物药业有限公司
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